• Title/Summary/Keyword: Phryma leptostachya var. asiatica

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Isolation of Constituents with Nitric Oxide Synthase Inhibition Activity from Phryma leptostachya var. asiatica

  • Kim, Donghwa;Lee, Sang Kook;Park, Kyoung-Sik;Kwon, Na-Yun;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.25 no.1
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    • pp.34-37
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    • 2019
  • Phytochemical studies were performed to identify the active principles of Phryma leptostachya var. asiatica (Phyrymaceae) for anti-inflammation. The anti-inflammatory activity was assessed by measuring the inhibition rate on nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells. Of the five compounds including ursolic acid, phrymarolin I, harpagide, haedoxancoside A, and acteoside isolated from this plant, ursolic acid showed the most prominent inhibition of NO formation. Therefore, ursolic acid may be the anti-inflammatory principle of Phryma leptostachya var. asiatica.

Anti-Inflammatory, Antioxidant, Anti-Angiogenic and Skin Whitening Activities of Phryma leptostachya var. asiatica Hara Extract

  • Jung, Hyun-Joo;Cho, Young-Wook;Lim, Hye-Won;Choi, Hojin;Ji, Dam-Jung;Lim, Chang-Jin
    • Biomolecules & Therapeutics
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    • v.21 no.1
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    • pp.72-78
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    • 2013
  • This work aimed to assess some pharmacological activities of P. leptostachya var. asiatica Hara. The dried roots of P. leptostachya var. asiatica Hara were extracted with 70% ethanol to generate the powdered extract, named PLE. Anti-angiogenic activity was detected using chick chorioallantoic membrane (CAM) assay. In vitro anti-inflammatory activity was evaluated via analyzing nitric oxide (NO) content, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and reactive oxygen species (ROS) level in the stimulated macrophage cells. Matrix metalloproteinase-9 (MMP-9) and -2 (MMP-2) activities in the culture media were detected using zymography. PLE exhibits an anti-angiogenic activity in the CAM assay, and displays an inhibitory action on the generation of NO in the LPS-stimulated macrophage cells. In the stimulated macrophage cells, it is able to diminish the enhanced ROS level. It can potently scavenge the stable DPPH free radical. It suppresses the induction of iNOS and COX-2 and the enhanced MMP-9 activity in the stimulated macrophage cells. Both monooxygenase and oxidase activities of tyrosinase were strongly inhibited by PLE. Taken together, the dried roots of P. leptostachya var. asiatica Hara possess anti-angiogenic, anti-inflammatory, antioxidant and skin whitening activities, which might partly provide its therapeutic efficacy in traditional medicine.

Screening of Biologically Active Compounds from various weeds (다양한 잡초로부터 생리활성물질의 탐색)

  • Kang, Byeong-Hoa;Ryoo, In-Ja;Park, Dong-Jin;Lee, Hyun-Sun;Kim, Young-Ho;Yoo, Ick-Dong;Kim, Chang-Jin
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.409-413
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    • 1996
  • To search for bioactive compounds from plant resources, 80% methanol extracts of 46 species of weeds were screened for their activities of antimicrobial, antioxidative, antiblebing, antitumor and herbicidal. Among extracts tested, some showed activities at the concentration of $50\;to\;100\;{\mu}g/ml$. Phryma leptostachya var. asiatica, Aster ageratoides, Centipeda minima, Cirsium pendulum, Lythrum anceps showed antibacterial activity. Penthorum chinense, Lindernia procumbens, Aster ageratoides, Dianthus superbus var. longicalycinus showed antiblebing activity. Phyma leptostachya var. asiatica, Juncus effusus var. decipiens, Lindernia procumbens, Aster ageratoides, Dianthus superbus var. longicalycinus, Viscum album var. coloratum showed antitumor activity. Juncus effusus var. decipiens, Hypericum ascyron, Juncus papillosus, Inula britannicar var. chinensis, Scirpus wichurae, Hypericum laxum showed antioxidant activity.

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Phytosociological Characteristics of Qeurcus acutissima Forest in Daecheong-dam basin (대청댐 유역 상수리나무림의 식물사회학적 특성)

  • Kim, Sung-Yeol;Moon, Geon-Soo;Lim, Sung-Been;Paek, Hye-Jung;Song, Won-Kyong;Choi, Jae-Yong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.24 no.2
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    • pp.85-102
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    • 2021
  • Phytosociological characteristics on Quercus acutissima forests distribution in Daechong-dam basin survey has been carried out using Z.-M. School's methodology and numerical-classification analyses. A total of 43 phytosociological relevés were sampled. Syntaxa were described as Oplismenus undulatifolius-Quercus acutissima community(typicum subcommunity, Phryma leptostachya var. asiatica subcommunity, Ulmus davidiana var. japonica subcommunity), Quercus acutissima community and Quercus variabilis-Quercus acutissima community (typicum subcommunity, Castanea crenata subcommunity). The above three plant communities were classified with species composition reflecting local environmental characteristics of mountain topographies, inclination degrees, and rock exposure rates. Conclusively, those communities were recognized as secondary vegetation affected by high intensity and frequency of human impacts as they inhabited in southward hill lands and low lying grounds in mountains adjacent to human settlements and arable lands. Quercus acutissima community was classified as rural type syntax based on their inlandward distribution and species composition differences from urban forests. Afforest process and natural succession were discussed in relation with habitat environmental elements of Quercus acutissima forest in the survey area.

Analysis of Vegetation and Vegetation-Environment Relationships in Main Wild Vegetables of Ulleungdo in Korea -Vegetation of herb layer of the Aster glehni, Allium ochotense, and Aruncus sylvester - (울릉도 주요 산채류 자생지의 식생 및 환경과의 상관관계 분석 -섬쑥부쟁이, 울릉산마늘, 눈개승마의 초본층 식생을 중심으로-)

  • Lee, Joong-Ku;Kim, Hyoun-Sook;Lee, Sang-Myong;Park, Gwan-Soo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.6
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    • pp.71-82
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    • 2018
  • This study was conducted to provide ecological basic data that use to establish environmental conditions for cultivation of wild vegetables in 2016-2018. Therefore, we investigated the vegetation structure and the correlation between the community structure and the environmental factors for natural habitats of wild vegetables(Aster glehni, Allium ochotense, and Aruncus sylvester) distributed in Ulleungdo. As a result of population and gradient analysis, the vegetation was classified into Aster glehni community, Allium ochotense community, and Aruncus sylvester community. We confirmed that the classification by population analysis was consistent with that by TWINSPAN method, suggesting that they were complemented each other. The importance value of Aster glehni was the highest in all communities, followed by Aruncus sylvester, Allium ochotense, Hydrangea petiolaris, Dryopteris crassirhizoma, Asperula ldorata, Phryma leptostachya var. asiatica, Disporum viridrescens, Hedera rhombea, Anthriscus sylvestris, and Hepatica maxima. According to the results of DCCA ordination analysis, among those communities, the Aster glehni community was distributed in soil where the nutrition including T-N and O.M. were intermediate. The Allium ochotense community was distributed on the a little high northern slope at the highest altitude where the CEC and O.M. were the highest, and other nutrition and pH were low. The Aruncus sylvester was distributed on high slope and altitude on which the amount of exchangeable cation such as $Ca^{{+}{+}}$, $Mg^{{+}{+}}$ and pH were high, and the CEC, $P_2O_5$, and O.M. were the lowest.

Identification and Characterization of Three Isolates of Cucumber mosaic virus Isolated from Weed Hosts (잡초에서 분리한 3종 Cucumber mosaic virus의 동정과 특성)

  • Lee, Hyeok-Geun;Kim, Sung-Ryul;Jeon, Yong-Woon;Kwon, Soon-Bae;Ryu, Ki-Hyun;Choi, Jang-Kyung
    • Research in Plant Disease
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    • v.14 no.1
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    • pp.15-20
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    • 2008
  • Three isolates of Cucumber mosaic virus (CMV) were isolated from weed hosts showing typical mosaic symptoms, and some properties of the viruses were investigated. CMV isolates, designated as Is-CMV, Jd-CMV and Pla-CMV from Isodon inflexus, Jeffersonia dubia and Phryma leptostachya var. asiatica, respectively, were identified and characterized by biological reaction in several host plants, serological property, dsRNA analysis, reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment-length polymorphism (RFLP). All isolates systemically infected in Nicotiana benthamiana, Cucurbita pepo cv. Black beauty and Cucumis sativus, and did not reveal any differences in these host plants between the isolates. However, remarkable difference in the symptoms was found between the CMVs in Capsicum annuum. Is-CMV induced an asymptomatic symptoms, while Jd-CMV and Pla-CMV produced severe mosaic symptoms in C. annuum plants. In dsRNA analysis, all isolates revealed four major bands with estimated molecular size of 3.4, 3.2, 2.1 and 1.0 kbp. The cDNAs of coat protein gene of the isolates were amplified by RT-PCR using a genus-specific single pair primers that designed to amplify a DNA fragment of approximately ranging from 938 to 966 bp. By restriction mapping analysis using RFLP of the RT-PCR products as well as by serological properties of gel diffusion test, the CMV isolates belong to a typical members of CMV subgroup IA. This is the first report on the occurrence of CMV in the three weed hosts.

Habitats Environmental Characteristics of Polypodium vulgare L. in Ulleung-do (울릉도 미역고사리(Polypodium vulgare L.) 자생지의 입지환경특성)

  • Cheon, Kyeong-Sik;Han, Jun-Soo;Kim, Kyung-Ah;Ok, Kil-Hwan;Yoo, Ki-Oug
    • Korean Journal of Environment and Ecology
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    • v.26 no.1
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    • pp.1-10
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    • 2012
  • The habitats characteristics of Polypodium vulgare L. in Ulleung-do were investigated to compile basic data for conservation and restoration. Natural habitats were located at altitudes of 410~748m with inclinations of $12{\sim}80^{\circ}$. Sixty six vascular plants were identified from 10 quadrats in 4 habitats. Dominant species among the woody plants, based on importance value, were Acer pictum subsp. mono(49.52%) in the tree (T1) layer, Sorbus amurensis(28.99%) in the subtree (T2) and Schizophragma hydrangeoides(51.99%), Ligustrum foliosum(8.82%), Fagus engleriana(7.25%) in the shrub (S) layer. Importance value for members of the herb (H) layer were as follows: Polypodium vulgare 23.23%; Maianthemum dilatatum 9.65%; Phryma leptostachya var. asiatica 9.23%; Dryopteris crassirhizoma 8.40%; Carex shimidzensis 6.75% and Dystaenia takesimana 5.42%. The importance value of the last five species were high, so they were at affinity with Polypodium vulgare in their habitats. Species diversity was 1.18, and dominance and evenness were found to be 0.11 and 0.84, respectively. The soil types were sandy loam. Average field capacity was 30.42%, and the organic matter and pH were 17.95%, and 4.70. Correlation coefficients based on environmental factors, vegetation and soil analysis were showed that positive correlations between species diversity and species richness, whereas between species diversity and dominance, coverage of Polypodium vulgare and species richness were showed negative correlations.