• Journal of Environmental Science International
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• v.25 no.4
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• pp.473-481
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• 2016

We examined the effects of ocean acidification (OA) and eutrophication on the physiology of a red alga, Gracilariopsis chorda, using specimens collected at Wando Island, Korea, in July of 2015. The samples were transported to a laboratory and placed on growth media for treatments involving low or high levels of ammonium ($4{\mu}M$ or $60{\mu}M\;NH_4{^+}$) and low or high pH(7.5 or 8.2). The control treatment used filtered seawater (pH 8.2 and $4{\mu}M\;NH_4{^+}$). All experiments were conducted at $20^{\circ}C$ and under a lighting intensity of $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$, with or without an injection of $CO_2$ (pH 7.5). In addition, we calculated rates of respiration under darkness, at a pH of 7.5 and $60{\mu}M\;NH_4{^+}$. Fluctuations in pH as well as the evolution of photosynthetic oxygen and $NH_4{^+}$ uptake rates were monitored for 6 h. The greatest increase in pH levels, from 7.50 to 8.65, occurred in response to $60{\mu}M\;NH_4{^+}$, whereas the largest decrease, from 7.50 to 7.42, was associated with elevated respiration rates. At a pH of 7.5, rates of oxygen evolution were higher (236% saturation) for samples treated with $60{\mu}M\;NH_4{^+}$ than for the control (121% saturation). Ammonium uptake was highest at pH 7.5 and $60{\mu}M\;NH_4{^+}$, with a rate of $0.526{\pm}0.002{\mu}mol\;g^{-1}\;FW\;h^{-1}$, followed in order by the treatments of $pH\;8.2/60{\mu}\;NH_4{^+}$, $pH\;7.5/4{\mu}M\;NH_4{^+}$, and the control ($pH\;8.2/4{\mu}M\;NH_4{^+}$). We speculated that the rates of photosynthesis and $NH_4{^+}$ uptake could be enhanced at a higher ammonium concentration and lower pH because $CO_2$ concentrations were increased through greater photosynthetic activity. Therefore, these findings suggest that the physiology of G. chorda populations can be improved by the interaction of optimized $CO_2$ concentrations and an adequate supply of essential nutrients such as ammonium.

• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.236-243
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• 2012

The leaf growth and physiological characteristics of Forsythia saxatilis were investigated at six natural habitats under different light intensities in Bukhansan in order to figure out an appropriate growth environment for conservation and restoration of F. saxatilis that is Korean endemic plant designated as rare and endangered species. Relative light intensities (RLI) at six habitats showed from 10% to 78% of the full sun. Leaf length, leaf width, leaf area and dry leaf weight of population under highest relative light intensity (78%) were the highest. The ratio of dry leaf weight to leaf area increased with the increase of RLI. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were the highest at 78% of RLI, whereas the ratio of total chlorophyll to carotenoid content reduced according to the increase of RLI. Photosynthetic parameters, such as photosynthetic rate, also increased with the increase of RLI. The total nonstructural carbohydrate (TNC) was 1.5 times higher at 78% of RLI than that at 10% of RLI and the total soluble sugar (TSS) was the highest at 78% of RLI. In conclusion, leaf characteristics and physiological characteristics have high positive correlation with light intensity. Therefore, light condition should be primarily considered to improve growth and physiology characteristics of F. saxatilis under low light intensity.

• Journal of The Korean Association For Science Education
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• v.35 no.3
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• pp.419-429
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• 2015

In this study, we explored students' epistemological framing during scientific argumentation and how interactions among group members influenced group argumentation. Twenty-one gifted science students divided into groups of three or four participated in this study. Students' discussions related to data interpretation concerning the rate of photosynthesis were analyzed. Students' activities were videotaped in groups so the discourse could be transcribed and students' behavioral cues analyzed. Students' epistemological framing has been identified through analysis of their speech and behavioral responses to the anomalous data from the inquiry process. Subsequently, their sources of warrant and group argumentation levels were explored. We found out that group members framed the inquiry in two ways: "understanding phenomena" and "classroom game." Group members whose framing was "understanding phenomena" required other members to justify the anomalous data by examining its validity and reliability, which conclusively demonstrated a high level of argumentation. On the other hand, when group members used "classroom game" to frame their argumentation, they did not recognize the necessity of explaining the anomalous data; rather, these students used simple empirical justification to explain the data, reflecting a low level of argumentation. When students using different epistemological framing disagreed over interpretations of anomalous data throughout the discussion, clashes ensued that resulted in emotional conflict and a lack of discussion. Students' framing shifts were observed during the discussion on which group leaders seemed to have a huge influence. This study lays the foundation for future work on establishing productive framing to prompt scientific argumentation in science classrooms.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.138-142
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• 2008

To develop suitable alternative substrates in hydroponics of sweet pepper, changes of water contents of substrates and electric conductivity (EC) of drainage nutrient solution, growth and fruit characteristics according to the kind of coir substrates were investigated compared with rockwool. In all coir substrates compared with rockwool during growing period, water contents were higher, EC of drainage nutrient solution except for fiber 50% were lower, and the coefficient of variation were a little, especially were so in fiber 30% of coir substrates. Plant growth in fiber 30% substrate was better than others but there was no significant difference. Photosynthesis rate and chlorophyll content were best in fiber 50% substrate. Fruit weight was no significant difference, but LID ratio and shape of the fruits were good in fiber 50% substrate and rockwool as which were close to regular square. Therefore, it was estimated that mixed coir substrates with fiber $30{\sim}50%$ are enough in possibility as alternative substrate.

• Research in Plant Disease
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• v.22 no.4
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• pp.257-263
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• 2016

Chlorella is known as chlorophyceae which can live autotrophically by photosynthesis, promote the growth of plants and suppress some plant diseases. However, a few researches in inhibition mechanism of plant diseases by chlorella have been carried out. In this study cucumber leaves pre-treated with Chlorella fusca suspension were investigated whether anthracnose by Colletotrichum orbiculare is suppressed or not. Furthermore, in order to illustrate how the algae can restrain the antracnose, the infection structures of C. orbiculare were observed on the cucumber leaves pre-treated with the algae. Consequently, appressorium formation rate was apparently reduced in the cucumber leaves pre-treated with C. fusca compared to untreated control one. Also, the numbers of conidia found at the inoculation sites were significantly reduced compared to untreated one. On the other hand, on the leaves pre-treated with $Benomyl^{(R)}$ appressorium formation were decreased remarkably and numbers of conidia were also reduced similar with those pre-treated with C. fusca. Based on these results, it was revealed that occurrence of anthracnose can be suppressed by C. fusca pre-treatment and suggested that biochmical or structural hinderance by C. fusca resulting in the decline of appressorium formation on the leave surfaces may play an important role in the disease suppression.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.4
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• pp.180-191
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• 2015

Marine environmental characteristics and primary productivity of phytoplankton were investigated in seaweed bed of northwestern coast of Jeju Island during Autumn, 2014. The trophic state based on dissolved inorganic nitrogen and phosphorus was mesotrophic. The Redfield ratio was less than 16, indicating that nitrogen was the limiting factor for the growth of phytoplankton. Dissolved organic nitrogen and phosphorus accounts for 63 and 46% of the dissolved total nitrogen and phosphorus, respectively. Light utilization efficiency (${\alpha}$) and maximum photosynthetic capacity ($P_m{^B}$) were highest in the Donggwi (third-year marine forest), followed by Gonae (one-year marine forest), Biyangdo (natural seaweed bed) and Geumneung (whitening area). The primary productivity of phytoplankton in the Donggwi, Gonae and Biyangdo also was higher than that in the Geumneung. Although nitrogen is the limiting factor, enriched dissolved organic nitrogen might play an important role to maintain primary productivity. In addition, phytoplankton community through photosynthesis could produce about 14% of phytoplankton carbon in one hour. These results will be able to use the important information for material cycle and ecological valuation of seaweed bed.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.325-331
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• 2013

This experiment has been carried out to determine optimal culture conditions for the production of cut flowers and tubers of Calla (Zantedeschia 'Golden Affair' and 'Black Magic') in highlands. Treatments consisted of various levels of root zone environments, Results are as follows: Calla 'Golden Affair' were grown with different mulching materials such as Non-mulching, Black film, reflective film, Rice hull. Mulching materials resulted in no difference in the number of cut flowers but flower length was highest in reflective film. Calla 'Black Magic' were treated with various soil water content, soil type and watering time. Number of cut flowers and flower quality were greatest when the plants were watered at -80 kPa soil water content. No symptoms of soft rot (Erwinia carotovora) was observed at this soil water content. The occurrence of soft rot was observed with similar percentage according to soil type and the soil water content. When Calla 'Black Magic' were watered at the time of soil surface drying, growth was greater compared to others. Air temperature and PPF affected plant growth and photosynthesis. Photosynthetic rate was greatest at $25^{\circ}C$ and PPF $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, while lowest at $28^{\circ}C$ and PPF $800{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Soft rot did not occur without regard to dipping treatment (0, 5, 10, 24, 48 hours) when the day and night temperature were maintained at $25^{\circ}C$ and $20^{\circ}C$, respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.159-159
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• 2017

Drought, a common environmental constraint, induces a range of physiological, biochemical and molecular changes in plants, and can cause severe reductions in crop yield. Consequently, understanding the molecular mechanisms of drought tolerance is an important step towards crop biotechnology. Here, we report that the rice (Oryza sativa) homeodomain-leucine zipper class IV transcription factor gene, ${\underline{R}ice}$ ${\underline{o}utermost}$ ${\underline{c}ell-specific}$ gene 10 (Roc10), enhances drought tolerance and grain yield by increasing lignin accumulation in ground tissues. Overexpression of Roc10 in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both more effective photosynthesis and a reduction in water loss rate, compared with non-transgenic controls or RNAi transgenic plants. Importantly, Roc10 overexpressing plants had a higher drought tolerance at the reproductive stage of growth and a higher grain yield compared with the controls under field-drought conditions. Roc10 is mainly expressed in outer cell layers including the epidermis and the vasculature of the shoots, which coincides with areas of cell wall lignification. Roc10 overexpression elevated the expression levels of lignin biosynthetic genes in shoots, with a concomitant increase in the accumulation of lignin, while the overexpression and RNAi lines showed opposite patterns of lignin accumulation. We identified downstream target genes of Roc10 by performing RNA-seq and chromatin immunoprecipitation (ChIP)-seq analyses of shoot tissues. Roc10 was found to directly bind to the promoter of PEROXIDASEN/PEROXIDASE38, a key gene in lignin biosynthesis. Together, our findings suggest that Roc10 confers drought stress tolerance by promoting lignin biosynthesis in ground tissues.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.221-228
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• 1994

Respitation and growth rates of leaves, branches, stems and roots of 2 to 11-yr-old red pine trees(Pinus densiflora) were examined and applied to Thornley's growth equation, $$R=(\frac{1-Yg}{Yg})\frac{dW}{dt}+mW$$. The conversion efficiency of substrates(Yg), maintenance respiration coefficients(m), relative growth rates(${\mu}$) were estimated. The efficiency of conversion of substrates (Yg) was 0.3637g/g dw/yr and the maintenance respiration coefficient(m0 was 0.094g/g dw/yr. The relative growth rate(${\mu}$) was remarkably reduced with age from 0.90(2-year-old) to 0.33:11 year-old). The Ratio of gross respiration(R) per gross photosynthesis(Pg), R/Pg showed the range of 0.6~0.7 and annually 64% of Pg was spent for constructive respiration. The 3.4% of dry weight of whole tree was spent for maintenance respiration.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.325-334
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• 1996

Pepper plants were flooded at 0, 5, 10 and 15cm at seedling stage under the condition of greenhouse. Treatment of flooding times are 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height, number of leaves, shoot and root fresh weight were not recovered the flooding damages regardless of flooding time and depth. Pepper plant died in flooding depth of 5cm or more for over 48 hours. Plants in fallen leaves were found at more than 5cm depth and 6 hours of floodings. Photosynthesis and respiration rate decreased in the 5cm flooding depth or more for 24 hours. Chlorophyll content and root activity decreased for 12 hours or more at all the flooding depth. Also, diffusion resistance of stomata cell increased as increased flooding time and depth. Diseases occurred remarkably in proportion to the depth and hours of flooding treatment. It was not possible to control the desease by fungicide, also then was no effects of foliar spray of urea. Weight of fruit per plant not decrease by the 12 hours of 0cm and the 6 hours of 5cm flooding but decreased at deeper and longer flooding. Average weight of a fruit increased. The yield could not expected in the depth of 5cm or more for over 48 hours, There was significant positive correlation between all the investigated characteristics of growth and yield. There was, however, negative correlation between number of leaf and diffusion resistance of stomata.