• The Korean Journal of Mycology
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• v.44 no.3
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• pp.188-192
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• 2016

A previously unrecorded fungus was isolated from the persimmon (Diospyros kaki) leaf phyllosphere in Korea. The isolated fungus was characterized by morphological and phylogenetic analyses. The typical morphological characteristics of Phialocephala piceae, including dark brown colonies and short, thick conidiophores, were observed on the isolated fungus. A phylogenetic analysis based on the internal transcribed spacer (ITS) region and RNA polymerase II largest subunit (RPB1) also confirmed the identification of the isolated fungal species as P. piceae. Therefore, this is the first report of P. piceae in Korea.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.132-137
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• 2016

A unrecorded hyphomycete species of Phialocephala was isolated for the first time during the investigation of fungal community in the soil samples collected from different regions of Korea. The fungal isolate was identified as Phialocephala lagerbergii, based on the morphological characteristics and phylogenetic analysis of the ribosomal DNA sequence. In addition, cultural and micro-morphological features were described in detail.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.338-341
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• 2016

A dark septate endophytic fungal strain 13E043 was isolated from the needle leaves of Thuja koraiensis from a forest in Korea. Morphological characteristics of conidia and phialids. Along with a molecular phylogenetic analysis based on the internal transcribed spacer region of rDNA, the isolate was identified as Phialocephala piceae (also known as Phaeomollisia piceae). This is the first report of a dark septate endophyte isolated from the foliage of conifer trees in Korea.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.184-187
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• 2016

Endophytic fungi were isolated from surface-sterilized roots of a species of orchid, Oreorchis patens, in Korea. The isolated strains were identified based on morphological characteristics and sequence analyses of the internal transcribed spacer (ITS) and large subunit (LSU) of rDNA regions. Two species, Thelonectria veuillotiana and Phialocephala humicola, are first reported in Korea with their descriptions.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.355-361
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• 2017

In this study, endophytic fungi were isolated from the roots of three native orchid species from Korea: Bletilla striata, Oreorchis patens, and Cephalanthera longibracteata. The isolated fungal endophytes were identified based on the morphological and molecular characteristics including sequence analysis of internal transcribed spacer (ITS) and 28S ribosomal DNA regions. As a result, we discovered 5 species of fungal endophytes that have not been previously reported in Korea: Phialocephala bamuru, Coniochaeta mutabilis, Phialophora foetens, Calonectria canadensis, and Neonectria ramulariae.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.394-398
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• 2017

A survey to assess the diversity of wound-associated Ophiostomatales and Microascales, the so-called ophiostomatoid fungi, on Korean native trees, was undertaken in 2017. Wounds were artificially created, and a fungus resembling a species of Sporendocladia was consistently isolated from the exposed cambium and inner bark of artificially induced wounds on Quercus variabilis. Morphological examination and DNA sequence comparisons based on the internal transcribed spacer (ITS) and 5.8S regions of the rDNA confirmed the identity of the fungus as Sporendocladia bactrospora. This is the first report on S. bactrospora occurring on Q. variabilis in Korea.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.686-695
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• 2021

Tricholoma matsutake is an ectomycorrhizal fungus that has a symbiotic relationship with the root of Pinus densiflora. Soil microbial communities greatly affect the growth of T. matsutake, however, few studies have examined the characteristics of these communities. In the present study, we analyzed soil fungal communities from Gyeongju and Yeongdeok using metagenomic pyrosequencing to investigate differences in fungal species diversity, richness, and taxonomic composition between the soil under T. matsutake fruiting bodies (Sample 2) and soil where the fairy ring of T. matsutake was no longer present (Sample 1). The same spot was investigated three times at intervals of four months to observe changes in the community. In the samples from Yeongdeok, the number of valid reads was lower than that at Gyeongju. The operational taxonomic units of most Sample 2 groups were less than those of Sample 1 groups, indicating that fungal diversity was low in the T. matsutake-dominant soil. The soil under the T. matsutake fruiting bodies was dominated by more than 51% T. matsutake. From fall to the following spring, the ratio of T. matsutake decreased. Basidiomycota was the dominant phylum in most samples. G-F1-2, G-F2-2, and Y-F1-2 had the genera Tricholoma, Umbelopsis, Oidiodendron, Sagenomella, Cladophialophora, and Phialocephala in common. G-F1-1, G-F2-1, and Y-F1-1 had 10 genera including Umbelopsis and Sagenomella in common. From fall to the following spring, the amount of phyla Basidiomycota and Mucoromycota gradually decreased but that of phylum Ascomycota increased. We suggest that the genus Umbelopsis is positively related to T. matsutake.