• 한국균학회지
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• 제27권2호통권89호
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• pp.124-131
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• 1999

본 실험은 진흙버섯류 7종 15균주에 대한 5.85 rDNA와 ITS 부위의 염기서열을 비교 분석함으로서 종간 및 종내의 유연관계를 조사하였다. 5.8S rDNA와 ITS 부위를 증폭하고자 18S rDNA의 3'말단 부위와 28S rDNA의 5'말단 부위에 두 개의 primer를 이용하여 PCR증폭을 행하였다. 5.8S rDNA와 ITS 부위를 증폭하여 염기서열을 비교 분석한 결과 본 실험에 공시된 Phellinus속의 제균종은 크게 4개의 cluster를 형성하였다. 첫 번째 cluster는 Phellinus hartigii IMSNU 32041, Phellinus robustus IMSNU 32068로 이루어졌고, 두 번째 cluster는 Phellinus linteus KCTC 6190, IMSNU 31014, DGUM 25003, DGUM 25004, Phellinus sp. DGUM 25007, Namsan No. 1과 Phellinus weirianus IMSNU 32021, 세 번째 cluster는 Phellinus laevigatus KCTC 6229, KCTC 6230과 Phellinus igniarius KCTC 6227, KCTC 6228로 이루어졌으며, Phellinus chrysoloma KCTC 6225와 KCTC 6226이 마지막 cluster를 형성하였다. 결과적으로 ITS 염기서열의 결과만으로 볼 때 Phellinus linteus와 Phellinus weirianus는 명확하게 종 단위의 개념을 정립할 수 없었다. 따라서 정확한 분류를 위해 생리학적, 분자생물학적 인 분류방법이 첨가되어야 하며, type strain에 대한 ITS 염기서얼도 결정되어야 한다. Phellinus속의 균들에서는 ITS2부위에 비해 ITS1부위의 변이율이 높았다. ITS 염기서열은 종 구분에 유용한 도구이며, 다른 균종들과 비교해 보았을 때 Phellinus linteus와 Phellinus weirianus에서만 ITS1 부위에서 특이적인 염기서열을 가지고 있었다.

• Toxicological Research
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• 제20권1호
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• pp.37-42
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• 2004

본 연구는 Phellinus gilvus(PGE), Phellinus linteus (PLE) 그리고 Phellinus baumil(PBE)의 열수추출물에 대하여 SRB법과 MTT법으로 종양세포주(Sarcoma 180과 P388)를 이용하여 항암활성을 비교ㆍ평가하였다. 종양세포주는 PGE, PLE, PBE(7., 15, 30 ug/ml) 그리고 Doxorubicin(DOX)(0.001～10 mM)으로 처리되었다. 그 결과 DOX, PGE 그리고 PLE는 종양세포주에 대하여 농도의존적으로 억제하는 결과를 보였지만, PBEssm 30 ug/ml의 농도에서만 억제되는 항암활성을 보여주었다. 결론적으로 이 연구에서 사용된 PGE, PLE 및 PBE는 Sarcoma 180과 P388에 항암활성을 보여주었다. PLE는 sarcoma 180종양세포에 가장 효과가 뛰어났으나(p<0.05), SRB법에서는 PGE가 P388에 대해 가장 큰 항암활성을 나타내었다(p<0.05).

• 한국균학회지
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• 제27권5호
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• pp.337-340
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• 1999

rDNA내의 ITS region의 염기서열 분석 결과를 토대로 19종의 Phellinus 속균중 Phellinus linteus를 특이적으로 동정할 수 있는 primer를 제작하였다. 이 특이 primer는 ITS1과 ITS2내에 위치하며 이들 spacer region에 인접해 있는 universal Primer내에 위치해 있다. 총 4개의 Primer(universal primer인 ITS-1F와 ITS-4 그리고 특이 primer인 PL-F와 PL-R)가 한국에서 채집된 Phellinus 속균중 Phellinus linteus를 동정하는데 사용되었다. Phellinus linteus의 증폭된 DNA크기는 800bp(ITS-1F/ITS-4)와 720 bp(ITS-1F/PL-R과 PL-F/ITS-4)에 해당하는 2개의 band, 그리고 610 bp(PL-F/PL-R)인 것으로 나타났다. 한국에서 채집된 23종의 Phellinus속균중 13종이 Phellinus linteus인 것으로 확인되었다.

• 대한의생명과학회지
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• 제22권2호
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• pp.53-59
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• 2016

Alcoholism is a significant health problem in the world. The liver is the first and primary target organ for alcohol metabolism. Alcohol dehydrogenase and aldehyde dehydrogenase play important roles in the metabolism of alcohol and aldehyde. In this study, I aimed to investigate the eliminatory effects of a Phellinus spp. extract on alcohol metabolism in drunken Sprague-Dawley (SD) rats. Male SD rats were given Phellinus spp. extract at 30 min after 40% (5 g/kg) alcohol ingestion. To assay the effect of Phellinus spp. extract on blood alcohol concentration, blood samples were taken from the tail vein at 1, 3 and 5 h after alcohol ingestion. The concentrations of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase in Phellinus spp. extract treated rat were significantly lower than that of the control with a time-dependent manner. In addition, the alanine aminotransferase and aspartate aminotransferase activities of Phellinus spp. extract-treated groups were altered compared to those of the control group. These results suggest that Phellinus spp. extract intake can have a positive effect on the reduction of alcohol, alcohol dehydrogenase, and aldehyde dehydrogenase concentrations in the blood and may alleviate acute alcohol-induced hepatotoxicity by altering alcohol metabolic enzyme activities. Phellinus spp. extract is thus a good nutraceutical candidate.

• 대한약침학회지
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• 제6권3호
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• pp.57-63
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• 2003

Background : This study was to investigate existing studies on the mushroom Phellinus linteus for possible applications in treating hard to cure diseases and uses in herbal acupuncture as the above is known to have an anti-cancer effects and stimulating the immune system. Results : Based on the literary consideration, the following results were obtained : 1. Proteins and polysaccharides of the Class Basidiomycetes activate the host immune system for anti-cancer action without known side effects, thus it can be given for the prevention and treatment of cancer as a supplement. Phellinus linteus mushroom showed the most significant effects. 2. The Phellinus linteus mushroom is a fungi in the family Hymenochaetaceae and Phellinus, under the class Basidiomycetes. Phellinus linteus can be often found on the trunk of mulberry trees and other latifoliate trees. 3. The characteristics of Phellinus linteus are sweet, neutral in temperature, non-toxic, dissipate the stagnated blood, stop bleeding, invigorate the blood circulation and remove stasis, and nourish qi. It has been used for treating continuous menstrual discharge among the female patients and removing masses in the abdomen to name a few. 4. Phellinus linteus contains agaric acid, fatty acid, C23 and other numerous enzymes. 5. Phellinus linteus is known to have anti-cancer and anti-oxidant effects, stimulation of the immune system, as well as anti-inflammatory effects, inhibition of angiogenesis, and analgesic actions.

• Toxicological Research
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• 제19권2호
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• pp.147-152
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• 2003

We investigated antitumor activities of the ethanol extract from mushroom Phellinus linteus and Phellinus baumii on mulberry, oak and elm. in vitro test, the ethanol extract of mushroom cultivated on oak of Phellinus linteus showed highest activities about SK-OV-3, HCT15, XF498, SK-MEL-2 and A549. SK-OV-3 cell line showed 100% cytotoxicity in 100 $\mu\textrm{g}$/ml and HCT15 (98.39%), XF498 (89.62%), SK-MEL-2 (84.07%) and A549 (79.92%) cytotoxicity respectively. Also $IC_{50}$ showed 3.99 $\mu\textrm{g}$/ml to SK-OV-3 cell line and HCT15 (4.37 $\mu\textrm{g}$/ml), A549 (5.48 $\mu\textrm{g}$/ml), SK-MEL-2 (6.72 $\mu\textrm{g}$/ml), XF 498 (6.88 $\mu\textrm{g}$/ml). As those results, cultivated oak of Phellinus linteus showed a very low $IC_{50}$ value against SK-OV-3, HCT15, XF498, SK-MEL-2 and A549 cancer cell lines.

• 대한수의학회지
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• 제43권3호
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• pp.423-428
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• 2003

진흙버섯 품종별 P. linteus, P. baumii 그리고 P. gilvus에 대한 형태학적 차이, 일반성분 및 무기성분을 조사하였다. 전자현미경 사진에서 P. gilvus와 P. linteus는 비슷한 형태를 보여주고 있다. 원소 함량의 구성비에서는 세 종류의 버섯이 모두 유사함을 보여주었다. 일반성분을 분석 비교시 수분의 함량은 P. baumii가 가장 높았으며 P. gilvus의 수분 함량은 10% 이내로 낮은 경향을 보여주었다. 식이섬유는 약 30% 이상을 함유하고 있으나 vitamin C 및 단백질의 함량은 높지 않았다. 원소분석에서 Ca의 경우 세 종류의 진흙버섯에서 P. baumi가 가장 높은 Ca 함량을 보여주었으나 통계적유의성은 없었다. 그러나 원소조성 및 일반성분 비교에서는 P. linteus, P. baumii 그리고 P. gilvus의 큰 차이점이 나타나지 않았다.

• KSBB Journal
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• 제14권2호
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• pp.167-173
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• 1999

A new synthetic medium was developed for the submerged mycelial cultures of Phellinus linteus. The medium for maximum mycelial growth of Phellinus linteus (3 days incubation, 28$^{\circ}C$, pH 5) consisted of (per 1 L): glucose, 90 g peptone, 10 g soluble starch, 10 g yeast extract, 3 g KH2PO4, 1 g MgSO4.7H2O, 1 g and CaCl2, 0.1 g. The concentrations of glucose, peptone, yeast extract, KH2PO4, MgSO4.7H2O, and CaCl2 were examined in the ranges of 10~90 g/L, 0~10 g/L, 0~15 g/L, 0~2 g/L, 0~1 g/L, and 0~0.5 g/L, respectively. The dry weight of mycelium in 3 days increased to 16.79 mg/mL using the new synthetic medium. The optimum temperature for mycelial growth of Phellinus linteus was 28$^{\circ}C$. The concentrations of KH2OP4, CaCl2, and yeast extract, which gave the maximum mycelial growth of Phellinus linteus, existed in the concentration ranges examined in this study. But, in the cases of other compositions (MgSO4.7H2O, peptone, and glucose), the mycelial growth of Phellinus linteus increased with the concentration in the ranges.

• Mycobiology
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• 제30권4호
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• pp.202-207
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• 2002

This study was carried out to develop specific primers for PCR detection of Phellinus linteus. Diverse genomes of 15 Phellinus spp. including five Phellinus linteus isolates were fingerprinted by Primer Universal rice primer(URP)1F. The URP-PCR pattern differentiated P. linteus isolates from other phellinus spp. A polymorphic band(2.8 kb), which is unique for P. linteus isolates, was isolated and sequenced. Twenty four-oligonucleotide primer pairs were designed based on information of DNA sequence. The primer set(PLSPF2/PLSPR1) amplified single band(2.2 kb) of expected size with genomic DNA from seven Phellinus linteus, but not with that of other Phellinus species tested. The primers could be used identically in both DNA samples from mycelium and fruit bodies. This specific primers could offer a useful tool for detecting and identifying P. linteus rapidly.

• 대한본초학회지
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• 제32권4호
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• pp.47-52
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• 2017

Objective : Phellinus spp. mushroom is an object of interest because it has excellent anticancer effect. Owing to the similalarities in the morphology, Phellinus linteus and Phellinus baumii are often used as same Sang Hwang Mushroom in the Korean market.. The quality control for mushrooms is needed because there are many differences in the efficacy according to cultivation method and cultivation area. Therefore, a reliable authentication method of these herbal medicine is necessary to compare and measure the amount of beta-glucan which is known to have a hypoglycemic effect, from the mushrooms collected in various regions Methods : 7 samples of medicinal mushrooms supplying phellinus spp. were collected in Korea, China and Cambodia. We investigated the hardness, colors, extract ratio, ${\alpha}-amylase$ and ${\alpha}-glucosidase$ inhibitory activities, glucose transporter 4 (GLUT-4) expressions of water extracts from Phellinus spp and also MTT assay were examined for cell toxicity. Results : The results revealed that Phellinus spp.water Ext.inhibited ${\alpha}-glucosidase$ activity. glucose transporter 4 (GLUT-4), the key insulin signaling pathway transcription factor, was remarkably increased by the Phellinus baumii water extract Conclusions : These results suggest that The more yellowish the mushroom is, the lower the hardness, the more the content of ${\beta}-glucan$ is proportional. Because the more ${\beta}-glucan$, the greater the effect of hypoglycemia. compared to the hypoglycemic effect, Phellinus Baumii grown at hanging on selves for 7 month in the green house is the best.