• KSBB Journal
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• 제15권5호
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• pp.537-540
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• 2000

일반적으로 paclitaxel과 유사한 구조를 가진 복잡한 유도체의 분리나 최종 제품에서의 엄격한 규정 등으로 인하여 한 단계의 HPLC system으로 정 제하는 것은 상당히 어렵다. 이러한 이유로 두 단계 HPLC system으로 식물세포 배양액으로 부터 paclitaxel의 대량 정제를 수행하였다. 즉, 첫번째 단계에 서 reverse-phase HPLC column, 두번째 단계에서 normal­p phase HPLC column을 사용하여 최종 제품을 얻었다. 또한 최종 정 제 된 paclitaxel 내 에 포함되어 있는 불순물 profile 확 언, 함량 분석, 그리고 이들 물질들에 대한 분리, 정제 및 동 정을 실시하였다 이들 불순물들 중에서 0.1% 이상되는 것은 모두 6종 (side chain derivative, baccatin III, 10-deacetyltaxol, cephalomannine, taxane derivative, 7-epitaxol)이며 NMR과 MS를 사용하여 화학구조를 분석한 결과 이미 알려진 pacli­t taxel 유도체 또는 전구체와 동일한 물칠로 밝혀 졌다. 본 연구 결과는 결국 paclitaxel 생산을 위한 품질관리 (quality con­t tr이)와 허가를 위한 자료로 유용하게 사용되어 진다.

• Journal of Positioning, Navigation, and Timing
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• 제12권4호
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• pp.359-367
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• 2023

In order to ensure the high-integrity of reference stations of satellite navigation system, cycle slip should be precisely monitored and compensated. In this paper, we proposed a cycle slip algorithm for the integrity monitoring of the reference stations. Unlike the legacy method using the Melbourne-Wübbena (MW) combination and ionosphere combination, the proposed algorithm is based on ionosphere combination only, which uses high precision carrier phase observations without pseudorange observations. Two independent and complementary ionosphere combinations, Ionospheric Negative (IN) and Ionospheric Positive (IP), were adopted to avoid insensitive cycle slip pairs. In addition, a second-order time difference was applied to the IN and IP combinations to minimize the influence of ionospheric and tropospheric delay even under severe atmosphere conditions. Then, the cycle slip was detected by the thresholds determined based on error propagation rules, and the cycle slip was identified through weighted least square method. The performance of the proposed cycle slip algorithm was validated with the 1 Hz dual-frequency carrier phase data collected under the difference levels of ionospheric activities. For this experiment, 15 insensitive cycle slip pairs were intentionally inserted into the raw carrier phase observations, which is difficult to be detected with the traditional cycle slip approach. The results indicate that the proposed approach can successfully detect and compensate all of the inserted cycle slip pairs regardless of ionospheric activity. As a consequence, the proposed cycle slip algorithm is confirmed to be suitable for the reference station where real time high-integrity monitoring is crucial.

• 한국환경보건학회지
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• 제41권5호
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• pp.305-313
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• 2015

Objectives: The objective of this study is to identify toxicants causing acute toxicity in effluents from the aluminum rolling industry that violate the discharge limits in Korea. Methods: Whole effluent toxicity tests (WET) were conducted on effluent discharged from the aluminum rolling industry following the US EPA WET test methods. We collected effluent samples three times and evaluated acute toxicity by using Daphnia magna. We employed toxicity identification evaluation (TIE) procedures to identify toxicants causing toxicity in the effluent. Results: No specific chemical groups were identified in the seven different manipulations applied to the of wastewater effluent samples showing 1.3 toxic units (TU) according to the TIE phase I procedures. Water quality parameters for water hardness, electric conductivity and heavy metals (Mn) were 4,322 mg/l as $CaCO_3$, 11.39 mS/cm, and $5,551{\mu}g/l$, respectively. Considering water hardness and reference toxicity, high concentrations of Mn can be disqualified from the causative toxicants. Consequently, high ionic concentrations of $Na^+$(1,648 mg/l), $Ca^{2+}$(1,048 mg/l), $Mg^{2+}$(1,428 mg/l) and $SO_4{^{2-}}$(7,472 mg/l) were identified to be causative toxicants. Water hardness and electric conductivity exceed the $EC_{50}$ value obtained by biological toxicity tests using Daphnia magna. Conclusion: According to TIE procedures, high salt concentration is determined to be a major toxicant in the effluent of agro-industrial wastewater treatment plants receiving wastewater from the aluminum rolling industry.

• 분석과학
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• 제30권2호
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• pp.102-111
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• 2017

Here we reported a methodology for identification of triacylglycerols (TAGs) and diacylglycerols (DAGs) in coix seed by preparative thin layer chromatography (prep-TLC) and non-aqueous reversed-phase liquid chromatography (NARP LC)-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (MS/MS). Lipid components were extracted from coix seed by reflux extraction using n-hexane for 3 hr. TAGs and DAGs in coix seed extract were effectively purified and isolated from matrix interferences by prep-TLC and then analyzed by LC-APCI-MS and MS/MS for identification. TAGs were effectively identified taking into consideration of their LC retention behavior, APCI-MS spectra patterns, and MS/MS spectra of $[DAG]^+$ ions. In MS/MS spectra of TAGs, diacylglycerol-like fragment $[DAG]^+$ ions were useful to identify TAGs with isobaric fragment ions. Based on an established method, 27 TAGs and 8 DAGs were identified in coix seed extract. Among them, 15 TAGs and 8 DAGs were for the first time observed in coix seed. Interestingly, some of TAGs isolated by prep-TLC were partly converted into DAGs through probably photolysis process during storing in room temperature. Thus, degradation phenomenon of TAGs should be considered in the quality evaluation and nutritional property of coix seed. LC-APCI-MS/MS combined with prep-TLC will be practical method for precise TAG and DAG analysis of other herbal plants.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2008년도 정기학술발표대회 논문집
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• pp.487-490
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• 2008

최근 활발히 진행되고 있는 도시재생사업과 같은 대규모 복합개발사업은 사업기간이 길고 다양한 사업주체들이 참여한다. 이러한 특성 때문에 사업추진 과정상 많은 위험요인들을 내포하게 되고 이를 관리하는 것은 사업의 성패에 큰 영향을 미친다. 그러므로 도시재생사업에서 위험을 인지하고 대응하는 일련의 위험관리 과정은 정확하고 체계적으로 수행되어야 한다. 위험관리과정에서 위험인지단계는 다양한 위험요인을 인지하고 그 위험의 성격을 규정하는 첫 단계이다. 위험을 인지하는 기법 중 체크리스트는 가장 보편적이고 실용적인 방법이라고 할 수 있다. 따라서 본 연구에서는 도시재생사업의 개발단계에서 사업주체들의 업무를 분석하고, 위험을 도출하여 사업주체별로 위험을 분류하는 체크리스트를 제안하였다. 이러한 주체별 위험요인 체크리스트는 실무자가 쉽게 위험을 파악할 수 있는 도구로써, 도시재생사업과 같이 다양한 사업주체들이 자신은 물론 다른 사업 참여자들의 위험을 인지하는데도 효과적일 것이라고 기대된다.

• 대한간호학회지
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• 제28권2호
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• pp.403-413
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• 1998

The Resilience is described as the personal capacity which brings psychosocial comeback. The role of nursing is to do its best to rehabilitate patients and to explore the individual in order to promote patients psychosocial change. However, as the current nursing is heavily physical nursing oriented, the identity of the nursing would be lost. Therefore this researcher reviewed if the concept of resilience can be applied to the nursing after examing the concept of resilience by Documents and Fieldwork. The methodology of this research is Hybrid Model developed by Schwartz-Barcott and Kim for the concept development and analysis. The process and procedure consist of The Theoretical Phase, The Fieldwork Phase and The Final Analytical Phase in accodance with the Hybrid Model. The followings the summary of the Research. 1. The Concept of Resilience Finally Analyzed by Documents and Fieldwork (1) The Redefinition of Resilience The resilience is the latent psychosocial capacity which minimize the negative emotion and promote the adaptation under adversity. Resilience appears as cognitive, emotional and behavioral response in the course of changing from negative response to positive response through the interaction of the individual and the enviroments in a given time. Resilience changes and decreases according to time and situation and it can be nurtured. Resilience is the higher concept including hardiness, sense of coherence and self-strength which maintain the health under stress. (2) The Attribute of Resilience The attribute of resilience was devided into psychological and social dimension. In psychological attributes, there are admittion of reality of situation, denial of negative emotion, desire to live, responsibility, confidence, courage, hope, pursuit of positive meaning, identification and pursuit of goal, self-esteem, reception, spontaneity, planning, positiveness, will power, flexibility and creativity. In social attributes, there are a sense of belonging, perception of social support and active social relations. (3) The Process of Resilience There are 4 resilience phases which were the process minimizing the possibility of the negative chain reactions under adversity, the process minimizing the negative emotion under adversity, the process gaining the desire to live and the process exposing the active social relations. 2. The Application Possibility of Resilience Concept to Nursing The resilience concept is the psychosocial capacity with which an individual manages adversity. As many nursing scientists have developed nursing theory based on this capacity and the identification of nursing has been established in this field, resilience is not the new conception in nursing. However, since resilience appears in the attributes related with the resilience process concretely, it would help a lot when nurses execute psychosocial nursing.

• 한국비블리아학회지
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• 제35권2호
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• pp.5-23
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• 2024

온라인 자료의 납본 시행 7년차를 맞이하여 체계적인 수집을 위해 동향조사, 법·제도 개선, 온라인 자료 중장기 수집 전략계획 마련이 요구되고 있다. 이에 본 연구에서는 온라인 자원을 국가 지식문화유산으로 수집·보존하여 후대에 전승하기 위한 온라인 자료 수집 전략 및 중장기 로드맵을 수립해서 제안하고자 하였다. 이를 위해 국내외 도서관의 현황, 관련 법·제도, 온라인 자료의 유형 및 수집현황을 분석하고, 이에 기반하여 실질적인 수집 기준과 방안을 제시하였다. 최종적으로 중장기 로드맵 및 실행 계획을 제안하였다. 온라인 자료 수집과 관련한 중장기 발전계획을 수립하여, 단계별로 구체적인 실행 계획을 제안하였다. 이는 온라인 자료 수집의 기반 조성부터 시작하여 확산 단계, 그리고 최종적으로는 성숙 단계에 이르는 과정을 포함한다.

• 한국소음진동공학회논문집
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• 제20권5호
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• pp.486-491
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• 2010

In this paper, it is identified that high-level vibration of the single phase main power transformers shut down due to the mechanical fault. vibration sources of the SPR in the transformer's are identified and the SPR vibrations are reduced by structural modification. For vibration characteristic identification, vibration signals were measured with an accelerometer when the transformer is driving. These signals are presented in a FFT analysis in order to find the dependency of frequency components on the transformer. From finite element analyses and some experiments, it is also found that resonances occur because the natural frequencies of the SPR exist in usual driving range. To shift the natural frequencies outside the driving range, the SPR is modified by increasing stiffness. It is verified that considerable amounts of vibration are reduced by the structural modification.

• 대한기계학회논문집B
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• 제24권2호
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• pp.306-315
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• 2000

An experimental study was conducted to examine the internal flow patterns inside the mixing chamber of effervescent atomizers. The mixing chamber has the rectangular cross section ($8mm{\times}2mm$) and made of transparent acrylic plate for flow visualization. The parameters tested were the air/liquid ratio (ALR), injection. pressure, and the nozzle orifice diameter. Three different flow regimes were observed; bubbly, annular, and intermittent flows. In the bubbly flow regime, the discharged mixture was disintegrated into drops through the bubble expansion and the ligament breakup. On the other hand, in the annular flow regime, the liquid annulus was disintegrated into small drops by the aerodynamic interaction between the phases due to the high relative velocities between the gas and the liquid. In the intermittent flow regime, the bubble-expansion/ligament-disintegration mode and the annulus-disintegration mode appeared alternatively. The correlations representing the transition criteria between the two-phase flow patterns within the mixing chamber were proposed based on the drift-flux models.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2004년도 Annual Meeting and International Symposium
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• pp.17-23
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• 2004

Not all individuals respond identically, or at times in the same direction, to dietary interventions. These inconsistencies likely arise because of diet and genomic interactions (nutrigenomics effects). A host of factors may influence the response to bioactive food components including specific polymorphisms (nutrigenetic effect), DNA methylation patterns and other epigenomic factors (nutritional epigenomic effects), capacity to induce anuo. suppress specific mRNA expression and patterns (nutritional transcriptomics), the occurrence and activity of proteins (proteomic effects), and/or the dose and temporal changes in cellular small molecular weight compounds will not only provide clues about specificity in response to food components, but assist in the identification of surrogate tissues and biomarkers that can predict a response. While this 'discovery' phase is critical for defining mechanisms and targets, and thus those who will benefit most from intervention, its true usefulness depends on moving this understanding into 'development' (interventions for better prevention, detection, diagnosis, and treatment) and a 'delivery' phase where information is provided to those most in need. It is incumbent on those involved with food and nutrition to embrace the 'omics' that relate to nutrition when considering not only the nutritional value of foods and their food components, but also when addressing acceptability and safety. The future of 'Nutrigenomics and Health Promotion' depends on the ability of the scientific community to identity appropriate biomarkers and susceptibility variants, effective communications about the merits of such undertakings with the health care community and with consumers, and doing all of this within a responsible bioethical framework.