• Title/Summary/Keyword: Phase and Magnitude

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Transformation of Bacillus stearothermophilus No. 236 by Changing Incubation Temperature after Electroporation

  • Ha, Gyong-Sik;Kim, Joon;Choi, Yong-Jin
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.687-690
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    • 1999
  • Bacillus stearothermophilus No. 236 isolated from the soil is a strong xylan degrader producing all the xylanolytic enzymes. However, the strain was discovered to be highly intractable to its transformation. In the present study, we have developed a reliable method for transformation of B. stearothermophilus No. 236 by a systematic examination of several factors which might have an influence on the efficiency of electrotransformation. Notably, we found that the most critical factor influencing the transformation efficiency (TE) was the incubation temperature after pulsing, with its optimum incubation of $37^{\circ}C.\; At\; 50^{\circ}C$, the optimum growth temperature of the B. stearothermophilus strain, the transformants could not be obtained at a recognizable level. The combination of field strength of 7.5 kV/cm along with pulse duration of 10 msec (resistance of $400{\Omega}\; and\; capacitance\; of\; 25{\mu}F$) was shown to be the best electrical parameters at the incubation temperature of $37^{\circ}$. A higher TE was obtained when the cells were harvested at an early-exponential phase. Twenty percent of PEG-8000 in a suspension buffer and an addition of 0.1% glycine in the growth medium resulted in about 4-fold and 3-fold increases in TE, respectively. We also found that the plasmid DNA which had been cycled through the host B. stearothermophilus cells enhanced TE by one order of magnitude higher. Under the presently described conditions, $2.5{\times}10^{5} transformants per ${\mu}g$ DNA was attained.

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Performance Analysis of the Uni-Directional Radiation Equiangular Antenna over EBG Surface (EBG 표면 위의 단일 방향 복사 등각 안테나의 성능 해석)

  • Yoon, Sung Hyun;Kim, Jae Kwan
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.8
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    • pp.1622-1630
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    • 2015
  • In this study, we used EBG(electromagnetic band gap) reflector to change bi-directional radiation of circular polarization into uni-directional radiation of equiangular spiral antenna. When the height of spiral arm from EBG reflector is 0.07 wavelength of the lowest operating frequency, the axial ratio of the circular polarization was deteriorated. In this paper, we analyzed the magnitude and the time phase difference of $E_{\theta},E_{\phi}$ that generates right hand circle polarization that is co-polarization at +z direction and proposed the improving condition for axial ratio at all related frequency range. As a result, we obtained that the axial ratio was below 3[dB] at range of 3 ~ 10[dB], the gain was improved about 3[dB] with comparison to bi-directional radiation at free space, and $S_{11}$ was below -10[dB] at all related frequency range.

The Studies on Qigong state Using EEG, fMRI, EAV and SQUID Measurments (EEG, fMRI, EAV 및 SQUID장치(裝置)를 이용(利用)한 기공현상(氣功現狀) 측정(測定))

  • Jeong, Chan-Won;Choi, Chan-Hun;Yoon, Wu-Sik;So, Cheal-Ho;Na, Chang-Su;Jang, Kyeong-Seon
    • Korean Journal of Acupuncture
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    • v.21 no.2
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    • pp.1-28
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    • 2004
  • Objectives : Human physiological changes in the state of qigong has been measured using EEG(Electroencephalography), functional MRI(functional Magnetic Resonance Image), EAV(Electro-Acupuncture according to Voll) and SQUID(Superconducting Quantum Interference Device) measurements. Methods & Results : EEGs were measured to study the differences between Qigong masters and Qi receiver on the changes of EEG. During Qigong, an alpha waves were increased. The power spectra indicate that the peak frequency of alpha waves increased during Qigong. Qi receiver's EEG signals seemed to affected by the state of himself. Brain activation did not observed when qigong master concentrates the Qi at Laogong(P8). But a localization of fMRI signal in the sensory cortex was observed by electric acupuncture stimulation at Laogong(P8). Five phase deviation of EAV were clearly changed in the both cases of Qigong master and Qi receiver. When a Qigong master concentrates the Qi at Yintang, Laogong(P8), Qihai(CV6) meridian points during Qigong state, the change of magnetic field around acupoints Yintang, Laogong points has been measured using 40-Channel DROS-SQUID apparatus. After smoothing process of the continuously measured magnetic signal around acupoints for a few minutes, we could observe that a series of peaks, magnitude of -1.0~2.5pT appeared. But there was no significant difference in changes of magnetic signal around acupoints. Physical signals of magnetocardiogram has been measured by using 2-Channel DROS SQUID(Magnetocardiogram). Physical signals of magnetocardiogram were clealy changed at the ST segments after S-wave when qigong master concentrates the Qi.

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A Novel Method to Reduce Local Oscillator Leakage (국부발진기에서의 누설신호의 새로운 제거방식)

  • 이병제;강기조
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.2
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    • pp.294-301
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    • 2000
  • One of the most important design parameters in a microwave radio transmitting system is to reduce spurious response from the output spectrum of the transmitting system. A Local oscillator (LO) is seldom totally pure and at the least contain some LO harmonic signals. A LO or any oscillator is a transmitter if provided with a suitable radiator, conduction, or leakage path. Where mixer is employed in the output of the LO mixer generated spurs can be increased by RF amplifier. To reduce LO leakage, notch filter or band pass filter has been conventionally used. In this paper, the leakage reduction(LR) signal, which has the same magnitude and the opposite phase with respect to LO leakage signal, is added to the output of mixer of the wireless LAN system. The LO leakage is reduced by 30 dB more than the conventional methods do. The proposed method is potentially suitable for low-cost, reliable, and simple application of monolithic microwave integrated circuits (MMICs)

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Retrieval of Soil Moisture Using Microwave Reflection at the End of a Coaxial Probe (동축선 끝단에서의 마이크로파 반사를 이용한 토양 수분 함유량 산출 기술)

  • 김태진;오이석
    • Korean Journal of Remote Sensing
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    • v.13 no.2
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    • pp.151-163
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    • 1997
  • In this paper, an algorithm for retrieving soil moisture from measurement of microwave reflection at the end of a coaxial canble is presented. Because the wave reflection from the boundary between air and soil layers depends on the dielectric constant of the soil layer, the dielectric constant can be obtained from measured reflection coefficient. At first, an equivalent circuit for the coaxial probe contaced on the soil surface was chosen with two unknown circuit elements. Then, the unknown circuit elements are obtained experrmentally by measuring the reflection constants of 20 soil samples, and consequently, an empirical formula for computing the dielectric constant from the reflection coefficient is obtained. The dielectric constant is mainly influenced by the soil moisture, and the soil moisture can be computed from the dielecfic constant using an existing empirical formula. HP Network Analyzer 8510C was used to measure the magnitude and the phase of the reflection coeffcient at 4.65 GHz, and the measured data set were used to obtain an empirical formula for computing the dielectric constant. The empirical formula obtained in this study was proven by other soil samples.

Complex nested U-Net-based speech enhancement model using a dual-branch decoder (이중 분기 디코더를 사용하는 복소 중첩 U-Net 기반 음성 향상 모델)

  • Seorim Hwang;Sung Wook Park;Youngcheol Park
    • The Journal of the Acoustical Society of Korea
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    • v.43 no.2
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    • pp.253-259
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    • 2024
  • This paper proposes a new speech enhancement model based on a complex nested U-Net with a dual-branch decoder. The proposed model consists of a complex nested U-Net to simultaneously estimate the magnitude and phase components of the speech signal, and the decoder has a dual-branch decoder structure that performs spectral mapping and time-frequency masking in each branch. At this time, compared to the single-branch decoder structure, the dual-branch decoder structure allows noise to be effectively removed while minimizing the loss of speech information. The experiment was conducted on the VoiceBank + DEMAND database, commonly used for speech enhancement model training, and was evaluated through various objective evaluation metrics. As a result of the experiment, the complex nested U-Net-based speech enhancement model using a dual-branch decoder increased the Perceptual Evaluation of Speech Quality (PESQ) score by about 0.13 compared to the baseline, and showed a higher objective evaluation score than recently proposed speech enhancement models.

Thermotropic Liquid Crystalline Behaviors of 4-{4'-(nitrophenylazo)phenoxy}alkanoic Acids and 4-{4'-(nitrophenylazo)phenoxy}alkanoyl Chlorides (4-{4'-(니트로페닐아조)펜옥시}알칸 산들 그리고 4-{4'-(니트로페닐아조)펜옥시}알카노일 클로라이드들의 열방성 액정 거동)

  • Jeong, Seung Yong;Ma, Yung Dae
    • Applied Chemistry for Engineering
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    • v.19 no.5
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    • pp.504-511
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    • 2008
  • Two kinds of nitroazobenzene derivatives: 4-{4'-(nitrophenylazo)phenoxy}alkanoic acids (NAAn, n = 2~8, 10, number of methylene units in the alkyl chain) and 4-{4'-(nitrophenylazo)phenoxy}alkanoyl chlorides (NACn, n = 2~8, 10) were synthesized, and their thermotropic liquid crystalline behaviors were investigated. NAA6 formed an enantiotropic nematic phase, while the remainders, except NAA2, showed monotropic nematic phases. Isotropic-nematic transition temperature ($T_{iN}$) and change of entropy (${\Delta}S$) at $T_{iN}$ for both of NAAn and NACn varied by the change of n, and pronounced odd-even effects of n were also observed. However, the $T_{iN}$ and ${\Delta}S$ values of NAAn were much higher than those of NACn. This fact may be attributed to the hydrogen bonding between carboxyl groups. Thermal properties and degree of order in the mesophase and the magnitude of the odd-even effects of both NAAn and NACn were significantly different from those reported for 4-(alkoxy)-4'-nitroazobenzenes. It was discussed in terms of the differences in the molecular anisotropy and the temperature-dependent flexibility of the substituted groups.

The 33-mode Dielectric and Piezoelectric Properties of PIN-PMN-PT Single Crystal under Stress and Electric Field (압축하중 및 전계 인가에 따른 PIN-PMN-PT 단결정의 33-모드 유전 및 압전특성)

  • Lim, Jae Gwang;Park, Jae Hwan;Lee, Jeongho;Lee, Sang Goo
    • Journal of the Microelectronics and Packaging Society
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    • v.27 no.4
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    • pp.91-96
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    • 2020
  • The 33-mode dielectric and piezoelectric properties of Pb(In1/2Nb1/2)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 piezoelectric single crystals were measured under large electric field and compressive stress. The phase transition from the low temperature rhombohedral to the high temperature tetragonal structure was observed in the range of 110~140℃, and the Curie temperature changing to the cubic structure was about 165℃. The polarization change according to the compressive stress and electric field was measured. Relative dielectric constant was calculated from the slope of the polarization curve applied to the electric field, and the calculated relative dielectric constant increased as the applied stress increased, and the relative dielectric constant decreased as the applied electric field increased. The strain according to the compressive stress and electric field change was measured, the piezoelectric constant was calculated from the slope of the curve, and the phase transition according to the application of pressure was confirmed. In the case of practical application as an underwater or medical ultrasonic actuator, it is necessary to properly design the magnitude of the compressive stress applied to the device and the DC bias in order to maintain linear driving.

Design of 2nd-harmonic Quadrature Mixer for Ultra Wideband(UWB) Systems (2차 고조파를 이용한 UWB 시스템용 쿼드러쳐 혼합기 설계)

  • Jung, Goo-Young;Lim, Jong-Hyuk;Choi, Byung-Hyun;Yun, Tae-Yeoul
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.12 s.115
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    • pp.1156-1163
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    • 2006
  • This paper presents an ultra wideband(UWB) direct conversion mixer for IEEE 802.15.3a applications with simulation and measurement results. Since the direct conversion mixing causes dc-offset and even-order distortion, the proposed mixer adopts an anti-parallel diode pairs(APDPs) to solve these problems. The proposed mixer consists of an in-phase wilkinson power divider over $3.1{\sim}4.8GHz$, a wideband $45^{\circ}$ power divider over $1.5{\sim}2.4GHz$, and miniatured band pass filters(BPFs) for RF-LO isolations. The conversion loss is optimized with impedance matchings between APDPs and wideband components. The measured mixer shows the conversion loss of 13.5 dB, input third-order intercept-point($IIP_3$) of 7 dBm, and 1-dB gam compression point($P_{1dB}$) of -4 dBm. Quadrature(I/Q) outputs have the magnitude difference of about 1 dB and phase difference of ${\pm}3^{\circ}$.

The Gain and Phase Mismatch Detection Method with Closed Form Solution for LINC System Implementation (LINC 시스템 구현을 위한 닫힌 해를 갖는 크기 위상 오차 검출 기법)

  • Myoung, Seong-Sik;Lee, Il-Kyoo;Lim, Kyu-Tae;Yook, Jong-Gwan;Laskar, Joy
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.5
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    • pp.547-555
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    • 2008
  • This parer proposed the path mismatch detection and compensation algorithm with closed form for linear amplification with non-linear components(LINC) system implementation. The LINC system has a merit of using the high efficient amplifier by transferring the non-constant envelop signal which is high peak to average signal ratio into constant envelop signal. However, the performance degradation is very sensitive to the path mismatch such as an amplitude mismatch and a phase mismatch. In order to improve the path mismatch, the error detection and compensation method is introduced by the use of four test signals. Since the presented method has the closed form solution, the efficient and fast detection is available. The digital-IF structure of LINC system applied by the proposed error detection and compensation algorithm was implemented. The performance was evaluated with the IEEE 802.16 WiMAX baseband sinal which has 7 MHz channel bandwidth and 16-QAM. The Error Vector Magnitude(EVM) of -37.37 dB was obtained through performance test, which meets performance requirement of -24 dB EVM. As a result, the introduced error detection and compensation method was verified to improve the LINC system performance.