• Title/Summary/Keyword: Pharmaceutics

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A novel pattern recognition protein of the Chinese oak silkmoth, Antheraea pernyi, is involved in the pro-PO activating system

  • Wang, Xialu;Zhang, Jinghai;Chen, Ying;Ma, Youlei;Zou, Wenjun;Ding, Guoyuan;Li, Wei;Zhao, Mingyi;Wu, Chunfu;Zhang, Rong
    • BMB Reports
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    • v.46 no.7
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    • pp.358-363
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    • 2013

  • In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.

  • https://doi.org/10.5483/BMBRep.2013.46.7.009 인용 PDF KSCI KPUBS HTML

Effect of Sodium Taurodihydrofusidate on Nasal Drug Delivery: Differences in Its Concentration and Penetrant Molecular Weight

  • Hosoya, Ken-ichi;Kubo, Hiroyuki;Takashi-Akutsu;Hideshi-Natsume;Kenji-Sugibayashi;Yasunori-Morimoto
    • Archives of Pharmacal Research
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    • v.17 no.2
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    • pp.57-59
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    • 1994

  • The effect of sodium taurodihydrofusidate (STDHF) on drug permeation across nasal mucosa was studied in vitro using Ussing type diffusion chamber. Disodium cromoglicate (DSCG, M.W.5123) and fluorescein isothiocyanate-dextran (FD) of different molecular wieghts (M.W. 4400-71200) were used as model drugs. Pemeation profiles of DSCG and FDs showed a typical pseudo steady-state curve with short lag time. The pemeability coefficient of FD (M.W. 9400) sigmodially increased with increasing STDHF concentration. It also enhanced the DSCG pemeation. Interestingly the enhancement efficacy was independent of molecular weight of penetrants.

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