• Korean Chemical Engineering Research
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• 제51권5호
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• pp.536-539
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• 2013

본 연구에서는 황벽나무, 두충나무 등을 포함하는 복합수목추출물의 항균활성 효과 및 안전성에 대해 검토하였다. 항균활성은 피부상재균이며 기회 병원성균인 Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli 및 Candida albicans에 대해 disc 확산법으로 실험되었다. 안전성 시험으로는 단회 경구투여 독성시험, 단회투여 흡입독성 시험, 반복투여 흡입독성시험이 실시되었다. 항균력효과시험 결과 추출물은 Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans에 대해 우수한 항균활성을 보여주었으나, Escherichia coli에서는 항균활성이 없었다. 복합수목추출물의 단회 경구투여 독성시험, 단회투여 흡입독성시험 및 반복투여 흡입독성시험의 결과 독성은 관찰되지 않았다. 따라서 황벽나무, 두충나무 등을 포함한 복합수목추출물은 천연 항살균제로서 상업화 가능성이 매우 높음을 알 수 있었다.

• 분석과학
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• 제34권2호
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• pp.37-45
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• 2021

A rapid and simple LC-MS/MS analytical method in determining Jaspine B has been developed and validated in rat plasma. The standard curve value was 25 - 5000 ng/mL and the linearity, inter-day and intra-day accuracy and precision were within 15.0 % of relative standard deviation (RSD). The mean recoveries of Jaspine B ranged from 87.5 % to 91.2 % with less than 3.70 % RSD and the matrix effects ranged from 91.1 % to 108.2 % with less than 2.6 % RSD. The validated LC-MS/MS analytical method of Jaspine B was successfully applied to investigate the dose-escalated pharmacokinetic study of Jaspine B in rats following an intravenous injection of Jaspine B at a dose range of 1 - 10 mg/kg. The initial plasma concentrations and area under plasma concentration curves showed a good correlation with intravenous Jaspine B dose, indicating the dose independent pharmacokinetics of Jaspine B in rats. In conclusion, this analytical method for Jaspine B can be easily applied in the bioanalysis and pharmacokinetic studies of Jaspine B, including its administration at multiple therapeutic doses, or for making pharmacokinetic comparisons for the oral formulations of Jaspine B in small experimental animals as well as in vivo pharmacokinetic-pharmacodynamic correlation studies.

• Natural Product Sciences
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• 제13권1호
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• pp.54-60
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• 2007

The plant Ichnocarpus frutescens (Linn) R.Br. (Family-Apocynaceae) has been indicated for the treatment of various diseases, one amongst it is cancer. The purpose of this study was to investigate experimentally the possible antitumor activity and antioxidant role of Ichnocarpus frutescens in the mice transplanted with Ehrlich ascites carcinoma (EAC). The chloroform and methanol extract of whole plant of Ichnocarpus frutescens (CEIF and MEIF) were administered intraperitoneally at the dose of 150 mg/kg and 300 mg/kg, body weight per day for 7 days after 24 h of tumor inoculation in mice. Treatment with CEIF at the dose of 150 mg/kg and 300 mg/kg remarkably decreased the tumor volume, packed cell volume, viable cell count and increased the nonviable cell count of EAC tumor bearing mice when compared to e effect of MEIF at 150 mg/kg and 300 mg/kg. Further the EAC mice treated with CEIF and MEIF showed significant decrease in the level of lipid peroxidation and significant increase in the level of antioxidant enzymes such as glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT), however the decreasing and increasing capacity of CEIF was less in both doses as compared to MEIF. Based on these results, it can be concluded that the chloroform and methanol extact of Ichnocarpus frutescens exhibit significant antitumor and antioxidant activity in EAC bearing mice.

• Biomolecules & Therapeutics
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• 제6권1호
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• pp.56-62
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• 1998

Mutagenicity of recombinant human erythropoietin (rhEPO) was examined in the reverse mutation test on bacteria, in the chromosomal aberration test on cultured mammalian cells and in the micronucleus test on mice. The reverse mutation test was performed by a plate incorporation method with or wothout a metabolic activation system (59 Mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA 1537. The rhEPO did not significantly increase revertant colonies in any of the test strains under any conditions at dose levels ranging from 1000 H/ml to 62.5 lu/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese Hamster Lung (CHL) cells, the number of aberrant cells was not increased in the presence or absence of 59 Mix at concentrations of 1000 lU/ml to 250 lU/ml, compared with the vehicle control. In the micronucleus test, male ICR mice were given rhEPO intraperitoneally at a dose level of 25000, 12500 and 6250 lU/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes was not different from that of the vehicle control. From these results, rhEPO is considered to be non-mutagenic under the present test conditions.

• Advances in Traditional Medicine
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• 제8권3호
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• pp.311-315
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• 2008

The objective of the study was to investigate the antihyperlipidemic activity of alcoholic extract of Pongamia pinnata Linn. (PPAE) leaves in rats fed with high fat diet (HFD). PPAE was administered orally in the divided doses of 250 and 500 mg/kg/day for 30 days in HFD fed rats. Body weights were observed and the analysis of serum lipid profile was carried out on day 30. Marked decrease in the body weight, total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) whereas significant increase in the levels of high density lipoprotein (HDL) were observed after treatment with PPAE. However, PPAE in a dose of 250 mg/kg did not show significant (P < 0.05) increase in HDL levels. The PPAE also lowered TC: HDL-c and LDL: HDL-c ratios significantly suggesting it's antihyperlipidemic and cardioprotective potential. The present work reveals that PPAE at the dose of 500 mg/kg/day exhibited significant (P < 0.01) antihyperlipidemic effects.

• Advances in Traditional Medicine
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• 제7권2호
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• pp.133-140
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• 2007

Anti-tumor activity of Euphorbia hirta (50 mg/kg and 100 mg/kg) has been evaluated against Ehrlich's ascites carcinoma (EAC) in Swiss albino mice. Intraperitoneal (i.p) administration of Euphorbia hirta was effective in reducing solid tumor mass development induced by EAC cells. It exhibited significant anti-tumor activity in mice, when used at the dose of 100 mg/kg/day i.p., for 14days. The administration of Euphorbia hirta (100 mg/kg/day i.p.) resulted in an increase (P<0.001) of the life span (59.9%) of ascites tumor bearing mice as compared to the control group. After 14 days, on developed tumor masses, Euphorbia hirta administration brought about significant reduction in tumor volume and it reverse the changes in the hematological parameters, responding to tumor inoculation. The results are indicative of the anti-tumor activity of Euphorbia hirta against EAC induced tumor in a dose dependent manner.

• Natural Product Sciences
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• 제4권4호
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• pp.226-229
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• 1998

Methanolic extract of Cassia tora leaves was evaluated for its hepatoprotective activity in rats by inducing hepatotoxicity with paracetamol (acute model). The extract at a dose of 400 mg/kg orally exhibited significant protective effect by lowering the serum levels of transaminase (SGOT and SGPT), bilirubin, and alkaline phosphatase (ALP). The effects produced were comparable to that of a standard hepatoprotective agent.

• Korean Chemical Engineering Research
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• 제54권6호
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• pp.762-766
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• 2016

본 연구에서는 황벽나무, 두충나무, 귀룽나무 추출물 등을 포함하는 천연추출물에대해 항균활성 효과 및 안전성에 대해 검토하였다. 항균활성은 기회 병원성균인 Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli 및 Candida albicans에 대해 실험되었다. 안전성 시험으로는 세포독성실험, 단회 경구투여 독성시험, 단회 흡입투여 독성시험, 반복 흡입투여 독성시험, 안점막 자극시험이 실시되었다. 항균력효과시험 결과 추출물은 Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans 4가지 균 모두에 대해 우수한 항균활성을 보여주었다. 천연추출물의 단회 경구투여 독성시험, 단회 흡입투여 독성시험 및 반복 흡입투여 독성시험, 안점막 자극시험의 결과 독성은 관찰되지 않았다. 따라서 황벽나무, 두충나무, 귀룽나무 추출물 등을 포함한 천연추출물은 뛰어난 안전성과 항균력이 있음을 확인하였다.

• Biomolecules & Therapeutics
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• 제7권1호
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• pp.7-13
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• 1999

The stimulatory effect of recombinant human basic fibroblast growth factor (bFGF) on wound healing was evaluated in healing-impaired animal models. Full-thickness wounds were made in prednisolone-treated mice, streptozotocin (STZ)-induced diabetic rats and mitomycin C (MMC)-treated rats. Saline or bFGF at a dose of 1, 5, or $25\mu\textrm{g}$ per wound was applied to the open wound once a day for three to five days. The degree of wound healing was assessed using wound size and histological parameters such as degree of epidermal and dermal regeneration. Local application of bFGF accelerated wound closure significantly in a dose-dependent manner in all healing-impaired wounds (p<0.05). The wound healing effect of bFGF was further confirmed by histological examination in MMC-treated rats. Epidermal and dermal regeneration were enhanced in bFGF-treated wounds with a dose-related response. Dermal regeneration parameters such as collagen matrix formation and angiogenesis were significantly increased in $5\mu\textrm{g}$, or $\25mu\textrm{g}$ of bFGF-treated wounds when compared to saline-treated wounds (p<0.05). pectin immunostaining on day 8 for vascular endothelium showed an increased number of neovessels in bFGF-treated wounds. These results suggest that topical application of bFGF has beneficial effects on wound healing by angiogenesis and granulation tissue formation in healing-impaired wounds.

• Journal of Pharmaceutical Investigation
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• 제30권1호
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• pp.39-45
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• 2000

We have investigated the efficacy of liposome encapsulated N-(phosphonacetyl)-L-aspartic acid (PALA) for the treatment of the C-26 murine colon tumor in Balb/c mice, and have compared it in this regard to free PALA. Healthy female Balb/c mice and C-26 tumor inoculated mice were randomized for the maximum tolerated dose (MTD) study and the in vivo therapy study, and the survival was measured after a single intraperitoneal injection of the drug. The maximum tolerated dose for intraperitoneally administered drug was found to be 750 mg/Kg for free PALA, and was greater than the maximum dose possible (150 mg/Kg) for PALA encapsulated in both DSPC and DSPG liposomes. When drug was administered one day after tumor implantation, 150 mg/Kg of PALA in DSPG liposomes increased the percentage of tumor bearing mice surviving at day 36 from 8% (buffer control) to 88%. In contrast, 150 mg/Kg free PALA increased the day 36 surviving percentage to only 25%. A 150 mg/Kg dose of PALA in DSPC liposomes increased the surviving percentage to 50%, while a 75 mg/Kg dose of PALA in sterically stabilized liposomes increased the surviving percentage to 78%. These results show that PALA in negatively charged or sterically stabilized liposomes can exhibit considerably greater potency than free PALA in C-26 tumor bearing mice.