• Title/Summary/Keyword: Pharmaceutical biotechnology

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Biotransformation of Rosamicin Antibiotic into 10,11-Dihydrorosamicin with Enhanced In Vitro Antibacterial Activity Against MRSA

  • Nguyen, Lan Huong;Nguyen, Huu Hoang;Shrestha, Anil;Sohng, Jae Kyung;Yoon, Yeo Joon;Park, Je Won
    • Journal of Microbiology and Biotechnology
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    • v.24 no.1
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    • pp.44-47
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    • 2014

  • A biotransformation approach using microbes as biocatalysts can be an efficient tool for the targeted modification of existing antibiotic chemical scaffolds to create previously uncharacterized therapeutic agents. By employing a recombinant Streptomyces venezuelae strain as a microbial catalyst, a reduced macrolide, 10,11-dihydrorosamicin, was created from rosamicin macrolide. Its chemical structure was spectroscopically elucidated, and the new rosamicin analog showed 2-4-fold higher antibacterial activity against two strains of methicillin-resistant Staphylococcus aureus compared with its parent rosamicin. This kind of biocatalytic approach is able to expand existing antibiotic entities and can also provide more diverse therapeutic resources.

  • https://doi.org/10.4014/jmb.1306.06054 인용 PDF KSCI KPUBS

An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos

  • Sun, Qiao;Wei, Wei;Zhao, Juan;Song, Jia;Peng, Fang;Zhang, Shaopeng;Zheng, Yonglian;Chen, Ping;Zhu, Wenjun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1528-1531
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    • 2015

  • Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.

  • https://doi.org/10.4014/jmb.1501.01053 인용 PDF KSCI KPUBS