• Journal of the Korean Institute of Landscape Architecture
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• v.33 no.5 s.112
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• pp.57-68
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• 2005

This study surveyed planting areas along streets in Gangwon-do to find out how to improve the planting and use of street trees and herbaceous plants. There were 301,491 trees of 41 species on the streets of Gangwon-do in 2004. The predominant species of street trees were Ginkgo biloba ($40\%$), Prunus spp. (Prunus yedoensis and Prunus sargentii) ($25\%$), Platanus occidentalis ($5\%$), followed by Betula platyphylla var. japonica, Zelkova serrata, Prunus armeniaca var. ansu, Acer palmatum, and Pinus thunbergii. Eighty-four herbaceous plant species were found in the Youngseo district (the southern area of Gangwon-do); the ratio of native species to exotic was 51:33. The predominant species were Cosmos bipinnatus, Petunia hybrida, Tagetes spp., Aster koraiensis, and Fagopyrum esculentum. Eighty-nine herbaceous plant species were found in the Youngdong district (the eastern area of Gangwon-do); the ratio of native species to exotic was 55:33. The predominant herbaceous plants were Aster koraiensis, Tagetes spp., Petunia hybrida, Rudbeckia bicolor, Cosmos bipinnatus, Salvia splendens, Brassica oleraceae var. acephala, Aquilegia buergeriana var. oxysepala, Coreopsis drummondii, Viola tricolor, and Dianthus superbus var. longicalycinus. Appropriate pruning adds to the aesthetic value of trees and prolongs their useful life; it also maintains good health and thereby reduces the need to control insects and diseases. Street trees had not been properly pruned due to the presence of power lines and a shortage of pruning information. The pruning was controlled by Korea Electric Power Company, which has no pruning information. Pruning must be maintained by a professional landscape company in order to maintain good shape, such as that which is done for bonsai. In order to improve the planting, use and maintenance of landscape plants in Gangwon-do, the following recommendations are made: street tree species should be diversified, suitable street trees should be selected for each space, native species should generally be used, trees should be appropriately pruned and properly fertilized, pests and diseases should be controlled, plantings should be done in multiple layers, spatial arrangements should be improved, larger trees should be planted, and drainage and underground electric wires should be considered when planting.

• Horticultural Science & Technology
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• v.18 no.1
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• pp.33-38
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• 2000

This study was conducted in an effort to meet the need for domestic production of hydroponic media and for medium development using recycled hydroponic rockwool slabs. Two experiments were conducted to evaluate the growth of plug seedlings of petunia 'Romeo' in various mixtures including particles of used rockwool slabs (rockwool particles) and wood chips. In the first experiment, seedlings were grown in plugs filled with mixtures of steam- sterilized rockwool particles and chestnut wood chips, which had been weathered for six months and screened through either 2.8 mm or 5.6 mm sieve, at the mixing ratio of 100:0, 75:25, 50:50, 25:75, or 0:100 (v/v). In the second experiment, seedlings were grown in plugs filled with twenty different mixtures of steam-sterilized particles of used hydroponic rockwool slabs with 4 mm chestnut or pine wood chips, coir, peatmoss, or perlite. In the first experiment seedling growth was not affected by particle size of chestnut wood chips, was superior in the control (commercial plug medium) and in 100% rockwool particles, but was suppressed as mixing ratio of chestnut wood chips increased. In the second experiment, leaf length, and shoot fresh and dry weights were the greatest in rockwool particles+peatmoss+coir treatment, followed by rockwool particles+peatmoss treatment. Leaf length was suppressed as ratio of wood chips increased, especially of chestnut wood chips. Root development was the greatest in rockwool particles+peatmoss+perlite treatment, followed by the control, and rockwool particles+peatmoss+coir treatment. The growth was suppressed as the mixing ratio of rockwool particles, peatmoss, or coir decreased.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.179-184
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• 2009

The correlation and combining abilities related with plant spreading characteristics of plant height, plant width, stem length, internode length, number of stem, number of leaves, and number of flower were studied in the $F_1s$ of 10 crosses from the partial five-parent diallel cross in Petunia hybrida. The plant width showed highly positive correlation with internode length, and number of flower. The mean squares of general combining ability(GCA) and specific combining ability(SCA) were highly significant for plant width, number of stem, number of leaves, and number of flowers. The mean square values of GCA were greater than those of SCA for all the characters, showing preponderance of additive gene actions for these characters. The lines of D and I for plant width and length of stem showed relatively high GCA effects. The crosses of $D{\times}I$, $F{\times}I$, and $G{\times}I$ exhibited high SCA effects on plant width, especially $G{\times}I$ effected in internode length and number of flower. The broad sense heritability was generally high compared to narrow sense one. Plant width and stem length, and internode length related to spreading growth showed the higher heritability than the other characters in the broad and narrow sense.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.140.1-140
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• 2003

An Isometric virus was isolated from Paprika (Capsicum annuum var. glossum) showing necrosis spot and malformation on the fruit and the leaves, respectively, at yecheon in Korea. The virus could infect locally on Chenopodium amaranticolr, C. quinoa, Petunia x hybrida and Nicotiana glutinosa, but could not infect on Gomphrena globosa and Physalis floridana. The virus could infect systemically on red pepper and Lycopersicon esculentum. Datura stramonium, N. cleuarandii, N. rustim and N. tabacum cvs. were produced necrosis or necrotic ring spot lesions on the inoculated leaves and mosaic, vein necrosis or lethal death on the upper leaves. The virus was not related serologically to cucumber mosaic virus (CMV). In RT-PCR assay, it could not detected with specific primers of CMV and BBWV-II. The virions contain one molecule of genomic RNA, Which was approximately 3.8Kb and the coat protein (CP) of the purified virion migrated as a single band with molecular wight of about 29KDa in SDS-PAGE.

• KSBB Journal
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• v.16 no.1
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• pp.36-40
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• 2001

In order to study the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in plants, we cloned and sequenced a partial glutamate decarboxylase (GAD) cDNA from the Arabidopsis thaliana cDNA library, using primers targeted at highly conserved sequences of the petunia GAD gene. The cDNA fragment was inserted into TA cloning vector with T7 promoter and the recombinant plasmid obtained was used to transform E. coli. The plasmid DNA purified from the transformed E. coli was digested with EcoRI and the presence of the insert was confirmed. Nucleotide sequence analysis showed that the fragment is a partial Arabidopsis thaliana GAD gene and that the sequence showed 98% and 78% identity to the region of the putative Arabidopsis thaliana GAD sequences deposited in GenBank, Accession nos: U46665 and U10034, respectively. The amino acid sequence deduced from the partial Arabidopsis thaliana GAD gene showed 99% and 91% identities to the GAD sequences deduced from the genes of the U46665 and U10034, respectively. The partial cDNA sequence determined may facilitate the study of the molecular mechanism of GABA metabolism in plants.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.297-301
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• 2004

A Tomato spotted wilt virus (TSWV-KP) was isolated from Paprika (Capsicum annuum var. grossum) showing necrosis spot on the leaves and malformation of the fruit in Yesan, Korea. The virus infected Chenopodium amaranticolor, C. quinoa, Petunia hybrida, Nicotiana glutunosa, Gomphrena globosa, and Physalis floridana. Ten plants including tomato were observed to have systemic TWSV-KP infection. The virus produced necrosis or necrotic ring spots on the inoculated leaves and mosaic, vein necrosis or death on the upper leaves of Datura stramonium, N. clevarandii, N. rustica, and N.tabacum cvs. Thin sections of the infected leaf tissue contained spherical to oval particles, a characteristic of a Tospovirus. The virion contained three molecules of genomic RNAs, which were approximately 9.0, 4.9 and 3.0 kb. The nucleocapsid (N) protein of the purified virion migrated as a single band with molecular weight of about 29 kDa in SDS-PAGE. The N gene of TSWV-KP showed 96.5-97.2% and 97.7-98.5% identities to the three different TSWV isolates of Genbank Database at the nucleotide and amino acid, respectively.

• Journal of Applied Biological Chemistry
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• v.42 no.1
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• pp.19-24
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• 1999

Plants have been shown to contains $Ca^{2+}$/calmodulin-stimulated GAD and NAD kinase. To test how calmodulin and calmodulin methylation affect the activation of GAD and NAD kinase, GAD and NAD kinase were partially purified from tobacco plants. GAD was also partially purified from E. coli transformed with a plasmid carrying a cloned tobacco GAD gene. We find that GAD from the transformed E. coli showed 60-fold $Ca^{2+}$/calmodulin-dependent activation. However, GAD from tobacco plants was stimulated only about 3.8-fold by the addition of calmodulin in the presence of calcium, suggesting high background activity of the enzyme was possibly due to bound endogenous tobacco calmodulin. There were no significant differences in the tobacco GAD activator properties between calmodulins. A monoclonal antibody against petunia GAD interacted strongly with both GAD from tobacco plants and GAD from cloned gene. NAD kinase from tobacco plants showed a complete $Ca^{2+}$/calmodulin dependency for activity. Unmethylated calmodulins activated GAD in a manner similar to methylated calmodulin. However, the maximum level of NAD kinase activation obtained with unmethylated calmodulins is approximately 4-fold higher than methylated calmodutins. These data suggested that endogenous tobacco calmodulin may interact more tightly with GAD than NAD kinase and that calmodulin methylation affects the activator properties of calmodulins for tobacco NAD kinase but not for GAD.

• Research in Plant Disease
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• v.20 no.3
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• pp.219-222
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• 2014

Several tomato production regions in Korea were surveyed for tomato yellow leaf curl disease (TYLCD). Tomato leaf samples showing TYLCD-like symptoms were collected from Tongyeong (To), Geoje (Gi), and Gimhae (Gh) cities of the southern part of Korea. Tomato yellow leaf curl virus (TYLCV) was detected and the full-length genomes of the isolates were sequenced. The TYLCV isolates found in Korea shared high sequence identity (> 99%) with TYLCV-IL [JR:Omu:Ng] (AB110217). Phylogenetic relationship analysis revealed that they formed two groups (with little genetic variability), and the To, Gj, and Gh isolates belonged to the TYLCV-IL group. An infectious clone of TYLCV-To (JQ013089) was constructed and agroinoculated into Nicotiana benthamiana, Nicotiana tabacum var. Xanthi, Petunia hybrida, Capsicum annuum, and Lycopersicon esculentum cv. Hausumomotaro. Agroinfection with a dimeric infectious clone of TYLCV-To induced severe leaf curling and stunting symptoms in these plants, excluding C. annuum. Tomato plants then developed typical yellow leaf curl symptoms.

• The Plant Pathology Journal
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• v.21 no.2
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• pp.167-171
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• 2005

We isolated the Cucumber green mottle mosaic virus(CGMMV) particles from watermelon leaves and designated as CGMMV-HY1 as a watermelon isolate and attempted to characterize the pathogenic isolate responsible for such an epidemic in watermelon and also to monitor dominant viral isolates in greenhouse. The watermelon plants infected with CGMMV generally showed mottle mosaic, mosaic, growth stunting, necrosis and deformed fruit. The reactions of indicator plants to CGMMV-HY1 were the local lesions on Nicotiana tabacum cv. White Burley, Nicotiana tabacum cv. Samsun, and Chenopodium amaranticola, and the mosaic symptoms only on Cucumis sativus, but the CGMMV-HY1 did not infect Nicotiana sylvesytis, Datura stramonium, Chenopodium quinoa, and Petunia hybrida. Purified virus particles were rod-shaped and about 300 nm long. The coat protein (CP) of purified CGMMV-HY1 was single band with molecular weight of about 16.5 kDa which was confirmed by western blot analysis probed with monoclonal antibody of CGMMV-HY1. The genomic and subgenomic RNAs of 6.4 kb and 0.75 kb were revealed by the electrophoresis on 1.2% formaldehydedenatured agarose gel. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequences. A 464bp of CP gene of CGMMV-HY1 was amplified by RT-PCR and cloned into PGEM-T easy vector. The nucleotide sequence of CP gene of CGMMV-HY1 shared 98%, 99%, and 100% identities with that of CGMMV strains W, KOM, and KW respectively. Based on these results, we identified CGMMV-HY1 as a CGMMV isolate of watermelon, a member of Tobamovirus.

• Journal of the Korean Institute of Landscape Architecture
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• v.15 no.2
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• pp.1-21
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• 1987

This study was conducted to find out the fundamentals for proper use of Planting system in the elementary school landscape. For this study, 70 text books were applied to quote the plants, planting situation of 15 elementary schools was surveyed, and 1,464 questionnaires were collected. The resultes and some proposals obtained are summarised as follows ; 1. Total of 177 plant species were quoted over all text books of the elementary school course. Among them, the woody species showed the highest in number with 58 and herbaceous were the next with 30, the other were vegetable of 29, food crops, weeds, industrial ornamentals crops and water plants in order. They were common species in this country and were easy to cultivate except few. 2. 210 species were planted at the 15 elementary schools in Mokpo. It was constituted of 77 of herbaceous species and 133 of woody species but localized Plants were little. The herbaceous plant frequently used were Chrysanthemum morifolium, Targests erecta, Salvia splendens, Petunia hybrida, Cosmos bipinnatus, Celosia cristata, Canna generalis, et. On the other hand in the woody species Juniperus chinensis var. kaizuka, Cerdus deodora, Camellia japonica, and in the shrub Serissa japonica, Thuja orientalis, Euonymus japonica, Buxus mrcrophylla var. koreana etc in order. Almost the same species of woody plants described above were planted in zone of I -IV. Therefore, many problems were found in aspect of aesthetics, and their function and growth. There was much differences in number of species and quantity of plants in each elementary school. However they were not associated with school size, location, and age of schools. Most of elementary schools did not have same the plants as they were represented in text brooks. Therefore, in view of educational purpose it needs to plant more species which quoted in text books.