• 한국어병학회지
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• 제5권2호
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• pp.153-158
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• 1992

남해안에 위치한 원유저장기지내의 각 정점으로 부터 원유분해세균을 분리하여 동정한 결과 Pseudomonas fluorescens, Acinetobacter baumanii, Pseudomonas maltophila, Pseudomonas aeruginosa 등으로 동정되었다. 원유를 탄소원으로 첨가한 최소배지상에서 원유분해능을 나타내었으며, 0.5%에서 6%까지의 NaCl 농도변화에 대한 원유분해능을 비교한 결과 5종의 원유분해균이 NaCl의 농도가 증가하였을 경우에는 원유분해능이 상실됨을 확인하였다. 또한 원유는 복잡한 유기화합물로 구성이 되어 있어 분리된 원유분해균이 n-decane, n-hexane, n-octane, n-dodecane 등의 간단한 탄화수소를 자화하는지를 확인하였다. 분리된 원유분해균이 plasmid를 가지는 지를 확인한 결과 Pseudomonas fluorescens 및 Pseudomonas aeruginosa 등에서 분자량이 매우 큰 plasmid가 존재함을 확인하고 ampicillin, tetracycline 및 chloramphenicol등의 항생제에 대한 내성을 조사한 결과 내성을 나타내어 원유분해에 관련된 유전자와 항생제내성을 나타내는 유전자가 plasmid상에 존재할 가능성이 높음을 알수 있다.

• Journal of Microbiology
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• 제44권3호
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• pp.354-359
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• 2006

This study examined the capacity of immobilized bacteria to degrade petroleum hydrocarbons. A mixture of hydrocarbon-degrading bacterial strains was immobilized in alginate and incubated in crude oil-contaminated artificial seawater (ASW). Analysis of hydrocarbon residues following a 30-day incubation period demonstrated that the biodegradation capacity of the microorganisms was not compromised by the immobilization. Removal of n-alkanes was similar in immobilized cells and control cells. To test reusability, the immobilized bacteria were incubated for sequential increments of 30 days. No decline in biodegradation capacity of the immobilized consortium of bacterial cells was noted over its repeated use. We conclude that immobilized hydrocarbon-degrading bacteria represent a promising application in the bioremediation of hydrocarbon-contaminated areas.

• 한국지하수토양환경학회지:지하수토양환경
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• 제18권2호
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• pp.1-9
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• 2013

The landfarming treatment for the remediation of the petroleum contaminated soil at the returned U.S. Military bases was investigated in this study. Specifically, the bioaugmentation performance using various commercially available petroleum-degrading bacteria was evaluated and the directions for enhancing the performance of the landfarming treatment were suggested. The environmental factors of the soils at the returned U.S. Military bases chosen for remediation indicate that the landfarming treatment can be used as the remediation technique; however, the addition of nitrogen or phosphorus is required. The lab-scale landfarming treatment tests using the model soil and the site soil showed that the degradation efficiency was greater with the model soil than the site soil and that the treatment performance was not affected by the number of bacteria present in the soil in the range of $10^6-10^{12}$ CFU/g. These results suggest that the successful landfarming treatment depends on the petroleum degradability of bacteria used and the environmental conditions during the treatment rather than the number of petroleum-degrading bacteria used.

• 미생물학회지
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• 제26권4호
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• pp.339-347
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• 1988

The rate of bacterial degradation of hydrocarbon was estimated for the measurment of the self-purification capacity of the aquatic ecosystem. Strain ND601P-2, selected as petroleum degrading bacteria from Nakdong River Estuary with high degradability of petroleum, transformed 42% of hexadecane to $CO_{2}$ or cell mateials under the conditions of $25^{\circ}C$, 0.03M NaCl, 167mg-$NH_{4}^+/1, 950 mg-PO_{4}^{3-}$/1, 50 mg-hexadecane/1. The mineralization rate was found to be significantly affected by the temperature and the $Q_{10}$ value was 2.2. Teh optimal salinity of the strain ND601P-2 was 2o/oo. The increased salinity caused the elevation of % respiration value and the prolonged lag phase.

• 미생물학회지
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• 제30권3호
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• pp.187-192
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• 1992

서해 경기만의 6개 정점을 대상으로 1990년 3월부터 1991년 10월까지 총 8외에 걸처 석유 분해세균의 공간적, 시간적 분포를 조사하였다. 또한, 서로 다른 배양 조건하에서의 해양 세균 군집의 석유 뷴해능도 연구하였다. 연구 기간 중, 종속 영양세균수와 석유분해세균수는 각각 7,000-108,000 CFU/ml, 0-2, 800 MPN/100 ml 로 측정되었다. 석유분해세균의 공간적 분포는 석유 탄화수소의 존제에 의하여 심하게 영향을 받은 것으로 나타났다. 실혐실내 실험에서, 석유 분해는 배양액에 yeast extract, cell free extract 의 첨가 그리고 석유뷴해세균의 접종에 의하여 증진되었다.

• 미생물학회지
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• 제30권5호
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• pp.366-370
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• 1992

1988. 7.31.~8. 10 동안 남해해역의 북위 $32^{\circ}C$30'~$34^{\circ}C$30', 동경 $123^{\circ}$30'~$128^{\circ}C$30' 내에서 정점 0101-1921로부터의 퇴적토 시료를 채취하여 총 종속영양 세균, 담수 종속영양 세규, 탄화수소 분해세균의 분포를 조사하였다. 각각의 분포는 $3{\times}10^{5}~9.2{\times}10^{6}CFU/cm^{3}$ sedment, $3{\times}10^{3}~2.1{\times}10^{6}CFU/cm^{3}$ sediment, $2{\times}10^{4}~6.2{\times}10^{6}CFU/cm^{3}$ sediment 범위로 나타났다. 총 종속영양 세균에 대한 탄화수소 분해세균의 백분율은 0.1-73.2% 로 다른 해양 퇴적토에서 보고된 값보다 높았다. 한편 상관관계 분석을 통하여 총 종속영양 세균에 대한 탄화수소 분해세균 비율과 담수 종속영양 세균 비율 사이에 r=0.60058 (n= 34) 의 상관계수를 보였고 99.9% 수준 이상에서 유의성을 나타냈다. 이와 같은 결과는 해양에 분포하는 담수 종속영양 세균이 해양으로 유입되는 담수의 영향을 받는 것과 같이 탄화수소 분해세균의 분포 또한 육지로부터 유입되는 담수의 영양을 받는 것을 시사한다. 즉 남해해역 퇴적토의 탄화수소 분해세균 분포는 강을 통하여 육지로부터 해양으로 공급되는 석유 탄화수소에 의한 영향을 받는 것으로 사료된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권5호
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• pp.35-44
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• 2014

In this study, the bio-phytoremediation and phytoremediation technologies were applied to the soils contaminated with total petroleum hydrocarbons (TPH) and heavy metals to evaluate the remediation efficacy from May 2012 to December 2013. Poplar (Populus bonatii Levl.) and Sun Hemp (Crotalaria juncea L.) were selected and planted in phytoremediation practice. These plants were also utilized in the bio-phytoremediation practice, with the addition of earthworm (Eisenia fetida) and petroleum-degrading bacteria (Pseudomonos sp. NKNU01). Furthermore, physiological characteristics, such as photosynthesis rate and maximal photochemical yield, of all testing plants were also measured in order to assess their health conditions and tolerance levels in adverse environment. After 20 months of remedial practice, the results showed that bio-phytoremediation practice had a higher rate of TPH removal efficacy at 30-60 cm depth soil than that of phytoremediation. However, inconsistent results were discovered while analyzing the soil at 100 cm depth. The study also showed that the removal efficiency of heavy metals was lower than that of TPH after remediation treatment. The results from test field tissue sample analysis revealed that more Zinc than Chromium was absorbed and accumulated by the tested plants. Plant height measurements of Poplar and Sun Hemp showed that there were insignificant differences of growth between the plants in remediation plots and those in the control plot. Physiological data of Poplar also suggested it has higher tolerance level toward the contaminated soils. These results indicated that the two testing plants were healthy and suitable for this remediation study.

• 미생물학회지
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• 제28권4호
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• pp.324-330
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• 1990

The distribution of physicochemical environmental factors and microbiological factors was studied at 6 sampling sites in Kyeongge Bay of Yellow Sea from October 1989 to October 1990. The total bacterial number, saprophytic bacterial number, petroleum-degrading bacterial number, bacterial biomass, and bacterial secondary production were measured in the range of 0.09~1.24*10$^{7}$ cells/ml, 7~60000 CFUs/ml, 0~240 cells/ml, 14.16~301 .$\mu$g-C/l, and 0.13~11.82 mg-C/m$^{3}$/hr, respectively. The turnover times of $^{3}$H-glucose and $^{3}$H-acetate were in range of 6.5~6984 and 41~24897 hours, respectively. The spatial distribution of heterotrophic bacterial communities were hightly affected by influx of organic pollutants from the coastal area and the seawater exchange with offshore.

• 한국토양환경학회지
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• 제1권2호
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• pp.35-42
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• 1996

포항근해의 정점을 대상으로 1995년 4월부터 1996년 1월까지 3개월 간격으로 종속영양세균과 원유분해세균의 분포를 조사하였다. 또한, 분리된 원유분해세균의 단일 및 혼합배양을 통하여 원유분해능도 조사하였다. 종속영양세균의 분포는 4.1 $\times$ $10^4$ - 1.2 $\times$ $10^5$ CFU/$m\ell$ 이었으며 종속영양세균에 대한 원유분해세균의 분포비는 0.05 -0.54%로서 지금까지 보필된 타지역보다 낮은 수치였다. 이 결과에서 포항근해의 원유분해세균의 분포는 육지로부터 유입되는 석유계 탄화수소에 의하여 크게 영향을 받는것으로 사료된다. 원유분해능이 우수한 26 균주를 분리.동정한 결과 Acinetobacter spp., Bacillus spp., Citrobacter spp., Micrococcus spp., Moraxella spp., Rhodococcus spp., 그리고 Berratia spp. 등의 7개 속이 나타났고, 장내세균의 출현은 해수내 오수의 유입을 반영하고 있었다. 또한 Bacillus 속 균주가 포항근해의 원유분해세균의 우점종으로 밝혀졌다. 원유분해세균을 이용한 플라스크 배양에서 원유분해는 혼합배양에 의해 증가하였다.

• 한국미생물·생명공학회지
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• 제41권1호
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• pp.79-87
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• 2013

An unstable yet efficient phenanthrene-degrading bacterium strain Ph-3 was isolated from a petroleum-contaminated site at the Mathura Oil Refinery, India. The strain was identified as Pseudomonas sp. using a polyphasic approach. An analysis of the intermediates and assays of the degradative enzymes from a crude extract of phenanthrene-grown cells showed a novel and previously unreported pattern of 1, 2-dihydroxy naphthalene and salicylic acid production. While strain Ph-3 lost its phenanthrene- degrading potential during successive transfers on a rich medium, it maintained this trait in oligotrophic soil conditions under the stress of the pollutant and degraded phenanthrene efficiently in soil microcosms. Although the maintenance and in vitro study of unstable phenotypes are difficult and such strains are often missed during isolation, purification, and screening, these bacteria constitute a substantial fraction of the microbial community at contaminated sites and play an important role in pollutant degradation during biostimulation or monitored natural attenuation.