• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6279-6284
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• 2015

Opisthorchis viverrini (OV)-induced cholangiocarcinoma (CCA) is an important cancer in the Great Mekong region, particularly in Thailand. Limitations of treatment options and the lack of an effective diagnostic tool for early detection of CCA are major concerns for the control of this type of cancer. The aim of the study was to investigate anti-CCA activity of the ethanolic extract of Atractylodes lancea (Thunb.) DC., and the applicability of positron emission tomography-computed tomography (PET-CT) as a tool for detection and monitoring the progression of CCA in Opisthorchis viverrini (OV)/dimethylnitrosamine (DMN)-induced CCA hamsters. Male Syrian hamsters were used for toxicity tests and anti-CCA activity evaluation. Development of CCA was induced by initial feeding of 50 metacercariae of OV, followed by drinking water containing 12.5 ppm of DMN in hamsters. The ethanolic extract of A. lancea (Thunb.) DC. was administered orally for 30 days. PET-CT was performed every 4 weeks after initiation of CCA using 18F-fluorodeoxyglucose ($^{18}F-FDG$). Results from the present study suggest that the ethanolic extract of A. lancea (Thunb.) DC. rhizome exhibited promising anti-CCA activity and safety profile in the OV/DMN-induced hamster model. To successfully apply PET-CT as a tool for early detection of tumor development and progression, modification of radiolabeling approach is required to improve its specificity for CCA cells.

• 한국컴퓨터정보학회논문지
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• 제27권2호
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• pp.25-32
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• 2022

본 논문에서는 활동 인식장치를 이용한 딥러닝 기반의 반려동물 모니터링 시스템을 제안한다.이 시스템은 반려동물의 활동 인식장치와 반려인의 스마트 기기, 서버로 구성된다. 아두이노 기반 활동 인식 장치로부터 가속도와 자이로 데이터를 수집하고, 이로부터 반려동물의 걸음 수를 연산하였다. 수집된 데이터는 전처리 과정을 거쳐 CNN과 LSTM을 하이브리드한 딥러닝 모델을 통해 5가지 형태(앉기, 서기, 눕기, 걷기, 뛰기)로 활동을 인식함으로써 활동량을 측정한다. 마지막으로, 반려인의 스마트 기기에 일일 및 주간 브리핑 차트 등 활동 변화에 대한 모니터링을 제공한다. 성능 평가 결과, 반려동물의 구체화된 활동 인식 및 활동량 측정이 가능함을 확인하였다. 향후 데이터 축적을 통해 반려동물의 이상행동 탐지 및 헬스 케어 서비스의 확장을 기대할 수 있다.

• 대한의용생체공학회:의공학회지
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• 제32권1호
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• pp.74-78
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• 2011

Although recent studies have shown the usibility of [F-18]FDG small animal Positron Emission Tommography (PET) as a functional neuroimaging technique in behavioural small animal study, researches showing the detection power of functional changes in the brain are still limited. Thus, in the study, we performed [F-18]FDG small animal PET neuroimaging in the well-established fear behavioural experiment. Twelve rats were exposed on cat for 30 minutes after the [F-18]FDG injection. As a result, the brain activity in bilateral amygdala areas significantly increased in the fear condition. In addition, the fear condition evoked the functional activities of hypothalamus, which seemed to be related to the response to stress. These clear localization of fear related brain regions may reflect that a functional neuroimaging technique using [F-18]FDG small animal PET has functional detectibility enough to be applied in small animal behavioral research.

• 대한핵의학회지
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• 제31권1호
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• pp.83-89
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• 1997

CT에서도 악성 여부가 불명확한 단일 폐결절의 감별진단에 있어 PET이 어떠한 도움을 줄 수 있는지 알아보고자 이러한 환자 30명을 대상으로 FDG-PET을 시행하여 각 환자에서 구한 pSUV, aSUV, TAC 양상, 50/10비의 4가지 매개변수를 조직검사결과와 비교하여 다음과 같은 성적을 얻었다. 1) 4가지 매개변수의 악성판정 기준치로 $pSUV{\geq}3.5$, aSUV>2.5, TAC=상향, 50/10비${\geq}1.45$를 적용하였을 때 악성판정에 있어 각각의 예민도는 86.7%, 66.6%, 86.7%, 73.3%였으며, 특이도는 86.7%, 86.7%, 46.7%, 86.7%였다. 2) 4가지 매개변수중 구하기 용이한 점과 정확도를 감안할 때 임상적으로 pSUV가 가장 유용하였다. 3) pSUV를 사용하였을 경우 CT에서 불확실한 단일 폐결절의 87%에서 정확한 진단이 가능하였다. 4) 기관지 폐포암을 제외할 경우 예민도가 100%였다. 이상의 결과에서 FDG-PET에서 구한 pSUV는 여러 가지 매개변수중 가장 용이하게 구할 수 있으며 비 침습적으로 단일 폐결절의 악성여부를 가릴 수 있는 유용한 방법이었다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 춘계학술대회
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• pp.141-143
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• 2021

The Activity patterns of animal species are difficult to access and the behavior of freely moving individuals can not be assessed by direct observation. As it has become large challenge to understand the activity pattern of animals such as dogs, and cats etc. One approach for monitoring these behaviors is the continuous collection of data by human observers. Therefore, in this study we assess the activity patterns of dog using the wearable sensors data such as accelerometer and gyroscope. A wearable, sensor -based system is suitable for such ends, and it will be able to monitor the dogs in real-time. The basic purpose of this study was to develop a system that can detect the activities based on the accelerometer and gyroscope signals. Therefore, we purpose a method which is based on the data collected from 10 dogs, including different nine breeds of different sizes and ages, and both genders. We applied six different state-of-the-art classifiers such as Random forests (RF), Support vector machine (SVM), Gradient boosting machine (GBM), XGBoost, k-nearest neighbors (KNN), and Decision tree classifier, respectively. The Random Forest showed a good classification result. We achieved an accuracy 86.73% while the detecting the activity.

• Korean Journal of Radiology
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• 제23권9호
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• pp.911-920
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• 2022

Objective: ⁶⁸Ga-NGUL is a novel prostate-specific membrane antigen (PSMA)-targeting tracer based on Glu-Urea-Lys derivatives conjugated to a 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA) chelator via a thiourea-type short linker. This phase I clinical trial of ⁶⁸Ga-NGUL was conducted to evaluate the safety and radiation dosimetry of ⁶⁸Ga-NGUL in healthy volunteers and the lesion detection rate of ⁶⁸Ga-NGUL in patients with prostate cancer. Materials and Methods: We designed a prospective, open-label, single-arm clinical trial with two cohorts comprising six healthy adult men and six patients with metastatic prostate cancer. Safety and blood test-based toxicities were monitored throughout the study. PET/CT scans were acquired at multiple time points after administering ⁶⁸Ga-NGUL (2 MBq/kg; 96-165 MBq). In healthy adults, absorbed organ doses and effective doses were calculated using the OLINDA/EXM software. In patients with prostate cancer, the rates of detecting suspicious lesions by ⁶⁸Ga-NGUL PET/CT and conventional imaging (CT and bone scintigraphy) during the screening period, within one month after recruitment, were compared. Results: All 12 participants (six healthy adults aged 31-32 years and six prostate cancer patients aged 57-81 years) completed the clinical trial. No drug-related adverse events were observed. In the healthy adult group, ⁶⁸Ga-NGUL was rapidly distributed, with the highest uptake in the kidneys. The median effective dose coefficient was calculated as 0.025 mSv/MBq, and cumulative activity in the bladder had the highest contribution. In patients with metastatic prostate cancer, 229 suspicious lesions were detected using either ⁶⁸Ga-NGUL PET/CT or conventional imaging. Among them, ⁶⁸Ga-NGUL PET/CT detected 199 (86.9%) lesions and CT or bone scintigraphy detected 114 (49.8%) lesions. Conclusion: ⁶⁸Ga-NGUL can be safely applied clinically and has shown a higher detection rate for the localization of metastatic lesions in prostate cancer than conventional imaging. Therefore, ⁶⁸Ga-NGUL is a valuable option for prostate cancer imaging.

• BMB Reports
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• 제55권6호
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• pp.267-274
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• 2022

Molecular imaging is used to improve the disease diagnosis, prognosis, monitoring of treatment in living subjects. Numerous molecular targets have been developed for various cellular and molecular processes in genetic, metabolic, proteomic, and cellular biologic level. Molecular imaging modalities such as Optical Imaging, Magnetic Resonance Imaging (MRI), Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), and Computed Tomography (CT) can be used to visualize anatomic, genetic, biochemical, and physiologic changes in vivo. For in vivo cell imaging, certain cells such as cancer cells, immune cells, stem cells could be labeled by direct and indirect labeling methods to monitor cell migration, cell activity, and cell effects in cell-based therapy. In case of cancer, it could be used to investigate biological processes such as cancer metastasis and to analyze the drug treatment process. In addition, transplanted stem cells and immune cells in cell-based therapy could be visualized and tracked to confirm the fate, activity, and function of cells. In conventional molecular imaging, cells can be monitored in vivo in bulk non-invasively with optical imaging, MRI, PET, and SPECT imaging. However, single cell imaging in vivo has been a great challenge due to an extremely high sensitive detection of single cell. Recently, there has been great attention for in vivo single cell imaging due to the development of single cell study. In vivo single imaging could analyze the survival or death, movement direction, and characteristics of a single cell in live subjects. In this article, we reviewed basic principle of in vivo molecular imaging and introduced recent studies for in vivo single cell imaging based on the concept of in vivo molecular imaging.

• 대한핵의학회지
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• 제33권1호
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• pp.1-10
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• 1999

Cancer cells are known to show increased rates of glycolysis metabolism. Based on this, PET studies using F-18-fluorodeoxyglucose have been used for the detection of primary and metastatic tumors. To account for this increased glucose uptake, a variety of mechanisms has been proposed. Glucose influx across the cell membrane is mediated by a family of structurally related proteins known as glucose transporters (Gluts). Among 6 isoforms of Gluts, Glut-1 and/or Glut-3 have been reported to show increased expression in various tumors. Increased level of Glut mRNA transcription is supposed to be the basic mechanism of Glut overexpression at the protein level. Some oncogens such as src or ras intensely stimulate Glut-1 by means of increased Glut-1 mRNA levels. Hexokinase activity is another important factor in glucose uptake in cancer cells. Especially hexokinase type II is considered to be involved in glycolysis of cancer cells. Much of the hexokinase of tumor cells is bound to outer membrane of mitochondria by the porin, a hexokinase receptor. Through this interaction, hexokinase may gain preferred access to ATP synthesized via oxidative phosphorylation in the inner mitochondria compartment. Other biologic factors such as tumor blood flow, blood volume, hypoxia, and infiltrating cells in tumor tissue are involved. Relative hypoxia may activate the anaerobic glycotytic pathway. Surrounding macrophages and newly formed granulation tissue in tumor showed greater glucose uptake than did viable cancer cells. To expand the application of FDG PET in oncology, it is important for nuclear medicine physicians to understand the related mechanisms of glucose uptake in cancer tissue.

• 한국콘텐츠학회논문지
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• 제8권12호
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• pp.236-245
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• 2008

현대 의학은 과학과 더불어 놀라운 만큼 발전을 거듭하고 있기에 각종 암 환자의 조기 발견으로 인하여 인간의 생명 연장에 큰 공헌을 하고 있다. 조기 발견의 일환으로 시행되는 핵의학 검사 중에서 Bone scan을 통한 암 환자의 전이 여부와 전이되는 시기, 정도를 연구함으로서 암 발견의 초기와 말기의 차이뿐만 아니라 그 시기를 추정한 데이터로 적극적 치료를 수행할 수 있기를 기대하고자 한다. 더불어 암 판정을 받은 환자는 그 시기를 더 지연시킬 수 있는 필요적 방어를 수행할 수 있도록 최대한 지도가 가능하리라 판단이 된다. 암 판정의 초기에는 부단한 노력과 투자로 검사와 치료를 병행하다가 일정시간이 경과하고 난 후에는 다소 소홀해지는 경우가 빈번하게 나타남을 알 수 있었으며, 그로 인해 암세포의 전이가 활발하게 진행되어짐을 확정할 수 있었다. 이는 치료 목적을 가지고 대하는 각 의료기관 및 의료기관 종사자에게 있어 간과 할 수 있는 부분이 아니므로 보다 적극적 대처를 요하는 사안이다.