• 대한수의학회지
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• 제39권2호
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• pp.247-256
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• 1999

A disease of young Holstein calves characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis with bacterial overgrowth, periodontitis, delayed wound healing, persistent neutrophilia and death at an early age had been originally described in 1983 and again in 1987. Most of these calves had stunted growth and a persistent, progressive neutrophilia (often exceeding 100,000/ml). By investigation of pedigrees, all of the affected calves have now been traced to a common sire and confirmed by polymerase chain reaction (PCR) diagnostic DNA testing to be homozygous carriers of a defective allele for bovine CD18. Neutrophils from these calves have several functional deficits and, most importantly, fail to adhere in a ${\beta}_2$-integrin dependent manner. The ${\beta}_2$-integrins represent a family of glycoproteins which participate in various leukocyte adhesion reactions during host defense. The presence or absence of ${\beta}_2$-integrin molecules can be demonstrated on the surface of neutrophils, monocytes and lymphocytes from normal or affected calves using specific monoclonal antibodies and flow cytometry, or by colloidal gold immunolabeling and scanning electron microscopy in backscatter mode. Deficiency of the ${\beta}_2$-integrins on all leukocyte types in Holstein calves is analogous to leukocyte adhesion deficiency (LAD) seen in humans. Neutrophils in bovine (BLAD) and human LAD patients are unable to adhere to the endothelial lining of the cardiovascular system thus interrupting egression of neutrophils into infected tissues. Other leukocytes, while still deficient in expression of the ${\beta}_2$-integrins, are still able to efficiently egress from the blood stream due to interactions of other adhesion molecules that are not as highly expressed on neutrophils. Both BLAD cattle and LAD children (who do not receive bone marrow transplants) often die at an early age as a result of the failure of neutrophils to extravasate into infected tissues. In 1991, Shuster, et $al^{27}$, identified two point mutations within the alleles encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency. One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in an extracellular region of this adhesion glycoprotein that is highly conserved (> 95% identity) between humans, cattle and mice. The other mutation is silent. Numerous calves with clinical symptoms of leukocyte adhesion deficiency have since been tested and all have been found homozygous for the D128G allele. In addition, calves homozygous far the D128G allele have been identified during widespread DNA testing in the United States. All cattle with the mutant allele are related to one bull, who through artificial insemination (A.I.), sired many calves in the 1950's and 1960's. The carrier frequency of the D128G CD18 allele among U.S. Holstein cattle had reached approximately 15% among active A.I. bulls and 8% among cows. By 1993, the organization of the dairy industry and the diagnostic test developed to genotype cattle, enabled virtually complete eradication of bovine leukocyte adhesion deficiency among current and future A.I. bulls.

• 한국콘텐츠학회논문지
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• 제21권10호
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• pp.59-66
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• 2021

본 논문에서는 통합보안관제에 인공지능 기술을 적용하고, 기존 보안관제와 인공지능 보안관제의 대응절차를 일원화한, 개선된 통합보안관제 절차를 새롭게 제안하였다. 현재의 사이버보안관제는 사람의 능력 수준에 의존도가 매우 높다. 그래서 사람에 의해 여러 이기종 장비에서 발생하는 다양한 로그를 분석하고, 급증하는 보안이벤트를 모두 분석·처리한다는 것은 사실상 무리가 있다. 그리고 문자열과 패턴 일치로 탐지하는 시그니처 기반의 보안장비는 APT(Advanced Persistent Threat)와 같은 고도화·지능화된 사이버공격을 정확히 탐지하기에 기능상 부족한 면이 있다. 이러한 문제들을 해결하기 위한 방안으로 인공지능 지도·비지도학습 기술을 사이버공격 탐지 및 분석에 적용하고, 이를 통해 수 없이 많이 발생하는 로그와 이벤트의 분석을 자동화하여, 고도화된 사이버공격의 지속적인 발생을 예측·차단할 수 있도록 하여 전반적인 측면에서 대응수준을 높였다. 그리고 보안관제에 인공지능 기술을 적용한 후 AI와 SIEM의 중복 탐지 등의 문제점을 일원화 된 침해대응 프로세스(절차)로 통합·해결함으로써 개선된 통합보안관제 서비스 모델을 새롭게 제안하였다.

• 한국구조물진단유지관리공학회 논문집
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• 제22권4호
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• pp.1-9
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• 2018

일반적인 플랜트 산업에서의 파이프라인은 지지형식으로만 구조설계를 하고 있으며 시공방법이 용접에 의한 단순접합방법을 선호하고 있다. 그러나 플랜트 산업 특성상 용접 불꽃에 의한 화재발생시 매우 위험함으로 통상적인 고장력 볼트 접합을 단순화하여 내진성능이 확보되는 파이프랙 접합방식을 개선하는 연구이다. 연구결과 H-Beam 대 H-Beam의 접합기술의 향상과, 내진성능이 향상된 파이프랙 구조기술 개발, 파이프랙 제작 및 설치 시간 단축 기술 개발, 다양한 방법의 철 구조물 설치 기술 개발, 적용분야 확대에 따른 기술의 접근성 향상이라는 효과를 얻을 수 있다. 경제적 측면에서는 기존 파이프랙 공법에 비해 현장설치 공정 축소로 인건비 및 장비비 절감 효과를 기대할 수 있으며, 자재비의 경우 H-Beam 연결을 위한 고장력 볼트의 수량 감소효과 대비 대량생산을 위한 금형비용 발생으로 원가가 절감될 수 있다. 안정성 측면에는 기존의 현장설치에서 나타났던 다소 큰 위험성 요소를 최소화시킬 수 있는 공장제작 공정을 통해 접합 작업자의 안정성을 증대시킬 수 있을 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.425-432
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• 2016

In addition to classical synaptic transmission, information is transmitted between cells via the activation of extrasynaptic receptors that generate persistent tonic current in the brain. While growing evidence supports the presence of tonic NMDA current ($I_{NMDA}$) generated by extrasynaptic NMDA receptors (eNMDARs), the functional significance of tonic $I_{NMDA}$ in various brain regions remains poorly understood. Here, we demonstrate that activation of eNMDARs that generate INMDA facilitates the ${\alpha}$-amino-3-hydroxy-5-methylisoxazole-4-proprionate receptor (AMPAR)-mediated steady-state current in supraoptic nucleus (SON) magnocellular neurosecretory cells (MNCs). In $low-Mg^{2+}$ artificial cerebrospinal fluid (aCSF), glutamate induced an inward shift in $I_{holding}$ ($I_{GLU}$) at a holding potential ($V_{holding}$) of -70 mV which was partly blocked by an AMPAR antagonist, NBQX. NBQX-sensitive $I_{GLU}$ was observed even in normal aCSF at $V_{holding}$ of -40 mV or -20 mV. $I_{GLU}$ was completely abolished by pretreatment with an NMDAR blocker, AP5, under all tested conditions. AMPA induced a reproducible inward shift in $I_{holding}$ ($I_{AMPA}$) in SON MNCs. Pretreatment with AP5 attenuated $I_{AMPA}$ amplitudes to ~60% of the control levels in $low-Mg^{2+}$ aCSF, but not in normal aCSF at $V_{holding}$ of -70 mV. $I_{AMPA}$ attenuation by AP5 was also prominent in normal aCSF at depolarized holding potentials. Memantine, an eNMDAR blocker, mimicked the AP5-induced $I_{AMPA}$ attenuation in SON MNCs. Finally, chronic dehydration did not affect $I_{AMPA}$ attenuation by AP5 in the neurons. These results suggest that tonic $I_{NMDA}$, mediated by eNMDAR, facilitates AMPAR function, changing the postsynaptic response to its agonists in normal and osmotically challenged SON MNCs.

• 한국해양학회지:바다
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• 제26권2호
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• pp.63-81
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• 2021

이 연구에서는 2018년 늦은 봄철을 대상으로, 제주도내 위치한 제주와 보목 하수처리장의 해양방류구 주변 해역의 하수처리 방류수의 이동 특성을 파악하기 위하여 다층 유향·유속계 (Acoustic Doppler Current Meter; ADCP)와 지역규모 해양 수치모델의 가상 추적자 실험을 활용한 분석을 수행하였다. ADCP관측 결과에 의하면 두 하수처리장 모두 방류구 인근 해역의 해수 유동은 비조석 성분 보다 조석 성분이 컸고, 조류는 등수심선과 평행한 방향의 왕복성 운동이 지배적이었다. 조화 분석결과는 제주와 보목 하수처리장 해역 모두 반일주기 유속 성분이 우세한 혼합형 조석특성이 지배적이지만, 보목 하수처리장 주변의 유속 세기가 제주 하수처리장 유속의 50% 정도로 느리고, 조류의 회전성이 제주는 시계 방향, 보목의 경우 반시계 방향으로 지역적으로 차이를 보인다. 특히, 보목 하수처리장 방류구 주변해역은 상대적으로 느린 유속과 더불어 잔차류가 등수심선을 가로질러 연안으로 향하기 때문에 해안을 따라 오염물질이 집적되는데 유리한 환경이다. 고해상도 수치실험은 유속·유향 현장 관측 결과와 유사한 해수 유동 특성을 잘 모의하였으며, 오염물질의 이동확산을 파악하기 위한 추적자 실험을 통하여 잔차성분이 연안을 향하는 보목 하수처리장 연안에서 오염물질 잔류량이 상대적으로 높아질 수 있음을 제시하였다. 이러한 결과는 늦은 봄철에 보목 하수처리장 인근 해역이 상대적으로 약한 조류와 연안을 향하는 잔차성분의 영향으로 하수처리 방류수에 의한 연안 오염 가능성이 높은 환경임을 제시하는 결과이다. 퇴적유기물 조사에서도 보목하수처리장 인근 연안은 중간오염 단계를 보임으로써 조석 잔자류에 의한 연안 오염물질 축적과 그에 따른 연안환경오염의 가능성을 뒷받침하고 있다.

• 한국심리학회지 : 문화 및 사회문제
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• 제24권1호
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• pp.1-27
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• 2018

최근 학교폭력의 지속성과 가해와 피해의 악순환이 문제로 떠오르고 있어 장기적이고 다각적인 접근이 시행되어야 할 필요성이 제기되고 있다. 따라서 본 연구는 지속적 가해나 피해를 이끄는 요인으로 이전 시점의 가해와 피해경험에 주목하였으며, 가해피해자 집단에게서 살펴볼 수 있는 역동성은 상호적 인과관계를 통해 살펴보고자 하였다. 또한 이러한 관계에서 성별의 차이가 나타나는 지를 함께 검증하였다. 본 연구는 한국아동·청소년패널조사(KCYPS)의 초등학교 4학년 패널 3차년도부터 6차년도 자료를 활용하였다(N = 1,881). 가해경험과 피해경험 간 관계를 알아보기 위해 자기회귀교차지연 모형을 활용하였고 성별의 차이를 검증하기 위해 다집단 분석을 함께 실시하였다. 본 연구의 결과를 요약하면 다음과 같다. 첫째, 학교폭력 가해경험과 피해경험이 시간에 따라 안정적으로 지속되는 것을 확인하여 이전 시점의 가해경험과 피해경험이 다음 시점의 가해경험과 피해경험을 각각 예측하는 것으로 나타났다. 둘째, 학교폭력 가해경험과 피해경험의 역동성은 유의하지 않게 나타나 이전 시점의 가해경험과 피해경험이 각각 이후 시점의 피해경험과 가해경험을 예측하지 않는 것으로 나타났다. 이에 본 연구에서는 학교폭력 예방과 개입 방안이 단기간에 효과를 내는 일시적 프로그램으로 제공되기 보다는 청소년의 발달적 특성과 학교폭력의 양상을 고려하는 하나의 과정으로써 만들어져야 한다는 것을 제안하고 있다.

• International Journal of Oral Biology
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• 제31권4호
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• pp.113-118
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• 2006

In the primary sensory neuron of the mesencephalic trigeminal nucleus (MTN), the peripheral axon supplies a large number of annulospiral endings surrounding intrafusal fibers encapsulated in single muscle spindles while the central axon sends only a few number of synapses onto single ${\alpha}-motoneurons({\alpha}-MNs)$. Therefore, the ${\alpha}-{\gamma}$ linkage is thought to be very crucial in the jaw-closing movement. Spike activity in a ${\gamma}-motoneuron\;({\gamma}-MN)$ would induce a large number of impulses in single peripheral axons by activating many intrafusal fibers simultaneously, subsequently causing an activation of ${\alpha}-MNs$ in spite of the small number of synapses. Thus, the activity of ${\gamma}-MNs$ may be vital for modulation of jaw-closing movements. Independently of such a spindle activity modulated by ${\gamma}-MNs$, somatic depolarization in MTN neurons is known to trigger the oscillatory spike activity. Nevertheless, the trafficking of these spikes arising from the two distinct sources of MTN neurons is not well understood. In this short review, switching among multiple functional modes of MTN neurons is discussed. Subsequently, it will be discussed which mode can support the ${\alpha}-{\gamma}$ linkage. In our most recent study, simultaneous patch-clamp recordings from the soma and axon hillock revealed a spike-back-propagation from the spike-initiation site in the stem axon to the soma in response to a somatic current pulse. The persistent $Na^+$ current was found to be responsible for the spike-initiation in the stem axon, the activation threshold of which was lower than those of soma spikes. Somatic inputs or impulses arising from the sensory ending, whichever trigger spikes in the stem axon first, would be forwarded through the central axon to the target synapse. We also demonstrated that at hyperpolarized membrane potentials, 4-AP-sensitive $K^+$ current ($IK_{4-AP}$) exerts two opposing effects on spikes depending on their origins; the suppression of spike initiation by increasing the apparent electrotonic distance between the soma and the spike-initiation site, and the facilitation of axonal spike invasion at higher frequencies by decreasing the spike duration and the refractory period. Through this mechanism, the spindle activity caused by ${\gamma}-MNs$ would be safely forwarded to ${\alpha}-MNs$. Thus, soma spikes shaped differentially by this $IK_{4-AP}$ depending on their origins would reflect which one of the two inputs was forwarded to the target synapses.

• 대한족부족관절학회지
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• 제25권4호
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• pp.149-156
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• 2021

Purpose: Given the lack of definite evidence-based guidelines in clinical practice, there may be a wide variation in treatment protocols for osteochondral lesions of the talus (OLT). Based on the Korean Foot and Ankle Society (KFAS) member survey, this study aimed to report the current trends in the management of OLT. Materials and Methods: A web-based questionnaire containing 30 questions was sent to all KFAS members in September 2021. The questions were mainly related to clinical experience and preferences in diagnosis, conservative, and surgical treatments for patients with OLT. Answers with a prevalence of ≥50% of respondents were considered a tendency. Results: Sixty-two (11.3%) of the 550 surgeons queried responded to the survey. The responses to 9 (30.0%) of the total of 30 questions established a tendency. Answers exhibiting a tendency were as follows; additional diagnostic tools except for plain radiograph (magnetic resonance imaging), most common conservative treatment method (oral medication, rest), most important radiological factor in decision making for surgical treatment and method (size of the lesion, ankle instability, loose bodies), most important patient factors in decision making for surgical treatment and method (age, activity or occupation), infrequently requiring posterior arthroscopy (less than 3%), most common revision surgery for failed bone marrow stimulation procedure (osteochondral autograft transplantation [OAT]), not requiring additional procedure for donor site in OAT, the main reason for unsatisfactory result after OAT (persistent pain without radiological abnormality), no generalization of autologous chondrocyte implantation or chondrogenesis using stem cells. Conclusion: This study presents updated information on current trends in the management of OLT in Korea. Both consensus and variations in the approach to patients with OLT were revealed through this survey. Since recent biologic efforts to regenerate cartilage have been unsuccessful, further studies to identify clinical evidence would be needed.

• Asian Pacific Journal of Cancer Prevention
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• 제15권9호
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• pp.3851-3864
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• 2014

Cytokine research is currently at the forefront in cancer research. Deciphering the functions of these multiple small molecules, discovered within the cell and in intercellular spaces, with their abundance and pleotrophism, was initially a great challenge. Advances in analytical chemistry and molecular biology have made it possible to unravel the pathophysiological functions of these polypeptides/proteins which are called interleukins, chemokines, monokines, lymphokines and growth factors. With more than 5 million women contracting cervical cancer every year this cancer is a major cause of mortality and morbidity the world over, particularly in the developing countries. In more than 95% of cases it is associated with human papilloma virus (HPV) infection which is persistent, particularly in those with a defective immune system. Although preventable, the mere magnitude of prevalence of HPV in the world population makes it a dominating current health hazard. The discovery of cytokine dysregulation in cervical cancer has spurted investigation into the possibility of using them as biomarkers in the early diagnosis of cases at high risk of developing cancer. Their critical role in carcinogenesis and progression of cervical cancer is now being revealed to a great extent. From diagnostics to prognosis, and now with a possible role in therapeutics and prevention of cervical cancer, the cytokines are being evaluated in all anticancer approaches. This review endeavours to capture the essence of the astonishing journey of cytokine research in cervical neoplasia.

• 한국대기환경학회지
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• 제29권5호
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• pp.513-527
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• 2013

Recently, hazardous air pollutants (HAPs) have been of great concern in Korea, largely due to public awareness on the importance of their impacts on environmental health. A group of HAPs includes a large number of various chemicals such as VOCs, PAHs, PCBs, dioxines, furans, organochlorinated pesticides, and some heavy metals. These groups of pollutants are generally known to have genetic toxicity and to be persistent in the environment. In addition, most of chemicals belong to the category of HAPs are widely distributed not only in air, but also in water and soil environments, and has therefore undergone considerable scrutiny for the last three decades. In this article, environmental implications and management of HAPs in Korea will be reviewed, with a particular emphasis on the monitoring of HAPs in the ambient air. A number of field studies will be introduced, which were recently conducted in large urban and industrial areas. Based on the filed studies, eight pollutants have been suggested to be intensively managed as higher priority pollutants, which are benzene, 1,3-butadiene, formaldehyde, acrolein, trichloroethylene, benzo(a)pyrene, hexa-valent Cr, and $PM_{2.5}$. Finally, future directions for a mega scale project for comprehensive monitoring of ambient atmosphere in large urban areas will be suggested. Such an innovative project is believed to provide more realistic information on the nature of the population exposure, which can not be simply identified by emission inventories or source investigations. Therefore, any strategy for the management of HAPs should be developed by not only emission-based approaches, but also observation-based approaches.