• 대한본초학회지
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• 제30권6호
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• pp.17-24
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• 2015

Objectives : Persicaria tinctoria belongs to the Polygonaceae family and it has been used as the natural dye traditionally. Also, it is well known that the Persicaria tinctoria is used for treating the following symptoms such as fever, inflammation and edema. The purpose of this study is to investigate the effective source of antioxidants and anti-inflammatory agent from various parts of Persicaria tinctoria.Methods : We investigated the antioxidative and anti-inflammatory properties of the Persicaria tinctoria extracts. Antioxidant activities were measured by 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, Fe²⁺ chelating activity and Reducing power of Persicaria tinctoria extracts. And its inhibitory effect against oxidative DNA damage was evaluated in non-cellular system using φX-174 RF I plasmin DNA. The anti-inflammatory effect of Persicaria tinctoria was measured by using the inhibitory efficacy for the amount of nitric-oxide (NO) produced in LPS induced RAW264.7 cells.Results : The extracts from stem part showed better DPPH scavenging activity compared to those of the leaf and root extracts. Their IC₅₀s were measured as 7.17, 144.40 and 165.07 ug/ml, respectively. These results were similar to that of ABTS radical scavenging assay and reducing power. Also, Persicaria tinctoria showed the protective effects of DNA damage against oxidative stress and anti-inflammatory effect by suppression of NO production in LPS induced RAW264.7 cells.Conclusions : These results showed that various parts of Persicaria tinctoria can be used as an effective source of antioxidants and anti-inflammatory agents via antioxidative activities and anti-inflammatory effect.

• 한국자원식물학회지
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• 제31권5호
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• pp.433-452
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• 2018

본 연구는 천연식물자원을 대상으로 치매의 일종인 알츠하이머병을 개선 및 치료할 수 있는 소재를 발굴하기 위해 식물추출물에 대해 콜린성 에스테라제(AChE) 저해활성을 탐색하였다. 184개의 식물추출물을 $100{\mu}g/ml$의 단일농도에서 아세틸콜린에스테라제(AChE)을 실험한 결과, 10% 이상의 AChE 저해활성을 보였던 추출물은 황벽나무(Phellaodendron amurense Rupr., 수피, 메탄올 추출물), 황벽나무(Phellaodendron amurense Rupr., 수피, 물 추출물), 연꽃(Nelumbo nucifera Gaertn., 수술/자방, 메탄올 추출물), 쪽(Persicaria tinctoria H. GROSS, 꽃, 메탄올 추출물), 중국황련(Coptis chinensis, 뿌리줄기, 메탄올 추출물), 육계(Cinnamomum cassia Blume, 수피, 에탄올 추출물) 및 잇꽃(Carthamus tinctorius L., 열매, 에탄올 추출물) 등 7개가 확인되었다. 선발된 7개의 추출물에 대해서는 25, 20, 100 및 $200{\mu}g/ml$의 최종농도에서 AChE 및 BuChE에 대한 저해활성을 검정하였다. 그 결과, AChE 저해활성에서는 연꽃(Nelumbo nucifera Gaertn.) 수술/자방의 메탄올 추출물이 $15.0{\pm}3.4%{\sim}73.5{\pm}3.6%$, 쪽(Persicaria tinctoria H. GROSS) 꽃의 메탄올 추출물이 $19.5{\pm}9.1%{\sim}63.5{\pm}5.4%$, 중국황련(Coptis chinensis) 뿌리줄기의 메탄올 추출물이 $81.6{\pm}0.4%{\sim}58.5{\pm}2.4%$, 황벽나무(Phellaodendron amurense Rupr.) 수피의 메탄올 추출물이 $69.9{\pm}1.8%{\sim}80.5{\pm}0.9%$, 그리고 황벽나무 (Phellaodendron amurense Rupr.) 수피의 물 추출물이 $54.8{\pm}0.6%{\sim}78.3{\pm}2.6%$의 값을 보였다. BuChE 저해활성에서는 연꽃(Nelumbo nucifera Gaertn.) 수술/자방의 메탄올추출물, 쪽(Persicaria tinctoria H.GROSS) 꽃의 메탄올추출물 및 중국황련(Coptis chinensis) 뿌리줄기의 메탄올추출물이 각각 $58.9{\pm}7.8{\sim}81.6{\pm}1.9%$ 및 $45.8{\pm}9.8%{\sim}72.4{\pm}2.5%$ 및 $33.1{\pm}9.9%{\sim}55.4{\pm}5.4%$의 순으로 높은 BuChE 저해활성을 나타내었다. 이러한 실험결과를 종합하여 볼때, 연꽃(Nelumbo nucifera Gaertn.)의 수술/자방, 쪽(Persicaria tinctoria H. GROSS)의 꽃, 중국황련(Coptis chinensis)의 뿌리줄기, 황벽나무(Phellaodendron amurense Rupr.)의 수피는 알츠하이머병(AD)의 개선 효과를 나타낼 수 있는 후보 소재로 사료되었다.

• 한국자원식물학회지
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• 제29권1호
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• pp.32-38
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• 2016

쪽 종자의 이용성 향상을 위한 기초 자료 확보 측면에서 쪽 종자의 일반성분, 지방산, 무기물, 아미노산 함량 분석과 쪽 종자의 메탄올 추출물을 이용하여 생리활성 효과를 조사하였다. 쪽 종자의 총 열량은 348.00 kcal/100 g이었으며, 일반성분은 수분 7.85%, 탄수화물 67.90%, 조단백 10.10%, 조지방 4.00%, 조회분 10.15%로 구성되어 있었다. 포화지방산의 총량은 0.905 g/100 g이었고, 불포화지방산의 총량은 2.717 g/100 g이었다. 쪽 종자 100 g에 함유된 무기질은 K (549.5 ㎎), Mg (264.4 ㎎), Ca(216.2 ㎎), Fe (12.1 ㎎), Zn (3.0 ㎎) 순으로 많았다. 구성아미노산은 총 15종이 검출되었으며, 이 중 alanine (1,432.6 ㎎/100 g)과 glutamic acid (1,088.8 ㎎/100 g)의 함량이 많았다. 총 폴리페놀 함량은 11.08 ㎎/L였고, 총 플라보노이드 함량은 3.56 ㎎/L였다. 쪽 종자 메탄올 추출물의 생리활성 중 radical scavenging activity는 1,000 ㎎/L일 때 DPPH radical 소거능 86.74%, ABTS radical 소거능 61.74%로 나타났다.

• 한국약용작물학회지
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• 제16권3호
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• pp.195-198
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• 2008

Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

• 한국약용작물학회지
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• 제21권3호
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• pp.213-219
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• 2013

This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was $0.210{\pm}0.005$, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.

• Journal of Applied Biological Chemistry
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• 제54권1호
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• pp.47-50
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• 2011

본 연구에서는 천연 미백소재 탐색을 위하여 쪽(P. tinctoria) 꽃 추출물의 tyrosinase 저해활성을 검증하였으며, 쪽 꽃 추출물은 $70.8{\pm}2.2{\mu}g/mL$의 $IC_{50}$ 값을 나타내었다. LH-20 chromatography와 HPLC분석을 통해 쪽으로부터 두 개의 활성물질을 정제하였고, 이 물질들의 구조 분석은 LC-MS와 NMR 데이터 해석을 바탕으로 진행하여 두 가지 화합물은 각각 quercetin-3-Orhamnoside (Q3R), myricetin-3-O-rhamnoside (M3R)로 밝혀졌다. Q3R과 M3R은 순서대로 $47.0{\pm}0.1$, $150.5{\pm}1.6{\mu}g/mL$의 $IC_{50}$ 값을 나타내었다. 이로 미루어 보아, 쪽 꽃 추출물과 두 활성물질의 멜라닌 생합성 저해활성을 확인할 수 있었으며, 쪽 꽃추출물의 미백소재로서의 적용 가능성을 확인할 수 있었다.

• 한국작물학회지
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• 제58권2호
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• pp.177-184
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• 2013

쪽 두 품종, 나주재래종과 나람블루를 사용하여 높은 생엽수량 및 색소함량을 얻기 위한 적정한 이식시기와 수확시기를 알아보고자 수행한 재배시험 결과, 대체적으로 두 품종 모두 비슷한 경향을 나타냈다. 이식시기가 늦을수록 생엽수량은 감소하는 경향을 보였으며, 이식시기 시험구간 다소 차이가 있었지만 니람이나 색소함량은 유의한 차이를 보이지 않았다. 수확시기가 늦어질수록 초장, 분지수, 생엽수량은 증가하는 경향을 보였지만, 니람함량은 8월 20일까지 증가하다가 이후 정체하거나 감소하는 경향을 보였다. 수확시기에 따른 인디고 함량 변화는 8월 5일에 가장 높은 함량을 보였고 이후 감소하는 경향을 보였다. 따라서 쪽 재배에서 높은 생엽수량과 색소함량을 얻기 위해서는 가능한 이르게 이식하는 것이 좋으며 8월 초순경에 수확하는 것이 유리한 것으로 판단되었다.

• 한국작물학회지
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• 제57권3호
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• pp.209-214
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• 2012

나주재래종과 새로이 선발된 나주2호의 지역별 재배시험 결과, 초장은 나주2호가 다소 컸으며, 분지수는 나주재래종이 나주2호 보다 많은 경향이었다. 엽면적은 나주2호가 더 넓었으며, 엽장폭비도 나주2호가 더 크게 나타나 둥근 잎 특성의 안정성을 보였다. 두 계통의 지상부 생중과 건중은 큰 차이가 없었으나, 지상부 전체에 대한 엽의 무게 비율은 나주2호가 더 높아 색소수율면에서 유리하였다. 인디고(indigo) 성분과 니람(泥藍) 함량은 나주2호가 나주재래종 보다 높게 나타났으며 실크로 생엽 이용 염색을 한 결과 청색을 더 많이 나타냈다. 따라서 엽 수량이 높고 색소 품질이 좋은 나주2호가 쪽 재배에 유리한 계통으로 판단되었다.

• Journal of Ginseng Research
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• 제46권5호
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• pp.628-635
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• 2022

Background: Ulcerative colitis (UC) is the large intestine disease that results in chronic inflammation and ulcers in the colon. Rg3-enriched Korean Red Ginseng extract (Rg3-RGE) is known for its pharmacological activities. Persicaria tinctoria (PT) is also used in the treatment of various inflammatory diseases. The aim of this study is to investigate the attenuating effects of Rg3-RGE with PT on oxazolone (OXA)-induced UC in mice. Methods: A total of six groups of mice including control group, OXA (as model group, 1.5%) group, sulfasalazine (75 mg/kg) group, Rg3-RGE (20 mg/kg) group, PT (300 mg/kg) group, and Rg3-RGE (10 mg/kg) with PT (150 mg/kg) group. Data on the colon length, body weight, disease activity index (DAI), histological changes, nitric oxide (NO) assay, Real-time PCR of inflammatory factors, ELISA of inflammatory factors, Western blot, and flow cytometry analysis were obtained. Results: Overall, the combination treatment of Rg3-RGE and PT significantly improved the colon length and body weight and decreased the DAI in mice compared with the treatment with OXA. Additionally, the histological injury was also reduced by the combination treatment. Moreover, the NO production level and inflammatory mediators and cytokines were significantly downregulated in the Rg3-RGE with the PT group compared with the model group. Also, NLR family pyrin domain containing 3 (NLRP3) inflammasome and nuclear factor kappa B (NF-𝛋B) were suppressed in the combination treatment group compared with the OXA group. Furthermore, the number of immune cell subtypes of CD4⁺ T-helper cells, CD19⁺ B-cells, and CD4⁺ and CD25⁺ regulatory T-cells (Tregs) was improved in the Rg3-RGE with the PT group compared with the OXA group. Conclusion: Overall, the mixture of Rg3-RGE and PT is an effective therapeutic treatment for UC.

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.899-907
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• 2018

The methanol extracts of flowers obtained from 67 plant species were screened for their inhibitory activity on aldose reductase (AR). Alnus japonica, Aster spathulifolius, Chionanthus retusus, Morus bombycis, Crysanthemum boreale, Persicaria tinctoria, Platycarya strobilacea, and Serratula coronata var. insularis exhibited potent aldose reductase inhibitory (ARI) activity. HPLC-UV analysis of quercetin, an AR inhibitory flavonoid, was performed on extracts showing strong ARI activity. Quercetin was detected in C. retusus, C. boreale, P. tinctoria, and S. coronata var. insularis at concentrations of 1.33, 1.56, 0.82, and $3.37mg\;g^{-1}$ extract, respectively, indicating that quercetin contributed to the ARI activity of these extracts. In the samples in which quercetin was absent, other compounds may be responsible for their potent ARI activity. These results serve as a basis for further studies regarding the bioactive components responsible for the inhibitory effects of various flower extracts on AR activity.