• 한국식품과학회지
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• 제44권1호
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• pp.8-13
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• 2012

자외선 조사는 들깨와 들기름에서 지방질 산화를 유발하였으며 들기름에 비해 들깨의 산화안정성이, 볶은 들깨에 비해 생 들깨의 유지 산화안정성이 우수하였다. 자외선 조사 중 들깨와 들기름에 존재하는 토코페롤과 폴리페놀 화합물은 분해되었으며 분해 속도는 들기름보다 들깨에서, 볶은 들깨보다 생 들깨에서 높았다. 또한 자외선 조사 중 들깨에 비해 들기름이 산화방지제 농도에 더욱 민감하게 영향을 받았으며, 토코페롤에 비해 폴리페놀 화합물이 들깨 및 들기름의 산화방지에 높은 기여도를 보였다.

• Preventive Nutrition and Food Science
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• 제10권2호
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• pp.151-155
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• 2005

This study was intended to investigate the effects of regular swimming exercise and vitamin C supplementation on the antioxidant system following exercise stress. For the swimming exercise experiment, a swimming adaptation exercise of 1 week was given to a group of 6-week-old mice. Following this, a swimming exercise for 8 weeks was conducted. The experimental group was divided into 3: a control group (C), a swimming exercise trained group (T), and a group of swimming + vitamin C supplementation (TC: vitamin supplementation: 1.3 mg/l00 g diet). After the swimming exercise, these group were further divided into those that had received the exercise stress for 2 hours and those that had not experienced exercise stress group. Then, the activities of the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) concentrations were measured. There was a lower weight increase in the T and TC groups than in the C group, and there was no significant difference between T and TC group. When exercise stress was not experienced, the activity of SOD was significantly increased in the TC group than in the T group, but there was no significant difference between C and T groups. The groups that had experienced a 2-hour exercise stress showed the SOD activity levels according to the following order, C < T < TC, with a significant difference between the three groups (p<0.05). There was no difference in MDA concentration amongst the experimental groups in non-exercise stress group. As well, there was no differences in MDA concentration between the C group and T group in the 2 hour exercise stress group. However, the TC group showed a MDA concentration level significantly lower than that of the T group. A significant increase in MDA concentration was observed in C group, when exercise stress was provided with no significant difference in the T and TC groups. As a result, regular exercise and vitamin C supplementation can be considered important in controlling the formation of lipid peroxides in exercise stress.

• Journal of Nutrition and Health
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• 제33권5호
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• pp.517-523
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• 2000

The effect of dietary zinc deficiency and age on lipid peroxide level was investigaed in rats. Zinc level in serum and liver were also measured. Fifty Sprague-Dawly male rats aging 8 months(older rats) and 2 months(younger rats) were used as experimental animal. Zinc deficient diet(1.1ppm) and normal zinc diet(36.5ppm) were used as experimental diets. Rats in each age group were divided into zinc deficient(ZnDF), zinc pair-fed(ZnPF) and zinc ad-libitum(ZnAL) to remove the variances of food intake. After 4 weeks of experimetal period, rats were sacrificed. Thiobarbituric acid reactive substance(TBARS) levels in plasma and liver, lipofuscin and conjugated diene levels in liver were measured as lipid peroxide index. Food intakes of all groups were not different because zinc deficiency did not reduce food intake in ZnDF group. Younger rats gained weight continuously, while older rats lost weight in the begining of experiment and regained afterwards. In older rats, serum zinc level was decreaed while plasma TBARS. level was increased in ZnDF group. In younger rats, plasma TBARS concentration was increased in dietary zinc deficient rats although serum zinc concentration was not reduced. Liver zinc concentration was significantly higher in older rats comparing to younger rats. However, there was no difference among the three dietary groups. Liver TBARS level was not different by age or dietary zinc level. However it was tended to be higher in older rats. However there was no difference by the dietary zinc level. In both age groups, ZnDF group significantly increased plasma TBARS levels, which suggested dietary zinc deficiency could increase lipid peroxidation in part. Significantly higher levels of lipofuscin and conjugated diene in older rats suggested lipid peroxidation was accelerated by aging.

• Journal of Nutrition and Health
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• 제37권3호
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• pp.182-192
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• 2004

Conjugated linoleic acid (CLA) is the mixture of positional and geometric isomers of linoleic acid (LA), which is found abundantly in dairy products and meats. This study was performed to investigate the anticarcinogenic effect of CLA in HepG2 hepatoma cells. HepG2 cell were treated with LA and CLA at the various concentrations of 10, 20, 40, 80 uM each at different incubation times. After each incubation times, cell proliferation, fatty acids incorporation into cell, peroxidation and postaglandin E$_2$ (PGE$_2$) and thromboxane $A_2$ (TXA$_2$) for the eicosanoid metabolism were measured. LA treated HepG2 cells were increased cell growth 6 - 70％ of control whereas CLA increased cell death the half of those in LA group (p 〈 0.001). LA and CLA were incorporated very well into the cellular membranes four times higher than in control according to concentration and longer incubation times. Moreover, LA synthesized significantly arachidonic acids corresponding with LA concentration compared to CLA supplementation. The supplementation with LA increased intracellular lipid peroxides concentration corresponding with LA concentration and five times higher than those in CLA significantly at any incubation times (p 〈 0.001). PGE$_2$ and TXA$_2$ levels were three to twenty times lower in condition of CLA treatments than LA, respectively. Overall, the dietary CLA might change the HepG2 cell growth by the changes of cell composition, production of lipid peroxide. Since CLA have not changed the levels of arachidonic acid of cell membrane, which was sources of eicosanoids, eicosanoid synthesis was not increased in CLA compared to LA. Our results was suggest CLA has a possibility to protect the progress of atherosclerosis because CLA does not produce lipid production and endothelial contraction factors in liver.

• Journal of Nutrition and Health
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• 제41권4호
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• pp.291-298
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• 2008

To study antioxidant role of zinc, the effects of dietary zinc deficiency and vitamin E supplementation on lipid peroxidation were studied. Levels of zinc and vitamin E in blood and liver were also measured. Forty Sprague-Dawley male rats aging 8 weeks old were used as experimental animals. Zinc deficient diet (Zn, 0 ppm), zinc normal diet (Zn,36.5 ppm), and vitamin E supplemented diet (1,000 IU ${\alpha}$-tocopherol/kg of diet) were used as experimental diet. During the first three weeks, rats were divided into zinc normal (ZnN, 8 animals) and zinc deficient (ZnD, 32 animals) group. Eight rats from each group were sacrificed to get blood and liver after 3 weeks of experiment. The remaining 24 zinc deficient rat were then divided into zinc normal (ZnDN), zinc deficient (ZnDD), vitamin E supplemented (ZnDE) diet groups. After another 3 weeks of experiment, all animals were sacrificed as well. Thiobarbituric acid reactive substanc (TBARS) levels in plasma and liver, conjugated diene levels in liver were measured as lipid peroxidation index. There were no significant differences in food intake, body weight gain, and food efficiency ratio among groups. Weights of liver per 100 g body weight were not significantly different. There were no significant differences in Zn levels in serum. Plasma and liver TBARS level, and liver conjugated diene level were significantly lower in ZnDE than in ZnDN or ZnDD, and significantly higher in ZnDD than in ZnDN. Therefore, it seems that lipid peroxidation is accelerated by dietary zinc deficiency and recovered partly by vitamin E supplementation.

• 폴리머
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• 제33권2호
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• pp.131-136
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• 2009

과산화물 가교제의 화학적 구조가 에틸렌 비닐 아세테이트 공중합체(EVA)의 용융 가교 반응에 미치는 영향과 가교 EVA의 물성을 살펴보았다. 한 개의 peroxy group을 갖는 perbutyl peroxide(PBP)가 두 개의 peroxy group을 갖는 2,5 dimethyl 2,5 di(tert-butylperoxyl) hexane (25B40)과 1,1-di(tert-buthylperoxy)-3,3,5-tri-methylcyclohexane(3M40) 보다 효과적으로 EVA 용융 가교 반응을 유발시킴을 확인하였으며 가교 촉진제의 사용에 따라 가교 반응 시간은 단축되나 가교도의 증가는 크지 않음을 알 수 있었다. 가교 EVA는 EVA에 비하여 가교에 의한 망상 구조에 의하여 흐름 특성은 감소하는 반면 기계적 특성이 우수해 짐을 알 수 있었다.

• 한국자원식물학회지
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• 제13권3호
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• pp.171-178
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• 2000

본 실험은 간장기능보호를 위한 식품소재를 검토하기 위하여 수행되었다. 인진쑥, 콩나물, 솔잎추출물은 in vitro 간장 fatty acid synthase 활성을 억제하였다. 한편, 솔잎, 콩나물추출물은 사염화탄소를 처리한 횐쥐의 혈청 GPT및 GOT효소활성의 상승을 억제하였다. 인진쑥, 콩나물추출물은 사염화탄소처리에 의한 흰쥐의 간장 microsome의 과산화지질함량의 상승을 억제하였다. 나아가 콩나물추출물은 사염화탄소에 의해 증가한 흰쥐의 간장콜레스테롤 및 중성지질함량을 현저하게 감소시켰다. 이러한 결과는 인진쑥, 솔잎, 콩나물추출물의 사염화탄소에 의한 간장손상의 보호기능을 보여 준다.

• 대한본초학회지
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• 제32권2호
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• pp.49-56
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• 2017

Objectives : This study investigated the anti-oxidant activities and improving effect of Phellinus linteus and Glycyrrhiza uralensis Extract (PGE) on Atopic Dermatitis. Methods : 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical, Hydrogen peroxides scavenging activities and Superoxide dismutase (SOD)-like activities were used for the measurement of anti-oxidant ability. Cytotoxicity of PGE in Raw 264.7 cell was evaluated by MTT assay. To evaluate the anti-atopic dermatitis effect of PGE, a total of 33 patients with atopic dermatitis were observed trans epidermal water loss, skin moisture content, modified SCORAD index of atopic dermatitis and pruritic degree after applying the PGE for 4 weeks. Results : PGE scavenged DPPH ($IC_{50}=25ppm$) effectively, ABTS and Hydrogenperoxides scavenged similar to BHA. As for the SOD-like activity, it had lower effect than ascorbic acid, but it comparable activities in 500ppm. There was no cytotoxicity at PGE at concentrations of 10,000ppm. In clinical research about PGE on patients with atopic dermatitis, skin condition was improved. After 4 weeks, the application of PGE increased skin moisture content from 19.43 to 31.22. Moreover, it reduced the skin temperature (from 32.5 to 31.9), skin pH (from 5.39 to 5.22), trans epidermal water loss (from 39.03 to 24.46) and pruritus score (from 6.07 to 3.87). In addition, the Modified SCORAD index decreased from 31.28 to 20.3. Conclusions : In conclusion, PGE possesses anti-oxidant and anti-atopic dermatitis activities, thus it could be potentially valuable as anti-atopic dermatitis material.

• 한국식생활문화학회지
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• 제24권6호
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• pp.749-755
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• 2009

We investigated the chemical components of red onion powder dried using the low temperature vacuum method and the inhibitory effects of solvent extracts of the dried red onion powder on the growth of HT-1080 human fibrosarcoma and HT-29 human colon cancer cells and $H_2O_2$-induced oxidative stress. The moisture content of the dried red onion powder was 17.95%, while the vitamin C content was 96 mg/100 g and the total phenols content was 39.1 mg/mL. The inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts of the red onion powder on the growth of HT-1080 and HT-29 cancer cells increased in a dose dependent manner (p<0.05). The inhibitory effect was greater on the growth of HT-29 cells, while the A+M extracts had a higher inhibitory effect than the MeOH extracts. Treatment with the hexane, 85% aq. methanol, butanol and water fractions of the extract led to significant inhibition of the growth of both cancer cell lines (p<0.05). Among the fractions, the hexane and 85% aq. methanol fractions showed a greater inhibitory effect. To determine the protective effect on $H_2O_2$-induced oxidative stress, a DCFH-DA (dichlorodihydrofluorescin diacetate) assay was conducted. All fractions, including the crude extracts of dried red onion, appeared to lead to a significant reduction in the levels of intracellular reactive oxygen species (ROS), and these reductions occurred in a dose dependent fashion (p<0.05). Among the fractions, the 85% methanol fraction showed the greatest protective effect on the production of lipid peroxides.

• KSBB Journal
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• 제10권5호
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• pp.561-567
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• 1995

활성산소 소거 효소인 superoxide dismutase (SOD)가 결핍된 효소 변이주들을 대상으로 하여, 활성산소 유발물질인 paraquat을 배지에 첨가하여 배양하면서 산소 독성을 관찰하였다. 호기 상태에서 는 MnSOD (mitochondria SOD) 결핍 효모는 CuZnSOD (세포질 SOD) 결핍 효모보다 성장이 많 이 저하되었지만, 혐기 상태에서는 이들 SOD 결핍 효모 모두 성장 속도에서 야생 효모와 큰 차이를 보이지 않았다. Paraquat으로 처 리한 결과, 호기 배양 에서 CuZnSOD 결핍 효모는 O.OlmM 이상에서 성 장하지 않음을 알 수 있었다. 따라서 O.OOlmM paraquat을 배지에 첨가한 후 호기적으로 배양하면서 세포내 성분의 변화를 관찰하였더니, CuZnSOD 결핍 효모에셔는 catalase 역가가 떨어진 반면 glutathion peroxidase 역가와 세포막 지질의 과산 화물이 증가하였으며, MnSOD 결핍 효모에서는 catalase 역가와 glutathion peroxide 역가가 모두 조금씩 증가하면서 세포막 지질의 과산화물은 그다지 변화하지 않았다. 이러한 사실로부터 CuZnSOD 가 없는 경우 활성산소 소거계로써 catalase 보다 glutathion peroxidase가 훨씬 활성화되어지지만, 이렇게 활성화된 glutathion peroxidase로는 세포질내 산소 radical을 완벽히 제거하는데에는 다소 불충분 하다는 사실을 알 수 았었다. 한편, 혐기적 배양에서 는 SOD 결핍 효모들의 catalase 역가는 모두 감소 한 반면, glutathion peroxidase 역가는 다소 증가하였고, 또한 세포막 지질의 과산화물은 다소 감소하는 추세를 보였다. 이는 혐기 상태에서 산소 radical 이 소량밖에 생기지 않으므로, 활성화된 glutathion p peroxidase에 의해 어느 정도 극복되어지기 때문인 것으로 사료된다. 두 집단 간에 어느 정도 방향성은 있는 것으로 판단된다.ds for eagerness for lasting life not only in this world but also in the other world., Keun-Kap, Scarf, Pee-Bak, Hung-Kap, Pok-Kap, Yang-Dang-Kap, We-Yo-Kap, Kum-Kap, and caries arms. Lower-level officials wore Pe-Bal, Kun-Mo, gae, won-leung, very small sleve jacket, a long coat reaching up to the knee length, slacks, belt, loin cloth and apron. 5) Children's bind their hair up angle shape and wore a half long jacket raching up to the hip and slacks. 나) Women's wear; 1. hair style and hair dress; 1) High rank women's hair style was very extravaganceful. They made their hair top knot (one, two, or more knots) and decoraed precious stone, pan shape head dress, wheel shape head dress, and flower shape precious stone decorated head dress. 2. Clothes ; 1) High rank ladi's wore Kun-Kyun attached jacket, and jacket sleeves decorated pleats, and pleats decorated long skirt, apron, back apron, knot belt, scarf, this type is the same