• Analytical Science and Technology
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• v.20 no.1
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• pp.1-9
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• 2007

The BOD (biochemical oxygen demand) sensor was fabricated by covering a dissolved oxygen (DO) probe with a microbe-impregnated membrane and a dialysis membrane. Various microorganisms isolated from the soils, water and activated sludge have been evaluated for measuring biochemical oxygen demand (BOD); Bacillus species HN24 and HN93 were selected as they exhibited rapid oxygen consumption and fast recovery. Improved BOD sensor could be prepared by using mixed microbes (Bacillus subtilis, Bacillus sp. HN24 and Bacillus sp. NH93) and silicon rubber gas-permeable membrane for DO probe, and by bubbling 50% $O_2$ ($N_2$ valence) through background buffer solution. This system exhibited excellent analytical performance resulting in good linearity ($r^2=0.9986$) from 0 to 100 mg/L level of BOD.

• Fashion & Textile Research Journal
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• v.25 no.1
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• pp.119-126
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• 2023

This study aimed to determine the effects of relative humidity and fiber properties on the moisture permeability of multilayer systems by measuring water vapor transmission in the overlapping condition of various fabrics. The results confirmed that the property of the fabric in contact with the humid environment affects the moisture permeability. If the layer facing the humid environment is hydrophobic and the layer facing the dry environment is superhydrophobic, water vapor transmission increases by up to 17.8% compared to the opposite conditions. Comparing the correction values of the water vapor transmission reflecting the thickness of the specimen under the multilayer condition showed that permeability was higher when the hydrophilic or hydrophobic layer was facing the humid environment. The opposite was true from the "push-pull" effect of absorption mechanism. In the case of moisture permeability, the more hydrophilic the surface facing the humid environment, the more permeable that water vapor diffuses and passes through. It was concluded that the "pull-push" effect, in which water vapor diffuses widely through the hydrophilic facing a humid environment and then passes through the hydrophobic layer, contributes to the improvement of permeability. Permeability differed according to the multilayer overlapping condition. When the relative humidity was high, the "pull-push" effect was insignificant. This is caused by water droplets absorption after the partial migration of water due to condensation. These results suggest that the overlapping conditions and properties of fabrics should vary depending on heavy sweating or not.

• Journal of Aerospace System Engineering
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• v.17 no.2
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• pp.26-37
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• 2023

Urban air mobility (UAM) is being developed as part of the next-generation aircraft, which could be a viable solution to entrenched problems of urban traffic congestion and environmental pollution. A new airport platform called vertiport as a space where UAM can take off and land vertically is also being introduced. Noise regulations for UAM will be strict due to its operation in a highly populated urban area. Ground effects caused by vertiport can directly affect aerodynamic forces and noise characteristics of UAM. In this study, ground effects of vertiport on aerodynamic loads, vorticity field, and far-field noise were analyzed using Lattice-Boltzmann Method (LBM) simulation and Ffowcs Williams and Hawkings (FW-H) acoustic analogy with a permeable surface method.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1B
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• pp.115-123
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• 2008

In the present study, a three dimensional hydrodynamic characteristics around the fully submerged dual breakwaters with a sand beach has been investigated numerically using a 3-D numerical scheme, which can determine the eddy viscosity with LES turbulence model and is able to consider wave-structure-seabed interaction in 3-dimensional wave field (LES-WASS-3D), recently developed by Hur and Lee (2007). Based on the numerical experiments, strong vortices can be generated fore and aft edges of the structures, and propagate lee sides. Thus relatively large circulation flows are occurred around submerged breakwaters. The 3-D flow hydrodynamic characteristics have been examined by mean flows and mean vortices for various x-y, x-z sections and y-z layers. Wave height distribution and wave set-up around and over submerged breakwaters, and breaking point migration toward shore side is discussed in detail.

• Journal of English Language & Literature
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• v.56 no.5
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• pp.797-821
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• 2010

This paper aims to explore how Millay's love sonnets rewrite the carpe diem tradition in the complicated ways. This paper redirects critical attention away from Millay's individual experience and inner self toward the scene of literary history, suggesting that there may be more historical consciousness in Millay's sentimental and feminine "gesture." Rewriting the carpe diem tradition, Millay's sonnets reveal an awareness of the dependence of the carpe diem poems' discursive logic on the woman's coyness, its inability to accomplish its triumph over woman or time (death) without her posited reluctance. Contrary to Andrew Marvel's "To His Coy Mistress," the speakers of Millay's sonnets could never be accused of the sexual coyness; they are outspoken in their defiance of both death and lovers whose possessiveness resembles death's embrace. Moreover, as Stacy Carson Hubbard points out, by converting female sexual experience from its status as a onetime closural event to repeatable one, hence an opportunity for the general and emotional irritability productive of narrative, Millay seizes for the woman the power of "dilation" in both its sexual and its verbal forms. Furthermore, this paper argues that the woman's sex no longer invites analogies to things secret and sealed, preserved or ruined in Millay's sonnets. The woman's promiscuity implies a rejection of monumentalizing love, as well as a refusal of the fixing inherent in the carpe diem's fearful invocation of the movement of time. Throughout the love sonnets, the speaker's sexualized body produces nothing but ephemera. For Millay, this body spends its powers in hopes of having them, and the force of this spending is a perpetual and willful forgetting, which makes possible the repetition of love's story. Ultimately, Milly disturbs our critical categories by rendering permeable boundaries between modern literature and dead form of classic literature, the female speaker and male speaker.

• Journal of Korean Society on Water Environment
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• v.40 no.3
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• pp.131-139
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• 2024

This study investigated the chemical properties of Organic Soil Amendments (OSAs) made from organic waste. It also assessed the effectiveness of using these OSAs in the soil layer of Green Infrastructure (GI) to reduce stormwater runoff and non-point source pollutants. The goal was to improve the national environmental value through resource recycling and contribute to the circular economy transformation and carbon neutrality of urban GI. The OSAs used in this study consisted of spent coffee grounds and food waste compost. They were found to be nutrient-rich and stable as artificial soils, indicating their potential use in the soil layer of GI facilities. Applying OSAs to bio-retention cells and permeable pavement resulted in a reduction of approximately 11-17% in stormwater runoff and a decrease of about 16-18% in Total Phosphorus (TP) discharge in the target area. Increasing the proportion of food waste compost in the OSAs had a positive impact on reducing stormwater runoff and pollutant emissions. This study highlights the importance of utilizing recycled resources and can serve as a foundation for future research, such as establishing parameters for assessing the effectiveness of GI facilities through experiments. To enable more accurate analysis, it is recommended to conduct studies that consider both the chemical and biological aspects of substance transfer in OSAs.

• Journal of Soil and Groundwater Environment
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• v.29 no.3
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• pp.1-13
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• 2024

This study investigated the hydrogeochemical and microbiological characteristics of freshwater on Ulleung Island, a volcanic island in the Ulleung Basin on the East Coast of Korea. The shallow groundwater (CSW, NRGW) and the surface water (SISW) samples are classified as Na-HCO₃ type, reflecting an alkaline rock type and an oxidizing environment due to the influence of a highly permeable pyroclastic rock layer. In contrast, the deep groundwater sample (DMW) is classified as Ca-HCO₃ type, suggesting the influence of deep-sourced carbon dioxide and reducing conditions. Microbial communities in the water samples are generally dominated by Proteobacteria, with the relative abundance of major genera varying depending on water quality and environmental conditions. Network analysis reveals the ecological characteristics of microbial communities adapted to specific environments. The presence of pathogenic genera in the shallow groundwater suggests potential groundwater contamination, necessitating appropriate management to ensure its use as drinking water or domestic water. The findings of this study provide valuable insights into the ecological characteristics of Ulleung Island's groundwater resources and can inform future groundwater management strategies.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.407-414
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• 2024

Phosphorus is an essential but non-renewable nutrient resource critical for agriculture. Luxury phosphorus uptake allows microalgae to synthesize polyphosphate and accumulate phosphorus, but, depending on the strain of algae, polyphosphate may be degraded within 4 hours of accumulation. We studied the recovery of phosphorus from wastewater through luxury uptake by an engineered strain of Synechocystis sp. with inhibited polyphosphate degradation and the effect of this engineered Synechocystis biomass on lettuce growth. First, a strain (∆phoU) lacking the phoU gene, which encodes a negative regulator of environmental phosphate concentrations, was generated to inhibit polyphosphate degradation in cells. Polyphosphate concentrations in the phoU knock-out strain were maintained for 24 h and then decreased slowly. In contrast, polyphosphate concentrations in the wild-type strain increased up to 4 h and then decreased rapidly. In addition, polyphosphate concentration in the phoU knockout strain cultured in semi-permeable membrane bioreactors with artificial wastewater medium was 2.5 times higher than that in the wild type and decreased to only 16% after 48 h. The biomass of lettuce treated with the phoU knockout strain (0.157 mg P/m²) was 38% higher than that of the lettuce treated with the control group. These results indicate that treating lettuce with this microalgal biomass can be beneficial to crop growth. These results suggest that the use of polyphosphate-accumulating microalgae as biofertilizers may alleviate the effects of a diminishing phosphorous supply. These findings can be used as a basis for additional genetic engineering to increase intracellular polyphosphate levels.

• Development and Reproduction
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• v.11 no.2
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• pp.111-119
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• 2007

Differentiation of cells can be induced through modulation of endogenous regulators using exogenous factors. Useful transfection systems to transport a specific exogenous regulator into cell have been tried but still there are many obstacles to overcome. In this study, we examined the transfection efficiency of cell permeable peptides (CPPs) in mouse embryonic stem cell under the various conditions. To identify the CPP-mediated translocation of a protein, we employed recombinant CPP-enhanced green fluorescent protein (EGFP). Viability of R1 cells was different between experimental groups depending on the kind of CPP and the concentration of CPP-EGFP. Translocation of CPP-EGFPs into the R1 cells was not detected until 30 min after CPP-EGFPs treatment in all groups. After 1 hr, translocation of pEP-1-EGFP-N was detected, but it could not be detected in the other group. Transfection of pEP-1EGFP-N was independent on its concentration. The time course did not show saturation even after 24 hr in pEP-1-EGFP-N. These results showed that the permeability depended on the kind of CPP and the location of His-tag in the case of examined CPPs, and did not need biological energy. On summary, the efficiency of transfection of CPP-EGFP depends on the CPP sequences but the culture time is not a key factor in transfection for the mouse embryonic stem cell. For the future studies to improve the efficiency of translocation of protein into embryonic stem cells, it is needed to develop modified CPP or mediator. The studies would be very useful to induce the differentiation of embryonic stem cells.

• Journal of Bio-Environment Control
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• v.20 no.3
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• pp.221-226
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• 2011

The study was carried out to investigate the effect of MA packaging materials on quality and storability of tomato in long distance export condition. We found that the fresh weight loss in perforated film was higher than other breathable films. The fresh weight of tomato (cv 'Madison') packaged with breathable films, such as 5,000 cc, 10,000 cc, $20,000cc/m^2{\cdot}day{\cdot}atm$ oxygen permeable films, reduced less than 0.6%, but perforated film that already being used for packaging horticultural crops showed 1.4% fresh weight loss during $5^{\circ}C$ storage for 20 days and then $20^{\circ}C$ storage for last 5 days with 85% relative humidity. The carbon dioxide and oxygen concentration in tomato packages showed proper level for MA storage in $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ breathable film treatment at $5^{\circ}C$ storage. Although at $20^{\circ}C$ storage, the carbon dioxide concentration increased sharply, the oxygen concentration decreased remarkably, the change of these gases concentration was the lowest in 20,000 cc film treatment. The ethylene concentration was sharply increased at $20^{\circ}C$ from $21^{st}$ day to $25^{th}$ day after $5^{\circ}C$ storage for 20 day, and the concentration was lower in 20,000 cc film treatment among the breathable film treatments. Until 20 days, at $5^{\circ}C$ storage all treatments did not exceed the marketability. However, the last 5 days during at $20^{\circ}C$, the fruit appeared fungal rots and the quality rapidly decreased. The $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ permeability treated tomato performed higher firmness (9.56 N), vitamin C (16.31 mg/100 gFW), and soluble solids ($7^{\circ}Brix$) than other breathable films at final storage day. The results suggest that the $20,000cc/m^2{\cdot}day{\cdot}atm$ $O_2$ permeable film treatment of tomato (cv 'Madison') performed the highest quality and storability of tomato for long distance exporting.