• Applied Biological Chemistry
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• v.35 no.3
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• pp.146-151
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• 1992

To investigate changes in fatty acid and lipid composition of maturing perilla (Perilla frutescens var. japonica Hara) seeds, Suweon 10 and Jeju varieties were subjected to lipid analysis. The results were summarized as follows; As perilla seeds matured, content of glycolipid and phospholipid decreased. Glycolipid and phospholipid of mature seed were 25.4% and 4.5% of total lipids in Suweon 10 and those from Jeju were 44.5%, 4.0%, respectively. Cerebroside, galatosyl diglyceride and monogalatosyl diglyceride were major constitutents of ether-extractable glycolipids in developing perilla seeds. Monogalatosyl diglyceride, the richest constitutent in the early stage of seed development, decreased rapidly as seeds matured. In ether-extractable glycolipid of mature Suweon 10 seeds, content of linolenic acid was 51.1% which was higher than 19.4% of oleic acid of total acids. However, in Jeju variety, content of oleic acid was 31% which was higher than 18.6% of linolenic acid. Phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol and lysophosphatidyl inositol were major constituents of phospholipids. Content of phosphatidyl glycerol in developing seeds showed irregular changes in Jeju but in Suweon 10, it decreased after rapid increase in the early stage of seed development. Olieic acid ranges $28.7{\sim}35.2%$, linolenic acid $8.2{\sim}11.2%$, linoleic acid $16.4{\sim}32.5%$ and Palmitic acid $22.7{\sim}29.9%$ of total fatty acids in methanol-ertractible phospholipid of mature perilla seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.158-163
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• 2018

Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.

• Korean journal of applied entomology
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• v.35 no.1
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• pp.15-17
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• 1996

Acanthopsyche nigraplaga Wileman, Psychidae is reported for the first time from Korea with a brief redescription and illustration of its male genitalia. Their food plants, Gomphrena globosa L., Rhubus parvifolius L. var. triphyllus N., Perilla frutescens B. var. acuta K., Menispermum dahuricum Dc., and Chenopodium album L. are also newly reported.

• Journal of the Korean Applied Science and Technology
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• v.5 no.1
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• pp.13-23
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• 1988

Contents of total lipids, neutral lipids, glycolipids and phospholipids of seed oils of 16 species of the Labiatae family were determined and their fatty acid compositions were analyzed by gas-liquid chromatography. The results were summarized as follows. 1) Lipid contents of seeds were shown to be 40.6％ in Perilla frutescens Britton var. japonica, 32.2％ in P. frutescens britton var. acuta, 31.9％ in lsodon japonicus, 32.7% in l. inflexus, 48.3％ in l. serra, 35.1％ in Mosls dianthera, 38.2％ in M. punctulata, 33.4％ in Nepeta cataria, 26.3％ in Agastache rugosa, 30.9％ in Eisholtzia ciliata, 18.9％ in Salvia splendens, 23.9％ in Lycopus maackianus, 49.5％ in Clinopodium chinense var. parviflorum, 30.9％ in Ametystea caerulea, 33.1％ in Leonurus sibircus and 34.3％ in Scutellaria basicalensis. 2) Contents of neutral lipids, glycolipids and phospholipids from the seed oils amounted to 98.6％, 0.7％, 0.8％ in P. frutescens Britton var. japonica; 95.5％, 1.3％, 3.1％ in P. frutescens Britton var. acuta; 95.1％, 1.8％, 3.1％ in l. japoincus; 91.4％, 3.5％, 5.1％ in l. inflexus; 96.8％, 0.7％, 2.5％ in l, serra; 96.0％, 1.8％, 2.2％ in Mosla dianthera; 94.7％, 2.0％, 3.3％ in M. punctulata; 90.1％, 2.4％, 7.5％ in Nepeta cataria; 90.1％, 3.4％, 6.5％ in Agastache rugosa; 86.3％, 3.3％, 10.4％ in Elsholtzia ciliata; 94.3％, 1.5％, 4.3％ in Salvia splendens; 87.2％, 2.9％, 9.0％ in Lycopus maackianus; 87.0％, 1.5％, 11.5％ in Clinopodium chinense var. parviflorum; 91.8％, 1.6％, 6.6％; 95.5％, 0.4％, 4.1％ in Leonurus sibricus; 89.0％, 1.4％, 9.6％ in Scutellaria baicalensis. 3) Total lipids revealed the predominace of unsaturated fatty acids (82.0-94.5％) and larger variations were found in the composition of ${\alpha}-linolenic$ acid (0.4-67.9％) and linoleic acid (11.2-82.9％). High level of ${\alpha}-linoenic$ acid was present in P. frutescens Britton var. japonica (67.9％), P. frutescens Britton var, acuta (66.0％), lsodon japonicus (65.2％), l. inflexus (59.0％), l. serra (57.3％), Mosla dianthera (60.9％), Nepeta cataria (58.3％), Agastache rugosa (58.5％) and Elsholtzia ciliata (46.2％), and followed by linoleic acid (11.2-32.1％) and oleic acid (9.3-12.2％). However, linoleic acid was the most predominant component in the total lipids of Clinopodium chinense var. parviflorum (62.4％), Ametystea caerules (82.9％), Leonurus sibricus (60.9％) and Scutellaria baicalensis (63.4％), with very small amounts of ${\alpha}-linolenic$ acid (0.4-3.1％). The total lipids of Salvia splendens, Lycopus maackianus and Mosla punctulata also contained linoleic acid of 31.3％, 48.8％ and 53.4％, with a considerable amount of ${\alpha}-linolenic$ acid of 34.5％ 27.0％ and 16.7％. Palmitic acid was the major saturated fatty acid in all the oils investigated (4.1-14.2％). 4) Fatty acid profiles of neutral lipids bore a close resemblance to those of total lipids in all the seed oils, but different from those of glycolipids and phospholipids. Fatty acid composition pattern of glycolipids and phospholipids showed a considerably increased level of saturated fatty acids (19.0-66.8％, 17.8-35.2％) mainly composed of palmitic acid and stearic acid, and a noticeable low level of unsaturated fatty acids (41.2-80.9％, 64.7-82.1％) which was ascribed to the decrease in ${\alpha}-linolenic$ acid of high ${\alpha}-linolenic$ acid seed oils, and in linoleic acid of high linoleic seed oils, compared to that of total lipids and neutral lipids.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.222-224
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• 2011

The effects of seawater on growth of lettuce(Lactuca sativa L.), leaf perilla(Perilla frutescens var. japonica Hara), red pepper(Capsicum annuum L.) and cucumber(Cucumis sativus L.) seedlings were investigated in the glass greenhouse. These effects were studied on seedlings, and diluted seawater (1%, 5%, 10%, 20%, 50%, 100% v/v) was sprayed enough on leaves. The tested four vegetable crops have well grown up to 10% diluted seawater, but the tested vegetable crops were damaged from increasing salt levels. Of these, lettuce was provided salt-tolerant vegetable crop and red pepper was considered salt-sensitive vegetable crop. The salt tolerance of vegetable crops is different between crops and complicated because of additional detrimental effects caused by accumulated ions or specific ion toxicities in their leaves. These results show that agricultural use of seawater may be benefit crop cultivation in organic farming system as well as in conventional farming system.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.317-325
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• 2006

According to the preceding survey on insect pests of the green perilla, Perilla frutescens var. japonica HARA, The major pests were Aphis egomae Shinji, Pyrausta panopealis (Walker), Tetranychus urticae Koch, Polyphagotarsonemus lotus Banks, Tetranychus kanzawai Kishida at Guemsan, Chungnam, 2004. Aphis egomae causes nearly 100% injury of the green perilla in uncontrolled green houses. A field study was conducted to estimate economic injury levels (EILs) and control thresholds (CTs) for A. egomae injuring green perilla in green houses. Different densities of A. egomae ranged from 1 to 80 aphids per 100 plants in early inoculation. The mean injurying rate of plant was 2.4% to 40.5% at the end of June at differently inoculated levels. The economic loss time calculated by the ratio of cost managing aphid to market price (C/V) (C: cost managing aphid, V: Market price) in early season (from May to 13. June) was 5.8% and in peak season (from 13. June to 30. June) was 9.3%. Economic injury level in early and peak season was 5.3 aphids per plant and economic injury levels in peak season were 0.6 aphids per plant and 7.6% injured rate of plant. The control thresholds calculated by 80% level of economic injury level in peak season were 0.5aphids per plant and 6.1% injury rate of plant, respectively.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.71-76
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• 2014

There have not been many studies conducted on the seedling production, especially in plug trays, of traditional medicinal plant species. In an effort to establish guide lines for seedling production, this study investigated the effect of plug cell size on the growth and development of plug seedling of three medicinal plant species. Seeds were sown in either 128, 200, or 288-cell plug trays, containing a commercial medium. Growth and development of individual seedling was generally promoted with increasing size of a plug cell in all of the three species. The greatest biomass of the seedlings gained in a plug tray was obtained in the 288-cell trays in Perilla frutescens var. acuta Kudo and Sophora tonkinensis, and the 200-cell trays in Angelica gigas Nakai. Overall growth and development of the shoot and root of a single seedling of Perilla frutescens var. acuta Kudo, except total chlorophyll and anthocyanin contents, was the greatest in the 128-cell tray. However, length of the longest root, length, width and area of the leaf, internode length, root fresh weight, and root ball formation in the 200- and 288-cell trays were not significantly different each other. In Sophora tonkinensis, although length of the longest root, stem diameter, leaf width, leaf area, shoot fresh weight, and root ball formation were not significantly different among the treatments, length of the longest root and root ball formation of a single seedling were the greatest in the 128-cell tray. Overall shoot and root growth, except total chlorophyll content, of a single seedling of Angelica gigas Nakai was the greatest in the 128-cell tray. Based on the total biomass, it is concluded that 288-cell trays are recommended for production of plug seedlings of medicinal plant species P. frutescens var. acuta Kudo and S. tonkinensis. In A. gigas Nakai, it would be more economical to use the 200-cell trays than 128-cell trays due to total biomass.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.1
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• pp.85-88
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• 2015

This study attempted to establish an HPLC analysis method for determination of marker compounds as a part of material standardization for the development of health functional food materials from Perilla frutescens Britton var. acuta Kudo. The quantitative determination method of rosmarinic acid as a marker compound of P. frutescens Britton var. acuta Kudo extract (PFE) was optimized by HPLC analysis using a C18 column ($4.6{\times}150mm$, $5{\mu}m$) with 0.1% acetic acid as the elution gradient and methanol as the mobile phase at a flow rate of 1 mL/min and detection wavelength of 280 nm. The HPLC/UV method was applied successfully to quantification of the marker compound in PFE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9995, and the limit of detection and limit of quantitation were $0.36{\mu}g/mL$ and $1.2{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 3.21% and 1.43%, respectively. Recovery rate test at rosmarinic acid concentrations of 12.5, 25 and $50{\mu}g/mL$ scored between 97.04~98.98% with RSD values from 0.25~1.97%. These results indicate that the established HPLC method is very useful for the determination of marker compound in PFE to develop a health functional material.

• Journal of Life Science
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• v.27 no.5
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• pp.509-516
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• 2017

Perilla frutescens (L.) Britton var. sprouts (PFS) is a plant of the labiatae family. The purpose of this work was to assess the preventive effects of PFS ethanolic extracts (PFSEs) on cytokine-induced ${\beta}$-cell damage. Cytokines, which are released by the infiltration of inflammatory cells around the pancreatic islets, are involved in the pathogenesis of type 1 diabetes mellitus. The combination of interleukin-$1{\beta}$ (IL-1), interferon-${\gamma}$ (IFN-${\gamma}$), and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) induced formation of reactive oxygen species (ROS). Accumulation of intracellular ROS led to ${\beta}$-cell dysfunction and apoptosis. PFSEs possess antioxidant activity and thus lead to downregulation of ROS generation. Cytokines decrease cell viability, stimulate the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and induce the production of nitric oxide (NO). PFSEs prevented cytokine-induced cell viability in a dose-dependent manner. Incubation with PFSE resulted in significant reduction in cytokine-induced NO production that correlated with reduced levels of the iNOS and COX-2 protein expression. Furthermore, PFSE significantly decreased the activation of nuclear factor ${\kappa}B$ (NF-${\kappa}B$) by inhibition of $I{\kappa}B{\alpha}$ phosphorylation in RINm5F cells. In summary, our results suggest that the protective effects of PFSE might serve to counteract cytokine-induced ${\beta}$-cell destruction. Findings indicate that consumption of Perilla frutescens (L.) Britton var. sprouts alleviates hyperglycemia-mediated oxidative stress and pro-inflammatory cytokine-induced ${\beta}$-cell damage and thus has beneficial anti-diabetic effects.

• YAKHAK HOEJI
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• v.48 no.6
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• pp.384-390
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• 2004

Polyphenol oxidase-catalyzed oxidation of substrates (t-butylcatechol, 4-methylcatechol, chlorogenic acid, caffeic acid and pyrocatechol) were performed in the Ph range 4~8. Co ncentrations of substrate's major oxidation products were monitored by high performance liquid chromatograph. The nature and amounts of products formed were highly pH dependent. They also were ifluenced by kinds of substrates. Major oxidation product of 4-methylcatechol appeared the maxium value at pH 5, them of chlorogenic acid, caffeic acid and pyrocatechol at pH 6.0 and that of t-butylcatechol at pH 5~7. Time-dependent PPO activity was determined at $4^{\circ}C\;and\;30^{\circ}C$. PPO extracted by phosphate buffer containing triton X-114 (t-PPO) was more stable than PPO by phosphate buffer (b-PPO). The result of electrophoresis, at first PPO was showed only a band at 48 kd. After 1~3 days a partial degrade band was appeared in b-PPO and three partial degrade bands in t-PPO. No activity band was appeared in PPOs at $30^{\circ}C$ and b-PPO at $4^{\circ}C$ after 4 days. And a band (37 kDa) in t-PPO was remained finally and disappered. PPO from Perillae leaves has two activity bands at 48 and 37 kDa in previous paper. It was supposed that PPO in the leaves of Perilla frutescens was a protein having one molecular weight as 48 kDa. And 37 kDa protein, relatively proteolysis-resistant, was a proteolyzed form of a major form.