• ALGAE
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• 제20권1호
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• pp.25-30
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• 2005

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

• 미생물학회지
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• 제29권6호
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• pp.361-370
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• 1991

Physical and chemical environmental factors influencing on the outbreak of freshwater red tide by Peridinium bipes (dinoflagellate) in Soyang Reservoir were studied. Red tide occured in the site of inflowing of tributary streams annually, but the extent and severity of red tide varied from year to year. Several environmental factors such as water level, nutrient releasing from sediment, cyst resuspension, and concentrations of $Ca^{2+}$ and $Mg^{2+}$were studiedin relation to development, extent, and duration of red tide. In June of 1989 and 1991, the red tides of Peridinium bipes were very severe, and these red tides coincided with notable and rapid drawdown of lake water in late spring. Nutrient releasing and cyst resuspension by turbulence during drawdown were suggested as main causes of red tide. The quanity of nutrient releasing from sediment and hydrometeorological factors such as run-off and wind may determine the extent and duration of red tide.

• 미생물학회지
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• 제29권6호
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• pp.371-379
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• 1991

Effects of freshwater red tide of Peridinium bipes (dinoflagellate) in August of 1991 on the carbon flux through the microbial loop were studied in Soyang Reservoir. Chlorophyll a and primary production, bacterial production in red tide area were 22, 18 and 400 times higher, respectively, than other sites. Phytoplandton biomass comprised 97% and 20% of food source of zooplankton grazing within and without red tide, respectively. The percent bacterial production supported by phytoplankton exudate was 14% within red tide and >100% without red tide. In laboratory experiments, more than 85% organic carbon of Peridinium biomass was released or degraded by heterotrophic bacteria within 14 days. As results of red tide of Peridinium with sudden influx of organic carbon in water column, the main food source of zooplankton and dependency of bacteria on phytoplankton exudate were changed. Therefore, the relative importance of microbial loop to grazing food web was changed.

• ALGAE
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• 제21권4호
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• pp.433-443
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• 2006

This study was performed to understand the morphological and ecological characteristics of Peridinium bipes var. occultatum Lindem., which is the organism responsible for freshwater red tides in Sangsa dam and Miryang dam reservoirs. The samples were collected from April to July 2004 when the freshwater red tides occurred. In the study, we were able to differentiate P. bipes var. occultatum from P. bipes, a closely related species, by its smaller antapical horn size. In Miryang dam reservoir the red tide occurred only within the area of the upstream, but it was well developed in all of the water area in Sangsa dam reservoir. In 2004 average LTSI (Lake Trophic Status Index; Yang and Dickman, 1993) of Miryang dam reservoir was 3.53 of mesotrophic state and Sangsa dam reservoir was 8.59 of eutrophic state. It was determined, through culture experiments under various conditions that vitamins, trace elements, phosphorus and nitrogen were important contributing factors to the growth of P. bipes var. occultatum. A rapid toxic effect of P. bipes var. occultatum on aquatic organisms such of Daphnia magna and Oryzias latipes was not identified in this study.

• 생태와환경
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• 제38권spc호
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• pp.1-7
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• 2005

To resolve some of the most pressing uncertainties of Peridinium identity, morphological characteristics of Korean Peridinium were examined using light and scanning electron microscopy. The Peridinium samples were collected from three different regions of Juam, Sang-sa and Togyo Reservoirs, when seasonal blooms occurred. Formula of the epithecal plate was recorded with 7 precingular, 3 intercalary, and 4 apical plates (4', 3a, 7"). An apical pore, 3 ${\sim}$ 5 ${\mu}m$ in size, was apparently present. The cingulum was easily observed under light microscope, and was considerably offset by about 15 ${\mu}m$ (2 ${\sim}$ 3 times per cingulum width). The sulcus was straight longitudinally and widened apparently towards the antapex. None of spine was found on the surface of the thecal plates on scanning electron micrographs. The average body length was 50.4 ${\mu}m$ with a range of 29 ${\sim}$ 63 ${\mu}m$. The geometric dimension, as designated to the body length:width ratio, was found from calculation to be 1.12 with a range of 1.00 ${\sim}$ 1.35, therefore, the cell was shown slightly elongated. Based on their morphology, the causative organisms of red tides in three different Korean waters were identified as P. bipes f. occultatum, which was reported for the first time in Korea.

• 생태와환경
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• 제38권2호통권112호
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• pp.249-260
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• 2005

전남-광주권의 주요 상수원의 하나인 주암호의 2개 정점 (취수탑, 문덕교)에서 2003년 1월부터 12월까지 매월1회씩 환경요인과 식물플랑크톤 군집의 변동을 조사하였다. 조사기간 동안 두 정점의 환경요인은 큰 차이를 보이지 않았으나 식물플랑크톤 출현 및 종 조성은 뚜렷한 차이를 나타냈다. 식물플랑크톤은 6분류군 중 녹조류 (41.8%), 규조류(35.8%)가 가장 높은 종 출현을 보였으며, 와편모조류 (46.3%), 남조류 (27.6%)가 가장 높은 현존량을 나타냈다. 취수탑에서는 저온기를 중심으로 주로 Peridinium bipes, 문덕교에서는 고온기를 중심으로 Microcystis aeruginosa가 각각 우점하였으며, 수온 및 조사 정점에 대한 지표성이 높았다. CCA 분석에 따르면, P, bjpes은 DO와 COD에 의해, M. aeruginosa는 수온이나 총질소에 크게 영향을 받는 것으로 나타났다. 따라서 주암호의 식물플랑크톤 군집은 1차적으로 수온에 의해 크게 지배되며, 호수내 연간 1차 생산에 있어 두 우점종 P, bjpes, M. aeruginosa가 가장 크게 기여하는 것으로 나타났다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2004년도 가을 학술발표회지 제13권(제2호)
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• pp.161-162
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• 2004

주암호와 상사호의 담수와편모조 P. bipes 휴면포자의 환경조건에 따른 발아특성을 밝하기 위하여, 매월 채집된 휴면포자를 다양한 수온, 광조건, 영양염, pH 등에서 발아실험을 실시하였다. 실험결과, P. bipes 휴면포자는 수온 $20^{\circ}C$, 광도 $20{\mu}E/m^2/s$, 풍부한 영양원, pH 6-8 등이 최적발아조건으로 밝혀졌다. 특히, 환경요인 중 수온과 광조건은 휴면포자의 발아에 상대적으로 좀더 큰 영향을 주는 것으로 판단된다.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2004년도 학술대회
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• pp.46-46
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• 2004

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2004년도 학술대회
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• pp.48-48
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• 2004

• 환경생물
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• 제23권4호
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• pp.323-327
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• 2005

Two morphologically different species of Peridinium were frequently observed in natural samples collected monthly from Togyo Reservoir. One was previously identified as P. bipes Stein f. occultatum (Lindem.) Lef. (Ki and Han 2005), the other was remained uncertainly so far. We resolved their identity, based on the morphological observations with scanning electron microscopy. The formula of epithecal plate was recorded with 7 precingular, 3 intercalary and 4 apical plates (4', 3a, 7'). An apical pore was apparently present. The conspicuous morphology of the Peridinium was three spines on the hypothecal plates. The average body length and width were $33.6\;{\mu}m$ with a range of $26\~45{\mu}m$, and 25.7 with a range of $19\~31{\mu}m$, respectively; the cell was, therefore, shown slightly elongated. Based on their morphological characteristics, the causative organism was identified as P. aciculiferum (Lemm.), which was reported for the first time in Korean freshwater.