• Title/Summary/Keyword: Pepper bacterial spot

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Near-Isogenic Lines for Genes Conferring Hypersensitive Resistance to Bacterial Spot in Chili Pepper

  • Kim, Byung-Soo;Kim, Young-Chun;Shin, Kwang-Sik;Kim, Jeong-Hoon
    • The Plant Pathology Journal
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    • v.23 no.3
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    • pp.155-160
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    • 2007
  • In order to develop chili pepper bacterial spot resistant cultivars and near-isogenic lines (NILs) to prompt the molecular mapping of the resistance gene, we have run backcross breeding program since 1994. Two resistance genes against Xanthomonas axonopodis pv. vesicatoria Bs2 from Fla. XVR 3-25 and Bs3 from our breeding line 25-11-3-2, were introduced into a land race, Chilseongcho (abbreviated to Chilseong hereafter) with good fruit guality. We report here the testing of $BC_4F_3\;to\;BC_4F_5$. We found that $BC_4F_5$ lines of the crosses were homozygous with respect to the respective genes of introduction. The lines, in which Bs2 gene was introduced, were hypersensitively resistant to both race 1 and race 3 of X. axonopodis pv. vesicatoria, whereas, those in which Bs3 was introduced were resistant to race 1.

Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers (고추 세균성점무늬병 저항성 유전자원과 그 주요 특성)

  • Byeon, Si-Eun;Abebe, Alebel Mekuriaw;Jegal, Yoon-Hyuk;Wai, Khin Pa Pa;Siddique, Muhammad Irfan;Mo, Hwang-Sung;Yoo, Hee Ju;Jang, Kil-Su;Hwang, Ji-Eun;Jeon, Su-Gyeong;Lee, Su-Heon;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.779-789
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    • 2016
  • A total of 33 accessions of pepper (Capsicum spp.), including previously reported and newly discovered sources of resistance to bacterial spot caused by Xanthomonas euvesicatoria, were evaluated for their resistance to bacterial spot. The selected accessions were then grown and their horticultural characteristics were recorded. In a test for hypersensitive resistance (HR) to four races (P1, P3, P7, P8) of the pathogen found in Korea, KC00939 and Chilbok No.2, which carry the Bs2 gene, exhibited a hypersensitive response to all four races, as expected. Chilbok No.3, which carries the Bs3 gene, showed a hypersensitive reaction to race 1 and 7, as expected. KC00939 exhibited a high ASTA color value and tolerance to multiple infections from a viral complex of Cucumber mosaic virus (CMV) and Broad bean wilt virus (BBWV). Thus, this accession represents a promising genetic resource for breeding cultivars with multiple disease resistance and strong red coloration. KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127, KC01704, and KC00995 did not exhibit hypersensitivity but showed a high level of general resistance when evaluated by spray inoculation. KC01617, KC01760, KC01779, KC01137, KC01704, and KC01777 are newly identified sources of resistance to bacterial spot. The previously and newly identified sources of resistance to bacterial spot evaluated in this study, including information about their resistance to CMV and BBWV complex in the field, the contents of pungent and sweet taste components, and the color values of dry fruits, will be useful for breeding pepper cultivars with resistance to bacterial spot.

Selection in $F_3$ and $F_4$ Generations of a Cross for Breeding Genic Male Sterile Pepper Lines Resistant to Phytophthora Blight and Bacterial Spot (역병과 세균성점무늬병에 복합저항성인 핵유전형 웅성불임성 고추 계통 육성을 위한 교배의 $F_3-F_4$세대 선발)

  • Park, Dong-Keun;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.28
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    • pp.17-23
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    • 2010
  • Selection procedures for breeding genic male sterile lines for resistance to both Phytophthora blight caused by Phytophthora capsici and bacterial spot caused by Xanthomonas euvesicatoria were executed to $F_3$ and $F_4$ generations derived from a cross between a Phytophthora resistant genic male sterile (GMS) breeding line and a bacterial spot and Phytophthora resistant breeding line. Resistance to P. capsici was originally introduced from KC294(CM334) and KC263(AC2258), the well-known sources of resistance to P. capsici. Resistance to bacterial spot was introduced from KC47(PI244670). GMS lines with high resistance to P. capsici were obtained and the selected lines are expected to be quantitatively resistant also to bacterial spot.

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Assessment of Root-Associated Paenibacillus polymyxa Groups on Growth Promotion and Induced Systemic Resistance in Pepper

  • Phi, Quyet-Tien;Park, Yu-Mi;Seul, Keyung-Jo;Ryu, Choong-Min;Park, Seung-Hwan;Kim, Jong-Guk;Ghim, Sa-Youl
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1605-1613
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    • 2010
  • Twenty-nine P. polymyxa strains isolated from rhizospheres of various crops were clustered into five genotypic groups on the basis of BOX-PCR analysis. The characteristics of several plant growth-promoting factors among the isolates revealed the distinct attributes in each allocated group. Under gnotobiotic conditions, inoculation of pepper roots with P. polymyxa isolates significantly increased the biomass in 17 of total 29 treated plants with untreated plants. Experiments on induced systemic resistance (ISR) against bacterial spot pathogen Xanthomonas axonopodis pv. vesicatoria in pepper by P. polymyxa strains were conducted and only one isolate (KNUC265) was selected. Further studies into ISR mediation by the KNUC265 strain against the soft-rot pathogen Erwinia carotovora subsp. carotovora in tobacco demonstrated that the tobacco seedlings exposed to either bacterial volatiles or diffusible metabolites exhibited a reduction in disease severity. In conclusion, ISR and plant growth promotion triggered by P. polymyxa isolates were systemically investigated on pepper for the first time. The P. polymyxa KNUC265 strain, which elicited both ISR and plant growth promotion, could be potentially used in improving the yield of pepper and possibly of other crops.

Selection in the Generations after Backcrosses of the Crosses for Incorporation of Resistance to Bacterial Spot into Korean Local Cultivars of Pepper (고추 재래품종에 더뎅이병(반점세균병) 저항성을 도입하기 위한 여교잡 이후 세대에서의 선발)

  • 김병수;김점순;임양숙
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.437-444
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    • 1996
  • 경북지역 재래종에 더뎅이병 저항성을 도입하기 위하여 작성한 칼미초$\times$PI271322, 수비초$\times$PI271322 조합의 BC\ulcornerF\ulcorner와 BC\ulcornerF\ulcorner세대로부터 BC\ulcornerF\ulcorner와 BC\ulcornerF\ulcorner세대까지 선발과 자식을 실시하였다. 이 결과 각 재래종의 원예적 특성에 PI271322에 들어있는 과민반응형 저항성 유전자 Bs\ulcorner와 비과민형의 일반저항성을 함께 가지는 계통이 육성되었다.

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Copper Resistance and Race Distribution of Xanthomonas campestris pv. vesicatoria on Pepper in Korea (한국에서의 더뎅이병균의 구리 저항성과 레이스 분포)

  • 이승돈;조용섭
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.150-155
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    • 1996
  • 1993년과 1994년에 걸쳐 한국의 고추 재배 지역에서 분리한 고추 더뎅이병균은 66개의 균주중 24개가 race 1이었으며, 나머지는 42균주가 race 3이었다. Race 2균주는 발견되지 않았다. 총 66개의 균주중 8%가 200 $\mu\textrm{g}$/ml의 황산구리에 저항성을 보였으며, 100 $\mu\textrm{g}$/ml의 streptomycin sulfate에는 단지 한 균주만이 저항성을 나타냈다. Race 1균주중 21%가 구리에 저항성을 보였으나 race 3균주는 모두 구리에 감수성을 나타냈다. 모든 감수성 균주는 구리 농도 1 $\mu\textrm{g}$/ml에서 죽거나 아주 낮은 수준만이 생존하였으나 저항성 균주는 128 $\mu\textrm{g}$/ml에서도 생존하였다.

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