• 한국패류학회지
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• 제15권1호
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• pp.13-20
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• 1999

A horizontal starch gel electrophoresis for enzyme proteins extracted from three Korean species and one Chinese species of Semisulcospira was carried out in order to elucidate their genetic relationships. A total of 10 enzymes were employed in three different of buffer systems. Two loci from each enzyme of GAPDH, GOT, ICDH, IDH and PEP(VL); three loci from each of three enzymes, EST, PEP(LGG) and PGDH; and five loci from GPI were observed. Most of the loci in three pleurocerid species employed showed homozygous monomorphic banding patterns and some of them were specific as genetic markers between two different species. However, EST-2, PEP(LGG-3) and PGDH-1 loci in Korean S. libertina and PEP(LGG-3), PGM-1 and PGM-2 loci in Chinese S. libertina showed polymorphic banding patterns. Three Korean Semisulcospira species including S. libertina were more closely clustered in a dendrogram within the range of genetic identity values of 0.818-0.936, and these clusters were lineated with Chinese S. libertina at the value of 0.621.

• 한국식품과학회지
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• 제31권4호
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• pp.1101-1108
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• 1999

대두가수분해물 분무건조 펩타이드(SoyPep)가 자발성고혈압 횐쥐(spontaneously hypertensive rat, SHR)의 혈중지질수준, 생화학적지표 및 조직병리학적 변화에 미치는 영향을 조사하였다. SHR을 무작위로 대조군, 3% SoyPep 투여군, 10% SoyPep 투여군으로 구분하여 8주동안 실험사육하였다. 혈중지질 및 항산화성 비타민 수준, GOT 및 GPT활성을 측정하였고, 신장, 혈관조직, 혈장중 ACE 활성을 측정하였다. 또한, 각 주요장기의 조직병리학적 변화를 검토하였다. 식이 SoyPep은 SHR의 혈중 중성지질, 총콜레스테롤, LDL-콜레스테롤을 저하시키며, 또한, ACE저해활성 및 혈중 지질 수준의 개선작용을 나타내는 것이 확인되었다. 이들 결과로 보아, 대두 펩타이드는 혈중 지질수준의 증가를 수반하는 질병의 예방을 위한 새로운 기능성 식품소재로서의 활용가치가 매우 높을 것으로 기대된다.

• 전자공학회논문지
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• 제53권11호
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• pp.15-22
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• 2016

위성통신에서는 긴 전송 지연 시간과 지상망 대비 상대적으로 높은 데이터의 손실로 전송 속도가 저하된다. 본 논문에서는 XOR 코딩 기법과 전송 지연시간에 의한 전송성능 감소를 줄인 Hybla TCP의 장점들을 결합한 연결 분할 방식의 Hybrid TCP PEP 기법을 제안한다. 테스트베드를 구축하여 전송속도를 실험한 결과, 제안한 기법을 적용 시 채널의 에러율이 높은 환경에서 파일 전송속도가 10% 이상 향상되었다. 따라서, XOR 코딩 기법과 TCP Hybla를 결합한 Hybrid TCP를 연결 분할 방식 PEP에 적용 시 위성 통신에서 전송 속도 향상에 크게 기여할 것으로 보인다.

• 한국통신학회논문지
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• 제41권1호
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• pp.58-65
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• 2016

위성 통신은 넓은 지역에 통신 서비스를 제공하는 광역 네트워크이다. 하지만, 위성 통신은 대역폭이 제한되고 매우 긴 전파 지연 시간과 높은 BER(Bit Error Rate)로 인해 위성 통신을 고려하지 않은 TCP의 성능이 저하 된다. 본 논문에서는 위성 통신에서의 TCP 성능을 향상시키기 위해 DVB-RCS(Digital Video Broadcasting-Return Channel via Satellite) 네트워크 환경을 고려한 cross-layer 기반 PEP(Performance Enhancing Proxy) 기술을 제안한다. 제안하는 프로토콜은 TCP와 링크 계층 간 위성 자원 할당 정보를 정보 교환을 통해 최적의 TCP CWND(Congestion Window)를 설정한다. 제안한 프로토콜의 성능 평가를 위해 리눅스 기반의 PEP 테스트 베드를 구현하였다. 성능 평가 결과 다양한 BER에서 제안한 프로토콜은 위성 자원 할당 정보를 이용하여 최적의 TCP CWND 크기를 설정하기 때문에 단일 및 다중 세션 환경에서 기존 TCP 보다 제안한 프로토콜이 더 좋은 성능을 보여준다는 것을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.457-464
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• 2006

Three extracellular proteases (Vpr, peptidase T, and subtilisin) were identified from the culture supernatant of Bacillus subtilis KCTC 3014. All the proteins were partially purified as a mature form by using a DEAE-cellulose ion-exchange column chromatography. Their activities were determined by using zymography and densitometry. The relative molecular masses of Vpr and peptidase T (PepT) were determined to be 68 and 48 kDa by SDS-PAGE and zymography, respectively. However, subtilisin formed a 'binding mode' at the top of the separating gel. After denaturation by boiling at $100^{\circ}C$ for 5 min, its molecular mass was determined to be 29 kDa, whereas its activity was lost. The optimal pH of Vpr, PepT, and subtilisin were 9.0, 6.0-7.0, and 7.0-8.0, respectively. The optimal temperature of Vpr, PepT, and subtilisin was 40, 50, and $40^{\circ}C$, respectively. Inhibitor test revealed that Vpr and subtilisin were serine proteases and that PepT was a metalloprotease. Interestingly, we found that Vpr showed no enzyme activity on a 2DE zymogram gel. Three genes, vpr, pepT, and apr (encoding subtilisin protein), were cloned and their nucleotide and deduced amino acid sequences were determined.

• The Plant Pathology Journal
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• 제24권2호
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• pp.152-158
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• 2008

A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnamdo, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PePMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3'-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoV and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19 % were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoV and only <61% with PVY. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

• Journal of Preventive Medicine and Public Health
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• 제50권3호
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• pp.210-216
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• 2017

Objectives: One way to prevent deaths due to rabies is the timely utilization of post-exposure prophylaxis (PEP). Therefore, in addition to an understanding of the epidemiological distribution of animal bites, it is necessary to explore the factors leading to delays in PEP initiation. Methods: This cross-sectional study was conducted in Iran in 2011, and included 7097 cases of animal bites recorded at the Rabies Treatment Center of the Shiraz University of Medical Sciences using the census method. Logistic regression was used to identify factors associated with delays in PEP. Results: Among the patients studied, 5387 (75.9%) were males. The prevalence of animal bites in Fars province was 154.4 per 100 000 people. Dogs were the most frequent source of exposure (67.1%), and the most common bitten part of the body was the hands (45.5%). A delay in the initiation of PEP was found among 6.8% of the studied subjects. This delay was more likely in housewives (odds ratio [OR], 4.66; 95% confidence interval [CI], 2.12 to 10.23) and less likely in people with deep wounds (OR, 0.65; 95% CI, 0.43 to 0.97). Conclusions: Although all animal bite victims received complete PEP, in some cases, there were delays. Further, the type of animal involved, the depth of the bite, and the patient's occupation were the major factors associated with a delay in the initiation of PEP for rabies prevention.

• Clinical Endoscopy
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• 제56권2호
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• pp.214-220
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• 2023

Background/Aims: This study aimed to determine whether vitamin C in addition to indomethacin decreases the occurrence and severity of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis (PEP) occurrence and severity. Methods: This randomized clinical trial included patients undergoing ERCP. The participants were administered either rectal indomethacin (100 mg) plus an injection of vitamin C (500 mg) or rectal indomethacin (100 mg) alone just before ERCP. The primary outcomes were PEP occurrence and severity. The secondary amylase and lipase levels were determined after 24 hours. Results: A total of 344 patients completed the study. Based on intention-to-treat analysis, the PEP rates were 9.9% for indomethacin plus vitamin C plus indomethacin and 15.7% for indomethacin alone. Regarding the per-protocol analysis, the PEP rates were 9.7% and 15.7% in the combination and indomethacin arms, respectively. There was a remarkable difference between the two arms in PEP occurrence and severity on intention-to-treat and per-protocol analyses (p=0.034 and p=0.031, respectively). The post-ERCP lipase and amylase concentrations were lower in the combination arm than in the indomethacin alone arm (p=0.034 and p=0.029, respectively). Conclusions: Vitamin C injection in addition to rectal indomethacin reduced PEP occurrence and severity.

• BMB Reports
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• 제41권5호
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• pp.408-413
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• 2008

Pyridoxal-5'-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5'-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced PEP-1-PLPP fusion protein was stable for 36 h. The concentration of PLP was markedly decreased by the addition of exogenous PEP-1-PLPP to media pretreated with the vitamin $B_6$ precursors; pyridoxine, pyridoxal kinase and pyridoxine-5'-phosphate oxidase into cells. The results suggest that the transduction of the PEP-1-PLPP fusion protein can be one mode of PLP level regulation, and to replenish this enzyme in the various neurological disorders related to vitamin $B_6$.

• BMB Reports
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• 제44권5호
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• pp.329-334
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• 2011

Many proteins with poor transduction efficiency were reported to be delivered to cells by fusion with protein transduction domains (PTDs). In this study, we investigated the effect of levosulpiride on the transduction of PEP-1 ribosomal protein S3 (PEP-1-rpS3), and examined its influence on the stimulation of the therapeutic properties of PEP-1-rpS3. PEP-1-rpS3 transduction into HaCaT human keratinocytes and mouse skin was stimulated by levosulpiride in a manner that did not directly affect the cell viability. Following 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice, levosulpiride alone was ineffective in reducing TPA-induced edema and in inhibiting the elevated productions of inflammatory mediators and cytokines, such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1${\beta}$, and tumor necrosis factor-${\alpha}$. Anti-inflammatory activity by PEP-1-rpS3 + levosulpiride was significantly more potent than by PEP-1-rpS3 alone. These results suggest that levosulpiride may be useful for enhancing the therapeutic effect of PEP-1-rpS3 against various inflammatory diseases.