• 한국약용작물학회지
• /
• 제28권1호
• /
• pp.21-28
• /
• 2020

Background: Owing to its high efficiency in lipid and protein production, peanut (Arachis hypogaea L.) is considered one of most important crops world-wide. The kernels of peanuts are undoubtedly the most important product this plant, whereas the skin is almost completely neglected in nutraceutical terms. However, peanut skin contains potentially health-promoting phenolics and dietary fiber, and there is considerable potential for commercial exploitation. In this study, we evaluated the α-glucosidase inhibitory activity of an extract of peanut skin (PS). Methods and Results: The α-glucosidase inhibitory effects of 80% ethanol extracts of peanut (A. hypogaea L. 'Sinpalkwang') skin were evaluated and found to have a half-maximal inhibitory concentration (IC₅₀) value of 1.2 ㎍/㎖. Progress curves for enzyme reactions were recorded spectrophotometrically, and the inhibition kinetics revealed time-dependent inhibition with enzyme isomerization. Furthermore, using ultra-high performance liquid chromatography combined with quadrupole-orbitrap mass spectrometry, we identified 26 compounds in the peanut skin extract, namely, catechin, epicatechin, and 24 proanthocyanidins. Conclusions: The results suggest that peanut skin can be utilized as an effective source of α-glucosidase inhibition in functional foods and nutraceuticals.

• 한국육종학회지
• /
• 제43권2호
• /
• pp.133-138
• /
• 2011

The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.

• 한국작물학회지
• /
• 제42권3호
• /
• pp.333-337
• /
• 1997

땅콩의 육종년한을 단축하기 위한 세대촉진 방법을 정립하고자 포장후건에서 신대광땅콩 및 남대땅콩을 재배하고, 자방병이 지하로 침투한 후부터 80일까지 10일 간격으로 꼬투리와 종실을 재취하여 그 크기와 무게, 수분함량의 변화를 추적하는 동시에 종실의 발아율과 발아한 유묘의 활력을 조사한 결과, 자방병이 지하로 침투한 후 30∼40일에 꼬투리와 종실의 크기가 완성되었고, 이 때에 수확한 종실은 100％ 발아력을 갖추며 발아 후 활력있는 유묘를 생산할 수 있는 것으로 나타났다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2018년도 추계학술대회
• /
• pp.185-185
• /
• 2018

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2020년도 추계학술대회
• /
• pp.123-123
• /
• 2020

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2021년도 추계학술대회
• /
• pp.197-197
• /
• 2021

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.614-617
• /
• 1998

Anthracnose of peanut (Arachis hypogaea L.) was found in the peanut cultivating fields in Iksan, Korea in September 1997. Infected plants showed irregularly circular water soaking brown lesions. In the severe case, leaves and stems were entirely died. The causal fungus of anthracnose isolated from the diseased plants was identified as Colletotrichum gloeosporioides Penz. and its teleomorph was Glomerella cingulata (Stonem.) Spauld. & Sch. according to the criteria based on the cultural and morphological characteristics. By arificial inoculation with fungal spores on healthy peanut, anthracnose symptom was observed 15 days after inoculation.

• 한국자원식물학회지
• /
• 제24권3호
• /
• pp.272-279
• /
• 2011

It is very crucial to evaluate the genetic diversity of peanut genetic resources for identification of peanut germplasm accessions and variety improvement. Cultivated peanut generally has two subspecies, hypogaea and fastigiata. In this study, we identified peanut into three plant types, virginia (var. hypogaea), spanish (var. vulgaris), and valencia (var. fastigiata). Former one belongs to ssp. hypogaea and latter two are involved in ssp. fastigiata. Twenty SSR markers were used to assess the genetic variation of three sets, hypogaea, vulgaris, and fastigiata, respectively. Out of variety-specific SSR primers tried in this study, ten pairs of SSR primers showed polymorphisms. Each accession could be identified by a specific set of polymorphic SSR primers, and allele number was evaluated among accessions, with an average of 6.7 in var. hypogaea and 5.4 in var. vulgaris and fastigiata. For evaluation of genetic diversity, gene diversity ranged from 0.336 to 0.844 and PIC (polymorphism information contents) ranged from 0.324 to 0.827 were investigated. Dendrograms based on genetic distances were constructed, which showed the existence of three different clusters. And these three different clusters might be associated with the genes involved in three plant types. The results also suggested that there were plentiful SSR polymorphisms among peanut germplasm accessions in RDA (Rural Development Administration, Korea) Genebank and SSRs might play an important role in evaluating peanut accessions and cultivar improvement.

• Journal of Plant Biotechnology
• /
• 제2권1호
• /
• pp.21-24
• /
• 2000

An efficient method of shoot regeneration of peanut is described. In vitro shoot organogenesis from the callus of cotyledon explants of Arachis hypogaea L. was stimulated by addition of Adenine sulphate (Ads) along with 6 - benzylaminopurine (BAP) and - napthalene acetic acid (NAA). Ads (13 ${\mu}{\textrm}{m}$) had a stimulatory effect on shoot bud differentiation when combined with BAP (13 ${\mu}{\textrm}{m}$) and NAA (2 ${\mu}{\textrm}{m}$). Shoot organogenesis was markedly higher (92%) from callus induced on Ads, BAP and NAA combined media than from those formed by the individual supplementation of Ads or BAP with NAA. The shoots elongated on the media with GA$_3$ (1 ${\mu}{\textrm}{m}$). Elongated plantlets rooted with MS media containing IBA (9 ${\mu}{\textrm}{m}$).

• Journal of Plant Biotechnology
• /
• 제7권3호
• /
• pp.187-193
• /
• 2005

A protocol was developed for somatic embryogenesis with 100% induction rate from immature zygotic embryo axes of peanut (Arachis hypogaea L. cv. DRG-12) cultured on MS medium containing $18.09\;{\mu}M$ 2,4-D. The frequency of somatic embryogenesis (31.7%) as well as the number of somatic embryos induced per explant (6.6) decreased when the concentration of 2,4-D was increased to $72.4\;{\mu}M$. Morphologically abnormal somatic embryos were observed at a frequency of 43.3% on MS medium containing $72.4\;{\mu}M$ 2,4-D. Somatic embryos isolated from 30-day-old cultures of immature zygotic embryo axes exhibited precocious germination with varied responses when placed on MS basal medium with 3% sucrose. Maximum shoot induction (80.0%) was observed from somatic embryos isolated from 60-day-old cultures of immature zygotic embryo axes when placed as a clump rather than individually on MS medium supplemented with $26.63\;{\mu}M$ BA and $0.54\;{\mu}M$ NAA. Shoots developed from somatic embryos rooted with higher frequency (93.3%) on Blaydes' medium containing $5.4\;{\mu}M$ NAA.