• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.33-33
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• 2021

자웅산(264.3m)은 DMZ 접경지역인 경기도 파주시 법원읍에 위치하며, 백두대간 줄기 중 한북정맥을 따라 동쪽으로는 노고산(401m), 서쪽으로는 명학산(220m) 사이에 위치하고 있다. 2020년 4월부터 10월까지 계절별로 1회씩 총 3회 식물상 조사를 진행하였고 화상자료와 증거표본을 확보하였다. 본 조사로부터 자웅산 일대에 자생하는 관속식물은 82과 208속 267종 9아종 22변종 4품종으로 총 303분류군이 확인되었다. 양치식물은 10과 18분류군, 나자식물은 2과 6분류군, 쌍자엽식물이 62과 226분류군, 단자엽식물이 8과 53분류군으로 확인되었다. 개나리(Forsythia koreana (Rehder) Nakai), 백운산원추리(Hemerocallis hakuunensis Nakai), 키버들(Salix koriyanagi Kimura ex Goerz) 등 3과 3분류군의 특산식물이 확인되었다. 침입외래식물로는 가시도꼬마리(Xanthium italicum Moretti), 단풍잎돼지풀(Ambrosia trifida L.), 미국개기장(Panicum dichotomiflorum Michx.), 서양민들레(Taraxacum officinale F.H.Wigg.), 애기망초(Conyza parva Cronquist) 등 7과 23분류군으로 조사되어 조사식물 303분류군의 7.6%를 차지하였다. 환경부 지정 식물구계학적 특정종은 V등급이 미나리냉이(Cardamine leucantha (Tausch) O.E.Schulz), IV등급이 당단풍나무(Acer pseudosieboldianum (Pax) Kom.), 산복사나무(Prunus davidiana (Carrière) Franch.) 등 2분류군, III등급이 금낭화(Dicentra spectabilis (L.) Lem.), 꽃쥐손이(Geranium platyanthum Duthie), 분비나무(Abies nephrolepis (Trautv. ex Maxim.) Maxim.) 등 7분류군이 확인되어 III등급 이상의 특정종은 10분류군으로 파악된다.

• International Journal of Oral Biology
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• 제49권2호
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• pp.48-52
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• 2024

This study explores the histological features and Bmp4 expression patterns in the replaced tooth germ of Xenopus laevis. Tooth germ formation starts from the dental placode through epithelial-mesenchymal interactions, involving various signaling pathways such as Fgf, Shh, Bmp, and Wnt. In mice, Bmp4 expression in the dental placode inhibits Pax9 expression in the dental mesenchyme. Although absent in the presumptive dental lamina of birds and toothless mammals, Bmp4 remains conserved in reptiles and fish owing to gene duplication. However, its expression in amphibian tooth germs is poorly understood. Three-month-old X. laevis were employed in this study. Initially, samples underwent paraffin embedding and were sectioned into 5 or 12 ㎛ ribbons for H&E staining and in situ hybridization, respectively. Results revealed teeth appearing in two maxillary rows: the labial side, with prefunctional and functional teeth, and the lingual side, with replaced tooth germs behind functional teeth. Enameloid was observed between the inner dental epithelium and dental mesenchyme at the cap or early bell stages, whereas enamel and dentin formed during the late bell or mineralization stages from the replaced tooth germ. Bmp4 expression was evident in the inner dental epithelium (ameloblasts), dental papilla (odontoblasts), stellate reticulum, and Hertwig's epithelial root sheath. Overall, these findings highlight the conservation of Bmp4 expression in X. laevis tooth development.

• 한국안전학회지
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• 제39권3호
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• pp.50-59
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• 2024

In recent years, building architecture has become increasingly complex and larger in scale to accommodate many people. In densely populated facilities, the interiors are becoming more intricate and high-rise, with narrow corridors, hallways, and stairs. This poses challenges for evacuating occupants in case of emergencies such as fires, making it crucial to assess the evacuation safety in advance. In evacuation safety research, there are significant limitations to theoretical studies owing to their association with crowd behavior and human evacuation characteristics, as well as the risks associated with experiments involving human participants. Consequently, evacuation experiments conducted using simulation-based methodologies are gaining recognition worldwide. However, crowd simulations face validation difficulties because of variations in crowd movement and evacuation characteristics across different cases and scenarios, as well as the challenge of accurately reflecting human characteristics during evacuations. In this study, we investigated validation methods for evacuation simulations using the SAFEGUARD validation data set (SGVDS) provided by the University of Greenwich, UK. The SGVDS collects data on crowd evacuations through actual evacuation tests conducted on ColorLine's large RO-PAX ferry and Royal Caribbean International's cruise ships. The accuracy of the crowd simulations can be validated by comparing SGVDS and crowd simulation results. This study will contribute to the development of highly accurate crowd simulations by verifying various crowd simulations.

• 한국약용작물학회지
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• 제8권3호
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• pp.274-282
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• 2000

사람주나무 (Sapium japonicum) 종실유의 물리 화학적 특성과 구성성분을 다양한 분석법으로 구명하였다. 사람주나무의 종실유를 구성하는 지질은 중성지질이 93% 로 가장 높고 , glycolipid 가 4.9%, phospholipid가 1.3%로 나타났다. GC 분석 결과 사람주나무의 종실에는 9종의 지방산이 존재하였다. 종실유에서 지방산의 함량은 저장기간 등에 따라 약간의 차이를 보였다. 종실유 중 3종의 지질은 silica open column을 통해 분획하고, 이들의 지방산 성분을 조사하였다. 3종의 지질 중 주요 지방산은 linoleic acid와 linolenic acid로 나타났다. 채취직후의 종실유에서 지방산의 함량은 저장된 종실유의 지방산에 비해 전반적으로높았다. 이상의 결과로 보아 사람주나무의 종실유는 화장품, 세정제, 의약품 등으로의 이용이 기대된다.

• 대한두경부종양학회지
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• 제29권2호
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• pp.75-78
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• 2013

영아의 설근부에 발생하는 악성 종양은 매우 드물다. 저자들은 연하곤란과 폐쇄성 수면 무호흡증으로 의뢰된 17개월 남아의 설근부에 발생한 횡문근육종을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 외래에서 실시한 이학적 검사 상 설근부 전체가 돌출되어 있었으며 단단한 종괴가 촉지 되었다. 조직검사와 기관절개술을 시행하였으며 컴퓨터단층촬영, 양전자 방출 단층 촬영 그리고 뼈 스캔과 함께 염색체 분석을 실시하였다. 조직검사 결과 폐포성 횡문근육종이 확인되었으며 전이의 증거는 없었다. 염색체 분석상 폐포성 횡문근육종에 상응하는 PAX7-FKHR 유전자 전좌가 발견되었다. 8 회의 항암화학요법과 방사선 치료 후 촉지되는 설근부의 종괴는 없었으며 환자가 호소하는 증상도 개선되었다. 추적 관찰 시 시행된 자기공명영상 결과 확연한 종괴 크기의 감소를 확인할 수 있었다. 횡문근육종은 매우 드문 악성 종양으로 수술과 함께 항암화학요법, 방사선치료 등 여러 치료 방법이 동원되지만 전이나 재발이 있을 시 예후는 매우 불량하다. 그러므로 영아에서 연하곤란, 호흡 곤란 등의 증상이 있을 시에는 설근부를 포함한 상부호흡소화관을 적극적으로 검사하여야 하며 악성 종양의 가능성을 염두에 두어야 하겠다.

• Molecules and Cells
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• 제41권7호
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• pp.695-702
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• 2018

The inner ear is a complex sensory organ responsible for hearing and balance. Formation of the inner ear is dependent on tight regulation of spatial and temporal expression of genes that direct a series of developmental processes. Recently, epigenetic regulation has emerged as a crucial regulator of the development of various organs. However, what roles higher-order chromatin organization and its regulator molecules play in inner ear development are unclear. CCCTC-binding factor (CTCF) is a highly conserved 11-zinc finger protein that regulates the three-dimensional architecture of chromatin, and is involved in various gene regulation processes. To delineate the role of CTCF in inner ear development, the present study investigated inner ear-specific Ctcf knockout mouse embryos (Pax2-Cre; $Ctcf^{fl/fl}$). The loss of Ctcf resulted in multiple defects of inner ear development and severely compromised otic neurogenesis, which was partly due to a loss of Neurog1 expression. Furthermore, reduced Neurog1 gene expression by CTCF knockdown was found to be associated with changes in histone modification at the gene's promoter, as well as its upstream enhancer. The results of the present study demonstrate that CTCF plays an essential role in otic neurogenesis by modulating histone modification in the Neurog1 locus.

• 대한임상검사과학회지
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• 제46권3호
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• pp.91-98
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• 2014

Primary gastric lymphoma (PGL) is derived from mucosa-associated lymphoid tissue (MALT) and it differs from nodal lymphoma in histologic features and biologic behavior. Recent studies have showed that Helicobacter pylori (H.pylori ) infection is closely related to the development of low grade gastric lymphoma, and eradication of the infection induces regression of the tumor. H. pylori infection is known to be important to the development of gastric MALT lymphoma. The aim of this study was to elucidate the histopathological behavior of PGL according to the concept of MALT and to compare the predictive value of tests frequently used for diagnosis of H. pylori. The histological features of gastric lymphoma arising from MALT are the replacement of glands by uniform dense infiltration of centrocyte-like cells in the lamina propria and lymphoidepithelial lesion. H. pylori-associated histologic changes of neutrophilic infiltration, lymphoid follicle or aggregates formation and intestinal metaplasia, and H. pylori immunoreactivity were analyzed. Detection of H. pylori in chronic active gastritis and peptic ulcer suggests a possible role of H. pylori in the pathogenesis. Giemsa, Toluidine blue and Long H&E stains were used in H. pylori detection. Histopathological examination of gastric biopsy specimens revealed lymphoepithelial lesions pathognomonic of MALT lymphoma, and immunohistochemical staining for CD20 was diffusely positive. CD3 was positive in reactive T cells. PAX-5 was negative except the follicle. Bcl-2, cytokeratin, Ki-67, and c-myc were positive. The findings may indicate a predictable transition of low grade to high grade, and c-myc may be used as a valuable marker before molecular pathology diagnosis.

• 운동영양학회지
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• 제24권2호
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• pp.11-21
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• 2020

[Purpose] Doxorubicin (DOX) is a potent anti-cancer drug that appears to have severe myotoxicity due to accumulation. The skeletal muscle has a regeneration capacity through satellite cell activation when exposed to extracellular stimulus or damage. Endurance exercise (EXE) is a therapeutic strategy that improves pathological features and contributes to muscle homeostasis. Thus, this study investigated the effect of EXE training in mitigating chronic DOX-induced myotoxicity. [Methods] Male C57BL/6J mice were housed and allowed to acclimatize with free access to food and water. All the mice were randomly divided into four groups: sedentary control (CON, n=9), exercise training (EXE, n=9), doxorubicin treatment (DOX, n=9), doxorubicin treatment and exercise training (DOX+EXE, n=9) groups. The animals were intraperitoneally injected with 5 mg/kg/week of DOX treatment for 4 weeks, and EXE training was initiated for treadmill adaptation for 1 week and then performed for 4 weeks. Both sides of the soleus (SOL) muscle tissues were dissected and weighed after 24 hours of the last training sessions. [Results] DOX chemotherapy induced an abnormal myofiber's phenotype and transition of myosin heavy chain (MHC) isoforms. The paired box 7 (PAX7) and myoblast determination protein 1 (MYOD) protein levels were triggered by DOX, while no alterations were shown for the myogenin (MYOG). DOX remarkably impaired the a-actinin (ACTN) protein, but the EXE training seems to repair it. DOX-induced myotoxicity stimulated the expression of the forkhead box O3 (FOXO3a) protein, which was accurately controlled and adjusted by the EXE training. However, the FOXO3a-mediated downstream markers were not associated with DOX and EXE. [Conclusion] EXE postconditioning provides protective effects against chronic DOX-induced myotoxicity, and should be recommended to alleviate cancer chemotherapy-induced late-onset myotoxicity.

• Journal of Ginseng Research
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• 제48권2호
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• pp.163-170
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• 2024

Background: Mechanisms of synaptic plasticity in retinal ganglion cells (RGCs) are complex and the current knowledge cannot explain. Growth and regeneration of dendrites together with synaptic formation are the most important parameters for evaluating the cellular protective effects of various molecules. The effect of ginsenoside Rg1 (Rg1) on the growth of retinal ganglion cell processes has been poorly understood. Therefore, we investigated the effect of ginsenoside Rg1 on the neurite growth of RGCs. Methods: Expression of proteins and mRNA were detected by Western blot and qPCR. cAMP levels were determined by ELISA. In vivo effects of Rg1 on RGCs were evaluated by hematoxylin and eosin, and immunohistochemistry staining. Results: This study found that Rg1 promoted the growth and synaptic plasticity of RGCs neurite by activating the cAMP/PKA/CREB pathways. Meanwhile, Rg1 upregulated the expression of GAP43, Rac1 and PAX6, which are closely related to the growth of neurons. Meantime, H89, an antagonist of PKA, could block this effect of Rg1. In addition, we preliminarily explored the effect of Rg1 on enhancing the glycolysis of RGCs, which could be one of the mechanisms for its neuroprotective effects. Conclusion: Rg1 promoted neurite growth of RGCs through cAMP/PKA/CREB pathways. This study may lay a foundation for its clinical use of optic nerve diseases in the future.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8533-8539
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• 2016

Background: B-cell chronic lymphocytic leukemia B (B-CLL), the most common type of leukemia, may be caused by apoptosis deficiency in the body. Adipose tissue-derived mesenchymal stem cells (AD-MSCs) as providers of pro-apoptotic molecules such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), can be considered as an effective anti-cancer therapy candidate. Therefore, in this study we assessed the role of tumor necrosis factor-producing mesenchymal stem cells oin apoptosis of B-CLL cells resistant to fludarabine-based chemotherapy. Materials and Methods: In this study, after isolation and culture of AD-MSCs, a lentiviral LeGO-iG2-TRAIL-GFP vector containing a gene producing the ligand pro-apoptotic with plasmid PsPAX2 and PMDG2 virus were transfected into cell-lines to generate T293HEK. Then, T293HEK cell supernatant containing the virus produced after 48 and 72 hours was collected, and these viruses were transduced to reprogram AD-MSCs. Apoptosis rates were separately studied in four groups: group 1, AD-MSCs-TRAIL; group 2, AD-MSCs-GFP; group 3, AD-MSCs; and group 4, CLL. Results: Observed apoptosis rates were: group 1, $42{\pm}1.04%$; group 2, $21{\pm}0.57%$; group 3, $19{\pm}2.6%$; and group 4, % $0.01{\pm}0.01$. The highest rate of apoptosis thus occurred ingroup 1 (transduced TRAIL encoding vector). In this group, the average medium-soluble TRAIL was 72.7pg/m and flow cytometry analysis showed a pro-apoptosis rate of $63{\pm}1.6%$, which was again higher than in other groups. Conclusions: In this study we have shown that tumor necrosis factor (TNF) secreted by AD-MSCs may play an effective role in inducing B-CLL cell apoptosis.