• Animal cells and systems
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• 제4권2호
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• pp.145-150
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• 2000

Retinoic acid (RA) evokes pattern duplication in the regenerating salamander limb. Interestingly, it also enhances dedifferentiation in the regenerate by the morphological, histological and biochemical criteria. To examine whether there is any correlation between the RA-evoked pattern duplication and de novo protein synthetic profile in the regenerating salamander limb, especially during dedifferentiation, we analyzed stage-specific protein synthesis pattern in the normal and RA-treated regenerating limbs by metabolic labeling followed by two-dimensional gel electrophoresis. In the regenerating limbs without RA treatment, a few hundred kinds of proteins were found to be synthesized at the stage of wound healing and the total number of protein synthesized increased greatly as regeneration proceeded. The same trend was also observed in the RA-treated regenerating limbs. Interestingly, some protein spots were noted to be either newly synthesized or highly expressed by the RA treatment especially at the stage of dedifferentiation. The results shows that the enhancement of dedifferentiation state after the RA treatment correlates well with the protein synthesis profile, and suggest that those proteins are important for the RA-evoked pattern duplication in the regenerating limbs of salamander.

• Animal cells and systems
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• 제14권2호
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• pp.73-81
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• 2010

In this study, monoclonal antibodies against lysosomal acid phosphatase (LAP) of a salamander, Hynobius leechii, were used to determine the spatial and temporal expression of the LAP in the regenerating limbs. The Western blot and immunohistochemical analysis in the limb regeneration revealed that LAP was highly expressed at the dedifferentiation stage, especially in the wound epidermis and dedifferentiating limb tissues such as muscle and cartilage. With RA treatment, the LAP expression became upregulated in terms of both level and duration in the wound epidermis, blastemal cell and dedifferentiating limb tissues. In addition, in situ activity staining of LAP showed a similar result to that of immunohistochemistry. Thus, the activity profile of LAP activity coincides well with the expression profile of LAP during the dedifferentiation period. Furthermore, to examine the effects of lysosomal enzymes including LAP on salamander limb regeneration, lysosome extract was microinjected into limb regenerates. Interestingly, when the lysosome extract was microinjected into limb regenerates with a low dose of RA($50\;{\mu}g/g$ body wt.), skeletal pattern duplication occurred frequently in the proximodistal and transverse axes. Therefore, lysosomal enzymes might cause the regenerative environment and RA plays dual roles in the modification of positional value as well as evocation of extensive dedifferentiation for pattern duplication. In conclusion, these results support the hypothesis that dedifferentiation is a crucial event in the process of limb regeneration and RA-evoked pattern duplication, and lysosomal enzymes may play important role(s) in this process.

• 정보학연구
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• 제9권4호
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• pp.57-63
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• 2006

The intelligent elder brother groove network robot service the new broadband presence line is conversionce application service. Robot control data transmission hazard UDP packet of the remote control data which stands duplication necessary to transmit. TCP the error ratio to be high qualitative recording transmission pattern it of the transmission unit and is irregular the distance is distant to show and also the transmission lag is visible increases. The recording packet drop whose UDP degree error ratio will be high is frequent and does not arrive packet little by little increases is a possibility of knowing in the transmission unit. The technique which it proposes with traffic pattern of the transmission unit is visible the transfer characteristic of the same shape from 1% packet error ratio degree. The effective transmission technique of the robot control data which puts a base in UDP protocols was proposed from the present paper. Following research it leads and it follows the duplication transmission number of time in error rate of radio link and it was thought all that controls petty the research of the mechanism which progresses is necessary.

• Molecules and Cells
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• 제24권2호
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• pp.215-223
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• 2007

The genes encoding non-specific lipid transfer proteins (nsLTPs), members of a small multigene family, show a complex pattern of expressional regulation, suggesting that some diversification may have resulted from changes in their expression after duplication. In this study, the evolution of nsLTP genes within the Poaceae family was characterized via a survey of the pseudogenes and unigenes encoding the nsLTP in rice pseudomolecules and the NCBI unigene database. nsLTP-rich regions were detected in the distal portions of rice chromosomes 11 and 12; these may have resulted from the most recent large segmental duplication in the rice genome. Two independent tandem duplications were shown to occur within the nsLTP-rich regions of rice. The genomic distribution of the nsLTP genes in the rice genome differs from that in wheat. This may be attributed to gene migration, chromosomal rearrangement, and/or differential gene loss. The genomic distribution pattern of nsLTP genes in the Poaceae family points to the existence of some differences among cereal nsLTP genes, all of which diverged from an ancient gene. The unigenes encoding nsLTPs in each cereal species are clustered into five groups. The somewhat different distribution of nsLTP-encoding EST clones between the groups across cereal species imply that independent duplication(s) followed by subfunctionalization (and/or neofunctionalization) of the nsLTP gene family in each species occurred during speciation.

• 한국광학회:학술대회논문집
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• 한국광학회 2005년도 제16회 정기총회 및 동계학술발표회
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• pp.120-121
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• 2005

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.303-310
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• 2011

We characterized a cytoplasmic actin gene locus in greenling Hexagrammos otakii (Scorpaeniformes). Genomic clones isolated from the greenling DNA library contained two homologous cytoplasmic actin gene copies (HObact2.1 and HObact2.2) in a tail-to-head orientation. Their gene structure is characterized by six translated exons and one non-translated exon. Exon-intron organization and the nucleotide sequences of the two actin gene isoforms are very similar. However, only the HObact2.1 isoform contains microsatellite-like, dinucleotide repeats in the 5'-flanking region (named HOms2002) and intron 1 following the non-translated exon 1 (named HOms769). One microsatellite locus (HOms769) was highly polymorphic while the other (HOms2002) was not. Based on bioinformatic analysis, different transcription factor binding motifs are related to stress and immune responses in the two actin isoforms. Semiquantitative and real-time reverse transcription-PCR assays showed that both isoform transcripts were detectable ubiquitously in all the tissues examined. However, the basal expression levels of each isoform varied across tissues. Overall, the two isoforms showed a similar, but not identical, expression pattern. Our data suggest that the cytoplasmic actin genes may be the result of a recent duplication event in the greenling genome, which has not experienced significant subfunctionalization in their housekeeping roles.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2008년도 추계학술발표대회
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• pp.275-278
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• 2008

데이터마이닝은 현재 매우 각광 받고 있는 분야다. 연관규칙탐사는 트랜잭션 데이터베이스에서 일정빈도 이상의 패턴을 찾아내는 작업을 말한다. 그중 빈발서브그래프패턴 마이닝은 최근 관심이 늘어나고 있으며, 그 활용도 또한 매우 높다. 그래프마이닝은 아이템셋마이닝보다 훨씬 더 많은 계산을 필요로 한다. 중복을 최소화 하는 방법이 필요하며, 그중 가장 좋은 성능을 보이는 GASTON 알고리즘을 분석한다.

• 대한족부족관절학회지
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• 제5권2호
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• pp.120-128
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• 2001

Purpose: This study was undertaken to review the precise morphological patter'ns of polydactyly of the foot and to evaluate outcomes of surgical interventions. Materials and Methods: The records of patients who had polydactyly of foot from 1983 to 2000 were reviewed retrospectively. 29 duplicated toes(24 patients) were analyzed. All cases were evaluated and classified with Temtamy and McKusick's axial pattern, Watanabe's morphologic pattern and level of duplication. Clinical evaluation was performed according to Phelps and Grogan's. Mean age at surgery was 5.7 years(range: 10 months$\sim21$ years). All the patients were subsequently evaluated during mean 17 months(range: 13 months-$\sim5$ years). Results: Preaxial polydactyly was seen in 3 cases, central polydactyly in 3 cases, postaxial polydactyly in 22 cases and remaining 1 case was multiple my involvement. Only 2 patients have familial history and the most common associated anomaly was hand deformity(20.8%). Clinical results were excellent in 14 cases, good in 5 cases, poor in one. 17 patients(70%) were operated before 6 years olds and they had better results than those of who were operated after 6 years olds. Conclusion: The Method of surgical correction for the polydactyly should be individualized by its morphological pattern because the purpose of operation was to give comfort in wearing shoes and patient's psychological relief. This study showed that polydactyly of the foot could be corrected surgically with good results in most cases, and the better results would be achieved if the operation is performed before preschool age according to its individualized pattern of duplication.

• 한국의류학회지
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• 제17권3호
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• pp.391-406
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• 1993

The purpose of this study was to utilize of apparel CAD System by the classification of the basic pattern block in pattern making process. Gerber AM-300 CAD System was used for this study. The results form this study were as follows; 1. New shirts block were developed. 2. The sensory test was applied to evaluate the new shirts block for women by comparing it with Japanese Bunka shirts blouse pattern making method. According to a statistical analysis of the result of the 20 items on the questionnaire, the 19 items showed significant difference(${\alpha}{\leq}0.01$)between the two, with the new shirts block having higher scores. 3. A basic pattern block was selected by the design sketch. 4. P/D/S were enabled to be constructed directly from a block pattern. The drawing, deletion. duplication, and movement of all points and lines in the pattern might be made freely, and the split, pivot, and movement of the pattern, and the attachment of two patterns were possible. 5. Automatic grading of finished pattern have been developed by creation and modification of grading rules of block pattern.

• 복식
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• 제22권
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• pp.345-356
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• 1994

The Shirts Blouse was produced through the process of shirts Blouse pattern making by utilizing CAD System developed by PAD Sys-tem Techologies Co. Ltd that is suitable to educational use or industrial use. The purpose of this study was to provide the basic data for the development of efficient and various design patterns by applying this pro-cess to the actual business of apparel making. The results were as follows: 1. The applicable range of CAD system 1) The repeated work can be avoided by thansforming and developing the basic pattern stored and the storage and remodification were easy. 2) The deletion duplication movement and copy of all points and lines in the pattern can be made freely and measurement of all lengths and angles the attachment of two patterns were possible. 3) The grading Library of the basic pattern was applied as it was and the time was curtailed by adding only several grading points with the change of design. 2. The problem in the use of the CAD System 1) Through the input of basic pattern by the digitizer the minute size difference was generated as each point on the curve came to be designated. 3) In grading the line of neck girth there was inconveniences to go to the plan view in order find out deviation.