• Title/Summary/Keyword: Pathogenic Microorganisms

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PCR detection of food-borne pathogenic microorganisms in milk

  • Kim, Gyeong-Ju;Lee, Gi-Se
    • 한국생물공학회:학술대회논문집
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    • pp.204-205
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    • 2001
  • Milk is easily contaminated by pathogenic microorganisms and contains many ingredients that inhibit normal PCR. In this study, we developed a detection mothed for pathogenic microorganisms existing in milk by usting PCR. 'Sample pretreatment prior to PCR were compared to overcome the inhibition. A high PCR efficiency was achieved by SDS lysis pretreatment. without further purification of DNA for PCR.

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A study on the contamination level of pathogenic microorganisms in beef distribution stages (소고기의 유통 단계별 병원성 미생물 오염도에 관한 연구)

  • 박성도;김용환;고바라다;김철희;윤병철;김조균
    • Korean Journal of Veterinary Service
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    • v.25 no.2
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    • pp.117-126
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    • 2002
  • Contamination levels of pathogenic microorganisms in 145 cases of beef, which were distributed in Gwangju province, had been investigated in each distributed stage and also monitored by general bacterial count and E coli count index. General bacterial count of beef from the slaughterhouse was 10$^4$cfu/g less than the level of promotion(10 cfu/$\textrm{cm}^2$) and E coli count index was also under the level of 10$^2$cfu/$\textrm{cm}^2$ recommended level of the ministry of agriculture and forestry. Pathogenic microorganisms were detected from 23.2% of samples in the consumption stage, 12.5% in the slaughtering stage and 5.6% in the transporting and processing stage. Staphylococcus aureus was isolated in the largest number and its ratio was 9.0%, listeria monocytogenes 5.5% and salmonella spp 1.4%. There were no samples that bacteria had been detected dually. E coli O157:H7 and campylobacter jejuni were not isolated. In raw and chilled beef, isolation rate of pathogenic microorganisms were 13.3% and 16.5% each. Especially in raw beef, L monocytogenes was. isolated in 3 samples among 30 cases (10%) and S aureus in one sample (3.3%). According to a scale of meat store, isolation rates of pathogenic microorganisms were different. It was 28.6% in the small-scale meat store and 16.7% in the large-scale meat store each. Four cases (16.7%) of S aureus were isolated in the large-scale meat store and seven cases (20.0%) of L monocytogenes and 2 cases (5.7%) of salmonella spp were isolated in the small-scale meat store. S aureus was isolated in two places among 10 feeding facilities of the elementary school. This result shows that the sanitation of elementary school feeding facilities is so poor and more careful policy consideration is needed. Eleven strains of S aureus isolated showed ${\beta}$-hemolysis on blood agar, 1 strain ${\alpha}$-hemolysis, and 1 strain ${\gamma}$-hemolysis. Isolated strains of L monocytogenes were reconfirmed in 560 bp by PCR. Conclusively, these results show that the sanitary condition in the stages of slaughtering, transportation-processing and consumption influences the degree of pathogenic microorganisms contamination in beef severely It is necessary to apply thoroughly hazard analysis critical control point in a process of beef distribution and also to develop rapid test methods for microorganism diagnosis. This effort is very important for the supply of safe and clean meat from farm to table and helpful for the improvement of public health.

Inhibition of Spoilage and Pathogenic Bacteria by Lacticin JW3, a Bacteriocin Produced by Lactococcus lactis JW3 Isolated from Commercial Swiss Cheese Products

  • Jeong, Min-Yong;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • pp.558-561
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    • 2000
  • Strain JW3 was isolated from commercial Swiss cheese products and identified as a bacteriocin producer. Lactococcus lactis JW3 showed a broad spectrum of activity against most of the non-pathogenic and pathogenic microorganisms tested by the modified deferred method. Lacticin JW3 also showed a relatively broad spectrum of activity against non-pathogenic and pathogenic microorganisms as assessed using the spot-on-lawn method. It demonstrated a typical bactericidal mode of inhibition against Leuconostoc mesenteroides KCCM 11324.

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Seasonal Dynamics of Pathogenic Microorganisms (Cryptosporidium, Giardia and Fecal Bacteria) in an Artificial Lake Ecosystem (Sangsa Lake, Korea)

  • Kim, Sung-Hyun;Kim, Hyun-Woo;Lee, Hak-Young;Kahng, Hyung-Yeel
    • Journal of Ecology and Environment
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    • v.31 no.2
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    • pp.161-165
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    • 2008
  • This study was performed for the purpose of monitoring monthly levels of two pathogenic microorganisms, Cryptosporidium and Giardia, from November 2005 to August 2007 in Sangsa Lake. Water temperatures, pH and DO fluctuated seasonally at the study site. Annual mean values of BOD, COD and SS were $0.8\;mg\;L^{-1}$, $2.3\;mg\;L^{-1}$ and $1.9\;mg\;L^{-1}$ respectively. Although there was distinct seasonal variation in water chemistry and chlorophyll $\underline{a}$ concentration, the lake generally contains low concentrations of nutrients and chlorophyll $\underline{a}$. The relative abundance of coliform bacteria was always greater than that of fecal coliform. The fecal coliform bacteria comprised $8.5{\sim}22.1%$ of total coliform bacteria. Seasonal analysis of Cryptosporidium and Giardia levels in the study site showed that in winter (November through February), Cryptosporidium oocysts and Giardia cysts were most abundant ($1.1{\sim}1.8\;{\times}\;10\;cells\;L^{-1}$ and $3.8{\sim}5.1\;{\times}\;10\;cells\;L^{-1}$, respectively), while in summer (July through September) the abundance was lowest ($0.0{\sim}0.3\;{\times}\;10\;cells\;L^{-1}$ and $0.9{\sim}2.9\;{\times}\;10\;cells\;L^{-1}$, respectively). Molecular identification revealed two subtypes of Cyrptosporidium parvum in Sangsa Lake.

Screening of Herb Drugs Showing Antimicrobial Activity Against Some Pathogenic Microorganisms (일부 병원성 미생물에 대해 항균활성을 보이는 생약의 탐색)

  • 곽이성;양재원;이광승
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.141-145
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    • 1993
  • Thirty nine kinds of Korean herb drugs have screened for antimicrobial activity of some pathogenic microorganisms. It was revealed that some of hot water extracts from herb drugs showed antimicrobial activity in one or more strain of pathogenic microorganisms. Phellodendron amurense and Coptis chinensis inhibited growth of Staphylococcus aureus. Rubus coreanus showed antibacterial activity in Staphylococcus aureus and Pseudomonas aeruginosa. Citrus unshill inhibited growth of Escherichia coli and Cornus officinalis showed antibacterial activity in E. coli and Pseudomonas aeruginosa. Dioscorea battltas and Cinnamomum cassia showed antibacterial activity in Pseodomonas aeruginosa. And also, ScutelJa baicaJerrsis inhibited growth of Candida albicanus. Achyranthes japonica and Glycyrrhiza uralensis showed antifungal activity in Aspergillus niger. It was noteworthy that Glycrrhiza uranensis inhibited growth of Staphylococcus aureus, E. coli, Pseudomonas aeruginosa and Aspergillus niger.

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Antagonistic inhibitory effects of probiotics against pathogenic microorganisms in vitro (Probiotics의 병원성미생물에 대한 길항적 억제효과)

  • Yuk, Young Sam;Lee, Young ki;Kim, Ga-Yeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.110-116
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    • 2019
  • To investigate the antagonistic inhibitory effects in a mixed culture between probiotics and various pathogenic microorganisms, 140 probiotics were identified using a 16 rRNA sequencing phylogenetic analysis method, and various probiotics strains were isolated from Korean kimchi from January to December 2016. The antagonistic inhibition test of a mixed culture of four probiotics (Enterococcus faecalis, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus reuteri) with excellent antimicrobial activity and six pathogenic microorganisms (Candida albicans, Salmonella Enteritidis, E. coli O157:H7, Shigella flexneri, Staphylococcus aureus, and Pseudomonas aeruginosa)showed that the growth of most probiotics strains increased normally after culture, but growth was inhibited almost completely in most pathogenic microorganisms, except for S. Enteritidis. This antagonistic inhibitory effect in vitro was attributed to the low pH of the lactic acid and organic acid produced during fermentation. As a result, four probiotics strains isolated from Korean Kimchi are very likely to be developed as therapeutic agents for female yeast infections and colon and skin care. In the future, these therapeutic agents will help improve public health related to probiotics.

Lantibiotics, Class I Bacteriocins from the Genus Bacillus

  • Lee, Hyung-Jae;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.229-235
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    • 2011
  • Antimicrobial peptides exhibit high levels of antimicrobial activity against a broad range of spoilage and pathogenic microorganisms. Compared with bacteriocins produced by lactic acid bacteria, antimicrobial peptides from the genus Bacillus have been relatively less recognized despite their broad antimicrobial spectra. These peptides can be classified into two different groups based on whether they are ribosomally (bacteriocins) or nonribosomally (polymyxins and iturins) synthesized. Because of their broad spectra and high activity, antimicrobial peptides from Bacillus spp. may have great potential for applications in the food, agricultural, and pharmaceutical industries to prevent or control spoilage and pathogenic microorganisms. In this review, we introduce ribosomally synthesized antimicrobial peptides, the lantibiotic bacteriocins produced by members of Bacillus. In addition, the biosynthesis, genetic organization, mode of action, and regulation of subtilin, a well-investigated lantibiotic from Bacillus subtilis, are discussed.

Antimicrobial Effect of Furaneol Against Human Pathogenic Bacteria and Fungi

  • Sung Woo-Sang;Jung Hyun-Jun;Lee In-Seon;Kim Hyun-Soo;Lee Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.349-354
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    • 2006
  • Furaneol, a key aroma compound found in strawberry, pineapple, and processed foodstuffs, has been known to possess various biological activities on animal models. In this study, the antimicrobial effects of furaneol against human pathogenic microorganisms were investigated. The results indicated that furaneol displayed a broad spectrum of antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi without hemolytic activity on human erythrocyte cells. To confirm the antifungal activity of furaneol, we examined the accumulation of intracellular trehalose as a stress response marker on toxic agents and its effect on dimorphic transition of Candida albicans. The results demonstrated that furaneol induced significant accumulation of intracellular trehalose and exerted its antifungal effect by disrupting serum-induced mycelial forms. These results suggest that furaneol could be a therapeutic agent having a broad spectrum of antimicrobial activity on human pathogenic microorganisms.

Microbial change of pork carcass during processing in small size slaughterhouse (소규모 돼지도축공정에서 도체오염 미생물의 변화)

  • 홍종해;이경환;이성모
    • Korean Journal of Veterinary Service
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    • v.25 no.1
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    • pp.31-37
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    • 2002
  • Major hazards existed in slaughterhouse are pathogenic microorganisms originated from intestinal microflora of slaughtered animals. This study was intended for the identification of microbial contamination sources during pork slaughtering in small plants. Total aerobic bacteria, Coliform group, Salmonella spp, Listeria monocytogenes, and Campylobacter jejuni/coli were isolated from the surface sample of pork carcasses. Contamination level among different sampling points of ham, belly and neck did not showed statistical differences. Therefore, the mixed sampling from belly and neck of carcass could be effective for microbiological monitoring. Isolation rates of pathogenic microorganisms showed Salmonella spp 20.9%, Listeria monocytogenes 10.5%, and Campylobacter jejuni/coli 8.1% from 296 sampling points. High prevalence rate of Salmonella spp indicated that the contamination of intestinal microflora occurred due to unsanitary processing control, which required HACCP system in small plants. It was recommended that the prerequisite program should be a key factor for a successful HACCP system implementation especially in small size slaughterhouse.

Antibacterial Activities of Phenolic Components from Camellia sinensis L. on Pathogenic Microorganisms

  • Shin, Jung-Sook;Chung, Ha-Sook
    • Preventive Nutrition and Food Science
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    • v.12 no.3
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    • pp.135-140
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    • 2007
  • Antibacterial activities of the major phenolic components from Camellia sinensis L. were investigated against several pathogenic microorganisms including Gram-positive strains like Staphylococcus aureus ATCC 29213 and Streptococcus pyogens 308A; and Gram-negative strains like Escherichia coli ATCC 25922, Escherichia coli 078, Pseudomonas aeruginosa 9027, and Enterobacter cloacae 1321E. The MIC values demonstrate that both (-)-epicatechin and (-)-epigallocatechin were more considerably toxic against Staphylococcus aureus ATCC 29213 than the other two catechins like (-)-epicatechingallate and (-)-epigallocatechin-3-gallate. (-)-Epicatechingallate and (-)-epigallocatechin-3-gallate were most inhibitory against Escherichia coli ATCC 25922. As a result, (-)-epicatechin showed predominant antibacterial activities among tea varieties. The contents of major polyphenolic components such as four catechins, theaflavin, and quercetin were different according to fermentation processes. The total contents of four catechins were ranged from 13.81 to 1.33%, with (-)-epigallocatechin-3-gallate being dominant among tea varieties; theaflavin was found the characteristic pigment in fully-fermented black tea.