• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.427-428
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• 1989

• 한국자동차공학회논문집
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• 제3권5호
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• pp.17-23
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• 1995

A numerical analysis on engine coolant is made by the use of FVM based general purpose 3 dimensional Navier-Stokes solver, TURB-3D. Numerical solutions are verified by comparison with the experimental data of Lotus model. The results show a good qualitative as well as quantitative comparison. Coolant flow rate control is attempted through adjusting the cross section area of passage base on the results of an original coolant passage. It is concluded from the results that the flow rate control is possible as attempted, and thus can be used in the real engine design.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.287-288
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• 1989

• 항공우주시스템공학회지
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• 제9권4호
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• pp.67-72
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• 2015

The analysis has been performed on the blockage effect at the propellant flow passage in a liquid rocket engine. This simulates an example of emergency situation where flow passage is partially blocked. The analysis method has been validated by predicting the pump head and flow rate within 1% precision against the measured data of turbopump-gas generator coupled test. When the oxidizer passage is reduced it is predicted that the mixture ratio decreases, the oxidizer pump head increases and the gas generator pressure increases. When the fuel passage is reduced it is predicted that the mixture ratio increases, fuel flow rate decreases and the fuel pump head increases.

• 대한건축학회논문집:계획계
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• 제34권6호
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• pp.135-142
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• 2018

A public pedestrian passage induces traffic into the apartment complex, and therefore, it requires the application of Crime Prevention Through Environmental Design(CPTED). This paper aims to analyze the conditions and to suggest the improvements of public pedestrian passages in apartment complexes, applying a 3-D approach from CPTED aspect. The first aspect is "Designation": 1)The linkage between the public pedestrian passages is weak, and their designated purpose becomes unclear. 2)When the designated purpose is not considered for each complex, the closure rate is high. 3)The closure rate of the "Through type" is high. It suggests that the pedestrian network should be built and vitalized at the local urban level. The second aspect is "Definition." The public pedestrian passage lacks a social, cultural, and legal definition and only has a passive physical definition. It suggests that a legal definition and specialized guildline be prepared. The third aspect is "Design": 1)Insufficient territoriality exists between the public pedestrian passage and the inside of the complex. Territoriality should be reinforced between the public pedestrian passage and the rest of the internal territory. 2)The landscaping adjacent to the public pedestrian passage lacks deliberation. Since this is where safety and privacy collide, detailed design and maintenance of the landscaping is required. 3)It lacks systematical crime prevention facilities and should compensate accordingly.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.194-200
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• 2009

The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithelial cells from 1-2, 3-6 and 7-10 passages were used for the nuclear transfer. In the oocytes with the cumulus donor cells, fusion and cleavage rates of oocytes and cell numbers per blastocyst among the three different passage groups did not show any differences, but the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher than those from 7-10 passage group. The rates of fusion, cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the embryos with the sizes of <20 and 20 ${\mu}m$ cumulus donor cells compared to the >20 ${\mu}m$ cumulus donor cell. In the oocytes with the fetal fibroblast donor cells, the rate of blastocyst formation from the 3-6 passage group was higher than from 1-2 and 7-10 passage groups. The embryos with the size of 20 $\mu{m}$ fetal fibroblast donor cell showed higher rate of blastocyst formation compared to those with <20 and >20 ${\mu}m$ donor cells. In the oocytes with the oviductal epithelial cells, the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher compared to those from 7-10 passage group. The embryos with the sizes of <20 and 20 ${\mu}m$ oviductal epithelial donor cells had a higher rate of blastocyst formation compared to those with >20 ${\mu}m$ donor cell. Fusion and cleavage rates of oocytes, and cell numbers per blastocyst among the three different donor cell types from the 3-6 passage did not show any differences. However, the rate of blastocyst formation of somatic cell nuclear transfer (SCNT) embryos with the fetal fibroblast donor cell was higher than that of blastocyst formation of SCNT embryos with the cumulus and oviductal epithelial donor cells.

• Asian-Australasian Journal of Animal Sciences
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• 제4권2호
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• pp.137-141
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• 1991

Rumen liquor characteristics and disappearance rate of dry matter were studied in Kedah-Kelantan cattle and swamp buffaloes fed grass of rice straw-based diet. Cobalt-EDTA and chromium mordented fibres prepared from the faecal material were used to determine the liquid and solid particles movement in both animal species fed with rice straw. Swamp buffaloes showed a more intense rumen fermentation activity than Kedah-Kelantan cattle when both species were fed straw-based diet. The buffaloes also demonstrated faster rates of grass and straw degradation in situ. The fluid outflow rate from the rumen of buffalo ($1.06{\pm}0.19l/h$) was observed to be slower than that of cattle ($1.55{\pm}0.01l/h$). No significant differences between cattle and buffaloes were observed in rumen fluid volume and passage rate of small particles from the rumen.

• 대한환경공학회지
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• 제35권12호
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• pp.967-972
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• 2013

질소산화물 저감에 큰 효과가 있는 MILD 연소는 고온의 배기가스가 연소로내에 유입되는 양에 따라 질소산화물 저감 특성이 많은 영향을 받는다. 본 연구에서는 동심원관 형태의 MILD 연소로에서 바깥 원통의 배기가스 통로에서부터 안쪽 원통의 연소통로 사이에 연결관을 설치하고 배기가스를 유입하기 위해 coanda 노즐을 사용하였다. 이러한 coanda 노즐의 기하학적 형상 변화에 따라 고압공기 유량, 배기가스 유입량 특성을 수치해석을 통해 살펴봄으로써 최적의 coanda 노즐 형상을 도출하는 것을 본 연구의 목적으로 하였다. 본 연구의 전산 해석의 결과는 conada 노즐의 노즐 통로 간격이 0.5 mm, 노즐 각도 $4^{\circ}$, 노즐 확관 길이 146 mm일 때 최적의 유입량비가 되었고 노즐 통로 수직 길이는 유입량비에 무관하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.44-47
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• 2000

Cell growth and DNA synthesis were studied from a cultured early- and late- pas- sage mouse aorta smooth muscle cell (MASMC) because the proliferation of vascular smooth muscle cell (VSMC) is a key factor in development of atherosclerosis. In this study, the cells were cultured in fetal bovine serum (FBS) and stimulated by growth factors such as thrombin and platelet-derived growth factor-BB (PDGF-BB). Compared to the number of early-passage MASMC (passage 3 to 9) the number of late-passage MASMC (passage 30 to 40) in a normal serum state was increased 2 fold at Day 1, 3 and 6 in culture, respectively. Incorporation of $[^3H]$ thymidine into DNA induced by serum, PDGF and thrombin in late-passage MASMC was greater than those in early-passage MASMC. We also examined whether intracellular zinc levels would be an aging factor or not. The intracellular zinc level in early- and late-passage MASMC was monitored by using the zinc probe dye N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide. It is interested that late-passage MASMC increased the intracellular fluorescence level of zinc, more than the early passage MASMC did. The alterations of intracellular zinc level occur concurrently with changes in MASMC proliferation rate during aging. This data suggest that the age-associated changes in zinc concentrations may provide a new in vitro model for the study of smooth muscle cell differentiation.

• Reproductive and Developmental Biology
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• 제28권1호
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• pp.13-20
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• 2004

This study was conducted to investigate the developmental ability of caprine embryos after somatic cell interspecies nuclear transfer. Donor cells were obtained from an ear-skin biopsy of a caprine, digested with 0.25% trypsin-EDTA in PBS, and primary fibroblast cultures were established in TCM-199 with 10% FBS. After maturation, expanded cumulus cells were removed by vigorous pipetting in the presence of 0.3% hyaluronidase. The matured oocytes were dipped in D-PBS plus 10% FBS＋7.5 $\mu\textrm{g}$/ml cytochalasin B and 0.05 M sucrose. The reconstructed oocytes were electrically fused with donor cells in 0.3 M mannitol fusion medium. After the electofusion, embryos were activated by electric stimulation. Interspecies nuclear transfer embryos with bovine cytoplasts were cultured in TCM-199 medium supplemented with 10% FBS including bovine oviduct epithelial cells for 7∼9 day. On the other hand, the NT embryos with porcine cytoplasts were cultured in NCSU-23 medium supplemented with 10% FBS for 6∼8 day at $39^{\circ}C, 5% CO_2$ in air. In caprine-bovine NT embryos, the cleavage(2-cell) rate was 36.8% in confluence and 43.8% in serum starvation. The developmental rate of morula- and blastocyst-stage embryos was 0.0% in confluence and 18.8% in serum starvation. In caprine-porcine NT embryos, the cleavage(2-cell) rate was 76.7% in confluence and 66.7% in serum starvation. The developmental rate of morula and blastocyst stage embryos was 3.3% in confluence and 3.0% in serum starvation, and no significant difference was observed in synchronization treatment between donor cells. In caprine-bovine NT embryos, the cleavage(2-cell) rate of cultured donor cells was 30.8% and 17.6% in 5∼9 and 10∼14 passage(P＜0.05). The developmental rate of morula and blastocyst stage embryos were significantly higher(P＜0.05) in 5∼9 passage(23.1%) than in 10∼14 passage(0.0%) of cultured donor cells. In caprine-porcine NT embryos, the cleavage rate was significantly higher(P＜0.05) in 5∼9 passage(86.7%) than in 10∼14 passage(50.0%) of cultured donor cells. The developmental rate of morula and blastocyst stage embryos were 3.3 and 0.0% in 5∼9 and 10∼14와 passage of cultured donor cells. In caprine-bovine NT embryos, the developmental rate of morula and blastocyst stage embryos were 22.6% in interspecies nuclear transfer, 33.9% in in vitro fertilization and 28.1% in parthenotes, which was no significant differed. The developmental rate of morula and blastocyst stage embryos with caprine-porcine NT embryos were lower(P＜0.05) in interspecies nuclear transfer(5.1%) than in vitro fertiltzation(26.9%) and parthenotes(37.4%).