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Influence of NaCl and pH on Hydrolysis of Chicken Myofibrillar Proteins by Leukocyte Lysosomal Proteinases (Leucocyte lysosomal proteinase에 의한 닭의 근섬유(筋纖維) 단백질(蛋白質) 분해(分解)에 미치는 NaCl과 pH의 영향(影響))

  • Shinlee, Seung-Yee;Rhee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.22 no.5
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    • pp.569-574
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    • 1990
  • The influence of NaCl and pH on degradation of chicken breast muscle myofibrillar proteins by porcine leukocyte lysosomal proteinases was investigated. The degradation reactions were carried out at $38^{\circ}C$ for 24hours under different conditions. The degradation of myofibrillar proteins by leukocyte lysosomal enzymes at various pH values was limited to partial hydrolysis. Reactions at higher pH values resulted in lower molecular weight degradation products while reactions at lower pH resulted in higher molecular weight degradation products. When NaCl was added into the reaction mixture, enzyme activities of degradation were increased at all pH values studied, as evidenced by NPN-analysis and SDS-PAGE. More severe degradation was observed with higher salt concentration. The concentration of 0.5M NaCl in the reaction mixture gave more degradation of myosin heavy chain by enzyme than that of 0.1M NaCl.

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Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus

  • Song, Ha-Yeon;Choi, Hyo-Jin;Jeong, Hansaem;Choi, Dahye;Kim, Dae-Hyuk;Kim, Jung-Mi
    • Mycobiology
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    • v.44 no.4
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    • pp.283-290
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    • 2016
  • A double-stranded RNA (dsRNA) mycovirus was detected in malformed fruiting bodies of Pleurotus ostreatus strain ASI2792, one of bottle cultivated commercial strains of the edible oyster mushroom. The partial RNA-dependent RNA polymerase (RdRp) gene of the P. ostreatus ASI2792 mycovirus (PoV-ASI2792) was cloned, and a cDNA sequences alignment revealed that the sequence was identical to the RdRp gene of a known PoSV found in the P. ostreatus strain. To investigate the symptoms of PoV-ASI2792 infection by comparing the isogenic virus-free P. ostreatus strains with a virus-infected strain, isogenic virus-cured P. ostreatus strains were obtained by the mycelial fragmentation method for virus curing. The absence of virus was verified with gel electrophoresis after dsRNA-specific virus purification and Northern blot analysis using a partial RdRp cDNA of PoV-ASI2792. The growth rate and mycelial dry weight of virus-infected P. ostreatus strain with PoV-ASI2792 mycovirus were compared to those of three virus-free isogenic strains on 10 different media. The virus-cured strains showed distinctly higher mycelial growth rates and dry weights on all kinds of experimental culture media, with at least a 2.2-fold higher mycelial growth rate on mushroom complete media (MCM) and Hamada media, and a 2.7-fold higher mycelial dry weight on MCM and yeast-malt-glucose agar media than those of the virus-infected strain. These results suggest that the infection of PoV mycovirus has a deleterious effect on the vegetative growth of P. ostreatus.

Association between Axial Length and Anthropometric Value in Korean Children (한국 어린이의 안축장과 신체 계측치와의 관련성)

  • Kim, Hyojin;Lyu, Jungmook
    • Journal of Korean Ophthalmic Optics Society
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    • v.19 no.3
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    • pp.397-402
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    • 2014
  • Purpose: To investigate the relationship between axial length (AL) and anthropometric parameters in Korean children. Methods: This study included 40 urban school children aged 11-12 years (mean age, $11.95{\pm}0.22$ years; 45.0% girls) residing in Seoul, South Korea. AL (using partial coherence laser interferometry), corneal radius, refractive error, height (m), and weight (kg) were measured. Body mass index (BMI $[kg/m^2]=weight/[height]^2$) and degree of obesity (DO[%]=[actual weight standard weight]/standard weight) were calculated. Furthermore, the number of hours spent reading, watching television, and using a computer every day was determined using a detailed questionnaire. Results: The students had a mean spherical equivalent refraction of $1.06{\pm}0.84$ D. Weight (r=0.427, p=0.006), BMI (r=0.508, p=0.001), and DO (r=0.371, p=0.018) showed a significant positive correlation with AL. Furthermore, longer AL was significantly associated with heavier weight (p=0.041), and higher BMI (p=0.015), and higher DO quartiles (p=0.042). After adjust for age, sex, and near-work activities, multivariate linear regression models showed that weight, BMI, and DO were still significantly associated with AL. Among the near-work activities, daily reading time was significantly associated with AL. Conclusions: AL was positively related to weight as well as daily reading time in Korean urban school children.

Effect of Soil Reinforcement on Shear Strength by Pennisetum alopecuroides and Miscanthus sinensis Roots on Loamy Sand at River Banks (하천제방 양질사토에 대한 수크령과 억새 뿌리의 토양전단강도 보강효과)

  • Dang, Ji-Hee;Cho, Yong-Hyeon;Lee, Chun-Seok
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.20 no.2
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    • pp.79-91
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    • 2017
  • This study was conducted to find out the physical properties and soil shearing strength reinforcement effect of herbaceous plants for the slope revegetation works. Two native plants(Pennisetum alopecuroides and Miscanthus sinensis) were used for this experiment, because they have excellent seed germination rates without preconditioning, and grow naturally around rivers. To identify the physical properties, the partial dry weight of plants were investigated. To identify the soil shearing strength reinforcement effect, the respective soil shearing strengths of the control soils, Pennisetum alopecuroides, Miscanthus sinensis samples were measured. Also, we did a correlation analysis to examine the relation of shearing strength to plant features. The results are summarized as follows: 1. The average dry weight of Pennisetum alopecuroides samples consists of 52.36% above ground and 47.64% at root. And in dry weight, 78.24% of it's root distributes within 10 cm in soil depth. Meanwhile the average dry weight of Miscanthus sinensis samples consists of 52.91% above ground and 47.09% at root. And in dry weight, 82.95% of it's root distributes within 10 cm in soil depth. 2. The results of correlation analysis showed that for both Pennisetum alopecuroides and Miscanthus sinensis, it could not be said that there was any correlation between shearing strength and plant characteristics, and statistically they were not meaningful. 3. In the shearing strength test with control soils, Pennisetum alopecuroides, Miscanthus sinensis as subjects, the differences in shearing strength measurement results were modest, and the order was shown as control soils < Pennisetum alopecuroides < Miscanthus sinensis, so the soil shearing strength reinforcement effect by the Pennisetum alopecuroides and the Miscanthus sinensis on loamy sand at river banks surface was confirmed.

RELATIONSHIP BETWEEN TEMPERAMENTAL CHARACTERISTICS AND PHYSICAL GROWTH OF CHILDREN (아동의 기질특성과 신체발육 사이의 관계)

  • Choi, Seong-Goo;Hong, Sung-Do;Kim, S. Peter
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.8 no.1
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    • pp.43-49
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    • 1997
  • This study was designed to explore whether the temperament of a child influences the physical growth. The Korean version of the Parent Questionnaire for Children developed by Thomas, Chess and Korn was applied to 395 Korean children whose ages ranged from 3 years to 7 years. Simultaneously the height and the weight were measured for each child and converted into percentile scale according to the Growth Curve and the Weight Percentile Table for the Height of Korean children. Statistical analysis was performed among 9 temperamental categories, height and weight percentiles for the age and weight percentiles for the height using the first-order partial correlation analysis, controlling for the familial mean income per month. Results showed that the more temperamentally difficult a child is, the lower weight he has when compared with the children of the same age or the same height. Although there were some differences, the tendency of the above findings was maintained both in male and female children. These results show that the temperament may influence the physical growth as well as the psychological development.

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Genetic Analysis by Diallel Crosses in F1 Generation of Silkworm, Bombyx mori (이면교잡에 의한 가잠 F1세대의 몇 가지 양적 형질에 관한 유전 분석)

  • 정원복;장권열
    • Journal of Sericultural and Entomological Science
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    • v.28 no.1
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    • pp.24-29
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    • 1986
  • A set of 21 crosses of F1 generation by diallel crosses from seven parent silkworm, Bombyx mori L. were used as materials to estimate genetic parameters such as gene action and combining ability. Experiments were conducted in 1985, and three characters, which are weight of cocoon, weight of cocoon layer and ration of cocoon layer, were observed. The data were analyzed for each character by Jinks', Hayman's and Griffing's methods. The result obtained are summarized as follows : 1. In Vr-Wr graphical analyses, weight of cocoon was found to be inherited in over dominance, and weight of cocoon layer and ration of cocoon layer were observed as partial dominance. 2. Estimate of additive component of variance(D) was greater than those of dominance component of variance(H) for weight of cocoon layer and ratio of cocoon layer, and positive values of F were observed for all characters. 3. Mean squares of general combining ability were higher than those of specific combining ability. 4. Variety Jam 107 was expressed as the highest GCA effects for three characters, and high SCA effects for all characters were observed in combinations of 113 $\times$Jung 14 and Jam 107 $\times$Jung 14.

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A Retrospective Analysis of the Effectiveness of Taking Gambi-tang in Overweight and Obese Adults (과체중 및 비만 성인 대상 감비탕 복용 효과에 대한 후향적 분석)

  • Park, Han-song;Yoo, Hee-jung;Ha, Ji-su;Park, Hyun-seo;Seo, Ho-seok;Kim, Jin-won
    • The Journal of Internal Korean Medicine
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    • v.43 no.3
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    • pp.413-422
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    • 2022
  • Objectives: To investigate the weight loss effect of Gambi-tang in obese and overweight adults. Methods: From September 1, 2016 to December 31, 2021, a retrospective chart analysis was conducted on adult men and women who were prescribed Gambi-tang to address obesity and overweight in the National Medical Center (NMC). Partial analysis by age, obesity (Body Mass Index, BMI), duration of dose, and cumulative dose was also conducted. Results: The weight and BMI of the study subjects showed a significant decrease of more than 5% on average before and after treatment. In an analysis of age, significant differences by age could not be confirmed. In the analysis by obesity level, the effect of reducing weight, body fat, and abdominal fat rates in the obese and highly obese groups was greater than in the normal and overweight groups. There was no significant difference by group in the analysis by dosage period. In the analysis by cumulative dose, the greater the cumulative dose up to 1,800 g, the greater the decrease in weight and body fat. The side effect that patients appealed to the most was misjudgment, but not to a serious extent. Conclusions: Gambi-tang has been shown to be effective in weight and body fat loss for both obese and overweight adult men and women.

Relationship among Ginsenosides of Panax ginseng Root under the Variation of Mineral Nutrients (무기영양변화에서 인삼근Ginsenoside의 상호관계)

  • Lee, Mee-Kyoung;Min, Jin-Sook;Park, Hoon
    • Journal of Ginseng Research
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    • v.10 no.1
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    • pp.101-107
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    • 1986
  • Relationships among ginsenosides, panaxadiol(PD), panaxatriol(PT), and total saponin(TS) in Panax ginseng root (2nd Year) grown with culture solotion different in nitrogen, phosphorus and potassium level were analyzed by simple correlation, multiple regression and standard partial regression coefficient. The closeness between ginsenosides by simple correlation was closely related with the similarity of molecular structure. The content of PT was much attributed to Re and Rg1. The contribution order of ginsenosides for PD was Rb1>Rb2$\geq$Rd>Rc. There was significant positive correlation between PT and PD but PD increased more rapidly than PT. Thus total saponin depended much on PD and PT/PD decreased with the increase of total saponin content. All ginsenosides, especially Re showed decreasing tendency with the increase of root weight.

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Sialoglycoproteins of Mammalian Erythrocyte Membranes: A Comparative Study

  • Sharma, Savita;Gokhale, Sadashiv M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1666-1673
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    • 2011
  • The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

Characteristics of Sweet Pumpkin Yanggaeng with Stevia Leaf Powder as Partial Replacer of Sucrose (설탕량 감소를 위한 스테비아 잎 분말 적용 단호박 양갱)

  • Choi, Eun-Hee;Chung, Chang-Ho
    • Culinary science and hospitality research
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    • v.24 no.3
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    • pp.83-92
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    • 2018
  • Sweet pumpkin yanggaeng were produced with stevia leaf powder as a partial sugar replacer(0 to 20% sucrose weight) and antioxidant. Characteristics of the yanggaeng were compared in moisture, total soluble solids, pH, color, texture, antioxidant properties, and preference scores. Moisture and total soluble solids were proportionally increased with increasing addition of stevia leaf powder(p<0.001). pH of control (without stevia) and SLP20(with 20% replacement of sucrose) were 7.05 and 6.82, respectively. L-value, a-value, and b-value were decreased with increasing amount of stevia leaf powder (p<0.001). Antioxidant activities (total polyphenols and DPPH radical scavenging activity) were gradually increased as more stevia leaf powder included in the yanggaeng(p<0.001). SLP20 being significantly different from others (p<0.001) had the highest values of hardness and chewiness, but there was no significant difference between cohesiveness and adhesiveness among samples. SLP4 had the highest preference in appearance and texture, otherwise SLP8 did the highest scores in flavor, sweetness and overall preference. SLP8, in which 8% sucrose was replaced with stevia leaf powder, was considered to be the most desirable for production of sweet pumpkin yanggaeng.