• 한국패류학회지
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• 제28권4호
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• pp.377-384
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• 2012

The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcriptional analysis in mollusks. This is the first report on the transcriptomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7959-7965
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• 2015

Background: Identification of predictive markers for the efficacy of platinum-based chemotherapy is necessary to improve the quality of the life of cancer patients. Materials and Methods: We detected proteins recognized by autoantibodies in pretreated sera from patients with lung adenocarcinoma (AC) evaluated as showing progressive disease (PD) or a partial response (PR) after cisplatin-based chemotherapy by proteomic analysis. Then, the levels of the candidate autoantibodies in the pretreated serum were validated by dot-blot analysis for 22 AC patients who received platinum-based chemotherapy, and the expression of identified proteins was immunohistochemically analyzed in 40 AC biopsy specimens. Results: An autoantibody against galectin-3 (Gal-3) was detected in pretreated sera from an AC patient with PD. Serum IgG levels of anti-Gal-3 autoantibody were significantly higher in patients evaluated with PD than in those with PR and stable disease (SD) (p = 0.0084). Furthermore, pretreated biopsy specimens taken from patients evaluated as showing PD following platinumbased chemotherapy showed a tendency to have a higher positive rate of Gal-3 than those with PR and SD (p = 0.0601). Conclusions: These results suggest that serum IgG levels of anti-Gal-3 autoantibody may be useful to predict the efficacy of platinum-based chemotherapy for patients with lung AC.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7867-7869
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• 2015

Objective: To assess the safety and effectiveness of thalidomide (produced by CHANGZHOU PHARMACEUTICAL FACTORY CO.LTD) combined with chemotherapy in treating patients with advanced colorectal cancer. Method: A consecutive cohort of pretreated patients with advanced colorectal cancer were treated with thalidomide combined with chemotherapy. And chemotherapy for patients with advanced colorectal cancer were administered according to the condition of patients. Thalidomide was orally administered at a dosage of 50mg/day to 150mg/day before sleeping for at least 14 days. After at least 14 days of treatment, safety and side effects were evaluated. Results: There were 12 female and 3 male patients with advanced cancer recruited into this study, including 9 patients with colon, 6 patients with rectal cancer. The median age of patients was 57(41-82) years. Partial response was observed in 2 patients (2/15), and stable disease in 3 patients(3/15). Incidences of Grade 1 to 2 myelosuppression was observed in 1/15 patients, and Grade 1 to 2 elevation of hepatic enzyme was recorded in 1/15 patients. Adverse effects on the gastrointestinal tract were documented in 1/15 patients, and were Grade 1. No Grade 3-4 toxicities were diagnosed. No treatment related death was found. Conclusions: Thalidomide combined with chemotherapy was safe and mildly effective in treating patients with advanced colorectal cancer. However, further study should be conducted to clarify the effectiveness of this combination.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6129-6132
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• 2012

Objectives: To evaluate the feasibility and efficacy of simultaneous accelerated radiation therapy (SMART) and concurrent weekly paclitaxel in the treatment of locally advanced nasopharyngeal carcinoma. Methods: Forty-one patients with pathologically confirmed nasopharyngeal carcinoma were treated by SMART with concurrent weekly paclitaxel. Daily fraction doses of 2.5 Gy and 2.0 Gy were prescribed to the gross tumor volume (GTV) and clinical target volume (CTV) to a total dose of 70 Gy and 56 Gy, respectively. Paclitaxel of $45mg/m^2$ was administered concurrently with radiation therapy every week. Adjuvant chemotherapy was given four weeks after the completion of the radiotherapy (RT) if the tumor demonstrated only a partial response (PR). Results: All patients completed the radiotherapy (RT) course. Adjuvant chemotherapy was administered to 12 patients due to PR. The CR (complete remission) rate was 82.9% three months after RT. Thirty-nine (95.1%) patients completed the concurrent weekly chemotherapy with paclitaxel, and two patients skipped their sixth course. Seven patients had a 15% dosage reduction at the fifth and sixth course due to grade 3 mucositis. The median follow-up was 30 (range, 14-42) months. The three-year overall survival (OS), metastases-free survival (MFS), and local control rates were 77.0%, 64.4%, and 97.6%, respectively. No correlation between survival rate and T or N stage was observed. Grade 3 acute mucositis and xerostomia were present in 17.1% and 7.1%, respectively. Conclusion: SMART with concurrent weekly paclitaxel is a potentially effective and toxicity tolerable approach in the treatment of locally advanced NPC.

• 한국잡초학회지
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• 제10권3호
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• pp.179-185
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• 1990

제초제(除草劑) thiobencarb 처리(處理)에 의한 벼 내성(耐性)의 유전양식(遺傳樣式)을 조사키 위하여 thiobencarb에 반응(反應)을 달리하는 5품종(品種)과 이들을 이용하여 이면교잡(二面交雜)으로 얻는 $F_1$ 잡종(雜種)을 대상으로 thiobencarb 2 kg 및 4 kg a.i. ha을 처리(處理)하여 이면교잡(二面交雜) 분석법(分析法)으로 조사한 결과(結果)를 요약(要約)하면 다음과 같다. Thiobencarb 농도(濃度)에 관계없이 초장(草長)과 건물중(乾物重)의 일반조합능력(一般組合能力)과 특정조합능력(特定組合能力)에서 공(共)히 유의성(有意性)이 인정(認定)되었으며 일반조합능력(一般組合能力)이 특정조합능력(特定組合能力)보다 높게 나타났다. 내성(耐性)에 관여(關與)하는 유전자(遺傳子)의 작용(作用)은 상가적(相加的) 효과(效果)가 지배적(支配的)이였으며 내성(耐性)이 높은 방향(方向)으로의 부분우성(部分優性)도 인정(認定)되었다. 협의(俠義) 및 광의(廣義)의 도전력(道傳力)은 비교적(比較的) 높게 나타났는데 특(特)히, 4kg a:/ha 처리(處理)에서 협의(俠義)의 유전력(遺傳力)이 초장(草長)에서 0.726 건물중(乾物重)에서 0.743으로 높은 편이었다. 제초제(除草劑) thiobencarb에 내성(耐性)을 나타낸 벼 IR9660-50-3-1 계통(系統)의 일반조합능력(一般組合能力) 효과(效果)는 내성(耐性)이 높은 방향(方向)으로 높고 내성(耐性)에 관여(關與)하는 우성유전자(優性遺傳子)를 많이 가진 것으로 추정(推定)되었다.

• 한국강구조학회 논문집
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• 제22권4호
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• pp.325-334
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• 2010

본 연구에서는 반강접 접합부 배치에 따른 구조물 거동특성을 파악하기 위하여 KBC2005 건축구조설계기준으로 비가새 5층 철골 구조물을 설계하여 모든 접합부를 완전 강접합부과 반강접 접합부로 이상화한 경우 그리고 반강접 접합부를 수직배치 및 수평배치한 경우에 대하여 푸쉬오버 구조해석을 실시하였다. 철골 보 및 기둥의 모멘트-곡률관계는 화이버모델을 이용하여 확인하였으며 반강접 접합부의 모멘트-회전각 관계는 3-매개변수 파워모델을 이용하여 나타내었다. KBC2005 등가정적횡하중을 받는 2차원 구조물에 대한 푸쉬오버 구조해석을 실시하여 지붕충변위, 밑면전단력, 접합부 요구연성도, 소성힌지 발생순서 그리고 초과강도계수, 연성계수, 반응수정계수와 같은 설계계수 등을 확인하였다. 반강접 접합부를 부분적으로 사용하면 모든 접합부에 반강접 접합부를 사용한 골조 보다 큰 강도 및 강성도를 확보 할 수 있었고 모든 접합부에 강접 접합부를 사용한 골조 보다 큰 연성능력을 확보할 수 있었다. 초과강도계수는 기준과 비슷하였지만 반응수정계수는 기준 보다 훨씬 큰 값을 보였다. TSD 접합부는 예제 구조물에서 경제성과 안전성을 확보할 수 있음을 확인할 수 있었다.

• Tuberculosis and Respiratory Diseases
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• 제56권2호
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• pp.216-220
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• 2004

폐전이에서 공동성 폐병변이 관찰되는 경우가 흔하지 않으며, 다른 양성 폐병변과 감별이 필요하다. 저자들은 다발성 공동성 폐병변을 주소로 내원한 환자에서 굴곡성 기관지내시경 검사를 통한 조직검사상 전이성 폐암을 진단하였고 원발성 병변인 담관암을 진단하여 전신적인 항암약물을 시행한 경험이 있어 이에 보고하는 바이다.

• 대한치과기공학회지
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• 제33권4호
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• pp.369-378
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• 2011

Purpose: This study was carried out for the purpose of obtaining basic data necessary for developing the future oral health promotion program of the elderly by surveying the actual condition of managing dental prosthesis in the elderly. Methods: It collected materials targeting 346 elderly brackets in over 65 years old who dwell in Seoul and Gyeonggi-do Province for about 10 months from September 2010 to June 2011, analyzed 312 copies except 34 copies, which are inappropriate for data processing due to being insufficient in response among these things, and obtained the following results. Results: In the actual condition of missing tooth, a case of missing tooth was indicated to be 78.8%. In case of missing tooth, the number of missing teeth was indicated to be 75.6% for under 5 pieces and 19.1% for over 21 pieces. In the actual condition of managing prosthesis, a case with dental prosthesis treatment was indicated to be 77.6%. A kind of prosthesis was indicated to be the highest in fixed partial dentures with 36.5%. In a method of rinsing dentures, 47.7% of the elderly responded as saying of washing it out with water. The appearance of removing dentures at night was indicated to be the highest in doing so with 63.1%. Satisfaction with prosthesis of being mounted now was indicated to be moderate with 50.4%. As for the appearance of having experience of education on how to manage prosthesis, it was indicated to be high with 50.4% in having none. In a kind of prosthesis depending on averagely monthly income, the ratio of total dentures was high when averagely monthly income is low. On the other hand, a case with high income was indicated to have high ratio in implants. The satisfaction with prosthesis was low when averagely monthly income is low. Also, in the experience of education on how to manage prosthesis, the ratio of responses as saying of having no experience of education on how to manage prosthesis was indicated to be high when averagely monthly income is low. Conclusion: Examining the above results, a developmental plan is considered to be necessary such as developing continuous and systematic oral health promotion program for managing elderly people's managing dental prothesis.

• 한국항해항만학회지
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• 제28권5호
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• pp.421-428
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• 2004

복잡한 수심을 가진 연안해역에서 파랑의 천수효과, 굴절, 회절, 부분반사, 해저마찰, 쇄파의 영향까지를 고러한 파랑모델의 도입은 대부분의 해안공학 설계나 방재 문제에 매우 중요한 요소이다. 파랑이 심해역에서 수심이 말은 친해역으로 이동함에 따라. 파랑의 기본적 특성이 변하며, 파에너지는 천해역과 섬, 해안 보호 구조물, 불규칙한 연안 경계와 다른 지리적 특징에 의하여 파봉선을 따라 재분산된다. 또한, 쇄파가 발생하는 쇄파대에서나, 해안선 및 구조물의 경계에서 반사된 까가 그 입사파와 상호 작용을 하는 영역을 통과하면서 격한 변화를 일으킨다. 완경사방정식 파랑모델의 현장 적용은 지금까지 여러 모델이 다루지 못한 파창변환과정을 이해하는데 도움을 줄 것이다. 본 연구에서는 영일만 신항만의 건설이 이루어질 영일만 내의 넓은 수역에 대해 파랑의 변환과정을 보다 합리적으로 해석하기 위해 확장완경사방정식 파람모델을 구성하고, 신항건설 전,후의 해면변동과 신항만 개발에 따른 포항 구항해역 및 포항 신항해역 등 인접해역에서의 정온도 변화를 비교 분석하는 것을 골자로 하고 있다. 이러한 시도가 광역항만권 개발에서 쉽게 누락시킬 수 있는 기존항만에 대한 파생적 영향을 반드시 분석하도록 하는 계기가 될 것으로 본다.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2214-2223
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• 2016

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.