• BMB Reports
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• v.43 no.6
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• pp.432-437
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• 2010

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes ${\alpha}B$-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with ${\alpha}B$-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, ${\alpha}B$-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or ${\alpha}B$-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both ${\alpha}B$-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and ${\alpha}B$-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.245-253
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• 2014

A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus using submerged culture fermentation. The enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, with a yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDS-PAGE were 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. It hydrolyzed fibrinogen by cleaving the ${\alpha}$ and ${\beta}$ chains of fibrinogen followed by the ${\gamma}$ chains, and also activated plasminogen into plasmin. The enzyme was optimally active at $45^{\circ}C$ and pH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitors of TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partial amino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR, GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree of homology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. The purified enzyme can also be applied as a natural agent for oral fibrinolytic therapy or prevention of thrombosis.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.79-90
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• 2020

Objective: In the present study, an liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was performed to investigate potential biomarkers of milk production in high- and low-milk-yield dairy cows and to establish correlations among rumen fluid metabolites. Methods: Sixteen lactating dairy cows with similar parity and days in milk were divided into high-yield (HY) and low-yield (LY) groups based on milk yield. On day 21, rumen fluid metabolites were quantified applying LC/MS. Results: The principal component analysis and orthogonal correction partial least squares discriminant analysis showed significantly separated clusters of the ruminal metabolite profiles of HY and LY groups. Compared with HY group, a total of 24 ruminal metabolites were significantly greater in LY group, such as 3-hydroxyanthranilic acid, carboxylic acids, carboxylic acid derivatives (L-isoleucine, L-valine, L-tyrosine, etc.), diazines (uracil, thymine, cytosine), and palmitic acid, while the concentrations of 30 metabolites were dramatically decreased in LY group compared to HY group, included gentisic acid, caprylic acid, and myristic acid. The metabolite enrichment analysis indicated that protein digestion and absorption, ABC transporters and unsaturated fatty acid biosynthesis were significantly different between the two groups. Correlation analysis between the ruminal microbiome and metabolites revealed that certain typical metabolites were exceedingly associated with definite ruminal bacteria; Firmicutes, Actinobacteria, and Synergistetes phyla were highly correlated with most metabolites. Conclusion: These findings revealed that the ruminal metabolite profiles were significantly different between HY and LY groups, and these results may provide novel insights to evaluate biomarkers for a better feed digestion and may reveal the potential mechanism underlying the difference in milk yield in dairy cows.

• Advances in concrete construction
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• v.15 no.6
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• pp.419-430
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• 2023

In this study, the effect of two types of aggregates (fly ash aggregate and shale aggregate) on the density, strength, and durability of preplaced lightweight aggregate concrete (PLWAC) was studied. The results showed that the 7 - 28 days strength of concrete prepared with fly ash aggregates (high water absorption rate) significantly increased, which could attribute to the long-term water release of fly ash aggregates by the refined pore structure. In contrast, the strength increase of concrete prepared with shale aggregates (low water absorption rate) is not apparent. Although PLWAC prepared with fly ash aggregates has a lower density and higher strength (56.8 MPa @ 1600 kg/m³), the chloride diffusion coefficient is relatively high, which could attribute to the diffusion paths established by connected porous aggregates and the negative over-curing effect. Compared to the control group, the partial replacement of fly ash aggregates (30%) with asphalt emulsion (20% solid content) coated aggregates can reduce the chloride diffusion coefficient of concrete by 53.6% while increasing the peak load obtained in a three-point bending test by 107.3%, fracture energy by 30.3% and characteristic length by 103.5%. The improvement in concrete performance could be attributed to the reduction in the water absorption rate of aggregates and increased energy absorption by polymer during crack propagation.

• Geomechanics and Engineering
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• v.35 no.5
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• pp.525-538
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• 2023

The development of a city is closely linked to the construction and operation of its subway system. However, constructing a new subway tunnel under an existing station is an extremely complex task, and the deformation characteristics and mechanical behavior of the new subway tunnel during the excavation process can greatly impact the normal operation of the existing station. Although the previous studies about the case of underpass engineering have been carried out, there is limited research on the condition of a newly-built subway tunnel that closely undercrossing an existing station with zero distance between them. Therefore, this study analyzes the deformation law and mechanical behavior characteristics of the preliminary lining of the underpass tunnel during the excavation process based on the real engineering case of Chengdu Metro Line 8. This study also makes an in-depth comparison of the influence of different excavation methods on this issue. Finally, the accuracy of numerical simulation is verified by comparing it with on-site result. The results indicate that the maximum bending moment mainly occurs at the floor slab of the preliminary lining, while that of the ceiling is small. The stress state at the ceiling position is less affected by the construction process of the pilot tunnel. Compared to the all-in-one excavation method, although the process of partial excavation method is more complicated, the deformation of preliminary lining caused by it is basically less than the upper limit value of the standard, while that of the all-in-one excavation method is beyond standard requirements.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.18 no.4
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• pp.998-1019
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• 2024

Mobile cloud computing is a very attractive service paradigm that outsources users' data computing and storage from mobile devices to cloud data centers. To protect data privacy, users often encrypt their data to ensure data sharing securely before data outsourcing. However, the bilinear and power operations involved in the encryption and decryption computation make it impossible for mobile devices with weak computational power and network transmission capability to correctly obtain decryption results. To this end, this paper proposes an outsourcing decryption algorithm of verifiable transformed ciphertext. First, the algorithm uses the key blinding technique to divide the user's private key into two parts, i.e., the authorization key and the decryption secret key. Then, the cloud data center performs the outsourcing decryption operation of the encrypted data to achieve partial decryption of the encrypted data after obtaining the authorization key and the user's outsourced decryption request. The verifiable random function is used to prevent the semi-trusted cloud data center from not performing the outsourcing decryption operation as required so that the verifiability of the outsourcing decryption is satisfied. Finally, the algorithm uses the authorization period to control the final decryption of the authorized user. Theoretical and experimental analyses show that the proposed algorithm reduces the computational overhead of ciphertext decryption while ensuring the verifiability of outsourcing decryption.

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2214-2223
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• 2016

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.237-242
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• 1999

As one of the key parameters to determine $CO_2$ flux between air - sea interface, it is quite important to know p$CO_2$, which has involved much uncertainty, mainly due to the complex variations of sea surface p$CO_2$ and the paucity of samples, made in ocean. In order to improve the interrelationship between partial pressure (p$CO_2$) and different physical and biochemical parameters in global sea surface water, a new empirical relation is established to correlate and parameterize p$CO_2$ in the mixed layer using the data from recent WOCE cruises. Meanwhile, by new empirical relation, abundant historical hydrographic and nutrients ship data, Levitus data set and NOAA/AVHRR(SST), p$CO_2$ have been accumulated and applied. Then effort has to be made fur promotion of this study to correlate and parameterize p$CO_2$ in the mixed Layer with different physical and biochemical parameters. and further attribute this huge historical data sets and NOAA/AVHRR(SST) data to estimate p$CO_2$. In this paper we analyzed more interrelationship between the model and ship/satellite data set. Finally, the inter-annual variations of p$CO_2$ in sea are presented and discussed.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.17 no.11
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• pp.1056-1063
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• 2007

Recently, there has been a growing consumer interest in the amount of noise produced by household electrical appliances. The designer of the product must identify the source of the noise, in order to reduce the noise. In the case of a household electric appliance such as the washing machine, there is consumer's complaint about the noise that is generated during the dehydrating condition. Because of several noise sources combined each other. It is difficult to identify the noise sources that contribute to the noise output. Multi-Dimensional Spectral Analysis (MDSA) is a method that can remove the correlation between different noise sources, and it expresses the key contributing factor as a unique output. This study utilized MDSA to analyze the contribution of each input in the noise output during the dehydrating condition.

• Proceedings of the KSR Conference
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• 2001.10a
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• pp.299-303
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• 2001

In this paper, we introduce the Partial Half-Through Type Plate Girder Bridge proposed by Hyundai Development Co. and KRTC Consortium in Turn-Key Based design last year, and this type of bridge may be adapted to the railway bridge under similar conditions. This bridge across the North Han River was to be concerned its find view and the protection of water source. For its location is inside the dam, it required the long span and the clearance for the flood also. Its new composite section was able to diminish the deflection and vibration. Its shop-fabrication could control its quality easily, and might to be launched. This structural type have satisfied its functions, construction situations, and overall safeties, so this is the field-oriented type. However the composition and connections of the proposed members are different to the recent forms, and difficult to be applied to present specifications. Therefore it requires a certification on the application for more safety by not only analytical verifications but also experimental studies. Nevertheless, this type of bridge may have some more applications from now on.