• Proceedings of the Korea Society of Poultry Science Conference
• /
• 2006.11a
• /
• pp.82-83
• /
• 2006

생식 세포는 한 세대의 유전 정보를 다음세대에 전달할 수 있는 유일한 세포로서 다양한 특성을 가지고 있다. 기능 유전체 연구를 통해서 새로운 유전자의 기능을 규명함으로써 그 유전자의 생물학적 의미와 상호작용을 설명할 수 있다. 본 실험의 목적은 생식세포에서 특이적으로 발현하는 유전자를 발굴하여 그 유전자가 생식세포의 발달과 분화에 중요한 역할을 수행하는 것을 증명하는 것이다. 이에 본 실험에서는 real-time quantitative RT-PCR 기법을 이용하여 정소에서 특이적으로 발현하는 5개(AGE1, AGE2, AGE3, AGE4, AGE5)를 선발하였고, in situ hybridization 실험을 통하여 정소 조직 내에서의 발현 양상을 확인하였다. AGE1, AGE2는 round spermatid에서 특이적으로 발현하고, AGE3, AGE4, AGE5는 spermatocyte에서 특이적으로 발현하는 것을 확인하였다. 본 실험을 통해 발굴한 유전자들은 닭의 생식선발달에 중요한 기능을 할 것으로 예상되며, 앞으로 닭의 유전육종분야에 큰 도움을 줄 수 있을 것이다.

• Journal of Environmental Science International
• /
• v.22 no.12
• /
• pp.1643-1650
• /
• 2013

This study investigated water quality of effluents and stream from the sewage treatment plants located at Gumi Complex 4, Gumi, and Wonpyeong in Gumi. Downstream region was found to increase the concentration of nutrients for sewage treatment plant effluent. Both phosphorus and nitrogen were accounted most as soluble form. In particular, the high ratio of dissolved effluent of sewage treatment plants were investigated. In the streams, Phosphorus concentration was high during rainy season and nitrogen concentration was high in the dry season. Sewage treatment plant effluent was relatively less microbial activity and nutrient concentrations were higher in the winter. TN/TP ratio was the highest in the upstream region and the lowest in the sewage treatment plant effluent. The effect of the nutrient matter from a discharge of a sewage treatment plant on rivers varied depending on the size of the river and the treatment plant. However, the influence of the concentration was greater than that of flowrate. Sewage treatment plant effluent loads phosphorus, nitrogen accounted for 8% and 6% respectively at the point N3 of the Nakdong river.

• Journal of Korean Neurosurgical Society
• /
• v.54 no.3
• /
• pp.232-235
• /
• 2013

Objective : Major complaints of carpal tunnel syndrome (CTS) are sensory components. However, motor deficit also impedes functional status of hand. Contrary to evaluation of sensory function, the objective, quantitative evaluation of median nerve motor function is not easy. The motor function of median was evaluated quantitatively using load cell and its correlation with findings of electrodiagnostic study (EDS) was evaluated. Methods : Objective motor function of median nerve was evaluated by load cell and personal computer-based measurement system. All of the measurement was done in patients diagnosed as having idiopathic CTS by clinical features and EDS findings. The strength of thumb abduction and index finger flexion was measured in each hand three times, and the average value was used to calculate thumb index ratio (TIR). The correlation of TIR with clinical, EDS, and ultrasonographic findings were evaluated. Results : The TIR was evaluated in 67 patients (119 hands). There were 14 males and 53 females, mean age were 57.6 years (range 28 to 81). The higher preoperative nerve conductive studies grade of the patients, the lower TIR was observed [p<0.001, analysis of variance (ANOVA)]. TIR of cases with thenar atrophy were significantly lower than those without (p<0.001, t-test). TIR were significantly lower in patients with severe median nerve swelling in ultrasonography (p=0.042, ANOVA). Conclusion : Measurements of median nerve motor function using load cell is a valuable evaluation tool in CTS. It might be helpful in detecting subclinical motor dysfunction before muscle atrophy develops.

• Journal of Korean Neurosurgical Society
• /
• v.54 no.6
• /
• pp.496-500
• /
• 2013

Objective : To evaluate the successful fusion rate in postmenopausal women with single-level anterior cervical discectomy and successful fusion (ACDF) and identify the significant factors related to bone successful fusion in pre- and postmenopausal women. Methods : From July 2004 to December 2010, 108 consecutive patients who underwent single-level ACDF were prospectively selected as candidates. Among these, the charts and radiological data of 39 women were reviewed retrospectively. These 39 women were divided into two groups : a premenopausal group (n=11) and a postmenopausal group (n=28). To evaluate the significant factors affecting the successful fusion rate, the following were analyzed : the presence of successful fusion, successful fusion type, age, operated level, bone mineral density, graft materials, stand-alone cage or plating with autologous iliac bone, subsidence, adjacent segment degeneration, smoking, diabetes mellitus, and renal disease. Results : The successful fusion rates of the pre- and postmenopausal groups were 90.9% and 89.2%, respectively. There was no significant difference in the successful fusion rate or successful fusion type between the two groups. In the postmenopausal group, three patients (10.8%) had successful fusion failure. In the postmenopausal group, age and subsidence significantly affected the successful fusion rate (p=0.016 and 0.011, respectively), and the incidence of subsidence in patients with a cage was higher than that in patients with a plate (p=0.030). Conclusion : Menopausal status did not significantly affect bone successful fusion in patients with single-level ACDF. However, in older women with single-level ACDF, the combination of use of a cage and subsidence may unfavorably affect successful fusion.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.10 no.2
• /
• pp.131-136
• /
• 2005

Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.36 no.4
• /
• pp.368-374
• /
• 2008

The basic Trainer Aircraft of The Korean Air force KT-1/KO-1, which was designed and developed by Korean Technology has been a primary candidate as a replacement for the aging basic trainer or armed version for surface support operation for the nations such as Mexico, Philippine, Turkey, Venezuela and etc. In this study, competitors for the same class aircraft and the present operational status of the basic trainer of the world were identified and the fade out periods were also predicted. The result of this market analysis showed the required number of aircraft by the year 2025 for 33 countries as 4500. The exportable number of KT-1 aircraft was predicted considering the political/diplomatical environments, economic environment and also by the ability of producing same class aircraft of the country.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.4 no.2
• /
• pp.36-44
• /
• 2001

The main purpose of this study was to classify forest vegetation effectively using Landsat Thematic Mapper data(June, 1994) in mountainous region. The research area was the Mt. Wolak Experimental Forest of Chungbuk National University, near Chungju and Jecheon city, Chungcheongbuk-do. To classify forest vegetation effectively, Normalized Difference Vegetation Index(NDVI) was used to reduce topographic effects. This NDVI was modified and transformed to the value of 0 to 255, and then the modified values were combined with other Landsat Thematic Mapper bands. To classify forest and land cover types, unsupervised classification method was used. The results of this study are summarized as follows. 1. Combinations of band "3, 5, NDVI" in Landsat Thematic Mapper data showed a good separation with high accuracy. The expected classification accuracy was 95.1% in Landsat Thematic Mapper data. 2. The Land Cover types were classified into six groups : coniferous forest, deciduous forest, mixed forest, paddy and grass, non-forest, and other undetectable areas. As these classified results were compared with the reconnaissance survey and aerial black and white infrared photographs, the overall classification accuracy was 76.5% in Landsat Thematic Mapper data. 3. The portion of non-forest in Mt. Wolak area was 1.9%. The percentages of coniferous, deciduous and mixed forests were 30.9%, 35.7% and 26.4%, respectively. 4. As these classified results were compared with other reference data, the percentages of coniferous, deciduous and mixed forests increased, but the portion of non-forest was exceedingly diminished. These differences are thought to be from the different research method and the different season of received Landsat Thematic Mapper data.

• Journal of Applied Biological Chemistry
• /
• v.61 no.2
• /
• pp.135-139
• /
• 2018

The inhibition of aldose reductase (AR) has been shown to prevent the progression of the many complications associated with diabetic hyperglycemia. Several compounds purified from various plant sources have exhibited potent inhibition against AR. In this study, the inhibitory effects of the methanol extracts of the flowers of Synurus excelsus and Weigela subsessilis on AR were determined in vitro. Scopolin and scopoletin are coumarins isolated from the flowers of S. excelsus and W. subsessilis; and quercetin is a known AR inhibitor present in many flowers. To determine and quantify their presence in both plants, HPLC-UV analysis of all three compounds was performed. S. excelsus and W. subsessilis showed potent inhibition against AR having $IC_{50}$ values of 0.17 and $0.14{\mu}g/mL$, respectively. The concentration of scopolin in S. excelsus and W. subsessilis were 34.71 and 174.14 mg/g extract, respectively. Scopoletin was detected in S. excelsus at 3.41 mg/g extract, whereas quercetin was not detected in both plants. This study shows that S. excelsus and W. subsessilis exhibited promising AR inhibitory effects and are both sources of coumarins.

• Journal of Biosystems Engineering
• /
• v.34 no.1
• /
• pp.63-68
• /
• 2009

This study was carried out to determine necessary conditions for optimal ventilation of small windowless piglet house (5.2 (W) ${\times}$ 12.3 (L) ${\times}$ 2.3 (H) m) with negative tunnel ventilating system using CFD (Computational Fluid Dynamics) simulation. The weaning piglet house for this experiment was consisted of 4 rooms (520 (W) ${\times}$ 300 (L) cm), 3 fences (70 (H) cm), 1 air inlet (350 (W) ${\times}$ 2 (H) cm) and 1 exhaust fan (50 (D) cm), and simulated using CFD code, FLUENT. The simulation results for the original weaning piglet house showed ununiform ventilation for each room. Therefore, to uniformly ventilate all rooms, the heights of the air inlet and first fence were modified to 3 cm and 100 cm, respectively. The simulation result f3r the modified weaning piglet house showed uniform ventilation for all rooms and the optimum air inlet velocity of 1.4 m/s.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.8 no.2
• /
• pp.99-103
• /
• 1988

With a purpose of finding out the effects of magnesium and boron enriched organic-compound fertilizer application on qualities of grasses on the hilly pasture, a field experiment was arranged with five different treatments as a randomized block design and lasted from September, 1984 to the end of growing season in 1986. The results obtained are summarized as follows: 1. Mean chemical components except NFE of grasses at the plots with organic-compound fertilizer application were appeared to contain slightly high comparing to single dressing. 2. Production of DCP with organic-compound fertilizer (1,170.2 kg/ha) was significanty increased by 20% comparing to single dressing (975.7 kg/ha) in the conventional fertilization level (p<0.05), but it was not significantly different between them in the low dressing level. 3. Production of energies (TDN, StE and NEL) with organic-compound fertilizer of low and conventional dressing level were appeared to increase by 10 and 13 to 15% than those of single dressing of the same levels, respectively. But it was not significant difference. 4. Like single dressing nutrient productions with organic-compound fertilizer were appeared significantly high due to increasing of dressing gradually (P<0.05-0.01) 5. Mineral contents of P and Mg at the plots with organic-compound fertilizer were a little higher, on the other hand, K, C/P and K/Ca + Mg equivalent ratio were slightly lower than those of single dressing, but the content of Ca tended to similar between them.