• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.120-120
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• 2017

최근 국내 소비자들의 안전한 음식과 환경문제에 대한 관심이 높아지면서 재배작물과 경작지 토양의 물리 화학적 특성 등에 맞추어 적정 시비 및 재배관리를 적용하는 정밀농업이 시도되고 있다. 정밀농업은 농약 및 비료 등의 양을 적절하게 사용하여 생산 비용을 절감할 수 있으며, 과다사용을 막아 환경오염을 줄일 수 있다. 현재 토양의 수분, EC, pH등과 같은 항목은 신속하고 간단하게 분석이 가능하지만 토양의 필수 원소인 N, P, K 성분을 분석하는 제품은 상용화 되지 않았거나 가격이 비싸 일반 농가에서는 사용이 어렵다. 특히, 토양의 인을 현장에서 분석하기 위한 연구가 많이 수행되고 있으나 실제로 현장에서 사용되는 경우 또한 없는 상황이다. 일반적으로 토양에 포함되어 있는 인을 측정하기 위해서 많은 실험실에서는 이화학적 분석법을 사용하고 있다. 하지만 이 분석법은 오랜 시간과 전문적인 인력이 필요하며 주변 환경변화에 민감하여 실시간으로 분석하기에는 어렵다고 한다. 따라서 본 연구에서는 현장에서 실시간으로 토양의 인을 분석하기 위해 반자동 분석시스템을 제작하고 이를 검증하고자 하였다. 이전 연구에서 현장 토양을 이용한 신속측정법의 가능성($R^2>0.7$)을 확인하여 이 분석법을 바탕으로 반자동 분석시스템을 제작하였고 표준결과 값의 약 70% 이상의 수준을 목표로 하였다. 진주 지역의 현장 토양 20개 샘플과 반자동 침출 장치, 여과 장치, 발색 장치 그리고 통합 반자동 시스템을 이용하여 분석한 후 표준 분석 결과와 비교하였을 때 $R^2$ 값이 각각 0.985, 0.817, 0.893, 0.837로 약 70% 이상의 수준을 달성하였다. 추후, 반자동 토양 인 신속측정시스템을 완전 자동화 시스템으로 개발하고 주행 장치를 부착시켜 현장에서 실시간으로 원하는 위치의 인을 측정 할 수 있을 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.89-93
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• 2004

Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• Korean Journal of Head & Neck Oncology
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• v.20 no.1
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• pp.3-6
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• 2004

Background and Objectives: Nodal metastasis is one of the prognostic factors in salivary gland cancer. The purpose of this study is to identify risk factors of nodal metastasis considered as a predictor of poor prognosis in patients with salivary gland cancer. Material and Methods: The authors retrospectively studied 82 patients with salivary gland cancer who underwent surgery from 1992 to 2002. We analyzed age, sex, tumor size, histologic type and 5-year survival rate to compare patients with and without nodal metastasis. Results: Mean age was 55.4 years, with a male-female ratio of 1 : 1.28. The overall 5-year survival rate was 85.5%. Among the 82 patients, 14 patients had nodal metastasis. There was no nodal metastasis in low grade malignancy. In patients without nodal metastasis, mean age was 52.5 years and the overall 5-year survival rate was 94.2%. In patients with nodal metastasis, mean age was 69.4 years and the overall 5-year survival rate was 42.9%. In patients with less advanced cancer (T1-T2 stage), the nodal metastasis was 7.5% and with advanced cancer (T3-T4 stage), 33.3%. Conclusion: Nodal metastasis significantly decreases survival in patients with salivary gland malignancy. High grade malignancy, large tumor size and old age are important risk factors of nodal metastasis. Nodal metastasis is more common in submandibular gland cancer compared with parotid gland cancer.

• KSBB Journal
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• v.26 no.3
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• pp.223-228
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• 2011

The effects of pH, glycerol concentration and salt on cell growth and ethanol production using Enterobacter aerogenes KCTC 2190 were evaluated in the anaerobic culture condition. In condition of initial pH 5, cell growth and ethanol production were highest. An initial concentration of 10 g/L of pure glycerol gave the highest cell growth and ethanol production. However, in case of over 15 g/L of pure glycerol, they decreased. The cell growth and ethanol production decreased with the increase of salt concentration. When 10 g/L of crude glycerol was used as the carbon source, the cell growth and ethanol production were $1.32\;OD_{600}$ and 3.95 g/L, respectively, which were about 94.4% and 88.5% compared to those of pure glycerol. These result indicates that the crude glycerol produced in the biodiesel manufacturing process maybe useful as a potential carbon source for ethanol production form Enterobacter aerogenes KCTC 2190.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.6
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• pp.508-516
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• 2017

The M&S (Model and Simulation) has been increasingly used in the wide area of applications and the official certification or accreditation of the M&S are becoming a mandatory requirement to prevent the risks and mishaps caused by the usage of inadequate M&Ss or by misuses of the M&S. This paper threats the VV&A (Verification, Validation, and Accreditation) for the M&S used in the development of the aerospace systems, where most M&S used in the development are derived from the physical laws and the final validation data are typically obtained through a series of flight tests after the prototype production. Considering these unique features, the paper proposes the M&S life-cycle model and development paradigm suitable for the aerospace-system development and accesses the proposed ones by comparative investigation on the relevant regulations and related literatures.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.165-172
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• 2010

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1.5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of ${\beta}$-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

• Journal of environmental and Sanitary engineering
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• v.3 no.2 s.5
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• pp.91-98
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• 1988

This study was carried out to investigate the Pollution of heavy metals in Soil, collected from the 50 sampling sites in 1986. The results were as follows; 1. In the average contents of Cd, the highest value was 0.33mg/kg in the Industrial area, the lowest value was 0.06 mg/kg in the Greenbelt and 0.10-0.22mg/kg in the other areas. 2. In the average contents of Cu, the highest value was 33.46mg/kg in the Industrial area, the lawest value was 3.79mg/kg in the Greenbelt and 4.69-12.21mg/kg in the other areas. 3. In the average contents of As, the highest value was 1.80mg kg in the Residential area, the lowest value was 0.43mg/kg in the Greenbelt and 0.64-1.65mg/kg in the other areas. 4. In the average contents of Zn, the highest value was 29.84mg/kg in the out wall of Seoul, the lowest value was 17.32mg/kg in the Greenbelt and 19.62-26.71mg/kg in the other areas. 5. In the average contents of Pb, the highest value was 17.09mg/kg in the Industrial area, the lowest value was 5.21mg/kg in the Reclamated waste and 5.67 -14.10mg/kg in the other areas. 6. Distribution of Heavy metals was Zn>Cu>Pb>As>Cd and the relations of Zn-Cd, Pb-Cd, Pb-Cu, Pb-Zn were shown the correlation significantly (P< 0.01).

• Journal of Trauma and Injury
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• v.28 no.3
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• pp.211-214
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• 2015

The primary and secondary survey was designed to identify all of a patient's injuries and prioritize their management. However 15 to 22.3% of patient with missed injuries had clinically significant missed injuries. To reduce missed injury, special attention should be focused on patients with severe anatomical injury or obtunded. Victims of blunt trauma commonly had multiple system involvement. Some reports indicate that inexperience, breakdown of estalished protocol, clinical error, and restriction of imaging studies may be responsible for presence of missed injury. The best way of reducing clinical significant of missed injuries was repeated clinical assessment. Here we report a case of severe blunt hepatic injury patient and pericardial injury that was missed in primary and secondary survey. After damage control surgery of hepatic injury, she remained hemodynamically unstable. Further investigation found cardiac tamponade during intensive care. This was managed by pericardial window operation through previous abdominal incision and abdominal wound closure was performed.

• Journal of Acupuncture Research
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• v.28 no.4
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• pp.65-76
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• 2011

Objectives : The aim of this study is to survey adverse events of acupuncture in the course of peripheral facial palsy treatments. Methods : The prospective observational study included patients hospitalized to receive acupuncture treatments for peripheral facial palsy. All patients were surveyed daily for adverse events associated with the acupuncture treatments applied the day before. Results : A total of 50 patients received $37.68{\pm}20.37$ acupuncture treatments in average. 36 patients reported experiencing at least one adverse event. Total 163 adverse events were reported. Common adverse events were bleeding(62), bruise(33), pain(30), tiredness(11), hematoma(7). The others were paresthesia(4), erythema(3), delay of removing needles(2), edema(2), aggravation of symptoms(2), declining of appetite(2), pruritus(1), drowsiness(1), anxiety(1), sweating(1), headache(1). Conclusions : Acupuncture has adverse events, like any therapeutic approaches. However, it is a relatively safe treatment. In future study, organized large scale prospective survey should be carried out to fully prove that acupuncture is safe.