• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2003년도 제120회 추계학술대회 제 5차 한ㆍ일 치과보존학회 공동학술대회
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• pp.584-584
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• 2003

The aim of this study was to investigate the success rate of endodontic microsurgery. Three calibrated examiners evaluated a total of 51 teeth that were treated with endodontic microsurgery in Samsung Medical Center, Seoul, Korea, during the period of 1996-2000. The periapical radiographs were evaluated and compared with the preoperative and follow-up radiographs. In addition, a standardized questionable was used to assess the clinical status of each tooth. The data was integrated and each case was categorized as being a clinical success, questionable success or a failure.(omitted)

• 정보보호학회논문지
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• 제17권1호
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• pp.3-10
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• 2007

본 논문에서는 ICISC 2004에 제안된 status certificate-based encryption(SCBE) 기법과 EUC Workshops 2006에 제안된 certificateless signature(CLS) 기법의 취약성을 제시한다. 이 두 기법은 ID 기반 암호시스템이 가지는 키 위탁 성질을 없애기 위해 사용자와 관리서버(CA또는 KGC)가 함께 사용자 키(SCBE: 복호용 키, CLS: 서명용 키)를 생성하게 되어 있고, 이들 중 한쪽만을 제어할 수 있는 공격자는 암호시스템에 위해를 가할 수 있는 어떠한 이점도 가질 수 없다고 알려져 있다. 그러나, 본 논문에서는 각 기법의 공격모델에서 허용하는 공격자가 공개키 변조를 통해 독자적으로 사용자 키를 생성하여 사용할 수 있음을 보인다.

• 한국방재학회 논문집
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• 제3권3호
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• pp.125-132
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• 2003

본 연구에서는 국지성 이상호우에 대한 하천의 치수 안전도를 분석하기 위하며 서울의 도시하천중 지방2급 하천인 홍제천을 연구 대상하천으로 선정하였고, 최근 발생한 이상호우 중에서 서울에 많은 피해를 입힌 2001년 7월 14일${\sim}$15일 강우 310.1mm와 서울지역 가능최대강수량(PMP)740.0mm를 적용 강우로 선정하였다. 홍제천의 치수 안전도를 분석한 결과, 계획빈도인 50년인 경우, 안전단면이 85%, 관리단면이 15%, 위험단면은 발생치 않았으나, 100년 빈도에서 안전단면이 5% 감소하고 관리단면이 4% 위험단면은 2% 증가하였다. 빈도가 증가함에 따라 증감율은 작게 나타났고 200년 빈도 이후에는 관리단면은 거의 변화가 없었으며 위험단면이 다소 증가하여 300년 빈도에서는 50년 빈도에 비해 8%, 500년 빈도에서는 12% 증가하였고 1000년 빈도에서는 위험단면이 19%, 관리단면이 13% 크게 증가하였다.

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.423-428
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• 1997

Vibrio vulnificus is a halophilic gram-negative human pathogen, which affects people with underlying liver diseases or a suppressed immune system, often leading to primary septicemia with a mortality rate of higher than 60%. In an effort to develop an oral vaccine against V. vulnificus infection, we prepared a whole cell killed vaccine of V. vulnificus on a large scale and compared the immunogenicity and protective efficacy of the vaccine administered in three formulation forms in rabbits. Since V. vulnificus O-antigen serotypes 1, 2, 3, 4, 5, and 7 account for more than 95% of clinical isolates, we prepared cell lysates from these six serotype strains and mixed in equal amounts for a vaccine. The vaccine was administered to rabbits intramuscularly (i.m.), orally as granules or as enteric-coated granules. In rabbits, all three formulation forms elicited a high level of serum IgG antibody reactive not only to the six strains but also to other O-antigen serotypes 6, 8 and 9, indicating cross-reactivities among the strains. Immunotherapeutic efficacy of the antisera was also evaluated by a passive immunization assay, which revealed that the orally immunized antisera as well as the i.m. immunized antisera was protective against a subsequent lethal challenge of V. vulnificus. These data demonstrate that oral immunization with a V. vulnificus whole cell lysate vaccine induced a systemic immune response and suggest the feasibility of development of this vaccine preparation as an oral vaccine.

• Journal of Microbiology and Biotechnology
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• 제7권2호
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• pp.144-150
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• 1997

We developed a simple and efficient method to prepare a Pseudomonas vaccine of outer membrane (OM) proteins free from lipopolysaccharide (LPS). A three step purification process including extraction, ultrafiltration and ultracentrifugation effectively removed LPS from the OM protein fraction. Approximately 2 mg of the OM proteins was obtained from 1 g of wet cell. LPS contaminant in the vaccine preparation was less than 0.003% (w/w) of protein and protease activity was not detectable. To achieve a wide range of protection, OM proteins prepared from four attenuated P. aeruginosa strains were mixed in equal amounts and used as a vaccine, which elicited in rabbits a high titer of antibody reactive to all of the seven Fisher types. The antisera from the immunized rabbit had a strong reactivity to vaccine proteins larger than 25 kDa. In a burned mouse infection model, immunization with the vaccine significantly enhanced bacterial clearance in the Pseudomonas infected skin. The vaccination also provided mice an excellent protection against Pseudomonas infection (11, 16). Data on antigenicity, mutagenicity, acute, subacute toxicity and pharmacological tests confirmed the safety of the vaccine (1, 3, 10, 12, 17). These data demonstrate that this method can be applied to manufacture a bacterial vaccine of OM proteins with safety and prophylactic efficacy at a practical low cost.

• 청정기술
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• 제8권4호
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• pp.223-228
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• 2002

다양한 화학구조를 포함하는 폴리에스테르를 분해성 수지인 셀룰로오스와 비분해성 수지인 PVC 및 PE를 기준물질로 한 controlled compost test와 soil burial test를 적용하여 생분해 거동을 살펴보았다. 이들 고분자의 화학구조가 유연한 지방족 사슬과 에스테르기를 가지고 있는 지방족 폴리에스테르는 가수분해 효소에 의해 분해되고, 방향족 고리를 포함하는 공중합 형태의 폴리에스테르는 상대적으로 낮은 생분해도를 보여주었다. 조절된 퇴비화 조건에서 셀룰로오스가 45일에 70.6%의 분해도를 보일 때, 합성된 poly(butylene adipate-co-succinate), poly(butylene succinate), poly(butylene adipate-co-succinate-co-terephthalate)는 각각 44.0%, 32.0%, 23.4%,의 생분해도를 보여주어 이들 고분자의 화학적 구조에 따라 생분해거동이 많은 영향을 받음을 확인하였다.

• 동의신경정신과학회지
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• 제20권3호
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• pp.15-33
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• 2009

Objectives : To study for classifying indications and procedure of historical changes, as a preliminary study, we collected and arranged prescriptions of headaches in medical documents after "Hwang-Je-Nae-Gyeong". Methods : 1. The researcher reviewed books, as a bibliography, recording to associate with a headaches in "UI-Bu-Jeon-Rok". 2. In reference books, we collected and arranged the herb medicines related to headaches in chapter of them. 3. In case of no chapter associated with headaches in books, we found prescriptions related to headaches and rearranged them. 4. Applied or added herb medicines were excluded, if the chief virtue was not primarily for headaches. 5. Prescriptions for signs to headaches related to dizziness were excluded. 6. Prescriptions for signs to associated with tremor were excluded. 7. We excluded prescriptions of headaches for dermatological disease. Results and conclusions : 1. Thirty seven documents were referred to the section of a headche in "UI-Bu-Jeon-Rok". 2. Prescriptions of "Sang-Han-Ron", which were related to headaches, were repeatedly quoted in chinese medical publications. 3. Nameless prescriptions of headaches were recorded from earlier books to later publications. 4. It started to record prescriptions of headaches separately from "Hawl-In-Seo" in 1107. 5. Described how to discriminate Naesang from Oegam, but prescriptions of headache were not listed in Dongwon Yi's "Nae-Oe-Sang-Byeon-Hok-Ron" which was published in 1247.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1232-1238
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• 2004

To investigate the production and characteristics of thermostable levan sucrase from Rahnella aquatilis ATCC 33071, the levan sucrase gene from R. aquatilis was cloned and expressed in Escherichia coli without induction system. Expression of levansucrase gene in E. coli had no notable or detrimental effect on the growth of host strain, and the recombinant levan sucrase exhibited levan synthesis activity. Levansucrase was secreted to the periplasm in E. coli, and addition of $0.5\%$ glycine yielded further secretion of levansucrase to the growth medium and resulted in an increase of total levansucrase activity. Furthermore, the cellular levansucrase was evaluated for the production of levan by using toluene­permeabilized whole-cells. The levansucrase was thermostable at $37^{\circ}C$. The molecular size oflevan was $1{\times}\;10^{6}$ Da, as determined by HPLC, and the degree of polymerization of levan varied with incubation temperatures: Low incubation temperature was preferable for the production of high-molecular size levan. The present study demonstrated that the mass production of levan and levan oligosaccharides can be achieved by glycine supplementation to the growth medium or by toluene­permeabilized whole-cells.

• 한방안이비인후피부과학회지
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• 제24권1호
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• pp.64-77
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• 2011

Objectives : 판람근(板藍根)은 십자화과에 속하는 대청(大靑) 또는 숭남의 근(根)을 건조한 것이다. 본 연구는 판람근(板藍根)이 청열해독(淸熱解毒)함에 근거하여, LPS로 활성화된 Raw264.7 cell에서 판람근(板藍根)과 그 성분중의 하나인 tryptanthrin이 염증매개물질에 미치는 효과를 살펴보고자 하였다. Methods : 세포생존율은 MTT, nitric oxide (NO)는 Griess reagent를 사용하여 측정하였으며, 각 단백질의 발현량은 Western blot 방법을 사용하였으며, cytokine 및 cyclooxygenase-2 (COX-2)는 ELISA방법을 사용하여 측정하였다. Results : LPS는 NO 및 prostaglandin E2 (PGE2)를 유의하게 상승시켰으며, 판람근(板藍根)추출물 (IRE) 및 tryptanthrin 은 이들을 유의하게 억제하였다. 그러나 판람근(板藍根)의 또 다른 성분인 indigo는 유의한 결과를 나타내지 못하였다. IRE와 tryptanthrin은 inhibitory kappa B alpha의 인산화를 억제하여, nuclear factor-${\kappa}$B (NF-${\kappa}$B)의 핵으로의 전위(轉位)를 억제하여, iNOS 및 cytokine을 억제하였다. IRE와 tryptanthrin의 PGE2 억제는, COX-2의 발현억제에서가 아니라, COX-2의 활성을 억제함에서 기인하였다. Conclusion : 이러한 결과는 판람근(板藍根)이 NF-${\kappa}$B pathway를 경유하여 iNOS의 발현 및 COX-2의 활성을 억제함을 나타내며, 이러한 판람근(板藍根)의 항염증효능은 일부 tryptanthrin의 작용에서 기인함을 시사한다.

• 한국광학회지
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• 제26권4호
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• pp.195-202
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• 2015

기층의 잔류 이방성에 영향을 받지 않고 러빙된 폴리이미드 배향막의 초미세 광학 이방성을 정밀하게 측정할 수 있는 반사형 타원계를 개발하고 러빙의 세기를 달리하여 제작한 배향막의 광학이방성을 이 반사형 타원계를 사용하여 측정, 분석하였다. 투과형 타원계를 사용하여 측정한 리타데이션과 비교하여 개발된 반사형 타원계의 신뢰도 및 광학 이방성의 측정 재현성을 확인하고 배향막의 형성 여부와 배향막의 형성정도를 정량적으로 평가하였다