• Journal of vocational education research
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• v.37 no.4
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• pp.67-89
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• 2018

The purpose of this study is to analyze the role of career flexibility on employment status, career growth, and job satisfaction longitudinally. Demographic information(gender, age, parent's income level, and students' perceived financial states of family), vocational identity, employment status, career growth, and job satisfaction at 1, 2, and 3 years after the first data collection with a sample of 259(time 1), 229(time 2), 182(time 3) were collected respectively. Multivariate regression and logistic regression analysis were performed. Results showed that in-breadth career exploration positively predicted to employment status at time 1, 2, 3 consistently and career flexibility also positively predicted to employment status at time 2, 3. The longitudinal effect of career flexibility was found that the expectancy of career flexibility had shown to have stronger effect. In addition, career growth was influenced by in-depth career exploration at time 2, 3. On the other hand, no variables were related to job satisfaction in this study. Results of the current study provided the new insights for career education and limitation and future suggestions were discussed.

• Genes and Genomics
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• v.40 no.12
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• pp.1351-1361
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• 2018

A new cold tolerant germplasm resource named glutinous rice 89-1 (Gr89-1, Oryza sativa L.) can overwinter using axillary buds, with these buds being ratooned the following year. The overwintering seedling rate (OSR) is an important factor for evaluating cold tolerance. Many quantitative trait loci (QTLs) controlling cold tolerance at different growth stages in rice have been identified, with some of these QTLs being successfully cloned. However, no QTLs conferring to the OSR trait have been located in the perennial O. sativa L. To identify QTLs associated with OSR and to evaluate cold tolerance. 286 $F_{12}$ recombinant inbred lines (RILs) derived from a cross between the cold tolerant variety Gr89-1 and cold sensitive variety Shuhui527 (SH527) were used. A total of 198 polymorphic simple sequence repeat (SSR) markers that were distributed uniformly on 12 chromosomes were used to construct the linkage map. The gene ontology (GO) annotation of the major QTL was performed through the rice genome annotation project system. Three main-effect QTLs (qOSR2, qOSR3, and qOSR8) were detected and mapped on chromosomes 2, 3, and 8, respectively. These QTLs were located in the interval of RM14208 (35,160,202 base pairs (bp))-RM208 (35,520,147 bp), RM218 (8,375,236 bp)-RM232 (9,755,778 bp), and RM5891 (24,626,930 bp)-RM23608 (25,355,519 bp), and explained 19.6%, 9.3%, and 11.8% of the phenotypic variations, respectively. The qOSR2 QTL displayed the largest effect, with a logarithm of odds score (LOD) of 5.5. A total of 47 candidate genes on the qOSR2 locus were associated with 219 GO terms. Among these candidate genes, 11 were related to cell membrane, 7 were associated with cold stress, and 3 were involved in response to stress and biotic stimulus. OsPIP1;3 was the only one candidate gene related to stress, biotic stimulus, cold stress, and encoding a cell membrane protein. After QTL mapping, a total of three main-effect QTLs-qOSR2, qOSR3, and qOSR8-were detected on chromosomes 2, 3, and 8, respectively. Among these, qOSR2 explained the highest phenotypic variance. All the QTLs elite traits come from the cold resistance parent Gr89-1. OsPIP1;3 might be a candidate gene of qOSR2.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.226-230
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• 2010

'Gwanghan' (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2009. It was derived from a cross between 'Early80' and 'Gwiri23'. A parent, 'Early80', has early heading and high yielding, while Gwiri23 has mid-heading with large-size grain. Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon and Yeoncheon, respectively. A line, 'SO96016-B-112-YB-12-7', was selected for earliness, cold tolerance, and good agronomic characteristics and designated as a line name of 'Gwiri64'. The line was subsequently evaluated for cold tolerance, earliness, and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2007 to 2009, and finally named as 'Gwanghan'. Over 3 years, the average forage dry matter yield of 'Gwanghan' harvested at milk-ripe stage was $16.4MT\;ha^{-1}$, compared with $15.4MT\;ha^{-1}$ of check cultivar 'Samhan'. 'Gwanghan's' heading date is about 3 days earlier than that of check cultivar, and is adapted primarily for winter cultivation in the mid-southern regions of Korean peninsula.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.654-658
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• 2010

A FA (Lilium formolongi $\times$ Lilium asiatic hybrid) intersectional hybrid lily cultivar 'Orange Crown' was developed in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The cross and immature embryo rescue was conducted between female parents 'Migreen' (Lilium formolongi 'Raizan' $\times$ Lilium asiatic hybrid 'A61') and male parent 'A01-187' (L. asiatic 'A96-28' $\times$ 'Sanzio') by cut style pollination method at Suwon in 2001. It was preliminarily selected as 'FA04-27' in 2004. Multiplication, bulb production and characteristic tests were conducted from 2004 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS at Taean in 2007. 'Orange Crown' flowers in the late of June and grows more than 183 cm stem length. Flowers bloom upward-facing, a little spotted and orange (RHS, O24A) petals. The pollen of 'Orange Crown' was sterile.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.100-108
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• 2010

This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.218-221
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• 2010

'Anyu', a perilla (Perilla frutescens (L.) Britton) cultivar for edible seed was developed by the Department of Functional Crop Science, National Institute of Crop Science, RDA in 2008. It was originated from the cross between a pedigree of YCPL1824 as a female and SF9225-3-1 as a male parent in 1998. 'Anyu' can be characterized by purple flower color and brown grain color. 'Anyu' showed semi-dwarft type with 120 cm height, and has lodging tolerance. Maturing date of 'Anyu' was September 24, which is 15 days faster than that of 'Saeyeopsil'. The early maturity of this new cultivar showed a great advantage to various planting system with other crops. This new cultivar has high oil content (45%) and high linolenic acid in the fatty acid composition. The yield potential of 'Anyu' was about 0.9 ton/1ha in the regional yield trial.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.496-501
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• 2009

In order to promote the efficiency of strawberry breeding programs, pollen viability of strawberry, 'Seolhyang' was investigated using the fluorochromatic reaction test and seed set under controlled environment. Pistil receptivity was also assessed by hand pollination. Four varieties including 'Maehyang' were used for the test of pistil receptivity with 'Seolhyang' as a pollen parent. Pollen viability remained high for several days under dry conditions as below 33% relative humidity while the greatest loss of viability occurred at 76% relative humidity. The viability was rapidly decreased at high humidity and almost all grains were unviable in 7 days after storage. Pollen viability does not appear to be drastically reduced if the relative humidity is low. Therefore, humidity is more important factor than temperature for the pollen viability in Fragaria${\times}$ananassa. The rate of seed set by hand pollination lasted higher than the average of 77.2% from 2 to 8 days after emasculation when the daily average temperature was around $15^{\circ}C$ in plastic house. It began to decline gradually from 10 days and had decreased dramatically after 12 days except several cultivars. Based on the daily mean accumulated temperature, it is recommended to have an artificial pollination between the range of $45{\sim}140^{\circ}C$ after the emasculation to increase the rate of seed set in strawberry.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.210-213
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• 2011

A dark pink semi-double freesia (Freesia hybrida Hort.) 'Happy Birthday' was bred from a cross between 'Pink Jewel', F1 progeny of 'Michelle' and 'Rossini' as a female and 'White Lace' as a male parent. Test for the growth vigor and flowering characteristic were conducted from 2002 to 2007. A new variety 'Happy Birthday' has semi-double flower type with dark pink color (RHS color chart #RP66C). It has multiple flowers, distinctive flower shape, upright leaves and also stalks. The average yield of this variety 'Happy Birthday' counted over 6.7 stems per plant per season in the standard greenhouse cultivation method. It has also good production of cormlets, vigorous growth. A new variety 'Happy Birthday' is suitable for the greenhouse conditions during winter season in Korea.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.502-506
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• 2009

"Hongyoung" was bred within the potato breeding program of National Institute of Highland Agriculture (NIHA). It was originated from a cross made between Atlantic as a female and AG34314 as a male parent in 2003. "Hongyoung" was evaluated as a clone number Daegwan 1-102 for the growth and tuber characteristics, yielding ability, and resistance to major diseases in the regional yield trials from 2006 to 2007. Finally, this clone was renamed as "Hongyoung" and was presented to Korean Seed & Variety Service in 2007 for registration as a new potato cultivar. "Hongyoung" has a round shape, shallow eye depth, red skinned and fleshed tuber characteristics. Its mean tuber yields from the regional yield trials was 37.4 MT/ha, and it showed the highest yield in spring cropping. "Hongyoung" showed high resistance to common scab (Streptomyces scabies) and potato leaf roll virus (PLRV), however it was susceptible to both potato virus Y (PVY) and late blight (Phytophthora infestans). The dry matter content of "Hongyoung" was low to medium 17.7%, therefore it is suitable for table use. Its anthocyanin content was the highest in autumn cropping with 31.8 mg/100g fresh weight and it contained 3.6 times higher anthocyanin than the control, Jasim.

• Journal of Life Science
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• v.29 no.3
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• pp.376-381
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• 2019

To construct useful yeast strain for bioethanol production, we improved yeast harboring various phenotypes by using yeast protoplast fusion method. In this study, S. cerevisiae BYK-F11 strain which have ethanol tolerance, thermotolerance and ${\beta}-glucanase$ activity and P. $stipitis{\Delta}ura$ strain which has xylose metabolism pathway were fused by genome shuffling. P. $stipitis{\Delta}ura$ strain was constructed for protoplast fusion by URA3 gene disruption, resulting in uracil auxotroph. By protoplast fusion, several fused cells were selected and BYKPS-F8 strain (fused cell) showing both karyotypes from two parent strains (S. cerevisiae BYK-F11 and P. $stipitis{\Delta}ura$ strain) among 22 fused cells was finally selected. Sequentially, various phenotypes such as ${\beta}-glucanase$ activity, xylose utility, ethanol tolerance, thermotolerance and ethanol productivity were analyzed. The BYKPS-F8 strain obtained ${\beta}-glucanase$ activity from BYK-F11 strain and 1.2 fold increased xylose utility from P. $stipitis{\Delta}ura$ strain. Also, the BYKPS-F8 strain showed thermotolerance at $40^{\circ}C$ and increased ethanol tolerance in medium containing 8% ethanol. In this fused cell, 7.5 g/l ethanol from 20 g/l xylose was produced and the multiple phenotypes were stably remained during long term cultivation (260 hr). It was proved that novel biological system (yeast strains) is easily and efficiently bred by protoplast fusion among yeasts having different genus.