• Reproductive and Developmental Biology
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• 제30권3호
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• pp.157-162
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• 2006

본 연구에서는 인간 부자상선 호르몬의 발현을 유도적으로 조절할 수 있는 retrocvirus vector system을 확립하고자 하였다. 이에 tetracycline계 물질로 발현을 유도적으로 조절할 수 있는 one vector 형태의 Tet-On system을 이용하였으며 WPRE 서열을 도입하여 유도적 조건에서 외래 유전자의 발현을 증가시켰다. 구축한 각각의 표적세포에서 RT-PCR과 ELISA를 이용하여 hPTH유전자의 발현 정도를 비교 측정한 결과, WPRE가 hPTH의 3' 위치에 도입된 $RevTRE-PTH-WPRE-CMVp-rtTA2^sM2$ virus를 이용하여 유전자를 전이시킨 경우에 hPTH의 발현량이 가장 높은 것으로 나타났고, 또한 유도율도 가장 큰 것으로 확인되었다. 이 system을 이용하여 생산한 고감염가의 virus는 인간의 부갑상선 호르몬을 생산하기 위한 동물세포주의 구축이나 형질전환 동물의 생산에 있어서 매우 효율적인 유전자 전이 수단이 될 것으로 사료된다.

• 한국산학기술학회논문지
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• 제16권6호
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• pp.3882-3888
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• 2015

본 연구는 국가자료인 2011년도 국민건강영양조사 자료를 이용하여 50세 이상 성인에서 비만이 PTH에 미치는 영향을 평가하고자 실시하였다. 조사 참여자수는 8,518명이었고, 이중 50세 이상 성인 3,305명을 최종분석 대상자로 하였다. 본 연구의 주요결과는 PTH 평균값에 영향을 주는 관련변수(연령, 성별, SBP, DBP, 25(OH)D)를 보정 후, 정상군 (BMI, < $23.0kg/m^2$; $66.25{\pm}0.89ng/dL$)에 비하여 과체중군(BMI, $23.0-24.9kg/m^2$; $67.83{\pm}1.07 ng/dL$), 비만군(BMI,${\geq}25.0kg/m^2$; $70.71{\pm}0.94ng/dL$)의 PTH 평균값이 유의하게 증가하였고(p=0.003), Non-abdominal obesity군($66.63{\pm}0.74ng/dL$)에 비하여 Abdominal obesity군($69.30{\pm}0.80ng/dL$)의 PTH 평균값이 증가하였다(p=0.017). 결론적으로 비만과 복부비만은 PTH의 증가와 관련이 있다.

• 대한정형외과학회지
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• 제55권3호
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• pp.237-243
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• 2020

목적: 고령 여성에서 대퇴부에 발생한 골다공증과 동반된 전자간 골절에서 수술 후에 부갑상선 호르몬 제제 투여 후 골절 치유에 대한 영향을 알아보고자 하였다. 대상 및 방법: 2013년 7월부터 2017년 12월까지 대퇴부 전자간 골절 및 골다공증을 진단 받은 후 수술을 시행한 65세 이상의 여자 환자에서 부갑상선 호르몬 제제를 투여한 군과 투여하지 않은 군을 대상으로 최초 가골의 형성 시기, 가골교로의 진행 시기 및 골유합 진행 시기를 조사하였다. 결과: 부갑상선 호르몬 투여군에서 최초 가골의 형성은 평균 32일, 가골교로의 진행은 평균 58일, 골유합이 된 시기는 평균 83일로 투약을 하지 않은 군에 비하여 통계적으로 의미 있게 기간이 단축된 소견을 보였다. 결론: 골다공증 치료제인 부갑상선 호르몬 제제는 고령의 여성에서 골다공증이 동반된 전자간 골절에서 가골의 형성 및 치유 과정을 촉진시킨다. 따라서 수술 후 이의 투여가 도움이 될 거라 생각된다.

• Journal of Nutrition and Health
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• 제40권4호
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• pp.320-326
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• 2007

An important related question is whether arginine has influence bone metabolism. The effect of arginine supplements on bone markers and related hormones were studied in young female Sprague-Dawley rats fed either an arginine supplemented diet or control diet. Twenty four rats (body weight 83${\pm}$5 g) were randomly assigned to one of two groups, consuming casein or casein with supplemented arginine diet. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. Bone formation was measured by serum osteocalcin and alkaline phosphatase (ALP) concentrations. And bone resorption rate was measured by deoxypyridinoline (DPD) crosslinks immunoassay and corrected for creatinine. Serum osteocalcin, growth hormone, estrogen, insulin-like growth factor-1 (IGF-1), parathyroid hormone (PTH) and calcitonin were analyzed using radioimmunoassay kits. The weight gain and mean food intake were not affected regardless of diets. The rats fed arginine-supplemented diet had not significantly different in ALP, osteocalcin, crosslinks value, PTH, estradiol, and IGF-1 compared to those fed casein diet group. The arginine-supplemented group had significantly higher growth hormone and calcitonin than casein group. This study suggests that arginine is beneficial for bone formation in growing female rats. Therefore exposure to diet which rich in arginine early in life may have benefits for bone formation and osteoporosis prevention.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.220.2-220.2
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• 2002

• Annals of Clinical Neurophysiology
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• 제23권2호
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• pp.134-137
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• 2021

Primary hyperparathyroidism (PHP) is a disease in which excessive amounts of parathyroid hormone (PTH) are secreted and calcium levels in the blood increase. Hypercalcemia caused by PHP has a major influence on the peripheral nervous system and produces symptoms such as muscle cramps, paresthesia, and proximal muscle weakness. Here we report a rare case of sensory-dominant polyneuropathy caused by PHP, which improved after surgery.

• Nutritional Sciences
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• 제8권2호
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• pp.111-117
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• 2005

It has been more than three decades since the first assay assessing circulating 25 (OH)D in human subjects was performed That publication as well as several that followed it defined 'normal' nutritional vitamin D status in human populations. Recently, the wisdom by which 'normal' circulating 25 (OH)D levels in human subjects were assigned in the past has come under question. It appears that sampling human subjects, who appear to be free from disease, and assessing 'normal' circulating 25 (OH)D levels by plotting a Gaussian distribution is grossly inaccurate. There are many reasons why this method is inaccurate, including race, lifestyle habits, sunscreen usage, age, latitude, and inappropriately low dietary recommendations for vitamin D. For instance, a 400 IU/day. AI for vitamin D is insignificant when one considers that a 10-15 minute whole body exposure to peak summer sun will generate and release up to 20,000 IU vitamin $D_3$ into the circulation. Recent studies, which orally administered up to 10,000 IU/day vitamin $D_3$ to human subjects for several months, have successfully elevated circulating 25 (OH)D levels to those observed in individuals from sun-rich environments. Further, we are now able to accurately assess sufficient circulating 25 (OH)D levels utilizing specific biomarkers instead of guessing what an adequate level is. These biomarkers include intact parathyroid hormone (PTH), calcium absorption, bone mineral density (BMD), insulin resistance and pancreatic beta cell function. Using the data from these biomarkers, vitamin D deficiency should be defined as circulating levels of 25 (OH)D$\leq$30 ng/mL. In certain cases, such as pregnancy and lactation, significantly higher circulating 25 (OH)D levels would almost certainly be beneficial to both the mother and recipient fetus/infant.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2004년도 추계학술대회
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• pp.22-33
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• 2004

It has been more than three decades since the first assay assessing circulating 25(OH)D in human subjects was performed. That publication as well as several that followed it defined 'normal' nutritional vitamin D status in human populations. Recently, the wisdom by which 'normal' circulating 25(OH)D levels in human subjects were assigned in the past has come under question. It appears that sampling human subjects, who appear to be free from disease, and assessing 'normal' circulating 25(OH)D levels by plotting a Gaussian distribution is grossly inaccurate. There are many reasons why this method is inaccurate, including race, lifestyle habits, sunscreen usage, age, latitude, and inappropriately low dietary recommendations for vitamin D. For instance, a 400IU/day. AI for vitamin D is insignificant when one considers that a 10-15 minute whole body exposure to peak summer sun will generate and release up to 20,000 IU vitamin $D_3$ into the circulation. Recent studies, which orally administered up to 10,000 IU/day vitamin $D_3$ to human subjects for several months, have successfully elevated circulating 25(OH)D levels to those observed in individuals from sun-rich environments. Further, we are now able to accurately assess sufficient circulating 25(OH)D levels utilizing specific biomarkers instead of guessing what an adequate level is. These biomarkers include intact parathyroid hormone (PTH), calcium absorption, bone mineral density (BMD), insulin resistance and pancreatic beta cell function. Using the data from these biomarkers, vitamin D deficiency should be defined as circulating levels of $25(OH)D{\leq}30ng/mL$. In certain cases, such as pregnancy and lactation, significantly higher circulating 25(OH)D levels would almost certainly be beneficial to both the mother and recipient fetus/infant.

• Journal of Nutrition and Health
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• 제34권5호
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• pp.547-553
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• 2001

This study was designed to observe the change of body composition and nutrient metabolites and the excretion of minerals during complete fasting for 10 days in thirty women. Average loss of body weight was 7.98kg and body fat was gradually reduced after 9 days fasting, but the loss of lean body mass was reduced after 7 days fasting. Serum glucose level was sustained at constant level, but serum levels of blood urea nitrogen, free acid and $\beta$-hydroxybutyrate were significantly increased during fasting but decreased after re-feeding. Urinary excretions of 3-methylhistidine, total creatinine, and urea were high in the beginning of fasting but gradually decreased. Serum level of parathyroid hormone was significantly reduced by fasting but regained after re-feeding. Serum level of minerals was at the constant level throughout the experimental period. The urinary excretion of minerals(Ca, K, Mg, P) was significantly increased in the early stage of fasting and then reduced from 7 days, but the excretion of Zn was continuously increased until the late stage of fasting. These results showed that amino acid fatty acid released from the breakdown of muscle protein and body fat, respectively, were utilized for energy during fasting. Body weight and BMI were reduced because of the increased muscle protein breakdown and body water excretion during early stage of fasting, but the significant body fat loss was after 9 days fasting. Therefore, it could be suggested to fast for longer than 10 days if the reduction of body fat was planned by fasting, and recommed to exercise and ingest more vitamins and minerals to replace the excreted minerals during fasting. (Korean J Nutrition 34(5) : 547~553, 2001)

• 대한두경부종양학회지
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• 제14권2호
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• pp.206-213
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• 1998

Primary hyperparathyroidism is still uncommomn disease in Korea. However the frequency of this disease has been slowly increased with routine measurement of serum calcium and increasing awareness of hyperparathyroidism in recent years. The diagnosis is established by a persistent elevation of serum calcium and parathyroid hormone and by clinical evaluation. This is a report of eleven patients with primary hyperparathyroidism treated with surgical operation during a period from 1983 to 1997 at Department of Hospital. Authors analyzed the cases to evaluate clinical characteristics and outcome of surgical treatment retrospectively. The result was as follows. 1) In sex distribution, female patients were eight and three were male, the age distribution ranged from 18 to 67 years. 2) The presenting clinical manifestations were renal and urinary stone in eight, bone pain or fracture in six, muscle weakness in four, neurologic symptoms in four, neck mass in three, hypertension in two, and G-I symptoms in one. 3) All patients showed hypercalcemia and elevated serum parathyroid hormone level. 4) Preoperative localization study was performed with computerized tomography, ultrasonography, MRI, arteriography and thyroid scaning. 5) The tumor locations were left lower in eight, left upper in one, right lower in one, and right upper location was one case. 6) Histopathologic findings disclosed adenoma in all cases. 7) All patients were treated by surgical excision and postoperatively transient hypocalcemia occurred in six patients, but no other complication was developed.