• 한국균학회지
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• 제48권4호
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• pp.423-443
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• 2020

Freshwater fungi are a poly-phylogenetic group of taxonomically diverse organisms. In this study, we isolated diverse fungal strains from various environmental samples obtained from freshwaters in Korea. These strains were identified by performing molecular phylogenetic analyses of rDNA and/or other sequences (beta-tubulin, RNA polymerase II, and translation elongation factor 1). In addition, we examined their morphological characteristics microscopically and cultural characteristics using different media. We identified eleven unrecorded Pezizomycotina species: Cladosporium angulosum, Pseudorobillarda phragmitis, Paraconiothyrium estuarinum, Pseudopithomyces palmicola, Pyrenochaetopsis paucisetosa, Thelebolus globosus, Plagiostoma mejianum, Trichoderma cremeum, Fusarium tanahbumbuense, Coniochaeta endophytica, and Chaetomium tenue. Environmental samples obtained from different freshwater ecosystems in Korea could thus be a good source for isolating and investigating novel fungal species.

• 한국균학회지
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• 제45권4호
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• pp.292-303
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• 2017

2016년 버섯 재배사 내 실내공기, 식물병반(동백나무의 잎, 줄딸기, 은방울꽃, 생강나무), 버섯 배양배지 재료, 오염된 가구 등에서 진균을 분리 동정하였다. 동정된 진균 중 18종이 국내 미기록임을 확인하였다. 동정된 진균은 버섯 재배사 내 실내공기에서 6종, 식물병반에서 6종, 버섯 배양배지 재료에서 5종, 오염된 가구에서 1종이 각각 분리되었다 이들 동정된 균주에 대한 형태학적 특성과 ITS rDNA, 18S rDNA, 28S rDNA, calmodulin gene, translation elongation factor gene, ${\beta}-tubulin$ gene 등의 염기서열에 기반한 분자계통학적 관계에 대하여 기술하였다.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1526-1532
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• 2021

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA₁ 2.487 ng/ml, GA₃ 2.592 ng/ml, GA₉ 3.998, and GA₂₄ 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA₉ and GA₂₄ than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.