• Title/Summary/Keyword: ParA

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Characterization of Quinolone-Resistant Clinical Isolates of Escherichia coil in Korea

  • Lee, Yeonhee;Yoojung Oh;Park, Seohyung;Misun Ha
    • Journal of Microbiology
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    • v.40 no.2
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    • pp.98-103
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    • 2002
  • Twenty-eight clinical isolates of Escherichia coil, composed of thirteen norfloxacin resistant isolates (MIC of >16${\mu}$g/ml), one intermediately resistant isolate (MIC of 8${\mu}$g/ml), and fourteen susceptible isolates (MIC of <4${\mu}$g/ml), were randomly selected to study the norfloxacin resistance mechanism and phylogeny in clinical isolates in Korea. Eleven nofloxacin resistant isolates and one susceptible isolate were multi-drug resistant (MDR). Every norfloxacin resistant isolate with MIC higher than 32${\mu}$g/ml had the same three mutations: Ser83\longrightarrowLeu and Asp87\longrightarrowAsn or Tyr in GyrA and Ser80\longrightarrowIle in ParC. Whereas a resistant isolate with MIC of 16${\mu}$g/ml had three mutations but Asp87 in GyrA was replaced with Gly instead of Asn. The intermediately resistant isolate had the same two mutations in GyrA but a different mutation in ParC, Glu84\longrightarrowLys. Among the susceptible isolates, two isolates with MIC of 4${\mu}$g/ml had one mutation: Ser83\longrightarrowiLeu in GyrA, and no mutation was found in the susceptible isolates. Resistant isolates showed higher efflux activity than the susceptible ones, with random amplification of polymorphic DNA (RAPD), six susceptible isolates form a separate group from the rest of the isolates.

Changes in Radiation Use Efficiency of Rice Canopies under Different Nitrogen Nutrition Status (질소영양 상태에 따른 벼 군락의 광 이용효율 변화)

  • Lee Dong-Yun;Kim Min-Ho;Lee Kyu-Jong;Lee Byun-Woo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.8 no.3
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    • pp.190-198
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    • 2006
  • Radiation use efficiency (RUE), the amount of biomass produced per unit intercepted photosynthetically active radiation (PAR), constitutes a main part of crop growth simulation models. The objective of the present study was to evaluate the variation of RUE of rice plants under various nitrogen nutritive conditions. from 1998 to 2000, shoot dry weight (DW), intercepted PAR of rice canopies, and nitrogen nutritive status were measured in various nitrogen fertilization regimes using japonica and Tongil-type varieties. These data were used for estimating the average RUEs before heading and the relationship between RUE and the nitrogen nutritive status. The canopy extinction coefficient (K) increased with the growth of rice until maximum tillering stage and maintained constant at about 0.4 from maximum tillering to heading stage, rapidly increasing again after heading stage. The DW growth revealed significant linear correlation with the cumulative PAR interception of the canopy, enabling the estimation of the average RUE before heading with the slopes of the regression lines. Average RUE tended to increase with the increased level of nitrogen fertilization. RUE increased approaching maximum as the nitrogen nutrition index (NNI) calculated by the ratio of actual shoot N concentration to the critical N concentration for the maximum growth at any growth stage and the specific leaf nitrogen $(SLN;\;g/m^2\;leaf\;area)$ increased. This relationship between RUE (g/MJ of PAR) and N nutritive status was expressed well by the following exponential functions: $$RUE=3.13\{1-exp(-4.33NNNI+1.26)\}$$ $$RUE=3.17\{1-exp(-1.33SLN+0.04)\}$$ The above equations explained, respectively, about 80% and 75% of the average RUE variation due to varying nitrogen nutritive status of rice plants. However, these equations would have some limitations if incorporated as a component model to simulate the rice growth as they are based on relationships averaged over the entire growth period before heading.

A Parallel Algorithm for Merging Relaxed Min-Max Heaps (Relaxed min-max 힙을 병합하는 병렬 알고리즘)

  • Min, Yong-Sik
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.5
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    • pp.1162-1171
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    • 1998
  • This paper presents a data structure that implements a mergable double-ended priority queue : namely an improved relaxed min-max-pair heap. By means of this new data structure, we suggest a parallel algorithm to merge priority queues organized in two relaxed heaps of different sizes, n and k, respectively. This new data-structure eliminates the blossomed tree and the lazying method used to merge the relaxed min-max heaps in [9]. As a result, employing max($2^{i-1}$,[(m+1/4)]) processors, this algorithm requires O(log(log(n/k))${\times}$log(n)) time. Also, on the MarPar machine, this method achieves a 35.205-fold speedup with 64 processors to merge 8 million data items which consist of two relaxed min-max heaps of different sizes.

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Antigenicity of Partial Fragments of Recombinant Pasteurella multocida Toxin

  • Lee, Jeong-Min;Woo, Hee-Jong
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1756-1763
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    • 2010
  • Pasteurella multocida serogroup D strain, which produces P. multocida toxin (PMT), is a widespread and harmful pathogen of respiratory diseases such as pneumonia and progressive atrophic rhinitis (PAR) in swine. Vaccination has been considered the most desirable and effective approach for controlling the diseases caused by toxigenic P. multocida. To investigate the antigenicity and immunogenicity of partial fragments of recombinant PMT, recombinant proteins of the N-terminal (PMT-A), middle (PMT-B), C-terminal (PMT-C), and middle-C-terminal (PMT2.3) regions of PMT were successfully produced in an Escherichia coli expression system. The molecular masses of PMT-A, PMT-B, PMT-C, and PMT2.3 were ca. 53, 55, 35, and 84 kDa, respectively, purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. All the recombinant proteins except for PMT-A showed immune responses to antisera obtained from a swine showing symptoms of PAR. Moreover, high titers of PMT-specific antibodies were raised from mice immunized with each of the recombinant proteins; however, the immunoreactivities of the antibodies to authentic PMT and heat-inactivated whole bacteria were different, respectively. In the protection study, the highest protection against homologous challenge was shown in the case of PMT2.3; relatively poor protections occurred for the other PMT fragments.

Changes of Chlorophyll Fluorescence and Photosynthesis under Different Shade Materials in Korean Ginseng(Panax ginseng C. A. Meyer) (해가림자재에 따른 인삼의 엽록소 형광 반응 및 광합성 변화)

  • Won, Jun-Yeon;Lee, Chung-Yeol;Oh, Dong-Joo;Kim, Sung-Man
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.6
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    • pp.416-420
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    • 2008
  • This study was conducted to investigate the influence of shading material on the chlorophyll fluorescence, photosynthesis, transpiration, stomatal conductance and its any correlations in Panax ginseng C.A.Meyer. Fo was higher in polyethylene shade net than in silver-coated shading plate, but this treatment caused a lower Fm in comparison with silver-coated shading plate. Also, Fv/Fm and PhiPS2 showed higher in silver-coated shading plate than in polyethylene shade net. The relationship between net photosynthetic rate and transpiration, stomatal conductance were increased as the PAR (Photosynthetic active radiation) was increased and reached maximum at the $200-400\;{\mu}mol/m^{2}/s$ of PAR in all of leaves, and the higher in silver-coated shading plate than in polyethylene shade net. A linear equation was obtained between net photosynthetic rate and transpiration, net photosynthetic rate and stomatal conductance. SPAD was higher in silver-coated shading plate than in polyethylene shade net.

Acanthoic acid blocks production of pro-inflammatory mediators by inhibiting the ERK activation in trypsin-stimulated human leukemic mast cells

  • Kang, Ok-Hwa;Tae, Jin;Choi, Yeon-A;Kwon, Dong-Yeul;Kim, Yun-Kyung;Cai, Xing-Fu;Kim, Young-Ho;Bae, Ki-Hwan;Lee, Young-Mi
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.88.3-89
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    • 2003
  • Acanthoic acid (AA) is a pimaradiene diterpene isolated from the Korean medicinal plant, Acanthopanax koreanum (Araliaceae), which has been traditionally used as a tonic and sedative as well as in the treatment of rheumatism and diabetes in korea. Proteinase-activated receptor-2 (PAR-2) agonist trypsin plays a role in inflammation, and human leukemic mast cells (HMC-l) express PAR-2. In the present study, the effect of acanthoic acid on production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and tryptase in trypsin-stimulated HMC-1 was examined. (omitted)

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Accumulation of Chlorogenic Acid as a near UV-shielding Compound in Cauliflower Grown under Enhanced UV-B Radiation

  • Shibata, Hitoshi;Tanaka, Tomoyuki;Yonemura, Takeshi;Sawa, Yoshihiro;Ishikawa, Takahiro
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.436-438
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    • 2002
  • Since solar radiation contains wavelength essential for photosynthesis accompanying with near-UV light, UV-B effects on biological parameters and acclimation mechanisms are influenced by photosynthetically active radiation (PAR). Therefore, to elucidate near-UV shielding mechanism in higher plants, we cultivated cauliflower under usual solar radiation and increased UV-B from fluorescent lamps, two- or three-fold excess over continuously estimated UV-B dose in PAR during daytime, using computer regulated systems. Increased UV-B radiation had little effect on growth expressed as fresh weigh and leaf area. Water soluble low molecular weight compounds showing absorption in near UV region were enhanced according to the irradiated UV-B dose. One of compounds in cauliflower leaves was identified as chlorogenic acid. This was found to have no near-UV photosenSitizerable activity and is known to have an ability to scavenge a wide species of active oxygen. Another pro-oxidant compound that generates superoxide anion radical under near-UV irradiation was not induced by increased UV-B during cultivation, and identified as lumazine, a degradation product from folic acid.

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A Framework for OAM in OBS Networks (OBS 네트워크의 OAM 기본 체제)

  • Shin Jong-Dug
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.1B
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    • pp.41-51
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    • 2004
  • Operation and maintenance (OAM) in optical burst switching (OBS) networks has not yet been addressed even though OBS has been gaining research interest in recent years. In this paper, we defined five OBS functions such as Burst Termination (BT), Burst Transmission (BTX), Burst Switching (BSW), Routing and Switching Control (RSC), and Protection and Restoration (PAR). A functional model for OBS networks and an OAM architecture are designed to meet the operational requirements. We present the first framework to realize OAM in OBS networks, including OAM activities, OAM-capable OBS nodes such as ingress edge, core, and egress edge nodes, OAM information and communication models and protocols. A number of examples of possible network failures are pointed out and the corresponding reactions to these using the proposed OAM architecture are presented.

Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

  • OCK Mee Sun;KIM Bong Jin;KIM Sun Mi;BYUN Kang Hyun
    • Parasites, Hosts and Diseases
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    • v.43 no.3 s.135
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    • pp.101-110
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    • 2005
  • In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited $42-57\%$ homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GOSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

An Optimal Power Scheduling Method Applied in Home Energy Management System Based on Demand Response

  • Zhao, Zhuang;Lee, Won Cheol;Shin, Yoan;Song, Kyung-Bin
    • ETRI Journal
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    • v.35 no.4
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    • pp.677-686
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    • 2013
  • In this paper, we first introduce a general architecture of an energy management system in a home area network based on a smart grid. Then, we propose an efficient scheduling method for home power usage. The home gateway (HG) receives the demand response (DR) information indicating the real-time electricity price, which is transferred to an energy management controller (EMC). Referring to the DR, the EMC achieves an optimal power scheduling scheme, which is delivered to each electric appliance by the HG. Accordingly, all appliances in the home operate automatically in the most cost-effective way possible. In our research, to avoid the high peak-to-average ratio (PAR) of power, we combine the real-time pricing model with the inclining block rate model. By adopting this combined pricing model, our proposed power scheduling method effectively reduces both the electricity cost and the PAR, ultimately strengthening the stability of the entire electricity system.