• Parasites, Hosts and Diseases
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• v.49 no.3
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• pp.235-243
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• 2011

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.

• Journal of Families and Better Life
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• v.15 no.2
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• pp.141-154
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• 1997

The purpose of this study was to investigate the influence of perceived adequacy resource(PAR) and locus of control on the stress from the family resources management in housewives. The subjects of this study were 474 married women who lived in apartment in Taegu, . A questionaire was used as survey method. Factor analysis and MANOVA were employed for data analysis and Scheffe test for post-hoc analysis, . The main findings were ; First the effect of PAR on the degree of the stress from the family resources management was not different according to locus of control. Second the stress from the family resource management was found to be significantly different according to PAR.

• Journal of Satellite, Information and Communications
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• v.10 no.2
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• pp.60-69
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• 2015

A new and effective Doppler effects compensation method based on constellation estimation as well as an average power saving strategy by modified modulation scheme utilizing zero point constellation are presented with theoretical analysis and relevant simulations. The suggested schemes are proved to be very effective for satellite OFDM signals relayed by power limiting transponder for the mobile terminal on high speed vehicles.

• Journal of IKEEE
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• v.2 no.2 s.3
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• pp.247-254
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• 1998

This paper presents a performance study of two LU decomposition algorithms on two massively parallel SIMD machines: the 16K processor MasPar MP-1 and the 4K processor MasPar MP-2. The paper presents experimental results and an analysis of the algorithms to explain the results. While the blocked and the nonblocked algorithms for LU decomposition have been studied individually by others, we compare the two algorithms and identify the tradeoffs between them. Our analysis of the blocked algorithm shows how the block size affects the interprocessor communication cost and the memory read/write overhead. The analysis in this paper is useful to determine an optimum block size for the blocked algorithm.

• Proceedings of the Korean Institute of Communication Sciences Conference
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• 2006.07a
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• pp.551-551
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• 2006

• Korean Journal of Veterinary Service
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• v.42 no.3
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• pp.153-159
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• 2019

The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6')-lb-cr and qepA in 40 nalidixic acid- resistant ($NA^R$) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 $NA^R$ Salmonella isolates was $128{\sim}512{\mu}g/mL$ and < $0.125{\sim}0.25{\mu}g/mL$, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All $NA^R$ Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: $Asp87{\rightarrow}glycine$, $Ser83{\rightarrow}tyrosine$. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

• Journal of radiological science and technology
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• v.24 no.2
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• pp.35-40
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• 2001

Performance of SPECT imaging systems which use a rotating gamma camera, are affected by characteristics of the detector-collimator assembly, the data acquisition method, and the filter used in imaging reconstruction. The purpose of this study Is to examine image qualifies of SPECT with different types of low energy collimators. The SPECT imaging system in this study is a digital gamma camera system GCA-901A(Toshiba) and a data processing unit Scintipac-700(Shimadzu). The four types of collimators compared are UHR(ultra high resolution), LEHR(low energy high resolution), LEGP(low energy general purpose), and I-123 PAR(Parallel), with 0.27, 0.66, 1.00, and 2.06 relative sensitivity, respectively. In this case of the same collimators, the spatial resolutions measured in the slice plane showed a slight difference in the FWHM values(mean values of UHR, LEHR, LEGP, and I-123 PAR were 11.3 mm, 13.6 mm, 15.8 mm, and 20.4 mm, respectively) between the center and the circumference of the field of view, in the radial direction, but a large difference in the tangential direction, with lower FWHM values(values of UHR, LEHR, LEGP, and I-123 PAR were 8.4 mm, 8.7 mm, 9.3 mm, and 10.8 mm at 12 cm from the center, respectively). In comparison of SPECT images with the four types of collimators, except for the I-123 PAR collimator, image qualities of UHR, LEHR, and LEGP collimators showed only a slight difference. From the point of for, it is expected that the LEGP collimator would be suitable for SPECT imaging with $^{99m}Tc$.

• The Korean Journal of Ecology
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• v.27 no.6 s.122
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• pp.363-367
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• 2004

Photosynthetically active radiation (PAR) beneath the forest canopy, understory species richness and diversity, and biomass were measured in a Larix leptolepis plantation seven years after thinning in Yangpyeong. Four different thinning intensities (control, $10\%,\;20\%\;and\;40\%$ stocking reduction) were applied in 1997. The current PAR values were lower than those measured four years after thinning, and PAR at the heavy thinning plots was significantly higher than that of other thinning intensities. A total of 23 species including 9 tall-trees and 14 shrubs were found for the high layer while a total of 82 species including 10 tall-trees, 29 shrubs, and 43 herbs for the low layer. Species richness and diversity generally increased with thinning intensities, and the trends were more evident for the low layer. Aboveground biomass significantly differed among thinning intensities for both shrubs and herbs. Also there was a negative correlation between biomass and the current number of stems per hectare. The current study suggested that the effects of thinning on light conditions at the forest floor, species richness and diversity and production of understory vegetation continued seven years after the treatment.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.222-228
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• 2001

Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

• YAKHAK HOEJI
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• v.41 no.2
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• pp.247-254
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• 1997

The proteinase-activated receptor (PAR-2) belongs to the family of seven transmembrane region receptors, like the thrombin receptor, it is activated by specific proteolytic clea vage of its extracellular amino terminus and a synthetic peptide (SLIGRL). The earthworm protein fraction (EPF) extracted from Lumbricus rubellus elicted dose- and endothelium-dependent relaxations in phenylephrine-contracted rat thoracic aorta, whereas heat inactivated EPF (0.5 ${\mu}g$ /ml) had no effect. In the presence of the nitric oxide synthase inhibitor NG-methyl-L-arginine (1.8 micro M), EPF (0.5 ${\mu}g$ /ml)-induced relaxations were partially inhibited. Furthermore, EPF (0.5 ${\mu}g$ /ml) dramatically caused relaxation of thrombin-desenstized rat thoracic aorta. These results indicate that EPF activates PAR-2 in vascular endothelial cell. Intravenous injection of EPF (20 mg/kg, bolus) into anesthetized rats produced a marked depressor response. EPF (0 ~ 80 ${\mu}g$ /ml, gradient) was very effective on increasing of perfusion volume in rabbit ear vessel preparations. These results imply the usefulness of EPF as a vascular smooth muscle relaxant and indicate that the activation of PAR-2 may be a mechanism of EPF on hemokinetic improvement.