• Title/Summary/Keyword: Panax ginseng root

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Production of Saponin by Hairy Root Cultures of Ginseng (Panax ginseng C.A. Meyer) Transformed with Agrobacterium rhizogenes (Agrobacterium rhizogenes에 의하여 형질전환된 인삼(Panax ginseng C.A. Meyer)의 모상근 배양에 의한 Saponin 생산)

  • Hwang, Baik;Ko, Kyeong-Min;Hwang, Kyeong-Hwa;Hwang, Sung-Jin;Kang, Young-Hee
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.289-296
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    • 1991
  • Cultures of hairy root induced from ginseng(Panax C.A. Meyer) transformed with Agrobacterium rhizogenes (strain A4, ATCC 15834) were established and morphologically two different hairy root strains (HB1, HB2) were obtained. To determine the optimum growth rate, the hairy root (HB2) was cultured in various liquid medium supplemented with or without plant growth hormone. The growth rate of hairy root cultured on MS medium was 1.3-3.1 times higher than those cultured on other media, and the optimum sucrose concentration and pH were 3-6%, 5.5-6.5, respectively. Also, the growth rate of hairy root was increased when 0.02 M ammonium nitrate, 1.2 mM potassium phosphate (monobasic) and 0.5 mg/l IBA were supplied to liquid medium. The saponin patterns and contents of hairy root (HB2) were determined by TLC and HPLC. The crude saponin contents were 4.67% and the total saponin contents were 1.0%, on dry weight basis.

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Comparison of Grade of Raw and Red Ginseng on each Factor of Quality in Korean and American Ginseng (고려인삼과 미국삼의 품질요인별 수삼 및 홍삼등급 비교)

  • Chung, Chan-Moon;Shin, Ju-Sik
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.229-233
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    • 2006
  • Comparison of the grade of raw ginseng and that of red ginseng was investigated. The materials used in this study were Korean ginseng (Panax ginseng C. A. Meyer) and American ginseng(Panax quinquefolium L.) Coefficient of body term, length of main stem and weight of raw ginseng were used as the classifying criteria of the root size and grades. Korean ginseng distinguished the distribution of weight size from that of American ginseng. Korean ginseng distributed largely in middle and large root size, and American ginseng distributed largely in middle and small root size. American ginseng had shorter length of main root, bigger diameter of main root and more number of adventitious roots than Korean ginseng. The quality of Korean ginseng was better than that of American ginseng. In Korean ginseng, high quality of red ginseng above second grade (Jisam) was obtained, but low quality of red ginseng under third grade (Yangsam) in American ginseng. In Korean raw ginseng, the coefficients of body form of middle weight and large weight size were under 0.5, but those of American ginseng was over 0.5. So American ginseng were not adequate to produce good red ginseng. Those factors as length of main root and weight of main root were not significantly influenced on the qualify of red ginseng in both Korean ginseng and American ginseng. Coefficient of body form was leading factor affecting the quality of red ginseng. To improve the quality of red ginseng, coefficient of body form, weight of main root and length of main root were controlled adequately in both Korean ginseng and American ginseng.

Taxonomy of fungal complex causing red-skin root of Panax ginseng in China

  • Lu, Xiao H.;Zhang, Xi M.;Jiao, Xiao L.;Hao, Jianjun J.;Zhang, Xue S.;Luo, Yi;Gao, Wei W.
    • Journal of Ginseng Research
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    • v.44 no.3
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    • pp.506-518
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    • 2020
  • Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits the production of ginseng in China. The disease has long been thought to be a noninfectious physiological disease, except one report that proved it was an infectious disease. However, the causal agents have not been successfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in Northeast China. Fungi were isolated from the lesion and identified based on morphological characters along with multilocus sequence analyses on internal transcription spacer, β-tubulin (tub2), histone H3 (his3), and translation elongation factor 1α (tef-1α). Pathogens were confirmed by inoculating the isolates in ginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classified into 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani, F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel species I. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had the highest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated were pathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

Effect of Seeding Depth and of Soil Texture on Seeding Emergence and Root Shape of American Ginseng

  • Li, Thomas S.C.
    • Journal of Ginseng Research
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    • v.21 no.2
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    • pp.115-118
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    • 1997
  • Stratified American ginseng(Panax quinquefoilium L.) seeds were planted in a shaded greenhouse at four depths and in four different soil types to observe effects on emergence rate and root size. Seeding depth affected seedling emergence rates and the number of days required to complete emergence. The shape of the roots was affected by the texture of soil, especially percentage of sand.

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Effects of Vesicular-Arbuscular Mycorrhizae on The Growth of American Ginseng (V. A. Mycorrhizae가 미국삼의 생육에 미치는 영향)

  • Thomas, S.C.Li
    • Journal of Ginseng Research
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    • v.19 no.1
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    • pp.73-76
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    • 1995
  • Newly sprouted American ginseng (Panax quinquefolium L.) seedlings were transplanted to forest pots with mycorrhizae-infested soil and grown in screenhouse for 2 years. Growth patterns, mortality rate and fresh root weight were investigated. Plants in VAM soil had lower mortality rales than control. In soils infested with two species of mycorrhizal fungi (Glomu deseyicola, frappe, Bloss and Merge and G. intraradices, Schenck and Smith), 28-35% of plants produced 3-prongs in the second season and significantly increased fresh root weight by 41 to 43%.

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Effect of Soil Fumigation on Growth, Root Rot, and Red Discoloration of Panax ginseng in Replanted Soils (연작지 토양에서 토양연증제 처리가 인삼의 근부, 적변 및 생육에 미치는 영향)

  • 안용준;김홍진
    • Journal of Ginseng Research
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    • v.6 no.1
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    • pp.46-55
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    • 1982
  • The effect of soil fumigation on the growth, yield, root-rot and red discoloration of 2 year-old ginseng, Panax ginseng C. A. Meyer was investigated in the ginseng replanted fie14 Six soil fumigants, Cylone, Basamid, D-D, Dowfume MC-2, Telone C-17, and Vapam were applied in March, 1980, and 2 year-old ginseng plants were transplanted in April, 1981, and sampled in August, 1981. Growth an yields in Cylone and Basamid treated plots in the replanted soil were better than those in control. Especially, growth and yields of ginseng in Cyclone treated Plots were comparable to these in untreated virgin soil. Control effect of soil fumigants used on root-rot was in the order of Cylone, Basamid, Telone C-17, and the lest of them showed neglect fur effect. Frequency of red discoloration was 8% in Cyclone treated plots, 9% in Basamid treated plots 83.3% in the untreated ginseng replanted soil, and 2.5% in the untreated virgin soil.

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Change of Neutral Ginsenoside Contents in Red and Fresh Ginseng (Panax ginseng C. A. Meyer) by Hydrolysis (가수분해 처리에 의한 홍삼과 인삼의 중성 Ginsenoside 함량 변화)

  • Han, Jin Soo;Lee, Gang Seon;Tak, Hyun Seong;Kim, Jung-Sun;Ra, Jeong Woo;Choi, Jae Eul
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.1
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    • pp.23-31
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    • 2014
  • This study was carried out to investigate change of ginsenoside contents in red and fresh ginseng according to root part and age by hydrolysis. Neutral total ginsenoside contents by hydrolysis in 6-year main root and lateral root were significantly increased than those by non-hydrolysis, as 41.6 and 32.8%, respectively. However, there was no significant difference in red ginseng. In fresh ginseng, ginsenoside contents of the protopanaxatriol group such as Re, Rf, $Rg_1$, $Rg_2$, and $Rh_1$ were not significantly different, but $Rb_1$, $Rb_2$, $Rb_3$, Rc, and Rd showed significant difference. The increase rate of neutral total ginsenoside content by hydrolysis was higher in epidermis-cortex than stele. Also, the neutral total ginsenoside content was fine root > rhizome > lateral root > main root, respectively. While there was no tendency towards the increase of ginsenoside by hydrolysis with the increase of root age in fine root and rhizome, there was significant decrease in main root and lateral root.

Cytokinin signaling promotes root secondary growth and bud formation in Panax ginseng

  • Kyoung Rok Geem;Yookyung Lim;Jeongeui Hong;Wonsil Bae;Jinsu Lee;Soeun Han;Jinsu Gil;Hyunwoo Cho;Hojin Ryu
    • Journal of Ginseng Research
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    • v.48 no.2
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    • pp.220-228
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    • 2024
  • Background: Panax ginseng, one of the valuable perennial medicinal plants, stores numerous pharmacological substrates in its storage roots. Given its perennial growth habit, organ regeneration occurs each year, and cambium stem cell activity is necessary for secondary growth and storage root formation. Cytokinin (CK) is a phytohormone involved in the maintenance of meristematic cells for the development of storage organs; however, its physiological role in storage-root secondary growth remains unknown. Methods: Exogenous CK was repeatedly applied to P. ginseng, and morphological and histological changes were observed. RNA-seq analysis was used to elucidate the transcriptional network of CK that regulates P. ginseng growth and development. The HISTIDINE KINASE 3 (PgHK3) and RESPONSE REGULATOR 2 (PgRR2) genes were cloned in P. ginseng and functionally analyzed in Arabidopsis as a two-component system involved in CK signaling. Results: Phenotypic and histological analyses showed that CK increased cambium activity and dormant axillary bud formation in P. ginseng, thus promoting storage-root secondary growth and bud formation. The evolutionarily conserved two-component signaling pathways in P. ginseng were sufficient to restore CK signaling in the Arabidopsis ahk2/3 double mutant and rescue its growth defects. Finally, RNA-seq analysis of CK-treated P. ginseng roots revealed that plant-type cell wall biogenesis-related genes are tightly connected with mitotic cell division, cytokinesis, and auxin signaling to regulate CK-mediated P. ginseng development. Conclusion: Overall, we identified the CK signaling-related two-component systems and their physiological role in P. ginseng. This scientific information has the potential to significantly improve the field-cultivation and biotechnology-based breeding of ginseng.

The Change of Ginsenoside Composition in Notoginseng Root(Panax notoginseng) Extract by the Microwave and Vinegar Process (초단파 및 식초 처리에 의한 삼칠삼 추출물의 인삼 사포닌 성분 변화)

  • Jo, Hee Kyung;Cho, Soon Hyun;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
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    • v.45 no.4
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    • pp.320-325
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    • 2014
  • The purpose of this study is to develop a new preparation process of Notoginseng root(Panax notoginseng) extracts having high concentrations of ginsenoside $Rg_3$, $Rg_5$, $Rk_1$ and $Rh_4$, a special component of Red and Black ginseng(Panax ginseng). Chemical transformation from ginseng saponin to prosapogenin was analyzed by the HPLC. Extracts of Notoginseng root was processed under several treatment conditions including microwave and vinegar(about 14% acidity) treatments. Results of those treatments showed that the quantity of ginsenoside $Rg_3$ increased by over 7.6% at 15 minutes of pH 2~4 vinegar and microwave treatments. The results of processing with MPN-15 indicate that the microwave and vinegar(about 14% acidity) processed Notoginseng root extracts that had gone through 15-minute treatments were found to contain the largest amount of ginsenoside $Rg_3$(7.639%), $Rg_5$(6.061%), $Rk_1$(1.516%) and $Rh_4$(1.599). It is thought that such results provide basic information in preparing Notoginseng root extracts with functionality enhanced.

Comparison on the Protective Effect of the Root of Panax ginseng and the Root bark of Acanthopanax senticosus Against Lipid Peroxidation (인삼(人蔘)과 가시오갈피의 지질과산화억제작용(脂質過酸化抑制作用)에 관한 비교(比較) 연구(硏究))

  • Han, Yong-Nam;Kwon, Yun-Kyung;Han, Byung-Hoon
    • Korean Journal of Pharmacognosy
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    • v.12 no.1
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    • pp.26-30
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    • 1981
  • The antioxidant activities of the root of Panax ginseng and the root bark of Acanthopanax senticosus were compared by in vitro and in vivo experiments. The results of in vitro experiments showed no significant difference between the antioxidant activities of both crude drugs. However, the results of in vivo experiments showed much higher antioxidant activities in Panax ginseng than Acanthopanax senticosus, showing about 80 times greater activity.

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