• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.289-295
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• 2013

This study was carried out to investigate the cause of leaf discoloration occurring frequently in paddy cultivation. Chemical property of soil and inorganic nutrient component of leaf were analyzed on abnormal fields of 7 regions where leaf discoloration occurred severely and normal fields of 7 regions among ginseng garden. The pH of abnormal fields was strong acidic condition (pH 5.51) compare to normal fields of slightly acid condition (pH 6.42). Calcium and magnesium content in abnormal fields were lower distinctly than that of normal fields, while EC, organic matter, phosphate, and potassium content showed not distinct difference between abnormal and normal fields. Whereas calcium and magnesium content were distinctly high in normal fields, both of potassium and iron content of ginseng leaf were distinctly high in abnormal fields. In particular, iron content of abnormal fields was more 1.94 times in soil, and 3.03 times in leaf than that of normal fields. In soil chemical property, there were significant negative correlation between leaf discoloration ratio and soil pH, and there were also significant positive correlation between leaf discoloration ratio and iron content. In ginseng leaf, there were highly significant negative correlation between leaf discoloration ratio and calcium content, and there were also highly significant positive correlation between leaf discoloration ratio and iron content.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.135-141
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• 1990

In order to elucidate the mechanism of the leaf-burning disease of ginseng (Panax ginseng C.A. Meyer), the relationships between thylakoid membrane peroxidation and chlorophyll bleaching were investigated in comparison with the ones of soybean (Glycine max L). When I measured the rate of lipid peroxidation in the thylakoids of ginseng and soybean by irradiation of light(60 w.m-2), it was identified that, the remarkably lower rate of lipid peroxidation was found in the ginseng thylakoid than the case of soybean. When lipid peroxidation of ginseng thylakoid was induced in the dark, chlorophyll contents of thylakoid was not changed. The results suggest that lipid peroxidation does not affect the chlorophyll bleaching in ginseng thylakoid. Thylakoid membrane peroxidation as well as chlorophyll bleaching was closely related with photosynthetic electron transport. But, according to the quenching experiment active oxygen species induced lipid peroxidation may be different species in the case of chlorophyll bleaching.

• Journal of Ginseng Research
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• v.15 no.1
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• pp.21-30
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• 1991

This study was conducted to know the elects of light intensity of polyethylene net shading on the growth status, photosynthesis and root yield of ginseng plants. Polyethylene net shading of loft transmittance was the best one among light intensities of polyethylene net used in view of photosynthesis and decreasing of early leaf defoliation. According to increase of light intensity under the shading chlorophyll contents of ginseng leaves were decreased. As it was increased over 2 mg/g Photosynthesis and total saponin of leaves showed on the decrease remarkably. The rate of alternaria blight of ginseng plants showed the positive correlation between light intensity and leaking rate. The shading of 10% transmittance in root yield was increased by 40% in 6-year-old ginseng plant as compared with common straw shading, due to decreased missing plant and increased root weight.

• Journal of Ginseng Research
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• v.18 no.3
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• pp.175-181
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• 1994

We studied the folilar wiping effects of antioxidants (ascorbate, glutathione and sodium azide), which effectively inhibited the chlorophyll bleaching or completely recorved the early stage of photosynthesis of Panax ginseng C.A. Meyer, on photosynthesis, stomatal resistance, free sugar, starch, and total saponin contents of ginseng under the excess light intensity (45 kLux) during 6 days. Ascorbate and glutathione, endogenous antioxidant, recovered photosynehtsis and stomatal resistance, and reduced the photoinhibition by the excess light intensity (45 kLux) on free sugar, starch and total saponin contents. But sodium azide, exogenous $^{1}O_2$ quencher, showed negative effect. Therefore, we assumed that carbohydrates and saponin metabolisms of ginseng by antioxidants (ascorbate, glutathione) were normal. For the reduction of inhibition by excess light in ginseng a program for the higher activation of antioxidants and antioxidative enzymes in ginseng leaf will be desirable. Key words Antioxidants, ascorbate, glutathione, Photoinhibition, ginseng.

• Journal of Ginseng Research
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• v.17 no.3
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• pp.240-245
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• 1993

This study was conducted with ginseng plants to investigate photosynthetic response to changes of light intensity and leaf temperature. $CO_2$ uptake in diurnal course was highest in the first phase (8 00~5 : 30 Am.) on May 30, 1992. In $CO_2$ uptake related to stomatal conductance, these relationship was synchronized in diurnal course, but relationship between TEX>$CO_2$ uptake and intercellular $CO_2$ concentration in diurnal course was synchronized oppositely. Leaf temperature and light intensity at the highest $CO_2$ uptake were in the range of 23~$24^{\circ}C$) and 95$\mu$mol.$m^{-2}$.$s^{-1}$), $CO_2$ , respectively. In response to an increasing light intensity under a constant leaf temperature ($18^{\circ}C$), $CO_2$ uptake was increased throughout the light intensity sequence up to 250$\mu$mol.$m^{-2}$.$s^{-1}$), $CO_2$ When $CO_2$ uptake was measured with a series of leaf temperature under a constant light intensity (250 $\mu$mol.$m^{-2}$.$s^{-1}$), $CO_2$ uptake was highest at $18^{\circ}C$ as a 4.1$\mu$mol.$m^{-2}$.$s^{-1}$), $CO_2$ . Similar changes were also observed in stomatal conductance and intercellular $CO_2$ concentration. Evidences from several approaches indicate that synchronization of $CO_2$ uptake, stomatal conductance and intercellular $CO_2$ concentration were closely inter-related and changes of leaf temperature iuluenced the photo-response in photosynthetic processes.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.45-48
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• 2006

The characteristics of plantlets redifferentiated from calli of F1 hybrid between Panax ginseng and Panax quinquefolius were investigated. Growth of plantlets redifferentiated from F1 hybrid was superior to the plants redifferentiated from Korean ginseng. Stem color of plantlets redifferentiated from F1 hybrid was more purple than that from Korean ginseng and leaf color of the former was also greener than that of the latter. Chunpoong, Yunpoong and Seonweon which are belonged to Korean ginseng showed same PCR band(A), while American ginseng showed different PCR band (B) in Internal Transcribed Spacer (ITS) region. F1 hybrid exhibited both A and B PCR band which belonged to Korean ginseng and American ginseng, respectively. F1 hybrid calli and plantlets redifferentiated from F1 hybrid calli showed same PCR band with that of F1 hybrid plant in ITS region. Therefore it was confirmed that piantlets redifferentiated from F1 hybrid exhibited genetic stability in ITS region.

• Journal of Ginseng Research
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• v.8 no.2
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• pp.178-183
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• 1984

This study was conducted to know the influence of plant age and cultural conditions such as plant population density and light intensity under the shading on the leaf shape of panax ginseng. The result obtained were as follows; 1) Leaf length(L)/maximum width(W) was no difference with different age of over 3-over 3year old plant, but that of 1 or 2-yearold was smaller than those of over 3-year old. The values of L/W showed in the order of 2 or 4,3, 1 or 5 leaflet. 2) Ratios of leaflet area to leaf area were 32.0% in leaflet 3, from 209.% to 27.9% in leaflet 2 or 4, and from 6.5% to 7.1% in leaflet 1 or 5. 3) The coefficients of variability for L/W and ratio of leaflet area to leaf area of leaflet 3 were smallest among leaflets. 4) There were significant differences between largest and smallest leaflet 3, leaf areas and ratio of leaflet 3 area to leaf area in same plant. 5) LW and ratio of leaflet 3 area to leaf area were not affected by plant population density. 6) It showed a tendency that the L/W was increased with increasing the light transmittance rate (LTR). The ratio of leaflet 3 area to leaf area of ginseng grown under 20% LTR was not different comparing to that of plant grown under 5% LTR, but it was significantly increased in plant grown at 100% LTR.

• Journal of Ginseng Research
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• v.38 no.1
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• pp.73-77
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• 2014

Field and nutrient cultures of American ginseng (Panax quinquefolius L.) were used to establish foliar symptoms related to boron (B) concentration in leaves and soils, and to evaluate radish as a time-saving model system for B nutrition. Application of excess B, 8 kg/ha versus the recommended 1.5 kg/ha, to field plantings of 2-, 3-, and 4-yr-old American ginseng plants just prior to crop emergence caused, within 4 wk after crop emergence, leaf symptoms of chlorosis followed by necrosis starting at the tips and progressing along the margins. The B concentration in leaves of 2-4-yr-old plants receiving 1.5 kg/ha Bwas $30{\mu}g/g$ dry mass compared to $460{\mu}g/g$ dry mass where 8 kg/ha B was applied. Similarly, B concentration in soils receiving the lower B concentration was 1.8 mg/g dry mass and $2.2-2.8{\mu}g/g$ dry mass where the higher B concentration was applied. Application of 8 kg/ha B reduced the dry yield of 3rd-yr roots by 20% from 2745 kg/ha to 2196 kg/ha and 4th-yr roots by 26% from 4130 kg/ha to 3071 kg/ha. Ginseng seedlings and radish were grown under greenhouse conditions in nutrient culture with four B concentrations ranging from 0 mg/L to 10 mg/L. At 5 mg/L and 10 mg/L ginseng and radish developed typical leaf B toxicity symptoms similar to those described above for field-grown plants. Increasing B in the nutrient solution from 0.5 mg/L to 10 mg/L decreased, in a linear fashion, the root and leaf dry mass of ginseng, but not radish. Given the many similarities of ginseng and radish to B utilization, radish might be used as a timesaving model system for the study of B, and other micronutrients, in the slow-growing perennial ginseng.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.158-164
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• 1989

In order to determine the relationships between the lea(-burning disease and the light harvesting chlorophyll-protein (LHCP) complex in Panax ginseng C. A. Meyer, we investigated the chlorophyll-protein (CP) complex of the thylakoid membrane and its characteristics. In P. ginseng four Cp-complex bands determined by non-denaturing SDS-PAGE were identified CP I'(containing reaction center of photosystem I and LHCP I antennae), CP I (reaction center of photosystem I) LHCP II** (oligoform of LHCP II), and LHCP II (photosystem II antennae, CP 26 and CP 29) by Bassis and Dunahay's procedures. Under our experimental condition, the CP I band was only observed in P. ginseng and the band intensity of LHCP II** in P ginseng was higher than in spinach and soybean. There were differences in the absorption and fluorescence spectra and chlorophyll a/b ratio of the CP-complex bands between P. ginseng and other Plants. The Polypeptidr content of P. ginseng thylakoid was lower than in spinach and soybean thylakoid, and the Polypeptide profiles of P. ginseng was low band intensity, especially about 29-35 kD, 55 kD, and 60 kD, compared to spinach and soybean. The inhibitory effects of 2,5-dimethylfuran, specific singlet oxygen ($^1O_2$) quencher, showed that singlet oxygen destroyed 60% of chl.a, 90% of chl.b and 70% of carotenoid in bleaching P. ginseng with leaf-burning disease.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.2
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• pp.157-162
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• 1987

The study was carried out to investigate the effects of light intensity, temperature and seasonal trends on the photosynthesis as well as the physiological characteristics of Panax species and cultivars. Four-year-old plant of Violet-stem variant, Yellow-berry variant, Mimaki and Russian ginseng of Panax ginseng C. A. Meyer and American ginseng of Panax quiquefolium L. were used for study. These Panax species and cultivars were cultivated under the straw shading. The light saturation point of leaves in Violet-stem variant, Yellow-berry variant, Mimaki and American ginseng were 15,000 lux, but that of Russian ginseng was 10,000 lux. The optimum air temperature on the phtosynthesis of Violet-stem variant, Yellow-berry variant, Mimaki and American ginseng were 20$^{\circ}C$ but that of Russian ginseng was 15$^{\circ}C$ under 15,000 lux. The photosynthetic rates were increased in order of Russian ginseng, Mimaki, Yellow-berry variant, Violet-stem variant and American ginseng. The respiration rates of ginseng leaves were increased according to the increasing of temperature, but it was not different among Panax species and cultivars. Stomata frequency of American ginseng was highest, that of Russian ginseng lowest, while the length of stomata was reverse. The total chlorophyll content of American ginseng and specific leaf weight of Mimaki were higher than other ginseng cultivars. The root weight of American ginseng was heavier than others, but that of Russian ginseng was lighter. The num ber of leaflets per plant of 2-year-old plant and the root weight of 6-year-old plant were increased in order of Russian ginseng, American ginseng, Mimaki, Yellow-berry variant and Violet-stem variant.