• Journal of Ginseng Research
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• 제18권1호
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• pp.44-48
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• 1994

In adrenaline-stimulated human platelets, panaxadiol (PD) and panaxatriol (PT) from Panax ginseng C.A. Meyer did not inhibit the $Ca^{2+}$-innux, but inhibited the formation of thromboxane $A_2$ and the platelet aggregations. It seems that PD and PT block a pathyway interconvefing arachidonic acids (20:4) to thromboxane $A_2$ (TX $A_2$), because the amount of $Ca^{2+}$ which phospholipase C or phospholipase $A_2$ requires to liberate 20 : 4 from membrane phospholipids was increased by PD and PT. These results mean that PH and PT have an antiplatelet effect by Inhibiting the formation of TX $A_2$.

• Journal of Ginseng Research
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• 제17권3호
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• pp.232-235
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• 1993

We investigated the effect of antioxidants (ascorbate, glutathione, and sodium azide), which efEectively inhibited the chlorophyll bleaching of Panax ginseng CA Meyer under the high light intensity, treated by folilar wiping on the early stage of photosynthesis and transpiration of ginseng in the 5000 $\mu$mol photon.$m^{-2}$.$s^{-1}$. Ascorbate and glutathione, endogenous antioxidant, completely recovered ginseng from the photoinhibition, but sodium azide, synthetic quencher, showed negative effect. We assumed that endogenous antioxidants could be available to the protection of the leaf-burning phenomenon of ginseng.

• 한국식품과학회지
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• 제12권1호
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• pp.1-5
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• 1980

고려 인삼(Panax ginseng C. A. Meyer)중의 invertase를 연구하기 위하여 조(租) 인삼 invertase를 분리 조제하여 그 성질을 조사해 본 결과 다음과 같았다. 1. 조 인삼 invertase는 ammonium sulfate $0.8{\sim}1.0$포화 분획에 의하여 효과적으로 조제되었다. 2. 조 인삼 invertase는 pH $5{\sim}9\;35^{\circ}C$이하의 조건에서 안정성을 나타내었다. 3. 조 인삼 invertase는 최적 pH 5.0, 최적온도 $50^{\circ}C$를 나타내었다. 4. 조 인삼 invertase는 $Ag^{+}\;Mn^{+}\;Hg^{+}\;Zn^{+}\;Rb^{+}$등의 금속 이온에 의하여 저해되었으나 그 저해 정도는 크지 못하였다. 한편 $Ca^{+}\;Cu^{+}\;Fe^{3+}$등에 의한 효소 활성 증대 효과는 특이적이었다.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1379-1382
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• 2008

This study was performed to provide basic information that can be used to differentiate Korean ginseng (Panax ginseng CA. Meyer) berry and seed from American ginseng (Panax quinquefolium L.) seed. Total ginsenoside contents of Korean ginseng berry, Korean ginseng seed, and American ginseng seed were 9.09, 3.30, and 4.06%, respectively. Total ginsenoside content of Korean ginseng berry was about 2.2 to 2.7 times higher than those of Korean ginseng seed and American ginseng seed. Particularly ginsenoside Re content of 4-year cultivated Korean ginseng berry (5.99%) was about 3.6 to 5.4 times higher than that of 4-year cultivated Korean ginseng seed (1.65%) and 4-year cultivated American ginseng seed (1.10%). The contents of total ginsenoside and ginsenoside Re of Korean ginseng berry were about 4.8 and 28 times higher, respectively, than those of 4-year cultivated Korean ginseng root. In general the contents of total ginsenoside and ginsenoside Re of Korean ginseng berry were significantly higher than those of Korean ginseng seed and American ginseng seed.

• Journal of Ginseng Research
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• 제4권1호
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• pp.55-64
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• 1980

The distribution pattern of mineral nutrients, among various Parts of Korea ginseng (Panax ginseng C.A. Meyer) was investigated to understand ginseng nutrition by simple correlation analysis. Five·year old ginseng plants grown under four different nutritional environments were sampled and separated into leaf, petiole, stem, rhizome, cortex and epidermis of tap foot, central part of tap root, cortex and epidermis of lateral root, central part of lateral root, fine root in the middle of truly, for chemical analysis. Between mineral nutrients in root, N and P showed highly significant positive correlation each other and with Mg and Cu while all other elements (K, Ca, Mg, Fe, Mn, Zn, Cu, B) showed highly significant positive correlation each other. In shoot, number of mineral nutrient pairs haying significant relation was much less than in root. (Negative: P with Ca or B, K with N, Fe, Mn or Cu, Positive: N with Mg, Fe, Mn or Cu, K with Zn, Ca with Mg, Zn, or B, Fe Mn Cu each other, Mn with B.) The number of pairs having significant correlation in whole plant was approximately the same as the number in root but three of them showed significant negative correlation. The distribution pattern similarity of mineral contents among various parts was also discussed in relation to physiological significance in Korea ginseng plant.

• Journal of Ginseng Research
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• 제19권2호
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• pp.160-164
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• 1995

This study was carried out to compare the root yields, root characters, saponin and ginsenosides contents of 6-year-old p. ginseng and p. quinquefolium. These two ginseng species showed difference in the diameter and ten비h of mainroot. The main root length or p. quinquefolium was shorter than that of p. ginseng, whereas Jakyung-jong and Hwangsook-jong of P. ginseng showed similar root length. Proximate composition were similar between the two species, however, crude fibercontent was significantly higher in main and lateral root of Jakyung-jong and Hwang sook-jong of P ginseng than P quinquefolium. In regard to mineral contents of root, P ginseng contained more Ca and Mn and less Fe and Al than P. quinquefolium. P. quinquefolium contained more of Rbl and Rd of protopanaxadiol saponin, and less or Re, $Rg_1$ and $Rg_2$ of protopanaxatriol saponin than P ginseng. However, no Rf was detected in the p. quinquefolium. Key words Panax ginseng, Panax quinquefolium, ginseng character, ginsenoside.

• Journal of Ginseng Research
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• 제33권1호
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• pp.59-64
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• 2009

$Ca^{2+}$ and calmodulin (CaM), a key $Ca^{2+}$ sensor in all eukaryotes, have been implicated for defense responses of plants. Eukaryotic CaM contains four structurally and functionally similar $Ca^{2+}$ domains named I, II, III and IV. Each $Ca^{2+}$ binding loop consists of 12 amino acid residues with ligands arranged spatially to satisfy the octahedral symmetry of $Ca^{2+}$ binding. To investigate the altered gene expression and the role of CaM in ginseng plant defense system, cDNA clone containing a CaM gene, designated PgCaM was isolated and sequenced from Panax ginseng. PgCaM, which has open reading frame of 450 nucleotides predicted to encode a precursor protein of 150 amino acid residues. Its sequence shows high homologies with a number of other CaMs, with more similarity to CaM of Daucus carota (AAQ63461). The expression of PgCaM in different P. ginseng organs was analyzed using real time PCR. The results showed that PgCaM expressed at different levels in young leaves, shoots, and roots of 3-week-old P. ginseng. In addition, the expressions of PgCaM under different abiotic stresses were analyzed at different time intervals.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.79-85
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• 1990

The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and comitogenic activities in human peripheral blood Iymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose. DEAE-Sepharose) yielded a semi-purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in Iymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.

• 한국식품과학회지
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• 제17권4호
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• pp.240-244
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• 1985

고려인삼(Panax ginseng C. A.. Meyer) 중의 $\beta$-amylase를 연구하기 위하여 조(粗)인삼 $\beta$-amylase 를 분리한 후 그의 성질을 조사하였다. 조인삼 $\beta$-amylase는 ammonium sulfate 0.2$\sim$0.6포화분획에 의하여 효과적으로 조제되었다. 조제된 본 효소는 전형적인 $\beta$-amylase의 작용을 하여 starch에서 maltose만을 생산하였으며 maltase의 활성은 나타내지 않았다. 본 효소는 pH5$\sim$9(특히 pH7$\sim$8), $40^{\circ}C$ 이하의 조건하에서 안정성을 나타내었고 최적pH 5.0, 최적온도 $35^{\circ}C$를 나타내었다. 본 호소는 기질(starch)농도 l2mg% 이하에서 기질농도에 비례하여 호소활성이 증가하였으며 Km치는 4.76mg%이었다. 또 본 효소는 $K^{+}$, $Na^{+}$, $An^{++}$,$Ca^{++}$, $Co^{++}$,$Mn^{++}$ $Zn^{++}$에는 영향을 받지 않았으나$Ag^{+}$, $Hg^{++}$,$Cd^{++}$, $Cu^{++}$,$ Al^{3+}$, and $Fe^{3+}$ 등에는 현저한 저해를 받았다.

• Journal of Ginseng Research
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• 제18권2호
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• pp.95-101
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• 1994

Saponin of Panax ginseng (C.A. Meyer) is composed of Protopanaxatriol (PT) and Protopanaxa- diol (PD). We investigated the effects of PT and PD on the contractility and $^{45}Ca$ uptake in the pig coronary artery. Isometric tension in the helical strips and $^{45}Ca$ uptake in the ring strips were measured in the presence or absence of PT and PD. PT and PD did not affect the high K+ (40 mM)-induced contraction but relaxed the ACh-induced contraction in a dose4ependent manner (1~10 mg/dl). The vasorelaxing effect of PT on the ACh-induced contraction was more potent than that of PD. Those relaxations were partially suppressed by the rubbing of endothelium removal. ACh-induced contraction in the $Ca^{2+}$-free Tyrode's solution was suppressed by the pretreatment of PT or PD. Following the depletion of ACh-sensitive intracellular $Ca^{2+}$ pool, ACh-induced contraction was suppressed by the pratreatment of PT or PD. With the pretreatment of PT or PD, $^{45}Ca$ uptake by high K+ (43 mM) was not changed but that by ACh was suppressed in the pig coronary artery. From the above results, we suggested that the vasorelaxing effect of PT and PD of Panax ginseng was due to inhibition of intracellular $Ca^{2+}$ release, inhibition of $Ca^{2+}$ uptake via receptor-operated $Ca^{2+}$ channels and in part a release of vasorelaxing factor from endothelium in pig coronary artery.