• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.269-272
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• 2004

After concrete casts, temperature decent and shrinkage bring volume changes of concrete pavement. Microcracking and cracking in concrete pavement are caused by these volume changes. As a result, durability of concrete pavement is deteriorated. Recently, Very-Early Strength Latex Modified concrete(below:VESLMC) from the beginning of High-Way is used as urgent repair material for bridge deck. The advantage of VESLMC is that compressive and flexural strength at 3 hours age are 4.5MPa and 21MPa respectively. It allows the traffic to open in 3 hours. But, this material has the problem which is early-age shrinkage cracking caused by water self-dissipation with rapid hydration reaction and water evaporation with body dry. Unfortunately, until now, the research about early-age shrinkage of VESLMC leaves something to be desired. Therefore, the purpose of this study is to present the early-age shrinkage of VESLMC respect to latex contents and shrinkage ratio to maximum length change that can help field engineers' skill. Latex contents of 0, 5, 10, 15, $20\%$ in standard of same workability in VESLMC are selected by experimental variables. After initial set, shrinkage value was measured with 10mm LVDT for 3 days. The results of maximum shrinkage ratio were 0.019, 0.017, 0.023, $0.027\%$ respectively.

• Clinical and Experimental Pediatrics
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• v.45 no.4
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• pp.454-458
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• 2002

Purpose : Bacillus Calmette-$Gu\acute{e}rin$(BCG) lymphadenitis is one of the most common complications of BCG vaccination. The involved lymph nodes usually subside spontaneously, but they may become enlarged and form an abscess. Treatment of these infants is controversial. The Pan American Health Organization recommends local isoniazid or rifampicin instillation for patients with suppurative BCG lymphadenitis. Methods : The study group comprised 37 patients who presented with BCG lymphadenitis over the last three years. BCG lymphadenitis was diagnosed if the affected patient developed an ipsilateral axillary or supraclavicular lymphadenitis, with no other identifiable cause for the lymphadenitis. We used rifampicin instillation therapy for patients with suppurative BCG lymphadenitis. Results : Lymphadenitis regressed spontaneously in three patients. Thirty four patients showed a progression to abscess formation. Among 34 patients with suppurative lymphadenitis, drainage developed spontaneously during the follow-up period in nine patients before therapy. Twenty five patients received needle aspiration and local rifampicin instillation therapy. Reaspiration was performed in seven patients. One of these patients still has large lymph nodes after the second attempt. Conclusion : Needle aspiration and local rifampicin instillation therapy into the node is a safe and effective form of treatment for suppurative BCG lymphadenitis.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2006.06b
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• pp.289-299
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• 2006

This paper aims to provide strategies to attract container cargoes for the Incheon port by analysing O/D of the container cargoes with the year-2004 export and import data from. The Korean customs service. O/D analysis was carried out with establishing optimal zones, which are defined as City, Gun, Gu(Korean administrative districts) to which the export-import service can be provided from a certain port with minimized freight(or transport) cost and stevedoring fee. For the Incheon port, 35 administrative districts including Seoul and Incheon in Kyunggi-Do and Kangwon-Do are recognized as the optimal zones, and approximately 25.50% of around 2.02-million-TEU per year of the cargoes from the woes are exported and imported through the pan. The strategies to attract container cargoes was suggested by comparing the Incheon port to supposed-competitive pons. The Busan portn(64.89%), Guangyang port(4.46%) and Pyeongtaeck port(3.35%) are supposed as the competitive pons which have a large proportion of handling the cargoes from the optimal zones. When comparing the Incheon port to these ports, The Incheon pan requires a distinctive cost strategy, providing incentives to attract shipping companies and cargoes, improving efficiency related to loading-unloading at the port, and reinforcing Feeder-Network and advertisements about cost-saving to the shippers(the owners of goods) in the national capital region. Consequently, the mentioned requirements are suggested as the strategies to attract cargoes for the Incheon port.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.6
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• pp.641-647
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• 2015

Monitoring of soil fertility by long-term application of fertilizers is necessary to improve the fertility of soil and the productivity of crop. The objective of this study was conducted to investigate the changes of exchangeable Mg by continuous application of fertilizers from 1969 to 2014. The treatments were no fertilization (No fert.) and fertilization (NPK, NPK+C, NPK+S, and NPK+CS). The concentration of exchangeable Mg in No fert., NPK+C, and NPK+S treatments tended to increase from 1965 to 1975, but decrease gradually from 1976 to 1987, and increase again after 1988. Based on these, the changes of exchangeable Mg were divided into period I ('69 ~'75), period II ('76~'87), and period III ('88~'14). Especially, exchangeable Mg decreased in the period II. This was presumed that a significant amount of Mg from topsoil were leached into subsoil by break of plow pan and some of subsoil was incorporated into topsoil according to change of plowing depth by replacement of tillage machinery. It could be possible that exchangeable Mg in NPK, NPK+S, and NPK+CS was accumulated in the depth of 15~20 cm. For the period III, exchangeable Mg in No fert., NPK, NPK+C, NPK+S, and NPK+CS treatments increased at rates of 0.013, 0.018, 0.015, 0.023, and $0.024cmolckg^{-1}{\cdot}yr^{-1}$ respectively. Exchangeable Mg level in NPK+S was lower than the other treatments in the period I and period II, but higher than in the period III. This result was attributed to replacement of silicate fertilizer type from wollastonite (Mg 0.3%) to silicate fertilizer (Mg 3%). Also, exchangeable Mg level of No fert. treatment increased, which showed that Mg concentration of irrigated water had the greatest impact on Mg accumulation of soil. Recently, Mg level of irrigated water tended to increase, indicating that Mg concentration of water will affect greatly the concentration of exchangeable Mg of soil in the future. Like these, the changes of exchangeable Mg were greatly influenced by agricultural environment such as plowing depth, plow pan, content of fertilizer, and quality of irrigated water. Considering these agricultural environment, the proper management of soil is needed for the improvement of soil fertility and crop productivity.

• KSBB Journal
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• v.26 no.3
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• pp.199-205
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• 2011

Using tetrameric ${\beta}$-galactosidase as a model protein, anchoring motives were screened in Bacillus subtilis spore display system. Eleven spore coat proteins were selected considering their expression levels and the location in the spore coat layer. After chromosomal single-copy homologous integration in the amyE site of Bacillus subtilis chromosome, cotE and cotG were chosen as possible spore surface anchoring motives with their higher whole cell ${\beta}$-galactosidase activity. PAGE and Wester blot of extracted fraction of outer layer of purified spore, which express CotE-LacZ or CotG-LacZ fusion verified the existence of exact size of fusion protein and its location in outer coat layer of purified spore. ${\beta}$-galactosidase activity of spore with CotE-LacZ or CotG-LacZ fusion reached its highest value around 16~20 h of culture time in terms of whole cell and purified spore. After intensive spore purification with lysozyme treatment and renografin treatment, spore of BJH135, which expresses CotE-LacZ, retained only 1~2% of its whole cell ${\beta}$-galactosidase activity. Whereas spore of BJH136, which has cotG-lacZ cassette in the chromosome, retained 10~15% of its whole cell ${\beta}$-galactosidase activity, proving minor perturbation of CotG-LacZ, when incorporated in the spore coat layer of Bacillus subtilis compared to CotE-LacZ. Usage of Bacillus subtilis WB700, of which 7 proteases are knocked-out and thereby resulting in 99.7% decrease in protease activity of the host, did not prevent the proteolytic degradation of spore surface expressed CotG-LacZ fusion protein.

• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.712-717
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• 2010

Cytochrome P450 enzymes (P450s) are involved in the synthesis of a wide variety of valuable products and in the degradation of numerous toxic compounds. The P450 BM3 (CYP102A1) from Bacillus megaterium was the first P450 discovered to be fused to its redox partner, a mammalian-like diflavin reductase. Here, we report the development of a whole-cell biocatalyst using ice-nucleation protein (Inp) from Pseudomonas syringae to display a hemeand diflavin-containing oxidoreductase, P450 BM3 (a single, 119-kDa polypeptide with domains of both an oxygenase and a reductase) on the surface of Escherichia coli. The surface localization and functionality of the fusion protein containing P450 BM3 were verified by flow cytometry and measurement of enzymatic activities. The results of this study comprise the first report of microbial cell-surface display of a heme- and diflavin-containing enzyme. This system should allow us to select and develop oxidoreductases containing heme and/or flavins into practically useful whole-cell biocatalysts for extensive biotechnological applications, including selective synthesis of new chemicals and pharmaceuticals, bioconversion, bioremediation, live vaccine development, and biochip development.

• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1163-1178
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• 2010

Plasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replication-participating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.9
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• pp.1727-1736
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• 2006

In a fuzzy control system to vocess fuzzy data in high-speed for intelligent systems, one of the important problems is the improvement of the execution speed in the fuzzy inference and defuzzification stages. Especially, it is more important to have high-speed operations in the consequent Pan and defuzzification stage. Therefore, in this paper, to improve the speedup of the fuzzy controllers for intelligent systems, we propose novel integer fuzzy operation method without mulitplications and divisions by only integer addition to convert real values in the fuzzy membership functions in the consequent part to integer grid pixels $(400{\times}30)$ without [0, 1] real operations. Also we apply the proposed system to the truck backer-upper control system. As a result, this system shows a real-time very high speed fuzzy control as compared as the conventional methods. This system will be applied to the real-time high-speed intelligent systems such as robot arm control.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.4 s.34
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• pp.97-110
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• 1999

The new stick make-up product was studied by using a gel, which is a viscous complex formed with clay minerals, vitamins A and E and fluorinated liquid polymer with a 1500 molecular weight. The gel cannot be obtained with any random combination of clay minerals and the ingredients described above. It takes the sequential manufacturing method as follows to get this kind of gel. Firstly, clay minerals and liquid polymers have to be pre-mixed in order to saturate the liquid polymers with the clay minerals. Then the on-processed gel has to be finely crystallized. The clay minerals, which are the core elements for this gel, were used as a function of Binder & Buffer and liquid polymer was mixed together for the deterioration of the surface tension of each component and to form a functional film in the gel. This liquid polymer was combined with clay minerals because it is not miscible with most oils and solvents. Waxes have a function of keeping a solid status in the stick. We reduced the usage of waxes by putting clay minerals as buffer in the proportion of 0.5:1 with oil phase. Ceramide takes care of the skin when used regularly and maintains the skin's moisture. Vitamins A and E contribute to preventing skin aging by the activation of skin cells. We could get the stable viscous gel, which has about 80% oil phase using clay minerals and liquid polymer. The crystalline structures of gel were surface-chemically-analyzed using SEM and Image Analyzer and were thermodynamically analyzed using DSC. Surface tension test and softness were done by Rheometer. In the end, these characteristics were verified by consumer panel tests in Seoul, Daegeon and Pusan in Korea and Hokkaido, Osaka and Miyazaki in Japan with correlation to the climate.

• Journal of Gastric Cancer
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• v.20 no.1
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• pp.81-94
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• 2020

Purpose: Duodenal stump leakage (DSL) is a potentially fatal complication that can occur after gastrectomy, but its underlying risk factors are unclear. This study aimed to investigate the risk factors and management of DSL after laparoscopic radical gastrectomy for gastric cancer (GC). Materials and Methods: Relevant data were collected from several prospective databases to retrospectively analyze the data of GC patients who underwent Billroth II (B-II) or Rouxen-Y (R-Y) reconstruction after laparoscopic gastrectomy from 2 institutions (Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, and HwaMei Hospital, University of Chinese Academy of Sciences). The DSL risk factors were analyzed using univariate and multivariate analysis regression. Results: A total of 810 patients were eligible for our analysis (426 with R-Y, 384 with B-II with Braun). Eleven patients had DSL (1.36%). Body mass index (BMI), elevated preoperative C-reactive protein (CRP) level, and unreinforced duodenal stump were the independent risk factors for DSL. DSL was diagnosed in 2-12 days, with a median of 8 days. Seven patients received conservative treatment, 3 patients received puncture treatment, and only 1 patient required reoperation. All patients recovered successfully after treatment. Conclusions: The risk factors of DSL were BMI ≥24 kg/㎡, elevated preoperative CRP level, and unreinforced duodenal stump. Nonsurgical treatments for DSL are preferred.