• 한국미생물·생명공학회지
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• 제25권2호
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• pp.159-165
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• 1997

We collected 3,237 plant samples, mainly leaves of various trees, from many provinces in Korea and a total of 1,925 Bacillus thuringiensis isolates were obtained and characterized. The isolates were characterized in terms of crystal morphology, PAGE pattern of the toxin proteins, plasmids pattern, biochemical characteristics, and bioassay. The microscopic observation showed that 49.1% of the isolates have bipyramidal shape crystals, 7.1% of spherical shape crystals, 1.4% of rhomboidal shape crystals, and others have small or amorphous inclusions. The insecticidal activities of the spore-crystal mixtures of isolates were tested against Plutella xylostella, Bombyx mori, Culex pipiens, and Agelastica coerulea. Bioassay showed that 51.3% of the isolates were shown to be active; lepidopteran-specific (44.8%), dipteran-specific(4.9%) and coleopteran-specific (1.6%). The remainder(48.8%) did not show any activity against the insects we tested. Interestingly though, some of these non-active isolates were shown to have bipyramidal crystals. By serotyping 22 isolates of our collection, we found that there are various kind of subspecies such as aizawai, amagiens, canadensis, darmstadiensis, galleriae, finitimus, kurstaki, morrisoni and neoleonensis, and three isolates have been classified into a new serotype, H49, and one of them, the type strain, named subsp. muju. From this study it was found that phylloplane is a good source for the isolation of Bacillius thuringiensis, and Bacillus thuringiensis is distributed widely in Korea.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.195-200
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• 2000

Bacillus cereus BS229 was identified as a bacteriocin producer with a bactericidal activity against Bacillus thuringiensis subsp. Thomsoni BR-40. Bacillus cereus BS229 and cerein BS229, named tentatively as the bacteriocin produced by Bacillus cereus BS229, showed a narrow spectrum of actibity against Gram-positive and Gram-negative bacteria, along with yeast and molds. Production of cerein BS229 in a 5-1 fermenter followed typical kinetics of primary metabolite synthesis. The antibacterial activity of cerein BS229 on sensitive indicator cells disappeared completely by ${\alpha}-chmotrypsin$ or proteinase K, which indicates its proteinaceous nature. Cerein BS229 seemed to be very stable throughout the pH range of 2.0 of 9.0 and it was relatively heat labile, despite the fact that bacteriocin activity was still detected after being boied for 30min. Cerein BS229 actibity has been changed with some of the organic solvents such as toluene, ethanol, and chloroform. Direct detection of cerein BS229 actibity on SDS-PAGE suggested that it had an apparent molecular mass of about 8.2 kDa.

• 한국생활과학회지
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• 제17권6호
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• pp.1181-1195
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• 2008

This study based on Sa rye pyun ram(四禮便覽) by Lee Jae which are Chosun Dynasty book about the rules in a wedding ceremony. And the research examine into the understand how to the procedure, the true meanings about korea traditional wedding and general opinion of wedding custom. The instrument is the questionnaires, which is analyzed finally through data processing with 129 of wedding venders. The data analyze by making use of SPSS program were frequency analysis, ANOVA, $x^2$-test, pearson's correlation analysis. The summary of a result is as follows. First, the wedding awareness of wedding venders about Eou hon(議婚) is on a high level but Nab chae(納采) is low. Second, wedding venders are knowing with "Hon suh ji(letter), four season cloth, blue-red satin, shell goods" in a Nab peau(納幣) and with "jujube, beef, chicken, Gu jel pan(accompaniment to a drink), alcohol in a Peau back(幣帛). Third, wedding venders are answered that acceded to "Peau back" and "traditional wedding ceremony' but needed no "gifts & cloths for wedding". Forth, the understanding of wedding procedure (婚禮節次) is on a low, especially Bon ray(本禮).

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.363-366
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• 2004

In order to obtain an industrial strain with higher L(+)-lactic acid yield, the wild type strain Rhizopus oryzae PW352 was mutated by means of Nitrogen ions implantation (l5 Kev, $7.8 \times 10^{14} - 2.08 \times 10^{15} ions/Cm^2$ and two mutants RE3303 and RF9052 were isolated. After 36 h shake-flask cultivation, the concentration of L(+)-lactic acid reached 131-136 g/l, the conversion rate of glucose was as high as 86%-90% and the productivity was 3.61 g/l.h. It was almost a 75% increase in lactic acid production compared with the wild type strain. Maximum fermentation temperature of RF9052 was increased to $45^{\circ}C$ from original $36^{\circ}C$. At the same time, the preferred range of fermentation temperature of RF9052 was broadened compared with PW352.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.677-680
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• 2007

To analyze a cotG-based Bacillus subtilis spore display system directly, $GFP_{UV}$ was expressed on the surface of Bacillus subtilis spores. When $GFP_{UV}$ was fused to the C-terminal of the cotG structural gene and expressed, the existence of a $CotG-GFP_{UV}$ fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This $GFP_{UV}$ displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.

• Agribusiness and Information Management
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• 제4권2호
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• pp.1-13
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• 2012

This study aims to subdivide consumers by attributes determined mainly by consumers of fresh ginseng. It is to compare and analyze the characteristics by cluster, and to deduce the implications on distribution and marketing. For this study, a survey was conducted targeting 250 consumers of fresh ginseng. The factors were deduced through performing the exploratory factor analysis on the results of the survey, and the consumers of fresh ginseng were classified through cluster analysis. As a result of the study, the attributes considered for the purchase of fresh ginseng were condensed to the three factors: physical characteristic factor, safety factor, and cultivation indication information factor. With these as the standard, the consumers of fresh ginseng were subdivided into the three clusters: safety-oriented consumption type, label-centered consumption type, and high involvement consumption type. It was found that there were differences in demographic characteristics and attributes considered for purchase of fresh ginseng by cluster analysis. This study suggests the implications for revitalization of the fresh ginseng industry by subdividing consumers of fresh ginseng and suggesting the characteristics by cluster.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 하계학술대회 논문집 B
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• pp.490-492
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• 1998

An intention of this paper is design of a gyro actuator for the attitude control of an unstructured object. It is well known that the attitude control of an object hanging with wire is not easy using usual actuators. Even though an actuator such as a pan can be used for control of the object, it is difficult to meet a desired control objectives. We, for this reason, propose a gyro actuator for the attitude control of an unstructured object. The proposed gyro actuator consists of two motors. The first motor is responsible to spin the wheel and the second motor is used to turn the outer gimbal. Appling the torque to the second motor, which results in the turn of the outer gimbal, torque about the vertical axis will be obtained while a wheel of the gyro is spinning constantly. This torque is used to control the attitude of the object attached. The aim of this paper is of deriving the transfer function of the actuator and presenting the guideline of the design parameters such as the weight and the dimension of the wheel, motors, and the load capacity. Simulations to the mathematical model which has a state feedback control are conducted to show the validity of the proposed gyro actuator.

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.1-5
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• 1993

The gene for mannose enzyme II of phosphoenolpyruvate-dependent phosphotransferase system from Corynebacterium glutamicum KCTC 1445 was cloned into Escherichia coli ZSC113 using plasmid pBR 322. The recombinant plasmid, designated pCTS3, contained 2.2 kb DNA fragment, and the physical map of the cloned DNA fragment was determined. The E. coli ptsM ptsG mutant transformed with pCTS3 restored glucose and mannose fermentation ability, and grew well on these sugars as the sole carbon source in the minimal medium. The transform ant harboring pCTS3 showed a PTS-mediated repression of growth on maltose by mannose analogue, 2-deoxyglucose. The specificity of the response to 2DG therefore indicates that the cloned DNA fragment carries mannose enzyme II gene.

• BMB Reports
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• 제38권3호
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• pp.366-369
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• 2005

NADPH-cytochrome P450 reductase (CPR) transfers electrons from NADPH to cytochrome P450 and also catalyzes the one-electron reduction of many drugs and foreign compounds. Various spectrophotometric assays have been performed to examine electron-accepting properties of CPR and its ability to reduce cytochrome $b_5$, cytochrome c, and ferricyanide. In this report, reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by CPR has been assessed as a method for monitoring CPR activity. The principle advantage of this substance is that the reduction of MTT can be assayed directly in the reaction medium by a continuous spectrophotometric method. The electrons released from NADPH by CPR were transferred to MTT. MTT reduction activity was then assessed spectrophotometrically by measuring the increase of $A_{610}$. MTT reduction followed classical Michaelis-Menten kinetics ($K_m\;=\;20\;{\mu}M$, $k_{cat}\;=\;1,910\;min^{-1}$). This method offers the advantages of a commercially available substrate and short analysis time by a simple measurement of enzymatic activity of CPR.

• 항공우주기술
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• 제12권1호
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• pp.144-151
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• 2013

다목적실용위성 2호(이하 KOMPSAT-2)는 고해상도 1.0m 흑백영상을 촬영할 수 있는 PAN 카메라와 4.0m 다중파장대의 칼라 영상을 수집할 수 있는 멀티스펙스럴 카메라를 탑재하고 있다. 이를 통하여 취득한 위성영상은 국토관리, 농업, 환경, 해양감시 및 GIS등의 광범위한 분야에 활용되고 있다. 본 연구는 KOMPSAT-2 자료를 이용하여 지상좌표를 해석하는 방법과 해석된 지상좌표의 위치정확도를 KOMPSAT-2 시스템 요구사항인 위치정확도를 만족시키기 위해 현재 적용되고 있는 위치정확도 보정계수 산출절차를 정의하는 것을 목적으로 한다.