• 한국정보처리학회논문지
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• 제6권10호
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• pp.2707-2715
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• 1999

Recently, there have been researches to automate processing and analysing images in the medical field using image processing technique, a fast communication network, and high performance hardware. In this paper, we propose a system to be able to analyze morphological abnormality of red-blood cells for peripheral blood image using image processing techniques. To do this, we segment red-blood cells in the blood image acquired from microscope with CCD camera and then extract UNL fourier features to classify them into 15 classes. We reduce the number of multi-variate features using PCA to construct a more efficient classifier. Our system has the best performance in recognition rate, compared with two other algorithms, LVQ3 and k-NN. So, we show that it can be applied to a pathological guided system.

• 한국지능정보시스템학회:학술대회논문집
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• 한국지능정보시스템학회 2005년도 공동추계학술대회
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• pp.304-308
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• 2005

많은 정보가 저장되어 있는 웹은 더 이상 우리의 삶과 별개로 생각할 수 없는 존재가 되었다. 무수한 정보가 웹상에 존재하게 되어 웹은 커졌지만, 단순한 정보의 저장소로 전락할 위기에 놓여있다. 이러한 거대한 정보 저장소에서 사용자는 원하는 정보를 찾기 위해 많은 시간을 소비해야만 한다. 이러한 문제의 해결을 위해 현재까지 많은 연구가 진행중에 있으며, 본 연구에서는 온톨로지 지술에 기반한 정보검색 시스템을 그 해결방안으로 제안한다. 본 논문에서는 온톨로지 기반의 의미적 정보검색을 위해 요구되는 세부적인 요소 기술들을 소개하고 실제로 이들을 설계하고, 유기적인 결합을 통한 의미적 정보검색 시스템을 설계하였다.

• Earthquakes and Structures
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• 제16권2호
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• pp.129-139
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• 2019

This paper develops a new method for estimating the elastic-plastic buckling strength of the truss structures under the static and seismic loads. Firstly, a new method for estimating the buckling strength of the truss structures was derived based on the buckling strength of the representative member considering the parameters, such as the structure configurations, boundary conditions, etc. Secondly, the new method was verified through the buckling strength estimation and the finite element method (FEM) analysis of the single member models, portal frame models and simple truss models. Finally, the method was applied to evaluate the buckling strength of a simple truss structure under seismic load, and the failure loads between the proposed method and the FEM were analyzed reasonably. The results show that the new method is feasible and reliable for structure engineers to estimate the buckling strengths of the truss structures under the static loads and seismic loads.

• 헤리티지:역사와 과학
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• 제37권
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• pp.169-211
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• 2004

중국(中國) 광서성(廣西省) 전주현(全州縣) 동산요족(東山瑤族)은 한족(漢族)과의 교류가 일찍부터 활발했던 이유로 그 기층을 이루는 민속문화(세시풍속 통과의례 민간신앙 주거민속 농업과 농기구 등)가 대체적으로 한족(漢族)의 영향을 많이 받았다. 그러나 그 기저에는 요족(瑤族)의 전통적인 문화형태를 보존해 오고 있다. 세시풍속 중 모든 요족(瑤族)의 시조인 반고(盤古)의 탄생을 기념하는 명절, 여러 의례 중에 혼례 때 이루어지는 곡가(哭嫁) 같은 노래들, 상례(喪禮)에서의 의례형식, 요족(瑤族)의 신앙생활을 주재하는 사공(師公) 등이 있는 것들이 그러하 다. 또한 주거에 있어서 환경적인 요인과 생활습관에 의해 한족(漢族)의 것과는 부분적으로 다른 차이도 발견된다. 예를 들어 주택이 기본적으로 2층 구조라든가, 상량의식에 사용하는 도구가 틀리다는 점 등이다. 농업과 농기구는 기본적으로 한족(漢族)의 그것과 비슷하며 단지 지역적 차이로 인한 약간의 변형이 있는 정도이다.

• Journal of Information Processing Systems
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• 제12권1호
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• pp.1-34
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• 2016

Gene identification is at the center of genomic studies. Although the first phase of the Encyclopedia of DNA Elements (ENCODE) project has been claimed to be complete, the annotation of the functional elements is far from being so. Computational methods in gene identification continue to play important roles in this area and other relevant issues. So far, a lot of work has been performed on this area, and a plethora of computational methods and avenues have been developed. Many review papers have summarized these methods and other related work. However, most of them focus on the methodologies from a particular aspect or perspective. Different from these existing bodies of research, this paper aims to comprehensively summarize the mainstream computational methods in gene identification and tries to provide a short but concise technical reference for future studies. Moreover, this review sheds light on the emerging trends and cutting-edge techniques that are believed to be capable of leading the research on this field in the future.

• KSBB Journal
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• 제26권3호
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• pp.243-247
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• 2011

Using Bacillus subtilis spore display system, with cotG as an anchoring motif, levansucrase from Zymomonas mobilis, was displayed on the outer surface of Bacillus subtilis spore. Flow cytometry of DB104 (pSDJH-cotG-levU) spore, proved the surface localization of CotG-LevU fusion protein on the spore compared to that of DB104. Enzymatic activity of DB104 (pSDJH-cotG-levU) spore showed more than 1.5 times higher levansucrase specific activity compared to that of the host spore, which is a remarkable increase of enzymatic activity considering the existence of sacA (sucrase) and sacB (levansucrase) in the Bacillus subtilis chromosome. The spore integrity, revealed by sporulation frequency test after heat and lysozyme treatment of spore, did not changed at all in spite of the CotG-LevU fusion protein incorporation into the spore coat layer during spore formation process. These data prove again that Bacillus subtilis spore could be considered as good live immobilization vehicle for efficient bioconversion process.

• Journal of Microbiology and Biotechnology
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• 제18권9호
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• pp.1529-1536
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• 2008

Acetylation of lysine residues in proteins is a reversible and highly regulated posttranslational modification. However, it has not been systematically studied in prokaryotes. By affinity immunoseparation using an anti-acetyllysine antibody together with nano-HPLC/MS/MS, we identified 125 lysine-acetylated sites in 85 proteins among proteins derived from Escherichia coli. The lysine-acetylated proteins identified are involved in diverse cellular functions including protein synthesis, carbohydrate metabolism, the TCA cycle, nucleotide and amino acid metabolism, chaperones, and transcription. Interestingly, we found a higher level of acetylation during the stationary phase than in the exponential phase; proteins acetylated during the stationary phase were immediately deacetylated when the cells were transferred to fresh LB culture medium. These results demonstrate that lysine acetylation is abundant in E. coli and might be involved in modifying or regulating the activities of various enzymes involved in critical metabolic processes and the synthesis of building blocks in response to environmental changes.

• Biomolecules & Therapeutics
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• 제11권3호
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• pp.200-203
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• 2003

The present study was carried out to investigate the potential acute toxicity of bamboo leaf water extract by a single oral dose in Sprague-Dawley rats. Twenty male and female rats aged 5 weeks were randomly assigned to four groups of 5 rats each and were administered singly by gavage at dose levels of 0, 1250, 2500, or 5000 mg/kg body weight. Mortalities, clinical findings, and body weight changes were monitored for the l4-day period following the administration. At the end of 14-day observation period, all animals were sacrificed and complete gross postmortem examinations were performed. Throughout the study period, no treatment-related deaths were observed. There were no adverse effects on clinical signs, body weight, and gross finding at any dose tested. The results showed that the single oral administration of bamboo leaf water extract did not induce any toxic effect at a dose level of below 5000 mg/kg in rats and that the minimal lethal dose were considered to be over 5000 mg/kg body weight for both sexes.

• Journal of Microbiology and Biotechnology
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• 제19권5호
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• pp.495-501
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• 2009

A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and $\beta$-galactosidase as model proteins were fused to the C-terminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1127-1134
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• 2009

Escherichia coli excretes acetate during aerobic growth on glycolytic carbon sources, which has been explained as an overflow metabolism when the carbon flux into the cell exceeds the capacity of central metabolic pathways. Nonacetogenic growth of E. coli on gluconeogenic carbon sources like succinate or in carbon-limited slow growth conditions is believed an evidence for the explanation. However, we found that a strain defected in the acs (acetyl Co-A synthetase) gene, the product of which is involved in scavenging acetate, accumulated acetate even in succinate medium and in carbon-limited low growth rate condition, where as its isogenic parental strain did not. The acs promoter was inducible in noncatabolite repression condition, whereas the expression of the ackA-pta operon encoding acetate kinase and phosphotransacetylase for acetate synthesis was constitutive. Results in this study suggest that E. coli excretes and scavenges acetate simultaneously in the carbon-limited low growth condition and in nonacetogenic carbon source, and the activity of the acetate consumption pathway directly affects the accumulation level of acetate in the culture broth.