• Toxicological Research
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• v.16 no.1
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• pp.39-45
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• 2000

This study was carried out to evaluate antitoxic effects Palsun Brewing Water against cadmium by colorimetric methods. The antitoxic activity of Palsun Brewing Water in NIH-3T3 fibroblasts was evaluated by MTT ({3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazoliumbromide} and SRB (sulforhodamine B protein) assays. The light microcopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows: The concentration of 10-2 mg/ml of Palsun Brewing Water was shown significant antitoxic activity against E. coli endotoxin and Salmonella endotoxin. The number of NIH-3T3 fibroblasts were antitoxin and tend to regenerate. These results suggest that Palsun Brewing Water retains a potential antitoxic activity.

• YAKHAK HOEJI
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• v.43 no.2
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• pp.173-179
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• 1999

In the present study, we have evaluated cytotoxic effects, antitumor activities and metastasis inhibitory effects of Palsun brewing water in NIH 3T3 cells, human epitheloid carcinoma cells, and human skin melanoma cells. The light microscopic study showed morphological changes, AG-NOR (argyrophylic nucleolar organizer region) by silver chloride stain, and glycoprotein by PAS (periodic acid stain) reaction of the treated cells. Disruptions in cell organelles were determined by colorimetric methods; MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide) and SRB (sulforhodamine B protein) assays. These results suggest that Palsun Brewing Water retains no cytotoxic effects in NIH 3T3 cells and a growth-inhibitory activity in cancer cell lines.