• The Korean Journal of Zoology
• /
• v.38 no.4
• /
• pp.514-522
• /
• 1995

In order to examine the interaction of albumin with the sperm during capacitation in mouse, proteins of cauda epididymal sperm were extracted under various conditions and analyzed with SDS-PAGE. Sperm surface labeling patterms were also examined using fluorochroin~conjugated wheat germ agglutinin (WGA) and bovine serum albumin (BSA). Albumin was detached from the sperm surface during the incubation and seemed to be constituted the major protein components of the conditioned media in which sperm incubated for 90 mm. Detachment of albumin from the sperm was not affected by the Ca2+ in the medium. WGA-FITC labeling confirmed that Triton X-100 permeabilired plasma membrane overlaying the apical segment of sperm head and detached plasma membrane associated proteins having negatively charged glycoconjugates. BSA-FITC labeling of epididymal sperm occurred on the apical segment of periacrosoinal region and postacrosomal region of the head. BSA-FITC labeling was not observed in periacrosoinal region of the sperm treated with Ca2+-ionophore ~3187 (10 MM)~ whereas the postacrosome region of acrosome-reacted sperm was still labeled after the AR. These results suggest that albumin bound to the surface of epididymal sperm is detached during the capacitation process, and it might be involved In physiological change of sperm plasma membrane accompanying the capacitation.

• Microbiology and Biotechnology Letters
• /
• v.25 no.1
• /
• pp.23-29
• /
• 1997

The nucleotide sequence of the estI gene encoding acetylxylan esterase I of Bacillus stearothermophilus was determined and analyzed. The estI gene was found to consist of a 810 base pair open reading frame coding for a polypeptide of 270 amino acids with a deduced molecular weight of 30 kDa. This was in well agreement with the molecular weight (29 kDa) estimated by SDS-PAGE of the purified esterase. The coding sequence was preceded by a putative ribo some binding site 10 bp upsteam of the ATG codon. Further 53 bp upstream, the transcription initiation signals were identified. The putative $_{-}$10 sequence (TCCAAT) and $_{-}$35 seqence (TTGAAT) corresponded closely to the respective consensus sequences for the Bacillus subtiis major RNA polymerase. The G+C content of the coding region of the estI was 51% whereas that of the third position of codone was 60.2%. The N-terminal amino acid sequence of the EstI deduced from the nucleotide sequence perfectly matched the corresponding region of the purified esterase described previously. Comparison with the amino acid sequence of other esterases and lipases reported so far allowed us to identify a sequence, GLSMG at positions 123 to 127 of the EstI which was reported to be the highly conserved active site sequence for those enzymes. The nucleotide sequence of the estI revealed 55.7% homology to that of the xylC coding for the acetylxylan esterase of Caldocellum saccharolyticum.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.10 no.4
• /
• pp.316-324
• /
• 2010

In this paper, we design a 256 kbits EEPROM for a MCU (Microcontroller unit) with the wide voltage range of 1.8 V to 5.5 V. The memory space of the EEPROM is separated into a program and data region. An option memory region is added for storing user IDs, serial numbers and so forth. By making HPWs (High-voltage P-wells) of EEPROM cell arrays with the same bias voltages in accordance with the operation modes shared in a double word unit, we can reduce the HPW-to-HPW space by a half and hence the area of the EEPROM cell arrays by 9.1 percent. Also, we propose a page buffer circuit reducing a test time, and a write-verify-read mode securing a reliability of the EEPROM. Furthermore, we propose a DC-DC converter that can be applied to a MCU with the wide voltage range. Finally, we come up with a method of obtaining the oscillation period of a charge pump. The layout size of the designed 256 kbits EEPROM IP with MagnaChip's 0.18 ${\mu}m$ EEPROM process is $1581.55{\mu}m{\times}792.00{\mu}m$.

• International Journal of Advanced Culture Technology
• /
• v.7 no.4
• /
• pp.246-254
• /
• 2019

The purpose of this study is to analyze the issues related to urban regeneration project at the present time through surveys and interviews of participants in the urban regeneration leading project of Suncheon city. Most of the comments were related to business fragmentation and things that should be improved in the future. The text network technique is applied to the subject analysis using unstructured text data. As a result of the frequency of appearance and analysis of page rank centrality between words, words of 'parking', 'need', 'lack', 'region' and 'resident' appeared at the top, and the result of analyzing the mediation centrality of key words showed 'culture', 'Need', 'region', 'inflow' and 'lack' appeared at the top. In the network analysis, the most central words appeared, and many words appeared in the important position in the sentence. Text network analysis has provided timely results in terms of sustainability after completion of the Suncheon City Regeneration Leading Project..

• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.554-557
• /
• 2000

Strain JV3 was isolated from commercial Swiss cheese products and identified as a bacteriocin producer, which has bactericidal activity against Leuconostoc mesenteroides KCCM 11324. Strain JW3 was identified tentatively as Lactococcus lactis by the API test. The activity of lacticin JW3, named tentatively as the bacteriocin produced by Lactococcus lactis JW3, was detected during the mid-log growth phase, and reached a maximum during the early stationary phase, and decreased after the late stationary phase. Its antimicrobial activity on sensitive indicator cells was completely disappeared by protease IV. The inhibitory activities of lacticin JW3 were detected during treatments of up to $121\'^{circ}C$ for 15 min. Lacticin JW3 was very stable over a pH range of 2.0 to 9.0 The apparent molecular mass of lacticin JW3 was estimated to be in the region of 3-3.5kDa, which was determined by the direct detection of bactericidal activity after SDS-PAGE.

• BMB Reports
• /
• v.30 no.1
• /
• pp.80-84
• /
• 1997

The synthesis and secretion of human angiogenin in E. coli by the natural leader sequence has been studied. We constructed a recombinant plasmid containing human angiogenin cDNA which encompassed all the coding region including leader sequence required for secretion. The recombinant plasmid was introduced into a suitable E. coli host. The angiogenin was detected in the culture medium and periplasm upon the induction of gene expression. The molecular weight of the secreted angiogenin was identical to that of authentic angiogenin purfied from human plasma when estimated by SDS-PAGE and immunoblotting. showing that the natural leader sequence was recognized and processed by the secretion machinery of E. coli. The angiogenin concentration in the culture medium reached a maximum within 2 h when expressed at $37^{\circ}C$ with 0.02~2 mM IPTG. In contrast, the expression level increased gradually over time up to 11 h at $23^{\circ}C$ with 0.002~2 mM IPTG and at $37^{\circ}C$ with 0.002 mM IPTG.

• ETRI Journal
• /
• v.38 no.1
• /
• pp.52-61
• /
• 2016

A microblog is a service typically offered by online social networks, such as Twitter and Facebook. From the perspective of information dissemination, we define the concept behind a spreading matrix. A new WeiboRank algorithm for identification of key nodes in microblog networks is proposed, taking into account parameters such as a user's direct appeal, a user's influence region, and a user's global influence power. To investigate how measures for ranking influential users in a network correlate, we compare the relative influence ranks of the top 20 microblog users of a university network. The proposed algorithm is compared with other algorithms - PageRank, Betweeness Centrality, Closeness Centrality, Out-degree - using a new tweets propagation model - the Ignorants-Spreaders-Rejecters model. Comparison results show that key nodes obtained from the WeiboRank algorithm have a wider transmission range and better influence.

• Microbiology and Biotechnology Letters
• /
• v.16 no.6
• /
• pp.536-539
• /
• 1988

A mutant of Bacillus subtilis with a defective cdd gene encoding deoxycytidine-cytidine deaminase (EC 3.5.4.5) has been characterized genetically. The genetic lesion, cdd, causing the altered deoxycytidine-cytidine deaminase was mapped at 225 min on the linkage map of B. subtilis by AR9 transduction, Transductional analysis of the cdd region established the gene order in clockwise as trp-lys-cdd-aroD. The cdd gene was linked 72% with the aroD and 20% with the lys.

• Journal of Digital Contents Society
• /
• v.16 no.4
• /
• pp.513-522
• /
• 2015

Various types of digital displays from PC monitor up to smart watch have been developed in response to the dramatic technological progress of electronics. Accordingly, web content creators have the need to provide web contents respectively depending on the different display environments, which increases production time and costs. As a technique for overcoming this, the responsive web design method has been proposed, which means a web page with the size of the screen being adjusted depending on the types of display. Although the responsive web system is increasingly implemented in the region of contents search pages of domestic portals, there are still many cases that contents are implemented separately for PC and for mobile. In this paper, we investigate the responsive types of the web implementation of contents search pages of domestic portal, and propose an efficient new UI for responsive web to individual types of contents search pages which responsive design is not applied to.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.3
• /
• pp.256-260
• /
• 2004

A major challenge facing those involved in the testing of new plant varieties for distinctness, uniformity and stability (DUS) is the need to compare new varieties against all those of common knowlege (reference varieties). One possible approach would be to group new (candidate) varieties and reference varieties using descriptions stored in databases prior to further of official test. testing. This study was carried out to manage a reference variety collection by databasing of hordein profiling. For this purpose, hordein subunits of the 48 National list barley (Hordeum vulgare L) cultivars were analysed. Total 22 of clear scorable hordein subunits were identified from D-subunit to B-subunit region and fifteen different hordein polypeptide patterns were obtained. Based on hordein subunit band pattern, UPGMA cluster analysis was conducted. Forty-eight cultivars were separated into three groups and genetic distance of cluster ranging from 0.55 to 1.00. Hordein subunits have a potential of selecting similar varieties compared with candidate varieties by controlling reference variety collection and playing an important complemental role in cultivar distinctness.