• Current Research on Agriculture and Life Sciences
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• v.14
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• pp.15-28
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• 1996

The effective components of the peony cultivar, Euseongjagyag and Youngcheonjagyag, which were the major cultivars of peony in Kyeongbuk area, were determined with HPLC and TLC. The paeoniflorin content in the root of Euseongjagyag were more than that of Youngcheonjagyag. The root of Euseongjagyag contained much albiflorin and Youngcheonjagyag contained much oxypaeoniflorin in comparision with albiflorin and oxypaeoniflorin contents in both cultivars of peony. Paeoniflorin contents in accordance with peony prodution regions were ranged from 2.15% to 4.08%, and paeoniflorin content of local cultivar of Euseong and that of Geochang were approximatly the same but that of Youngcheon was the lowest. Paeoniflorin content in the 18 accessions of peony cultivar which were collected from Kyeongbuk area and harvested on November 1993, were ranged from 1.41% to 5.30%. The 18 accessions of peony were classified with the HPLC chromatogram pattern of peony root extract into the three groups which composed with Euseong peony group(9 accessions), Youngcheon peony group (4 accessions) and Punggi standard peony group(5 accessions). High content of paeoniflorin WaS contained in peony root harvested in May and November. but low content of paeoniflorin was contained in peony root harvested in March and September.

• Food Science and Preservation
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• v.4 no.1
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• pp.69-75
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• 1997

Peony is a medicinal herb which have utilized widely as chineses medicine. The paeoniflorin is the predominant component In peony root, monoterpene glucoside containing pinane structure. The effective components were extracted with the cold water from the intact peony roots, and effectively extracted with 70% ethanol from the dry powder of peony roots. The changes of paeoniflorin contents were investigated during the drying process of peony roots and processing of peony extract by the heat-treatment. Air-drying was the best condition for the preservation of paeoniflorin content among the drying processes of peony roots. But convective drying at 6$0^{\circ}C$ was recommended for the drying process of peony roots in large scale. The paeoniflorin in peony extracts was not destroyed by the treatment at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 5 hrs, but destroyed 30%, 28% and 40% of paeoniflorin by treatment at 10$0^{\circ}C$ for 5 hrs, 115$^{\circ}C$ and 121$^{\circ}C$ for 10 minutes, respectively. The paeoniflorin was continueously extracted for 4 hrs from the dry pieces of peony roots(0.5$\times$0.5$\times$0.5cm) in boiling water but destroyed gradually after 4 hrs at 10$0^{\circ}C$. Paeoniflorins in 70% ethanol extracts of peony root were not destroyed at all in the process of concentration to dry powder at 60"C on vacuum.cuum.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.249-254
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• 1997

From Korean cultivated peony root, paeoniflorin and albiflorin, which are generally considered to be principal components of peony root, were isolated by silicagel column chromatography. Their structures were identified by spectroscopic methods $(UV,\;FT-IR,\;^1H{\cdot}^{13}C-NMR)$ and their purities were 98% and 93%, respectively. The concentrations of paeoniflorin and albiflorin in ten Korean cultivated peony lines were determined by reverse phase HPLC. The concentrations of paeoniflorin ranged from 1. 56 to 4.04% and those of albiflorin ranged from 0.04 to 1. 98% in ten Korean cultivated lines. In the ten cultivated lines, the concentrations of albiflorin in Punggi lines were higher than other lines.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.211-216
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• 1997

Peony is commonly used as roots that peeled and dried. But intact root contains higher medicinal constituents than peeled root (machine peeling). The yield of roots was also higher in intact roots. Use of intact root saved the labor time for peeling and 15% of root from yield loss by peeling. Paeoniflorin content was different with each zones of root. The content of Paeoniflorin was 5.11% in epidermis and 3.28% in cortex. When peeled peony roots were dried at hot air after briquet fire drying, root color was good for commercialization. But color of intact root was good at hot air drying without briquet fire drying. The required time for drying was longer in drying of intact roots than peeled roots drying.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.392-398
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• 2002

Comparative analysis of paeoniflorin, albiflorin and phenolic compound contents as bioactive components of peony was performed by Reverse Phase-High Performance Liquid Chromatography (RPHPLC) using the four- year-old peony which were different plant parts and pretreatment, such as removing or unremoving the cork layer of peony root before drying. The contents of paeoniflorin, albiflorin, (+)-taxifolin $3-O-{\beta}-D-glucopyranoside$, (+)-catechin and (-)-epicatechin were the highest in rhizome part, but those of gallic acid and benzoic acid in the leaves were higher than other parts. The contents of albiflorin, gallic acid, benzoic acid and (-)-epicatechin in the cork layer were higher than in those of the core, but the contents of paeoniflorin, (+)-taxifolin $3-O-{\beta}-D-glucopyranoside$ and (+)-catechin in the core were higher than those in the cork layer. In general, the rhizome part of peony root has been used only propagation purpose, but this part contained high contents of bioactive component. Therefore, it is needed that medicinal application of rhizome part in peony root was firmly investigated. Also, In the use of peony root for medicinal purpose, the use of peony root with cork layer can be efficient way on the practical use of useful components and the reduction of labor for removing the cork layer.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.193-199
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• 1999

This study was conducted to establish the standard of quality evaluation in Korean cultivated peony root(Paeoniae Radix). The diameter of fresh root and the concentrations of paeoniflorin, abliflorin and five phenolic compounds at different root ages in Euisung cultivar were investigated. The diameters of fresh root were 5.5mm, 10.3mm, 15.6mm and 19.1mm in one-year, two-year, three-year and four-year-old, respectively. It was also found that the diameter of fresh root was uniformly increased with the increase of root age. The concentrations of paeoniflorin, albiflorin, (+) -catechin and benzoic acid in one-year-old peony root(6.44%, 1.55%, 0.80% and 0.36%, respectively) were higher than those in three-(3.49%, 0.62%, 0.43% and 0.26%) or four-year-old(3.28%, 0.47%, 0.34% and 0.20%). The concentrations of gallic acid, (-) -epicatechin and (+) -taxiforin $3-O-{\beta}-D-glucoside$ were higher in three- (0.26%, 0.09% and 0.26%, respectively) or four-year-old (0.26%, 0.10% and 0.29%) than those in one-year-old (0.25 %, 0.08 % and 0.23 %) by contraries. Excepting (-) -epicatecin, the concentrations of paeoniflorin, albiflorin and four phenolic compounds in the root of unremoved cork layer were higher than those in the root of removed cork layer.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.459-464
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• 2002

This experiment was conducted to know the changes of bioactive component content in four-year-old peony (Paeonia lactiflora Pall.) root with various drying methods such as room temperature drying, $50^{\circ}C$ heat-air drying, room temperature drying after $80^{\circ}C$ boiling water treatment and freeze drying, and to establish the optimum drying method for high quality production of Paeoniae Radix. For this purpose, the contents of paeoniflorin, albiflorin and five phenolic compounds (gallic acid, benzoic acid, (+)-catechin, (-)-epicatechin and (+)-taxifolin 3-O-$\beta$-D-glucopyranoside) in peony root with different drying methods were analyzed by RP-HPLC. The contents of paeoniflorin, albiflorin and (+)-taxifolin 3-O-$\beta$-D-glucopyranoside at room temperature drying were higher than in the other drying methods and that of gallic acid at 8$0^{\circ}C$ boiling water treatment was the highest among that of all drying methods. In the case of freeze drying, the contents of (+)-catechin, benzoic acid and (-)-epicatechin were the highest among those of all drying methods. As increase of drying and treatment temperature, the contents of paeoniflorin, albiflorin, (+)-taxifolin 3-O-$\beta$-D-glucopyrano-side, (+)-catechin and benzoic acid were decreased.

• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.139-145
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• 2001

Peony has been cultivated as medicinal plant in East and also its beautiful flower was acknowledged over the world. In West, numerous peony varieties were developed for ornamental use but not in the country. Ninety two varieties of ornamental peony were introduced from U.S.A. and investigated in Uisong Medicinal Plant Experiment Station during $1997{\sim}1999$. For most of them, their growth habit were good in the country. Their flower colors were clearer and stems were thicker and stronger than domestic peonies. Some of them showed vigorous growth and made very high yield of root. Most of varieties showed proper content of paeoniflorin, medicinal component of peony, for medicinal use.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.3
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• pp.215-219
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• 2006

This study was conducted to find to change of component by different species, plant parts and growth stage of Paeonia lactiflora Pallas Among the species of peony, the contents of compounds was higher in cultivated peony (P. lactiflora P.) as compared with wild peony (P. japonica M., P. obobata M., P. anomala L.). Amount of methyl gallate was highest in 0.45% at Euisung jakyak. Amount of compounds in peony was the highest in 1.0% at paeoniflorin, followed by methyl gallate, astragalin and kaempferol in order. Contents of compounds with different growth stage were observed highest in April, and showed decreased trend in the later growth stage. Methyl gallate was present in 1.79% at petal, 0.56% at leaf and 0.01% at root, astragalin present at 0.27% at petal, 0.20% at leaf and 0.03% at root, and paeoniflorin present at 0.43% at petal, 1.09% at leaf and 2.52% at root.

• Korean Journal of Medicinal Crop Science
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• v.4 no.1
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• pp.68-73
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• 1996

Stem length, main root length and main root diameter showed rapid increasement in two-years-old and three-years-old peony. And there after, the increasement was almost finished. Occurrence of disease was increasing every cultivated year, especially the development of leaf spot was most serious. In propagation by root dividing method, the radix yield (kg/10a) was increasing every cultivated year. However, the difference of radix yield at four and five-years-old peony were not significant. The radix yield of four-years-old peony was higher 26% than three-years-old one. As cultivated year goes by, content of paeoniflorin was increasing then the content was highest at four-years-old peony (4.06%). In 1995, the content, had no certain tendency, was highest at three-years-old peony (3.14%). At hot air drying, browing of peony radix was increasing every cultivated year. If we consider radix yield and color, three-years-old peony was proper object of harvesting.