• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.292-293
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• 2005

• Proceedings of the Korean Nutrition Society Conference
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• 2003.05a
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• pp.254.1-254.1
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.05a
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• pp.64-64
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• 2004

• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.92-93
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• 2004

• Archives of Pharmacal Research
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• v.28 no.7
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• pp.775-783
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• 2005

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a $KBrO_3$ treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.

• Biomolecules & Therapeutics
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• v.14 no.4
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• pp.216-219
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• 2006

In order to establish the anti-inflammatory cellular mechanism of the paeony root(Paeonia lactiflora, Pall, Paeoniaceae), the constituents including paeoniflorin, albiflorin, (+)-catechin, paeonol, benzoic acid and methyl gallate were evaluated for their effects on arachidonate and NO metabolism. Among the compounds tested, only paeonol weakly inhibited cyclooxygenase-2-mediated $PGE_2$ production from LPS-treated RAW 264.7 cells. (+)-Catechin and methyl gallate weakly inhibited inducible nitric oxide synthase-mediated NO production from the same cell line. In particular, methyl gallate significantly inhibited 5-lipoxygenase from RBL-l cells with an $IC_{50}$ of 8.4 ${\mu}M$. These results suggest that the inhibition of these components on arachidonate and NO metabolism may contribute at least in part to anti-inflammatory mechanism of the paeony root.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.384-394
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• 2021

The aim of this study was to investigate the correlation between soil properties and marker compounds contents of Paeonia lactiflora. The methods of determining marker compounds were validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy and recovery using UPLC analysis. P. lactiflora contained albiflorin at 0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin at 1.98 ± 0.14 ~ 6.67 ± 0.84%. The root dry weight (RDW) of P. lactiflora was 0.06 ± 0.02 ~ 1.27 ± 0.28 kg. The soil properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P₂O₅), exchangeable cation and cation exchange capacity (CEC) were performed following standard analysis manual. The results of correlation analysis between soil properties and growth characteristics, available P₂O₅ was positively coreelated with the RDW of P. lactiflora. On the other hand, the RDW of P. lactiflora showed significantly negative correlation with contents of albiflorin and paeoniflorin. The results of this study was might be help to provide useful information on the establish of standard cultivation by the investigate correlation analysis between growth characteristics and marker compound contents of P. lactiflora.

• Journal of Life Science
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• v.12 no.1
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• pp.19-21
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• 2002

The pathway of embryos formed anther culture in herbaceous peony was influenced by addition of 2,4-D. MS medium with 2,4-Dichlorophenoxy acetic acid (2,4-D) alone did not arise direct embryogenesis, but was proliferate callus. Embryos through calli were produced on medium containing 0.2 mg/1 zeatin or without growth regulators. Direct embryogenesis was obtained from MS basal medium. However, after the anthers were cultured on medium with 0.1 mg/1 2,4-D, 3 g/1 AC, 30 g/1 sucrose, 2 g/1 gelrite for 40 days. Its efficiency (32.3 %) was markedly improved when anthers cultured on medium without 2,4-D. Embryo morphology was also affected by the 2,4-D used in medium. The induction of normal embryos with two cotyledons was higher in the embryos formed through direct embryogenesis than those formed callus. The embryos formed from calli were mainly showed abnormal embryo with one, three, four cotyledons or hors and bowling pin type.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.165-168
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• 1995

The embryos formed from anther culture of peony exhibited divergent morphologies. The frequency of normal embryos with two cotyledons was about two times higher in the embryos formed through direct embryogenesis than those formed from callus. About 69% of the embryos with two cotyledons converted into normal plants, but convention rate of the abnormal embryos with one and three or four cotyledons was only 4 to 9%. The embryos of hem and bowling pin shape did not undergo development into normal plants.

• Current Research on Agriculture and Life Sciences
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• v.12
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• pp.51-55
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• 1994

Pollen microspores isolated from peony anthers were cultured by agarose embedding method in the MS medium with 2,4-D(1mg/l) or phenylacetic acid(1, 10, 100mg/l), and without plant hormone. It was observed that pollen microspores cultured on hormone-free medium were directly developed into embryos. Callus formation was enhanced from microspores which were cultured on medium supplemented with 1mg/l PAA. Embryos were also formed from the calli transferred into the hormone-free medium.