• Title/Summary/Keyword: Packed Cell Volume

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Erythrocyte Sedimentation Rates of Reshuffled Packed Cell Volume in Korean Native Goat (PVC수치와 온도가 한국재래 산양적혈구 침강에 미치는 영향)

  • Lee, Soo Doo;Kim, Young Hong;Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.55-59
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    • 1993
  • The packed cell volume(PCV) of Korean native goat, volume percentage of red blood cell in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and erythrocyte sedimentation rate was measured in Westergren tubes at room temperature ($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The sedimentation rates of red blood cell obtained are summarized as follows. The erythrocyte sedimentation rates of Korean native goat are accelerated more at high temperature than low temperature. The erythrocyte sedimentation rates of reshuffled Korean native goat upon time are almost linear for several hours. The erythrocyte sedimentation rates of Korean native goat are settled faster at low PCV than higher PCV, i. e., there is a reverse relationshif between the erythrocyte sedimentation rate and packed cell volume.

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Studies on the red blood cell sedimention rates in heteroplasma of chicken and goat (닭 및 산양의 이종plasma내에서 적혈구 침강에 대한 연구)

  • Yu, Chang-jun;Lee, Soo-doo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.271-277
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    • 1988
  • In order to study the marked variation of red blood cell sedimentation rate in some species of animals, the packed cell volume, volume percentage of erythrocytes in whole blood, was reshuffled of 20%, 40% and 60% using heteroplasma of chicken and goat, and the red blood cell sedimentation rate was measured in Westergren tubes at $27{\pm}1^{\circ}C$ and $8{\pm}1^{\circ}C$. The results obtained were summarized as follows: 1. The values of packed cell volume(PCV) of goat and chicken were $40.7{\pm}4.1%$ and $30.2{\pm}2.2%$ respectively. 2. The sedimentation rates of reshuffled red blood cell were settled faster at lower PCV than higher PCV, i.${\acute{e}}$. there was a reverse relationship between the sedimention rate and PCV. 3. Red blood cells of chicken settled quickly, where as those of goat settled very slowly. Chicken red blood cell sedimented rapidly even in goat plasma, and goat red blood cell sedimented slowly in chicken plasma. These findings indicate that the plasma is not the only factor determining the rapid red blood cell sedimentation rate of chicken. 4. The sedimentation rate of reshuffled red blood cell of chicken and goat were accelerated at higher temperature than lower temperature.

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Red Cell Sedimentation Rates of Reshuffled Packed Cell Volume in Chicken (PCV수치를 변경시킨 닭적혈구 침강속도)

  • Yu, Chang Jun;Lee, Soo Doo
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.115-118
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    • 1990
  • The packed cell volume(PCV) of chicken, volume percentage of erythrocytes in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and the red blood cell sedimentation rate was measured in Westergren tubes at room temperature($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The results obtained were summarized as follows. 1. The sedimentation rates of chicken red blood cell were settled faster at low PCV than higher PCV, i,e., there was a reverse relationship between the sedimentation rate and PCV. 2. The sedimentation rate of chicken red blood cell was accelerated more at high temperature than low temperature. 3. The sedimentation rate of reshuffled chicken red blood cell by time was almost linear for several hours.

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EFFECTS OF PROLONGED EXPOSURE TO THE SUN ON BODY WATER TURNOVER AND VOLUME OF THE BLOOD IN SWAMP BUFFALOES

  • Chaiyabutr, N.;Buranakarl, C.;Loypetjra, P.;Chanpongsang, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.1
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    • pp.47-52
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    • 1990
  • During prolonged exposure to the sun for 8 h each day for 10 days in which the highest ambient temperature around 14:00 h was $39^{\circ}C$, buffaloes exposed to the sun without shade increased the turnover of body water by 35% and 76% on day 5 and day 10 of exposure respectively. The total body water markedly decreased on day five and this amount was maintained thereafter. Plasma and blood volumes did not change significantly on day five but markedly decreased on day 10. Packed cell volume significantly decreased on day five and day 10 of the exposure period. The reduction of packed cell volume on day 10 coincided with the decrease in total plasma water. On day 10 of the exposure, an increase in the rate of liquid flow from the rumen was noted. It is concluded that on the fifth day of exposure, the increase in the evaporative cooling process was attributed to initial mobilization of water from the intracellular compartment. The reduction of both plasma and cell volumes occurring from day five to day 10 indicated a loss of body water from both intracellular and extracellular compartments.

Mass Production of Mammalian Cells under Chemostat Cultivation (동물세포의 연속 대량생산에 관한 연구)

  • 이현용;강재구;박형환
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.173-177
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    • 1989
  • Cell density was gradually decreased as the dilution rate was increased under chemostat cultivation of HeLa cells. Maxium cell density was maintained at the dilution rate of 0.020 (1/h) which was far less than the wash-out rate of 0.050 (1/h), Maxium cell productivity of 2 (mL of cells/L/h)was obtained at the dilution rate of 0.030 (1/h) by showing the culture also required maintenance period at low dilution rates, whose result meant the deviation of continuous culture theroy. Methods of indirectly measuring cell density have been introduced to represent mammalian cell growth, which are packed cell volume and oxygen uptake rate, and these values showed good linear relationship with actual cell density by having correlation factor of 0.90. Theoretical maximum oxygen yield, $Y_{O2}^{max}$ and maintenance oxygen consumption rate, m$_{O2}$, were estimated as 4.1$\times$10$^5$ (cells/mmole $O_2$) and 10.71$\times$10$^{-9}$ (mmole $O_2$/cells/h) by employing oxygen yield model.

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Effect of Aloe vera on the Blood Components of Cobalt-60 Gamma Irradiated Rats (Aloe vera가 Cobalt-60 gamma선 조사를 받은 쥐의 혈액성분에 미치는 영향)

  • Choi Min-chul
    • Journal of the korean veterinary medical association
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    • v.26 no.1
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    • pp.38-42
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    • 1990
  • The effect of Aloe vera administration(500mg/kg, P.O.) for 21 days on the total white blood cell, packed cell volume, hemoglobin, total protein, serum glutamic pyruvic transaminase and sorbitol dehydrogenase values in cobalt-60 gamma irradiated rats was s

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Genetic Studies on Faecal Egg Counts and Packed Cell Volume Following Natural Haemonchus contortus Infection and Their Relationships with Liveweight in Muzaffarnagari Sheep

  • Yadav, N.K.;Mandal, Ajoy;Sharma, D.K.;Rout, P.K.;Roy, R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.11
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    • pp.1524-1528
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    • 2006
  • A total of 437 animals, comprising lambs aged between 3 and 12 months and adults of either sex of Muzaffarnagari sheep maintained at the Central Institute for Research on Goats, Makhdoom, Farah, Mathura, India were screened to assess the prevalence of Haemonchus contortus infection following natural infection and to identify the various factors affecting faecal egg count (FEC) and packed cell volume (PCV) of ewes and their genetic control. The relationships between FEC, PCV and body weight were also estimated. The prevalence rate for H. contortus infection in the flock under study was 15.7% indicating much lower occurrence of worm infection in lambs up to one year of age. On the other hand, a large proportion i.e., 67.7% of sheep was refractive to natural H. contortus infection. The random effect of sire significantly contributed (p<0.01) variation in log-transformed FEC (LFEC) of ewes. The season of birth had a significant (p<0.01) effect on LFEC of ewes. The lactating ewes had significantly (p<0.01) higher faecal egg counts compared to dry and pregnant ewes. The linear regression effects of the age of ewes on LFEC of animals were significant (p<0.01) in the present study. The heritabilities of LFEC, PCV and body weights of ewes during the course of infection were moderate to high in magnitude and ranged from 0.24 to 0.47. The LFEC of ewes was significantly (p<0.05) and negatively correlated with PCV at both genetic and phenotypic level. The genetic and phenotypic relationships between LFEC and body weights of ewes were -0.26 and -0.06 for this breed. The genetic correlation of PCV and body weight of ewes was positive and high (0.58) and statistically significant (p<0.05) but it was negatively correlated (-0.01) with body weight at the phenotypic level.

EFFECT OF EXPERIMENTAL Haemonchus contortus INFECTION ON HEMOGLOBIN CONCENTRATION AND PACKED CELL VOLUME OF DOES

  • Howlader, M.M.R.;Capitan, S.S.;Eduardo, S.L.;Roxas, N.P.;Sevilla, C.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.5
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    • pp.597-601
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    • 1996
  • Twelve Philippine does of 3.5-5.0 years old were used in this study. They were divided into three groups, $T_1$, $T_2$ and $T_3$. Four animals were randomly allocated to each group. Before infection, they were dewormed and housed in individual pens with concrete floors. They were provided with a uniform management. They were infected orally with a single dose of three levels (0, 15,000 and 30,000) of infective Haemonchus contortus larvae. Blood samples were collected from the jugular vein of each animal at fortnightly intervals for 17 fortnights. Hemoglobin (Hb) concentration was determined using hemoglobinometer and packed cell volume (PCV) by microhematocrit methods. Animals in infected groups showed significantly (p < 0.05) lower hemoglobin values than the control except during fortnight 1 for group 2 and fortnights 1, 2, 5, 6 and 7 for group 3. Hemoglobin concentration did not significantly (p > 0.05) differ between the animals in infected groups throughout the sampling period except fortnight 2. The PCV values of animals in infected groups were significantly (p < 0.05) lower than the control for most of the sampling periods. The PCV values of animals in group 2 did not significantly (p > 0.05) differ from group 3.

Indirect calculation for volume of packed red cell(VPRC) by means of erythrocyte sedimentation rate(ESR) of diluted blood in dogs (개 회석혈의 적혈구침강율(ESR)에 의한 적혈구 침층용적(VPRC)치의 간접계산법)

  • Lee, Bang-whan;Park, Young-jun
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.41-49
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    • 1989
  • The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5% dextrose or 6% sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But, ESR of diluted blood using the 0.9% saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents. Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of$60^{\circ}$-angled-micro-ESR/40 min in the mixture, four parts of 5% dextrose solution and one part of whole blood. In the ESR values of diluted blood with low ratio, 1:1~3:1, $60^{\circ}$-micro-ESR was higher than $60^{\circ}$-Wintrobe-ESR. But, in the diluted blood with high ratio, 4:1~5:1, there was no different ESR values. For an aid of practical use, authers suggested a list of the $60^{\circ}$-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood.

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