• Title/Summary/Keyword: PVS2

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Relationships between Nailfold Plexus Visibility, and Clinical Variables and Neuropsychological Functions in Schizophrenic Patients (정신분열병 환자에서 손톱 주름 총 시도(叢 視度) (Nailfold Plexus Visibility)와 임상양상, 신경심리 기능과의 관계)

  • Kang, Dae-Yeob;Jang, Hye-Ryeon
    • Korean Journal of Biological Psychiatry
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    • v.9 no.1
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    • pp.50-61
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    • 2002
  • Objectives:High nailfold plexus visibility can reflect central nervous system defects as an etiologic factor of schizophrenia indirectly. Previous studies suggest that this visibility is particularly related to the negative symptoms of schizophrenia and frontal lobe deficiency. In this study, we examined the relationships between nailfold plexus visibility, and various clinical variables and neuropsychological functions in schizo-phrenic patients. Methods:Forty patients(21males, 19 females) satisfying the DSM-IV criteria for schizophrenia and thirty eight normal controls(20 males, 18 females) were measured for Plexus Visualization Score(PVS) by using the capillary microscopic examination. For the assessment of psychopathology, process-reactivity, premorbid adjustment, and neuropsychological functions, we used Positive and Negative Syndrome Scale(PANSS), Ullmann-Giovannoni Process-Reactive Questionnaire(PRQ), Phillips Premorbid Adjustment Scale(PAS), Korean Wechsler Adult Intelligence Scale(KWIS), Continuous Performance Test(CPT), Wisconsin Card Sort Test (WCST), and Word Fluency Test. We also collected data about clinical variables. Results:PVS was correlated with PANSS positive symptom score and composite score negatively. There were no correlations between PVS and PRQ score, PAS score and neuropsychological variables respectively. Conclusions:This study showed that nailfold plexus visibility was a characteristic feature in some schizophrenic patients, and that higher plexus visibility was associated with the negative symptoms of schizophrenia. There was no association between plexus visibility and neuropsychological functions.

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Cryopreservation of Citrus limon (L.) Burm. F Shoot Tips Using a Droplet-vitrification Method

  • Yi, Jung-Yoon;Balaraju, Kotnala;Baek, Hyung-Jin;Yoon, Mun-Seop;Kim, Haeng-Hoon;Lee, Young-Yi
    • Korean Journal of Plant Resources
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    • v.31 no.6
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    • pp.684-694
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    • 2018
  • This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: 'Frost Eureka limon' and 'Cook Eureka limon', using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at $25^{\circ}C$ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at $25^{\circ}C$. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at $0^{\circ}C$ or PVS3 at $25^{\circ}C$. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in $\text\tiny{^1/_2}$ MS for 30 min at $25^{\circ}C$. Shoot tips were post-cultured overnight on survival medium and then micrografted onto 'trifoliate orange' (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of 'Frost Eureka limon' and 'Cook Eureka limon', respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at $0^{\circ}C$. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing $\text\tiny{^1/_4}$ ammonium nitrate overnight before micrografting, was the highest (70.3%) in 'Frost Eureka limon'. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance.

Application of Various Types of Surfactants for Improving Hydrophilic Properties of Polyvinylsiloxane Dental Rubber Impression Materials and its Effects on Physical Properties (폴리비닐실록산 치과용 고무인상재의 친수성 향상을 위한 계면활성제의 응용과 물성에 미치 는 영향)

  • Park, Yeong-Joon;Hwang, Moon-Jin;Kim, Min-Kang;Song, Ho-Jun;Ha, Kwang;Kang, Shin-Young
    • Elastomers and Composites
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    • v.43 no.2
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    • pp.88-103
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    • 2008
  • This study was purposed to investigate the effects of surfactants and fillers on physical properties of hydrophilic polyvinylsiloxane dental impression materials (PVS). Incorporation of surfactants enhanced the hydrophilicity of the PVS, however, it induced increased viscosity and permanent deformation ratio, delayed setting, and decreased tensile strength. At high concentrations of surfactant, the tensile strength was observed to decrease significantly due to the internal pore formation. Especially, the hydrophilicity of the PVS was significantly enhanced with the addition of Silwet L-77. However, the viscosity, strain in compression, pore formation, and setting time increased whereas the elastic recovery rate and strength remarkably decreased. The PVS dental materials containing Span 20 showed the lowest degree of viscosity increase, delayed setting, pore formation, and hydrophilicity. The delayed setting, pore formation, and strength decrease caused by the incorporation of surfactant were improved by substituting the crystalline quartz filler with diatomaceous earth while the contact angle of PVS dental materials increased.

Cryopreservation of in Vitro Grown Shoot Tips of Sweet Potato (Ipomoea batatas L.) by the Encapsulation-Vitrification Method

  • Yi, JungYoon;Lee, GiAn;Lee, YoungYi;Gwag, JaeGyun;Son, EunHo;Park, HongJae
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.635-641
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    • 2016
  • Sweet potato (Ipomoea batatas L.) shoot tips grown in vitro were successfully cryopreserved by encapsulation-vitrification. Encapsulated explants are very easily manipulated, due to the relatively large size of the alginate beads, and a large number of samples can be treated simultaneously. In this study, the effects of sucrose preculture, cryoprotectant preculture, and post-warm recovery media on regrowth, following liquid nitrogen (LN) exposure, were investigated to establish an efficient encapsulation-vitrification protocol for sweet potato. Shoot tips of plants grown in vitro were precultured in 0.3 M sucrose for 2 d before encapsulation. Encapsulated shoot tips were pre-incubated in liquid MS (Murashige and Skoog) medium containing 0.5 M sucrose for 16 h, before preculturing in sucrose-enriched medium (0.7 M sucrose) for 8 h. Shoot tips were osmoprotected with 35% plant vitrification solution 3 (PVS3) for 3 h, before being dehydrated with PVS3 for 2 h at $25^{\circ}C$. The encapsulated and dehydrated shoot tips were transferred to 2 mL cryotubes, suspended in 0.5 mL PVS3, and plunged directly into liquid N. High levels of shoot formation were obtained for the cv. Yeulmi (65.7%) and Yeonwhangmi (80.3%). The regrowth rates of cryopreserved samples in Yeulmi (78.9%) and Yeonwhangmi (91.3%), following culture on ammonium-free MS medium for 5 d, were much higher than those cultured on standard MS medium (65.7% and 80.3%, respectively). This encapsulation-vitrification is a promising method for the long-term preservation of sweet potato.

Economy Analysis and Optimized Capacity Evaluation of Photovoltaic-Related Energy Storage System (태양광 에너지저장장치(ESS) 경제성 분석 및 최적 용량 평가)

  • Lee, Young-Hun;Sung, Tae-Hyun
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.2_2
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    • pp.209-218
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    • 2022
  • The purpose of this study is to analyze an economic assessment of PV-ESS systems based on the power generation performance data of solar power (PV) operating in domestic area, and to calculate the optimal capacity of the energy storage system. In this study, PVs in Gyeonggi-do, Jeollabuk-do, and Gyeongsangbuk-do were targeted, and PVs in this area were assumed to be installed on a general site, and the research was conducted by applying weights based on the facility's capacity. All the analysis was conducted using the actual amount of KPX transactions of PVs in 2019. In order to calculate the optimal capacity of PCS and BESS according to GHI, PV with a minimum/maximum/central value was selected by comparing the solar radiation before the horizontal plane between three years (2017-2019) of the location where PV was installed. As a result of the analysis, in Gyeonggi-do, if the REC weight decreases to 3.4 when there is no change in the cost of installing BESS and PCS, it is more economical to link BESS than PV alone operation of PV. In Jeollabuk-do, it was analyzed that if the REC weight was reduced to 3.6, it was more likely to link BESS than PV operated alone. In Gyeongsangbuk-do, it was analyzed that if the REC weight was reduced to 3.4, it was more likely to link BESS than PV operated alone.

AN EXPERIMENTAL STUDY ON THE ESTABLISHMENT OF PRODUCT VALIDATION SITE AND THE RELATED ACTIVITIES

  • Lee Kwangjae;Kim Younsoo;Kim Yongseung;Hoersch Bianca
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.426-429
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    • 2005
  • In order to evaluate KOrea Multi-Purpose SATellite (KOMPSAT) application products and develop the multi-sensor data application technologies, the Product Validation Site (PVS) will be designed and constructed by Korea Aerospace Research Institute (KARI). Also KARl has a plan for acquisition of multi-sensor data such as ENVISAT ASAR and Project for On Board Autonomy (PROBA) Compact High Resolution Imaging Spectrometer (CHRIS) data through international cooperation with European Space Agency (ESA). These data will be utilized with KOMPSAT-2 Multi-Spectral Camera (MSC) data. KARI and ESA have identified a mutual interest in creating synergy in the joint exploitation of Earth Observation data for science and applications both in Korea and Europe. This paper summarizes the status of joint experimental studies between KARI and ESA for further applications and presents some expected results from related activities.

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Practical Application of Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria × ananassa Duch.) using Droplet-Vitrification

  • Jinjoo Bae;Young-Yi Lee;Jae-Young Song;Jung-Ro Lee;Munsup Yoon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.36-36
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    • 2021
  • Cryopreservation has been broadly used as an efficient method for a long-term conservation for many types of plants especially vegetatively propagated plants. Among several cryopreservation methods, a droplet-vitrification was the most widely applicable and efficient method. Studies have developed protocols for strawberry using droplet-vitrification method and suggested the practical use of the protocol for large number of germplasm with a little modification. In this study, the droplet vitrification method of shoot tip has been tested on 31 accessions provided around the world. Shoot tips were precultured on Murashige and Skoog (MS) liquid medium supplemented with 0.3~0.5M sucrose. Precultured explants were osmoprotected with loading solution, 35% of PVS3 (C4, 17.5% glycerol and 17.5% sucrose) for 40 min and exposed to dehydration solution, PVS3 (B1, 50% glycerol and 50% sucrose) for 60 min. Then, the explants were transferred onto droplets containing 2.5 uL PVS3 on sterilized aluminum foils prior to direct immersion in liquid nitrogen (LN) for 1hr. The cryopreserved shoot tips were rapidly warmed in a water bath at 40C and then unloaded in MS with 0.8M sucrose for 40 min. The shoot tips were cultured in NH4NO3-free MS post culture medium for 2 weeks. Subsequently, the explants were moved to the MS medium for 6 weeks and evaluated the regrowth rate. By this droplet-vitrification protocol, twenty-four accessions showed at least 40% regrowth rate. Out of 24 accessions, 'Nonsan1ho' had the highest regeneration rate of 85.8% and 'Jumbo pureberry' had the lowest with 42.1%.

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Correlation of Oocyte Quality with Fertilization and Embryonic Development in Intracytoplasmic Sperm Injection Treatment (세포질내 정자주입술 시행시 난자의 상태에 따른 수정률과 배아의 발생양상)

  • 임천규;전진현;송상진;김정욱;강인수
    • Development and Reproduction
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    • v.2 no.2
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    • pp.213-221
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    • 1998
  • Intracytoplasmic sperm injection (ICSI) has been widely used to treat couples with infertility due to severely impaired sperm charateristics and for whom conventional in-vitro fertilization (IVF) had failed. The extent to which the morphology of the oocyte at the light microscopy level is related to the results of ICSI vis controversial. In this study, oocytes from 44 patients were reviewed. The ICSI procedure was recorded through CCD camera. The oocytes were divided into five groups according to the presence of cytoplasmic inclusions, the width of perivitelline space (PVS), the presence of cell debris in PVS, the status of first polar body and the flexibility of oolemma. The results showed that the fertilization rate and embryonic development were not associated with the morphological criteria of oocyte. The degeneraton rate of oocytes after ICSI was significantly higher (P<0.001) in the oocytes whose membranes were broken at the moment of insertion (17.7%) than the oocytes whose membranes were broken by aspiration of cytoplasm (1.6%). More oocytes with cytoplasmic inclusions (48.4% vs. 25.1%, p<0.001), wide PVS (35.2% vs. 19.0%, p<0.001), or cell debris in PVS (53.3% vs. 38.4%, p<0.05) were observed in patients with female factor infertility compared to patients with male factor infertility. These results .suggest that the fertilization rate and embryonic development after ICSI are not correlated with oocyte morphology based on the presence of cytoplasmic inclusions, size of PVS, the presence of cell debris in PVS and the status of polar body. And the degeneration rate of oocytes after ICSI was associated with the flexibility of oolemma.

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A Study on the Nucleophilic Addition Reaction of L-cysteine for PVS (PVS 유도체에 대한 L-Cysteine의 친핵성 첨가반응에 관한 연구)

  • Lee, Ki-Chang;Lee, Kwang-Iil;Yoon, Chul-Hun;Hwang, Sung-Kwy;Kong, Seung-Dae
    • Journal of the Korean Applied Science and Technology
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    • v.12 no.2
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    • pp.99-105
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    • 1995
  • Phenylvinylsulfone derivatives were synthesized by Kirners condition. The structure of these compounds were ascertained by means of ultraviolet, melting point, IR and $^1H-NMR$ spectra. The nucleophilic addtion reaction kinetics of L-cysteiene for phenylvinylsulfone was investigated by ultraviolet spectrophotometery in 40% $EtOH-H_2O$ at $25^{\circ}C$. The rate equations which were applied over a wide pH $1.0{\sim}13.0$ range. On the basis of general base catalysis and confirmation of addtion reaction product, the nucleophilic addtion reaction kinetics of L-cysteiene for phenylvinylsulfone were measured by the pH change. From the result of the above caption, a plausible nucleophilic addtion reaction mechanism of L-cysteiene for phenylvinylsulfone was proposed. These compounds may by used ad the starting materials for the preparation of the engineering plastics or the germicide.