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Current Status on Molecular Genetic Study and Comparative Genomic Analysis of Virulence Related Genes in Xanthomonas oryzae pv. oryzae (벼 흰잎마름병균(Xanthomonas oryzae pv. oryzae)의 병원성 유전자의 분자유전학적 연구현황 및 비교유전체 분석)

  • Kang, Hee-Wan;Park, Young-Jin;Lee, Byeong-Moo
    • Korean Journal of Microbiology
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    • v.44 no.1
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    • pp.1-9
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    • 2008
  • Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight on rice. In this paper, current status on molecular genetic study of major virulence genes, hypersensitive response and pathogenicity (hrp), productions of extracellular polysaccharide (EPS), extracellular enzymes and lipopolysaccharides (LPS), avr genes were reviewed. The IS elements with 611 copies including 133 ORF IS were inserted in various regions of the Xoo genome and in expecially regions franking virulence genes. Whole genome sequence of X. oryzae pv. oryzae KACC10331 were used for defining genetic organization of the virulence genes. Futhermore, the virulence genes in Xoo genome were compared to those of other Xanthomonas species in Blast GenBank data base.

Bacterial Canker of Sweet Cherry (Prunus avium L.) Caused by Pseudomonas syringae pv. morsprunorum (Pseudomonas syringae pv. morsprunorum에 의한 양앵두나무 궤양병)

  • Kim, Gyoung-Hee;Nou, Ill-Sup;Hur, Jae-Seoun;Lee, Seung-Don;Koh, Young-Jin
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.80-84
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    • 2005
  • Bacterial canker of sweet cherry (Prunus avium L.) was observed in farmers\' orchards in Goesan, Chungbuk in 2003. Typical canker symptoms occurred on the branches or twigs of sweet cherry in early spring and bacterial exudates oozed out of the cracked barks of diseased trees. Watersoaked brown symptoms appeared on the leaves and severe infection caused thorough defoliation on the branches or twigs of sweet cherry. When severely infected branches or twigs were cut, irregular and rusty-colored symptoms in sapwood and heartwood were clearly found, indicating that they can serve as specific symptoms of bacterial canker of sweet cherry. The causal bacterium responsible for the symptoms was isolated purely from the infected sapwood of sweet cherry. Based on its morphological, physiological and biochemical characteristics, the causal bacterium was identified as Pseudomonas syringae pv. morsprunorum. The bacterium was pathogenic on sweet cherry and Japanese apricot, but not on peach, cherry, and kiwifruit. It is proposed that the disease be named as bacterial canker of sweet cherry.

Occurrence of Bacterial Black Spot on Plum by Xanthomonas aboricola pv. pruni and It's Pathogenicity on Varieties of Some Stone Fruits (Xanthomonas aboricola pv. pruni에 의한 자두 검은점무늬병의 발생과 핵과류 과수 품종에 대한 병원성)

  • Ryu, Young-Hyun;Lee, Joong-Hwan;Kwon, Tae-Young;Kim, Seung-Han;Kim, Dong-Geun
    • Research in Plant Disease
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    • v.18 no.1
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    • pp.40-44
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    • 2012
  • Xanthomonas arboricola pv. pruni is the causal agent of bacterial black spot disease on some stone fruits, e.g. peach, plum and apricot. To evaluate pathogenicity of Xanthomonas arboricola pv. pruni strain from plum, inoculum of the isolated strain was spray inoculated to fruits and leaves of apricot, Japanese apricot and plum. Apricot and Japanese apricot showed severe black spot symptoms on fruits and shot hole symptoms on leaves. In case of apricot, about fifty percent of fruits did not grow and dropped by hypersensitive reaction to spray infection. Plum, cv. Formosa was very susceptible, showing severe black injury lesions on fruits and cankers on leaves and new twigs. On the other hand, plum cv. Daesukjosaeng, was highly resistant. Fruits of several plum cultivars such as Formosa and Chuhee were severely infected at natural infected orchards by X. arboricola pv. pruni. Where as those of Daesukjosaeng, Taeyang, Soldam and Hongrogen were moderately infected.

Optimization of Ramen Flour Formulation by Mixture Experimental Design (혼합물실험설계법에 의한 라면 밀가루 혼합비의 최적화)

  • Park, Hye Ryong;Lee, Seung Ju
    • Food Engineering Progress
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    • v.15 no.4
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    • pp.297-304
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    • 2011
  • Ramen flour formulation was optimized by applying a mixture experimental design. In the optimization, the overall palatability (OP) of cooked ramen and the rheological properties of selected dough were maximized or minimized. Blended ratios of the ingredients such as Dark Northern Spring (DNS), Hard Red Winter (HRW), and Soft White (SW) were designed on a simplex-lattice. Dough rheological properties were measured by Rapid Visco Analyser (RVA), Farinograph, and Extensograph, and the overall palatability by sensory evaluation. Several principal dough rheological properties such as RVA peak viscosity (PV), Farinograph development time (DT), and Extensograph resistance/extensibility after 45 min (R/E 45 min) were selected to influence the overall palatability by canonical correlation analysis (CCA). Goals of the optimization were given as OP maximized, PV maximized, DT minimized, and R/E at 45 min maximized. The optimization results were found to be DNS 33.3%, HRW 33.3%, and SW 33.3% with OP, 5.825; PV, 587.9 cP; DT, 3.1 min; R/E at 45 min, 2.339 BU/mm.

Molecular Marker Development for the Rapid Differentiation of Black Rot Causing Xanthomonas campestris pv. campestris Race 7

  • Yeo-Hyeon Kim;Sopheap Mao;Nihar Sahu;Uzzal Somaddar;Hoy-Taek Kim;Masao Watanabe;Jong-In Park
    • The Plant Pathology Journal
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    • v.39 no.5
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    • pp.494-503
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    • 2023
  • Xanthomonas campestris pv. campestris (Xcc) is a plant pathogen of Brassica crops that causes black rot disease throughout the world. At present, 11 physiological races of Xcc (races 1-11) have been reported. The conventional method of using differential cultivars for Xcc race detection is not accurate and it is laborious and time-consuming. Therefore, the development of specific molecular markers has been used as a substitute tool because it offers an accurate and reliable result, particularly a quick diagnosis of Xcc races. Previously, our laboratory has successfully developed race-specific molecular markers for Xcc races 1-6. In this study, specific molecular markers to identify Xcc race 7 have been developed. In the course of study, whole genome sequences of several Xcc races, X. campestris pv. incanae, X. campestris pv. raphani, and X. campestris pv. vesicatoria were aligned to identify variable regions like sequence-characterized amplified regions and insertions and deletions specific to race 7. Primer pairs were designed targeting these regions and validated against 22 samples. The polymerase chain reaction analysis revealed that three primer pairs specifically amplified the DNA fragment corresponding to race 7. The obtained finding clearly demonstrates the efficiency of the newly developed markers in accurately detecting Xcc race 7 among the other races. These results indicated that the newly developed marker can successfully and rapidly detect Xcc race 7 from other races. This study represents the first report on the successful development of specific molecular markers for Xcc race 7.

Degradation Behavior of Eutectic and Pb-free Solder Plated Ribbon in Crystalline Silicon Photovoltaic Module (유무연 용융도금 리본에 따른 결정질 실리콘 태양전지 모듈 열화거동)

  • Kim, Ju-Hee;Kim, A Yong;Park, Nochang;Ha, Jeong Won;Lee, Sang Guon;Hong, Won Sik
    • Journal of Welding and Joining
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    • v.32 no.6
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    • pp.75-81
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    • 2014
  • Usage of heavy metal element (Pb, Hg and Cd etc.) in electronic devices have been restricted due to the environmental banning of the European Union, such as WEEE and RoHS. Therefore, it is needed to develop the Pb-free solder plated ribbon in photovoltaic (PV) module. This study described that degradation characteristics of PV module under damp heat (DH, $85^{\circ}C$ and 85% R.H.) condition test for 1,000 h. Solar cell ribbons were utilized to hot dipping plate with Pb-free solder alloys. Two types of Pb-free solder plated ribbons, Sn-3.0Ag-0.5Cu (SAC305) and Sn-48Bi-2Ag, and an electroless Sn-40Pb solder hot dipping plated ribbon as a reference sample were prepared to evaluate degradation characteristics. To detect the degradation of PV module with the eutectic and Pb-free solder plated ribbons, I-V curve, electro-luminescence (EL) and cross-sectional SEM analysis were carried out. DH test results show that the reason of maximum power (Pm) drop was mainly due to the decrease fill factor (FF). It was attributed to the crack or oxidation of interface between the cell and the ribbon. Among PV modules with the eutectic and Pb-free solder plated ribbon, the PV module with SAC305 ribbon relatively showed higher stability after DH test than the case of PV module with Sn-40Pb and Sn-48Bi-2Ag solder plated ribbons.

Convergence of the Image Evaluation by BI-RADS Classification in Accordance with Algorithms in DR Mammography (디지털 유방촬영술에서 BI-RADS의 구분에 따른 알고리즘별 영상의 융복합적 평가)

  • Lee, Mi-Hwa
    • Journal of Digital Convergence
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    • v.13 no.9
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    • pp.489-495
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    • 2015
  • Image availability evaluated by the degree of agreement and sensitive using the process improve visualization according to the Algorithm modification in Image Post-Processing. Reliability measured by the Breast Imaging Reporting and Data System. 172 patients visit same period divided by BI-RADS, category five stages, and contents of breast parenchyma into Calcification, Nodule and Mass. Evaluated the TE/PV image reliability, visualization sensitive, agreement of diagnosis. Convergence analysis was an in various fields. According to the result of this research, PV has higher sensitive and accuracy about lesions than TE visual and there is a difference insensitive by contents of breast parenchyma. Therefore, practical use of Algorithm Modification(Tissue Equalization: TE, Premium View: PV) is expected to improve more accurate, useful diagnosis, which has not been easy until now.

Draft genome sequences of Pseudomonas syringae pv. syringae strain WSPS007 causing bacterial shoot blight on apple (사과가지마름병원세균 Pseudomonas syringae pv. syringae WSPS007 균주의 유전체 해독)

  • Lim, Yeon-Jeong;Ryu, Duck Kyu;Kang, Min Kyu;Jeon, Yongho;Park, Duck Hwan
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.80-82
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    • 2019
  • Pseudomonas syringae pv. syringae strain WSPS007 was isolated from infected twigs (Malus pumila) in 2013 in Yeongju, Gyeongbuk Province, Republic of Korea. Here, we report the draft genome sequence of WSPS007 with a chromosome size of 6,238,498 bp (59.04% G+C content). The genome comprises 5,379 CDS, 16 rRNA genes, and 65 tRNA genes. The P. syringae pv. syringae strain WSPS007 genome possesses an ice-nucleating activation (INA) gene and an antifreeze operon that may be related to frost damage by this pathogen. Thus, the genome sequence determined in this study will be useful in understanding the relationship between the outbreak of bacterial shoot blight disease and frost damage in northern Gyeongbuk Province.

Domestic Technical Standards and Performance Test of Photovoltaic PCS for Renewable Energy (신재생에너지용 태양광 PCS 국내 기술기준 및 성능시험)

  • Yoon, Yong-Ho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.3
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    • pp.479-484
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    • 2018
  • This paper describes domestic technical standard of Photovoltaic(PV) PCS(Power Conditioning Systems)-Characteristics of the utility interface. This standard tests utility compatibility and personnel safety and equipment protection of PV inverter performance functions. Especially utility compatibility part includes test items of 1)voltage, current and frequency, 2)normal voltage operating range, 3)DC injection, 4)normal frequency operating range, 5)harmonics and 6)waveform distortion, 7)power factor of PV inverter. Therefore in this paper each test item of domestic technical standard is studied and analyzed and finally full tested by PV inverter performance function.

Installation and Performance Evaluation of 100kWp PV System in Tibet (중국 티베트지역의 100kWp급 태양광발전시스템 실증연구)

  • Kim Seok-Ki;Yun Jae-Ho;Lee Jeong-Chul;Ahn Se-Jin;Yoon Kyung-Hoon;Song Jin-Soo
    • New & Renewable Energy
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    • v.2 no.2 s.6
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    • pp.16-22
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    • 2006
  • This paper present the performance evaluation of PV systems installed at Tibet area of China in order to identity the key factors that determines system operation at a severe climate conditions and promote the cooperation of PV technology between Korea and China. The installed systems consist of 100kW on-grid connected PV systems, BOS(balance of systems), data acquisition and transmission equipments. The Korea side supplied the solar cell, BOS like as inverter, control box and monitoring system. And the Chinese side assembled solar module, constructed site and built control house. It has been shown that the average radiation per monthly from Tibet is 1.5 times larger than that from Mokpo. Also, radiation time from Tibet is 2hour higher than that from Korea.

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