• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.152-159
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• 2014

The regulation of protein tyrosine phosphorylation is mediated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) and is essential for cellular homeostasis. Co-expression of PTKs with PTPs in Pichia pastoris was used to facilitate the expression of active PTKs by neutralizing their apparent toxicity to cells. In this study, the gene encoding phosphatase PTP1B with or without a blue fluorescent protein or peroxisomal targeting signal 1 was cloned into the expression vector pAG32 to produce four vectors. These vectors were subsequently transformed into P. pastoris GS115. The tyrosine kinases EGFR-2 and $PDGFR{\beta}$ were expressed from vector pPIC3.5K and were fused with a His-tag and green fluorescent protein at the N-terminus. The two plasmids were transformed into P. pastoris with or without PTP1B, resulting in 10 strains. The EGFR-2 and $PDGFR{\beta}$ fusion proteins were purified by $Ni^{2+}$ affinity chromatography. In the recombinant P. pastoris, the PTKs co-expressed with PTP1B exhibited higher kinase catalytic activity than did those expressing the PTKs alone. The highest activities were achieved by targeting the PTKs and PTP1B into peroxisomes. Therefore, the EGFR-2 and $PDGFR{\beta}$ fusion proteins expressed in P. pastoris may be attractive drug screening targets for anticancer therapeutics.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.12B
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• pp.1611-1622
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• 2011

The synchronization is one of the important issues for successful operation of femtocell. The synchronization of femtocell is distinctly different from that of larger wireless base stations in a number of important respects such as 1) The femtocell is located in indoor environment which may make it difficult to receive the adequate GNSS signals. 2) The backhaul of femtocell is connected to the public network which may have more PDV than private network. 3) The entire cost of femtocell needs to be very low. In our thesis, we investigate the candidate solutions including AGNSS (Assisted GNSS), NTP (Network Time Protocol), PTP (Precision Timing Protocol) and Cellular Network Listen for indoor timing solution. We propose the AGNSS-PTP Hybrid scheme which can improve time and frequency quality by selecting the better reference between AGNSS and PTP, and cover the standard status which are under discussion from IEEE, ITU-T, and IETF.

• Journal of Pharmacopuncture
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• v.26 no.4
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• pp.319-326
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• 2023

Objectives: The study's goal was to find out whether Chrysanthemum rubellum extract has anti-diabetic properties by concentrating on α-glucosidase and the PTP-1B signaling pathway. C. rubellum flowers were used for extraction using Methanol/water (80/20) as solvent. Methods: LC-MS techniques was used to check the presence of phytoconstituents present in C. rubellum extract. In vitro antidiabetic activity was evaluated using α-glucosidase inhibitory activity and PTP-1B signaling pathway. On Streptozotocin (STZ)-induced rats with diabetes, the in vivo antidiabetic efficacy was assessed using a test for oral glucose tolerance. Results: The phytoconstituents identified in the extract of C. rubellum were apigenin, diosmin, myricetin, luteolin, luteolin-7-glucoside, and Quercitrin as compound 1-6, respectively. Results showed that diosmin exhibited highest α-glucosidase inhibitory activity i.e. 90.39%. The protein level of PTP-1B was lowered and the insulin signalling activity was directly increased by compounds 1-6. The maximum blood glucose levels were seen in all groups' OGTT findings at 30 minutes following glucose delivery, followed by gradual drops. In comparison to the control group, the extract's glucose levels were 141 mg/dL at 30 minutes before falling to 104 mg/dL after 120 minutes. The current study has demonstrated, in summary, that extract with phytoconstituents reduce blood sugar levels in rats. Conclusion: This finding suggests that extract may reduce the chance of insulin resistance and shield against disorders like hyperglycemia.

• ALGAE
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• v.19 no.3
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• pp.271-276
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• 2004

One hundred and fifty four micro algal strains, newly isolated from nationwide freshwaters in Korea, were screened for their anticancer, ant diabetic, and antibiotic activities. The micro algal strains were cultured with different nutritional conditions that were divided into 4 groups as follows; a normal Allen medium, nitrogen (N)-limited medium, phosphorus (P)-limited medium, and N and P-limited medium. Algal biomass was extracted with a mixture of acetone:H₂O (1:1, v:v) and the extracts were used for the screening of bioactive materials. Anticancer, ant diabetic, and antibiotic materials were screened by the methods of vaccinia Hl-related protein tyrosine phosphates (VHR DS-PTPase) inhibition, protein tyrosine phosphates 1B (PTP1B) inhibition, and paper disk. The inhibition activity of VHR DS-PTPase was observed in 18 strains, having a maximum 79% inhibition from Anabaena affinis and the inhibition activity of PTP1B was observed in 9 strains, having a maximum 97% from Sphaerocystis schroeteri. Microcystis aeruginosa incubated in an N and P-limited medium showed antibiotic activity in 8 species out of 13 pathogenic bacteria. As a whole, it seemed that the stressed condition such as N and/or P limitation increased the production of bioactive materials in micro algae.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.852-855
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• 2002

In order to investigate the fracture behaviors(penetration modes) and resistance to penetration during ballistic impact of surface hardening treated Aluminum, Titanium alloy laminates, ballistic tests were conducted. In this paper, Anodized Al 5083-H131 alloy laminates and nitrified Ti (Gr.2) alloy laminates were used to achieve higher surface hardness. Surface hardness test were conducted using a Micro victor's hardness tester and thickness of surface hardening treated specimens was measured by video microscope. Resistance to penetration is determined by the protection ballistic limit($V_50$), a statistical velocity with 50% probability far complete penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, are respectfully observed at and above ballistic limit velocities, as a result of $V_50$ test and Projectile Through Plates (PTP) test methods. PTP tests were conducted with $0^{\circ}$ obliquity at room temperature using 5.56mm ball projectile. $V_50$ tests with $0^{\circ}$ obliquity at room temperature were conducted with projectiles that were able to achieve near or complete penetration during PTP tests.

• Bulletin of the Korean Chemical Society
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• v.28 no.7
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• pp.1141-1150
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• 2007

Molecular modeling study has been performed to assist in the design of PTP1B inhibitors using FlexX. FlexX dockings with 19 test ligands, whose structures have been determined by X-ray crystallography, were successful in reproducing the experimental conformations within the protein. An increase in biological activity is observed as hydrophobic character of formylchromone derivatives increases. Most ligands bind to the activesite regions of the protein successfully in two different score runs. The Drug score run gave better results than the FlexX score run based on the score, rank, binding modes and bond distance of docked structures. Consensus values from the CScore scoring function are between 3 and 5, suggesting that the scoring scheme is reliable. All formylchromone inhibitors considered in this work show unidirectional binding modes in the active site pocket, which is contrary to the bidirectional X-ray results by Malamas et al. and amino acid residues responsible for such orientation are identified to help further development of the inhibitors.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.784-789
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• 2001

In order to investigate the fracture behaviors(penetration modes) and the resistance to penetration during ballistic impact of cold-rolled Al 5052 H34 alloy laminates, anodized Al 5052 H34 alloy laminates, and Al 5052 H34 alloy after cold-rolling, ballistic testing was conducted. In general, superior armor material is brittle materials which have a high hardness. Ballistic resistance of these materials was measured by protection ballistic limit(V50), a statical velocity with 50% probability for incomplete penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, are observed respectfully, resulting from V50 test and Projectile Through Plate(PTP) test at velocities greater than V50. PTP tests were conducted with 0$^{\circ}$obliquity at room temperature using 5.56mm ball projectile. V50 tests with 0$^{\circ}$obliquity at room temperature were also conducted with projectiles that were able to achieve near or complete penetration during PTP tests. Surface Hardness, resistance to penetration, and penetration modes of Al 5052 H34 alloy laminates compared to those of cold-rolled Al 5052 H34 alloy laminates and anodized Al 5052 H34 alloy laminates anodized Al 5052 H34 alloy after cold-rolling.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.507-512
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• 2000

The ob.jective of this study is to determine fracture behaviors(penetrati0n modes) and resistance to penetration duringballistic impact of Al 5052-H34 alloy laminates and anodized Al 5052-H34 alloy laminates. Resistance to penetration is determined by $V_{50}$ ballistic limit, a statical velocity with 50% probability for complete penetration, test method. Fracture behaviors and ballistic tolerance, described by penetration modes, are respectfully observed that result from V50 test and Projectile Through Plates (PTP) test at velocities greater than $V_{50}$. PTP tests were conducted with 0" obliquity at room temperature using 5.56mm ball projectile. $V_{50}$ tests with 0" obliquity at room temperature were conducted with projectiles that were able to achieve near or complete penetration during PTP tests. Surface Hardness, resistance to penetration, and penetration modes of A1 5052-H34 alloy laminates compared to those of anodized Al 5052-H34 alloy laminates.y laminates.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.174-179
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• 2001

In order to investigate the fracture behaviors(perforation modes) and resistance to perforation during ballistic impact of aluminum alloy plate, ballistic tests were conducted. Depth of penetration experiments with 5.56mm-diameter ball projectile launched into 25mm-thickness Al 5052-H34 targets were conducted. A powder gun launched the 3.55g projectiles at striking velocities between 0.6 and 1.0 km/s. radiography of the damaged targets showed different penetration modes as striking velocities increased. Resistance to perforation is determined by the protection ballistic limit($V_{50}$), a statistical velocity with 50% probability for complete perforation. Fracture behaviors and ballistic tolerance, described by perforation modes, are respectfully observed at and above ballistic limit velocities, as a result of $V_{50}$ test and Projectile Through Plates (PTP) test methods. PTP tests were conducted with $0^{\circ}$ obliquity at room temperature using 5.56mm ball projectile. $V_{50}$ tests with $0^{\circ}$ obliquity at room temperature were conducted with projectiles that were able to achieve near or complete perforation during PTP tests. The effect of various impact velocity are studied with depth of penetration.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.567-572
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• 2001

In order to investigate the fracture behaviors(penetration modes) and resistance to penetration during ballistic impact of Titanium alloy laminates and nitrified Titanium alloy laminates which were treated by PVD(Physical Vapor Deposition) method, ballistic tests were conducted. Evaporation, sputtering, and ion plating are three kinds of PVD method. In this research, Ion plating was used to achieve higher surface hardness and surface hardness test were conducted using a Micro vicker's hardness tester. Resistance to penetration is determined by the protection ballistic limit($V_{50}$), a statistical velocity with 50% probability for complete penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, are respectfully observed at and above ballistic limit velocities, as a result of $V_{50}$ test and Projectile Through Plates (PTP) test methods. PTP tests were conducted with $0^{\circ}$ obliquity at room temperature using 5.56mm ball projectile. $V_{50}$ test with $0^{\circ}$ obliquity at room temperature were conducted with projectiles that were able to achieve near or complete penetration during PTP tests. Surface hardness, resistance to penetration, and penetration modes of Titanium alloy laminates are compared to those of nitrified Titanium alloy laminates.